LOCUS       sequence-402873-        8388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector encodes the gene for murine Ascl1 under control of a 
            Tet-controlled promoter and the puromycin resistance gene under 
            control of a constitutive PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Mossink B, van Rhijn JR, Wang S, Linda K, Vitale MR, Zoller JEM, van
            Hugte EJH, Bak J, Verboven AHA, Selten M, Negwer M, Latour BL, van 
            der Werf I, Keller JM, Klein Gunnewiek TM, Schoenmaker C, Oudakker 
            A, Anania A, Jansen S, Lesch KP, Frega M, van Bokhoven H, Schubert 
            D, Nadif Kasri N
  TITLE     Cadherin-13 is a critical regulator of GABAergic modulation in human
            stem-cell-derived neuronal networks.
  JOURNAL   Mol Psychiatry. 2022 Jan;27(1):1-18. doi: 
            10.1038/s41380-021-01117-x. Epub 2021 May 10.
  PUBMED    33972691
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41380-021-01117-x"; journalName: "Mol Psychiatry"; date: 
            "2022-01"; volume: "27"; issue: "1"; pages: "1-18"
COMMENT     Sequence Label: pLV[TetOn]-Puro-TRE3G>mAscl1
FEATURES             Location/Qualifiers
     source          1..8388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1964..1982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2000..2018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2036..2054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2072..2090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2180..2198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2243..2267
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2334..2358
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2359..2368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3061..3085)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3103..3691
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3156..3176)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3574..3585)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3722..4221
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3757..3780)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4154..4173
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4238..4837
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4238..4257)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4734..4754
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4909..5142
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5214..5335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5251..5270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5305..5324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5375..5510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5530..5549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5531..5549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5559..5576)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5559..5575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5568..5590)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5717..6172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5804..5823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6014..6035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6198..6302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6303..6371,6372..7163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6521..6540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7334..7922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7823..7842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8076..8093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8210..8231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8246..8251,8252..8269,8270..8276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8284..8300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8289..8311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8308..8324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8308..8324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8343..8363
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8345..8363
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtt tactccctat cagtgataga
     1981 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     2041 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     2101 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     2161 gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta
     2221 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt
     2281 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaacaagtt
     2341 tgtacaaaaa agcaggctgc caccatggag agctctggca agatggagag tggagccggc
     2401 cagcagccgc agcccccgca gcccttcctg cctcccgcag cctgcttctt tgcgaccgcg
     2461 gcggcggcgg cagcggcggc ggccgcggca gctcagagcg cgcagcagca acagccgcag
     2521 gcgccgccgc agcaggcgcc gcagctgagc ccggtggccg acagccagcc ctcagggggc
     2581 ggtcacaagt cagcggccaa gcaggtcaag cgccagcgct cgtcctctcc ggaactgatg
     2641 cgctgcaaac gccggctcaa cttcagcggc ttcggctaca gcctgccaca gcagcagccg
     2701 gccgccgtgg cgcgccgcaa cgagcgcgag cgcaaccggg tcaagttggt caacctgggt
     2761 tttgccaccc tccgggagca tgtccccaac ggcgcggcca acaagaagat gagcaaggtg
     2821 gagacgctgc gctcggcggt cgagtacatc cgcgcgctgc agcagctgct ggacgagcac
     2881 gacgcggtga gcgctgcctt tcaggcgggc gtcctgtcgc ccaccatctc ccccaactac
     2941 tccaacgact tgaactctat ggcgggttct ccggtctcgt cctactcctc cgacgaggga
     3001 tcctacgacc ctcttagccc agaggaacaa gagctgctgg actttaccaa ctggttctga
     3061 acccagcttt cttgtacaaa gtggtgataa tcgaattccg ataatcaacc tctggattac
     3121 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3181 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3241 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3301 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3361 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3421 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3481 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     3541 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3601 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3661 agtcggatct ccctttgggc cgcctccccg catcgggaat tcccgcggtt cgaattctac
     3721 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     3781 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     3841 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     3901 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     3961 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     4021 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     4081 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     4141 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     4201 tcctcatctc cgggcctttc gacctcacgt ggccaccatg accgagtaca agcccacggt
     4261 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4321 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4381 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4441 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4501 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4561 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4621 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4681 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4741 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     4801 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaggt acctttaaga ccaatgactt
     4861 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4921 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4981 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5041 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5101 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5161 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5221 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5281 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5341 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5401 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5461 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5521 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5581 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5641 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5701 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5761 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5821 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5881 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5941 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6001 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6061 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6121 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6181 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6241 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6301 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6361 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6421 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6481 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6541 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6601 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6661 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6721 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6781 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6841 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6901 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6961 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7021 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7081 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7141 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7441 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7741 cgcttcccga agagagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8101 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8161 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8221 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8281 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8341 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagctt
//
