LOCUS       sequence-402878-        8497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Rakshit A, Holtzen SE, Lo MN, Conway KA, Palmer AE
  TITLE     Human cells experience a Zn(2+) pulse in early G1.
  JOURNAL   Cell Rep. 2023 Jun 16;42(6):112656. doi: 
            10.1016/j.celrep.2023.112656.
  PUBMED    37330912
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-16"; volume: "42(6):112656. doi"; pages: " 
            10.1016/j.celrep.2023.112656"
COMMENT     Sequence Label: PB-NLS-ZapCV2-ALA
FEATURES             Location/Qualifiers
     source          1..8497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(260..364)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(390..845)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(527..548)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     739..758
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     972..994
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     986..1003
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     987..1003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1013..1032
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1013..1031
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1061..1095
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1398..1777
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1779..2054
                     /label=chicken beta-actin promoter
     intron          2055..3071
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2995..3017
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3079..3098
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3143..3163
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3182..3184,3185..3187,3188..3700)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4097..4831
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     misc_feature    4948..5497
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5092..5109)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5319..5338
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5968..5985)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5974..6198
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6245..6261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6269..6285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6293..6299,6300..6317,6318..6323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6338..6359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6593..6655
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6696..6716)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6696..6714)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6735..6751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6759..6775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6783..6789,6790..6807,6808..6813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6828..6849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6966..6983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7137..7725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7217..7236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7896..8497,1..190,191..259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
      421 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
      481 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
      541 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
      601 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
      661 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
      721 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
      781 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
      841 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
      901 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
      961 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     1021 ctcactatag ggcgaattgg ggcgcgccat tctagattaa ccctagaaag atagtctgcg
     1081 taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc gcgaatccgt
     1141 cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa
     1201 tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat gacgcatgat
     1261 tatcttttac gtgactttta agatttaact catacgataa ttatattgtt atttcatgtt
     1321 ctacttacgt gataacttat tatatatata ttttcttgtt atagatatca actagaatgc
     1381 tagcatgggc ccatctcgac attgattatt gactagttat taatagtaat caattacggg
     1441 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1501 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1561 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1621 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1681 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1741 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     1801 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     1861 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgccaggg gggggggggg
     1921 gggggggggg gggggggggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     1981 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     2041 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     2101 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     2161 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     2221 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     2281 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     2341 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     2401 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     2461 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     2521 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     2581 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     2641 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     2701 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     2761 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     2821 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     2881 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     2941 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     3001 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     3061 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     3121 aattcgcttg gtaccgagca tgcctaaaaa aaaacgtaaa gttgaagatg cttcggatcc
     3181 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3241 cggcgacgta aacggccaca ggttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3301 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3361 cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3421 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gtaccatctt
     3481 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3541 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3601 caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa
     3661 cggcatcaag gcccacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3721 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3781 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3841 cctgctggag ttcgtgaccg ccgcccgcat gctggccaaa aacaacgacc tgaaggccaa
     3901 gtggaaggag gcccccgaga gcgccagcag cctgttcgac ctgcagcgcg ccctgctgaa
     3961 ggacgccgtg agccaggact tcaaggcccc catggagccc ctggccgcca actgggagga
     4021 cgccgacttc ctgggcgacg acaccgccag catcgtgaac gccatcaacg cccaggccgg
     4081 catcgagctg gagctcatgg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     4141 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     4201 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4261 cgccgggatc actctcggca tggacgagct gtacaagggt ggcagcggtg gcatggtgag
     4321 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4381 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     4441 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4501 caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     4561 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4621 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4681 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4741 gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     4801 ggccaacttc aagatccgcc acaacatcga gtaagaatcc tgcagatatc cagcacagtg
     4861 ggtcgacggg ccgcggtaac aattgttaac taacttaagc tagcaacggt ttccctctag
     4921 cgggatcaat tccgcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4981 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5041 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5101 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5161 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5221 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5281 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5341 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5401 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5461 acggggacgt ggttttcctt tgaaaaacac gataatacca tggtcatgaa aacatttaac
     5521 atttctcaac aagatctaga attagtagaa gtagcgacag agaagattac aatgctttat
     5581 gaggataata aacatcatgt gggagcggca attcgtacga aaacaggaga aatcatttcg
     5641 gcagtacata ttgaagcgta tataggacga gtaactgttt gtgcagaagc cattgcgatt
     5701 ggtagtgcag tttcgaatgg acaaaaggat tttgacacga ttgtagctgt tagacaccct
     5761 tattctgacg aagtagatag aagtattcga gtggtaagtc cttgtggtat gtgtagggag
     5821 ttgatttcag actatgcacc agattgtttt gtgttaatag aaatgaatgg caagttagtc
     5881 aaaactacga ttgaagaact cattccactc aaatataccc gaaattaaaa gtcgagatgc
     5941 atcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6001 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6061 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6121 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6181 atgcggtggg ctctatggct tctgaggcgg aaagaacctg cagcccaagc ttggcgtaat
     6241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6301 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatcttaa
     6421 aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa
     6481 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact
     6541 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc
     6601 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaat
     6661 ctagtatacg cgtttaatta atccagcttt tgttcccttt agtgagggtt aattgcgcgc
     6721 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6781 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6841 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7081 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7141 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7621 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7681 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7741 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7801 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7861 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7921 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7981 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8041 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8101 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8161 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8461 tcattctgag aatagtgtat gcggcgaccg agttgct
//
