LOCUS       sequence-402875-        7995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to be used as DNA template for in vitro RNA transcription of
            the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid 
            contains optimised 5'UTR and 3'UTR to improve protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Jalil S, Keskinen T, Juutila J, Sartori Maldonado R, Euro L, 
            Suomalainen A, Lapatto R, Kuuluvainen E, Hietakangas V, Otonkoski T,
            Hyvonen ME, Wartiovaara K
  TITLE     Genetic and functional correction of argininosuccinate lyase 
            deficiency using CRISPR adenine base editors.
  JOURNAL   Am J Hum Genet. 2024 Apr 4;111(4):714-728. doi: 
            10.1016/j.ajhg.2024.03.004.
  PUBMED    38579669
REFERENCE   2  (bases 1 to 7995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ajhg.2024.03"; journalName: "Am J Hum Genet"; date: 
            "2024-04-4- 4"; volume: "111"; issue: "4"; pages: "714-728"
COMMENT     Sequence Label: pRN3P_T3_ABE8e_IVT
FEATURES             Location/Qualifiers
     source          1..7995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(65..84)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(66..84)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(91..108)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(91..107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(100..122)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(249..704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(386..407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     598..617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        731..835
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(836..904,905..1696)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1054..1073)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1867..2455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2356..2375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2609..2626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2743..2764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2779..2784,2785..2802,2803..2809)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2817..2833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2822..2844
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2841..2857
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2841..2857
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2878..2896
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           2921..2963
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             3038..3058
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3653..3676,3677..3679,3680..7753)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3653..7753
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7796..7816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     3'UTR           7853..7978
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 aaaaaaaaaa aaaaaccccc cccccccccc ccctgcaggg ccaagtcggc cggtacccaa
       61 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
      121 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      181 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      241 cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      301 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      361 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      421 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      481 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
      541 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
      601 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
      661 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
      721 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      781 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      841 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      901 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      961 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1021 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1081 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1141 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1201 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1261 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1321 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1381 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1441 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1501 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1561 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1621 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1681 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1741 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1801 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1861 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1921 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1981 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2041 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2101 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2161 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2221 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2281 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2341 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2401 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2461 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2521 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2581 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2641 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2701 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     2761 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     2821 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat
     2881 taaccctcac taaagggaac aaaagctgga gctaagcttg cttgttcttt ttgcagaagc
     2941 tcagaataaa cgctcaactt tggcagatct gaattcctgc agcccgggat ccacagccac
     3001 catgaaacgg acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc
     3061 tgaggtggag ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc
     3121 acgggatgag agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg
     3181 cgagggctgg aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatggc
     3241 cctgagacag ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgt
     3301 gacattcgag ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt
     3361 ggtgtttggc gtgaggaact caaaaagagg cgccgcaggc tccctgatga acgtgctgaa
     3421 ctaccccggc atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc
     3481 cgccctgctg tgcgatttct atcggatgcc tagacaggtg ttcaatgctc agaagaaggc
     3541 ccagagctcc atcaactccg gaggatctag cggaggctcc tctggctctg agacacctgg
     3601 cacaagcgag agcgcaacac ctgaaagcag cgggggcagc agcggggggt cagacaagaa
     3661 gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
     3721 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
     3781 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct
     3841 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
     3901 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
     3961 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
     4021 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
     4081 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
     4141 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
     4201 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
     4261 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa
     4321 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
     4381 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
     4441 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
     4501 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
     4561 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
     4621 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
     4681 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
     4741 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
     4801 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac
     4861 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg
     4921 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct
     4981 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg
     5041 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa
     5101 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa
     5161 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct
     5221 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca
     5281 gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct
     5341 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga
     5401 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa
     5461 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac
     5521 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga
     5581 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg
     5641 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa
     5701 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt
     5761 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat
     5821 tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt
     5881 ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc
     5941 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat
     6001 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac
     6061 ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt
     6121 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca
     6181 gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg
     6241 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg
     6301 gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga
     6361 gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac
     6421 ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga
     6481 cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc
     6541 cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc
     6601 ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct
     6661 ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa
     6721 gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa
     6781 ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga
     6841 gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg
     6901 gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg
     6961 cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa
     7021 ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct
     7081 ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct
     7141 ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc
     7201 caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt
     7261 cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa
     7321 cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa
     7381 gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca
     7441 ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga
     7501 cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga
     7561 gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt
     7621 caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga
     7681 cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca
     7741 gctgggaggt gactctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa
     7801 gaagaagagg aaagtctaag taggtgagcg gccgcggatc tggttaccac taaaccagcc
     7861 tcaagaacac ccgaatggag tctctaagct acataatacc aacttacact ttacaaaatg
     7921 ttgtccccca aaatgtagcc attcgtatct gctcctaata aaaagaaagt ttcttcacat
     7981 tctaaaaaaa aaaaa
//
