LOCUS       sequence-402877-        8488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Rakshit A, Holtzen SE, Lo MN, Conway KA, Palmer AE
  TITLE     Human cells experience a Zn(2+) pulse in early G1.
  JOURNAL   Cell Rep. 2023 Jun 16;42(6):112656. doi: 
            10.1016/j.celrep.2023.112656.
  PUBMED    37330912
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-16"; volume: "42(6):112656. doi"; pages: " 
            10.1016/j.celrep.2023.112656"
COMMENT     Sequence Label: PB-NLS-ZapCV2
FEATURES             Location/Qualifiers
     source          1..8488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(260..364)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(390..845)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(527..548)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     739..758
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     972..994
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     986..1003
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     987..1003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1013..1032
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1013..1031
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1061..1095
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1398..1777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1779..2054
                     /label=chicken beta-actin promoter
     intron          2055..3071
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2995..3017
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3079..3098
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3143..3163
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3182..3184,3185..3187,3188..3700)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4088..4822
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     misc_feature    4939..5488
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5083..5100)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5310..5329
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5959..5976)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5965..6189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6236..6252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6260..6276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6284..6290,6291..6308,6309..6314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6329..6350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6584..6646
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6687..6707)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6687..6705)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6726..6742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6750..6766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6774..6780,6781..6798,6799..6804))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6819..6840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6957..6974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7128..7716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7208..7227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7887..8488,1..190,191..259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
      421 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
      481 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
      541 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
      601 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
      661 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
      721 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
      781 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
      841 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      901 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
      961 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     1021 ctcactatag ggcgaattgg ggcgcgccat tctagattaa ccctagaaag atagtctgcg
     1081 taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc gcgaatccgt
     1141 cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa
     1201 tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat gacgcatgat
     1261 tatcttttac gtgactttta agatttaact catacgataa ttatattgtt atttcatgtt
     1321 ctacttacgt gataacttat tatatatata ttttcttgtt atagatatca actagaatgc
     1381 tagcatgggc ccatctcgac attgattatt gactagttat taatagtaat caattacggg
     1441 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1501 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1561 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1621 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1681 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1741 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     1801 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     1861 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgccaggg gggggggggg
     1921 gggggggggg ggggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     1981 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     2041 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     2101 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     2161 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     2221 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     2281 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     2341 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     2401 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     2461 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     2521 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     2581 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     2641 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     2701 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     2761 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     2821 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     2881 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     2941 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     3001 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     3061 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     3121 aattcgcttg gtaccgagca tgcctaaaaa aaaacgtaaa gttgaagatg cttcggatcc
     3181 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3241 cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3301 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3361 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3421 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gtaccatctt
     3481 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3541 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3601 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
     3661 cggcatcaag gcccacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3721 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3781 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3841 cctgctggag ttcgtgaccg ccgcccgcat gcataaaaac aatgacttaa aacacaaatg
     3901 gaaagaatgt cctgagtctt gtagctcact atttgaccta caaagacatc ttttgaagga
     3961 tcatgtctct caagatttca aacaccctat ggaaccatta gctcataatt gggaggactg
     4021 tgatttcctt ggagatgata catgttccat agtgaaccat attaattgtc aacatggtat
     4081 cgagctcatg gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4141 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     4201 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4261 cactctcggc atggacgagc tgtacaaggg tggcagcggt ggcatggtga gcaagggcga
     4321 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4381 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     4441 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     4501 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4561 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4621 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4681 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4741 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     4801 caagatccgc cacaacatcg agtaagaatc ctgcagatat ccagcacagt gggtcgacgg
     4861 gccgcggtaa caattgttaa ctaacttaag ctagcaacgg tttccctcta gcgggatcaa
     4921 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4981 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5041 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5101 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5161 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5221 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5281 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5341 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5401 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5461 tggttttcct ttgaaaaaca cgataatacc atggtcatga aaacatttaa catttctcaa
     5521 caagatctag aattagtaga agtagcgaca gagaagatta caatgcttta tgaggataat
     5581 aaacatcatg tgggagcggc aattcgtacg aaaacaggag aaatcatttc ggcagtacat
     5641 attgaagcgt atataggacg agtaactgtt tgtgcagaag ccattgcgat tggtagtgca
     5701 gtttcgaatg gacaaaagga ttttgacacg attgtagctg ttagacaccc ttattctgac
     5761 gaagtagata gaagtattcg agtggtaagt ccttgtggta tgtgtaggga gttgatttca
     5821 gactatgcac cagattgttt tgtgttaata gaaatgaatg gcaagttagt caaaactacg
     5881 attgaagaac tcattccact caaatatacc cgaaattaaa agtcgagatg catcgatgat
     5941 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6001 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6061 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6121 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6181 gctctatggc ttctgaggcg gaaagaacct gcagcccaag cttggcgtaa tcatggtcat
     6241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatctta aaagttttgt
     6421 tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata
     6481 aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta
     6541 ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg
     6601 catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa tctagtatac
     6661 gcgtttaatt aatccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     6721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7561 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8461 gaatagtgta tgcggcgacc gagttgct
//
