LOCUS       sequence-402872-        9501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encodes a Tet-On Advanced transactivator under control of a 
            constitutive EF1-alpha promoter and confers resistance to the 
            antibiotic G418.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Mossink B, van Rhijn JR, Wang S, Linda K, Vitale MR, Zoller JEM, van
            Hugte EJH, Bak J, Verboven AHA, Selten M, Negwer M, Latour BL, van 
            der Werf I, Keller JM, Klein Gunnewiek TM, Schoenmaker C, Oudakker 
            A, Anania A, Jansen S, Lesch KP, Frega M, van Bokhoven H, Schubert 
            D, Nadif Kasri N
  TITLE     Cadherin-13 is a critical regulator of GABAergic modulation in human
            stem-cell-derived neuronal networks.
  JOURNAL   Mol Psychiatry. 2022 Jan;27(1):1-18. doi: 
            10.1038/s41380-021-01117-x. Epub 2021 May 10.
  PUBMED    33972691
REFERENCE   2  (bases 1 to 9501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41380-021-01117-x"; journalName: "Mol Psychiatry"; date: 
            "2022-01"; volume: "27"; issue: "1"; pages: "1-18"
COMMENT     Sequence Label: pLV[Exp]-EF1A>Tet3G:IRES:Neo
FEATURES             Location/Qualifiers
     source          1..9501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1950..3128
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2181..3119
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3076..3096
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3128..3152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3159..3905
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(3906..3930)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3931..4505,4506..4508,4509..4517)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4098..4115)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4325..4344
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4518..5312
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4518..5312
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4572..4591)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5182..5201
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5313..5333)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5346..5934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5399..5419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5817..5828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6022..6255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6327..6448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6364..6383)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6418..6437
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6488..6623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6643..6662)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6644..6662)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6672..6689)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6672..6688)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6681..6703)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6830..7285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6917..6936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7127..7148
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7311..7415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7416..7484,7485..8276)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7634..7653)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8447..9035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8936..8955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9189..9206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9323..9344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9359..9364,9365..9382,9383..9389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9397..9413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9402..9424
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9421..9437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9421..9437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9456..9476
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9458..9476
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgg gctccggtgc ccgtcagtgg gcagagcgca
     1981 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2041 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2101 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2161 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2221 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2281 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2341 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     2401 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     2461 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     2521 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     2581 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     2641 ctcaagctgg ccggcctgct ctggtgcctg gtctcgcgcc gccgtgtatc gccccgccct
     2701 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     2761 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     2821 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     2881 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     2941 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3001 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3061 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3121 tgtcgtgaca agtttgtaca aaaaagcagg ctgccaccat gtctagactg gacaagagca
     3181 aagtcataaa ctctgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa
     3241 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
     3301 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccactcct
     3361 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct
     3421 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
     3481 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
     3541 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
     3601 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
     3661 cacttctgaa acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
     3721 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
     3781 cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
     3841 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
     3901 ggtaaaccca gctttcttgt acaaagtggg cccctctccc tccccccccc ctaacgttac
     3961 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     4021 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     4081 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     4141 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     4201 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     4261 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     4321 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     4381 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     4441 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     4501 ataatatggc cacaaccatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4561 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4621 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4681 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4741 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4801 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4861 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4921 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4981 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5041 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5101 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5161 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5221 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5281 ccttctatcg ccttcttgac gagttcttct gacaacttta ttatacatag ttgatcaatt
     5341 ccgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5401 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5461 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5521 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5581 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5641 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5701 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     5761 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5821 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5881 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg
     5941 aattcccgcg gttcgcttta agaccaatga cttacaaggc agctgtagat cttagccact
     6001 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6061 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6121 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6181 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6241 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6301 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6361 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6421 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6481 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6541 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6601 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     6661 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6721 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6781 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6841 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6901 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6961 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7021 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7081 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7141 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7201 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7261 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7501 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7561 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7621 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7681 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7741 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7801 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7861 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7921 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7981 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8041 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8101 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8161 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8221 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8281 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagagaga aaggcggaca
     8881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9241 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9301 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9361 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9421 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     9481 aaaagctgga gctgcaagct t
//
