LOCUS       sequence-402874-        3943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Kim DS, Matsuda T, Cepko CL
  TITLE     A core paired-type and POU homeodomain-containing transcription 
            factor program drives retinal bipolar cell gene expression.
  JOURNAL   J Neurosci. 2008 Jul 30. 28(31):7748-64.
  PUBMED    18667607
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 30. 28(31):7748-64."
COMMENT     Sequence Label: mGluR6 (200bp crit reg)+SV40p-GFP-IRES-AP
FEATURES             Location/Qualifiers
     source          1..3943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..568
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(160..177)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     387..406
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             580..2079
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     primer_bind     complement(2574..2593)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2639..2694
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2693..2712)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3055..3071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3055..3071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3068..3090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3079..3095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3103..3109,3110..3127,3128..3133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3148..3169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3228..3423
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3274..3409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3429..3563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3466..3485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3520..3539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3631..3648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3882..3901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cytccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
       61 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
      121 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
      181 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
      241 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
      301 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
      361 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
      421 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
      481 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
      541 ttttcctttg aaaaacacga tgataatatg gggtcgaggc tgctgctgct gctgctgctg
      601 ggcctgaggc tacagctctc cctgggcatc atcccagttg aggaggagaa cccggacttc
      661 tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc tgcacagaca
      721 gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac ggtgacagct
      781 gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc cctggccatg
      841 gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca tgtgccagac
      901 agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca gaccattggc
      961 ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga ggtcatctcc
     1021 gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac cacacgagtg
     1081 cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg gtactcggac
     1141 gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac gcagctcatc
     1201 tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt tcgcatggga
     1261 accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct ggacgggaag
     1321 aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact
     1381 gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct ctttgagcct
     1441 ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct gatggagatg
     1501 acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct cttcgtggag
     1561 ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact gactgagacg
     1621 atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga ggacacgctg
     1681 agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg
     1741 agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta cacggtcctc
     1801 ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga tgttaccgag
     1861 agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga cgaagagacc
     1921 cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc
     1981 gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct ggagccctac
     2041 accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc ggggcggtcc
     2101 gtggtccccg cgttgcttcc tctgctggcc gggaccctgc tgctgctgga gacggccact
     2161 gctccctgag tgtcccgtcc ctggggctcc tgcttcccca tcccggagtt ctcctgctcc
     2221 ccacctcctg tcgtcctgcc tggcctccag cccgagtcgt catccccgga gtccctatac
     2281 agaggtcctg ccatggaacc ttcccctccc cgtgcgctct ggggactgag cccatgacac
     2341 caaacctgcc ccttggctgc tctcggactc cctaccccaa ccccagggac tgcaggttgt
     2401 gccctgtggc tgcctgcacc ccaggaaagg agggggctca ggccatccag ccaccaccta
     2461 cagcccagtg gggtcgagga tatcgcggcc ggccgcactc ctcaggtgca ggctgcctat
     2521 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     2581 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     2641 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     2701 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     2761 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     2821 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     2881 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     2941 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     3001 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     3061 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3181 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag
     3241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     3421 aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3481 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3541 atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg
     3601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccg
//
