LOCUS       sequence-402879-        8508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Rakshit A, Holtzen SE, Lo MN, Conway KA, Palmer AE
  TITLE     Human cells experience a Zn(2+) pulse in early G1.
  JOURNAL   Cell Rep. 2023 Jun 16;42(6):112656. doi: 
            10.1016/j.celrep.2023.112656.
  PUBMED    37330912
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-06-16"; volume: "42(6):112656. doi"; pages: " 
            10.1016/j.celrep.2023.112656"
COMMENT     Sequence Label: PB-NES-ZapCV2-Zinc dead
FEATURES             Location/Qualifiers
     source          1..8508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     439..458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(677..781)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(807..1262)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(944..965)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1156..1175
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1389..1411
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1403..1420
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1404..1420
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1430..1449
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1430..1448
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1478..1512
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1815..2194
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2196..2472
                     /label=chicken beta-actin promoter
     intron          2473..3489
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3413..3435
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3497..3516
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3574..3600
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(3610..3612,3613..3615,3616..4128)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     CDS             4525..5259
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     misc_feature    5376..5925
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5520..5537)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5747..5766
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(6396..6413)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6402..6626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6673..6689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6697..6713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6721..6727,6728..6745,6746..6751))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6766..6787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7021..7083
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7124..7144)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7124..7142)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7163..7179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7187..7203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7211..7217,7218..7235,7236..7241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7256..7277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7394..7411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7565..8153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7645..7664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8324..8508,1..607,608..676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
       61 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      121 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      181 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      241 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
      301 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
      361 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
      421 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
      481 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
      541 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      601 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      661 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
      721 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      781 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
      841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     1021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     1081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     1141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     1201 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     1261 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1321 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1381 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     1441 actatagggc gaattggggc gcgccattct agattaaccc tagaaagata gtctgcgtaa
     1501 aattgacgca tgcattcttg aaatattgct ctctctttct aaatagcgcg aatccgtcgc
     1561 tgtgcattta ggacatctca gtcgccgctt ggagctcccg tgaggcgtgc ttgtcaatgc
     1621 ggtaagtgtc actgattttg aactataacg accgcgtgag tcaaaatgac gcatgattat
     1681 cttttacgtg acttttaaga tttaactcat acgataatta tattgttatt tcatgttcta
     1741 cttacgtgat aacttattat atatatattt tcttgttata gatatcaact agaatgctag
     1801 catgggccca tctcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1861 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1921 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1981 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2041 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2101 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2161 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     2221 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     2281 attttgtgca gcgatggggg cggggggggg ggggggcgcg cgccrggggg gggggggggg
     2341 gggggggggg gggggggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     2401 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     2461 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
     2521 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
     2581 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
     2641 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     2701 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     2761 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     2821 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     2881 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     2941 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     3001 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     3061 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
     3121 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     3181 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     3241 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     3301 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     3361 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     3421 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     3481 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     3541 ttcaacttaa gcttggtacc gagcatgctt caacttcctc ctcttgaacg tcttactctt
     3601 tcggatccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3661 gagctggacg gcgacgtaaa cggccacagg ttcagcgtgt ccggcgaggg cgagggcgat
     3721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3781 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac
     3841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgt
     3901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4021 ctggggcaca agctggagta caactacatc agccacaacg tctatatcac cgccgacaag
     4081 cagaagaacg gcatcaaggc ccacttcaag atccgccaca acatcgagga cggcagcgtg
     4141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4201 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4261 cacatggtcc tgctggagtt cgtgaccgcc gcccgcatgc tggccaaaaa caacgacctg
     4321 aaggccaagt ggaaggaggc ccccgagagc gccagcagcc tgttcgacct gcagcgcgcc
     4381 ctgctgaagg acgccgtgag ccaggacttc aaggccccca tggagcccct ggccgccaac
     4441 tgggaggacg ccgacttcct gggcgacgac accgccagca tcgtgaacgc catcaacgcc
     4501 caggccggca tcgagctgga gctcatggac ggcggcgtgc agctcgccga ccactaccag
     4561 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     4621 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     4681 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtgg cagcggtggc
     4741 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4801 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4861 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4921 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     4981 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     5041 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     5101 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     5161 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     5221 ggcatcaagg ccaacttcaa gatccgccac aacatcgagt aagaatcctg cagatatcca
     5281 gcacagtggg tcgacgggcc gcggtaacaa ttgttaacta acttaagcta gcaacggttt
     5341 ccctctagcg ggatcaattc cgcccccccc ccctaacgtt actggccgaa gccgcttgga
     5401 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     5461 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     5521 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     5581 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     5641 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     5701 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     5761 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     5821 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc
     5881 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga taataccatg gtcatgaaaa
     5941 catttaacat ttctcaacaa gatctagaat tagtagaagt agcgacagag aagattacaa
     6001 tgctttatga ggataataaa catcatgtgg gagcggcaat tcgtacgaaa acaggagaaa
     6061 tcatttcggc agtacatatt gaagcgtata taggacgagt aactgtttgt gcagaagcca
     6121 ttgcgattgg tagtgcagtt tcgaatggac aaaaggattt tgacacgatt gtagctgtta
     6181 gacaccctta ttctgacgaa gtagatagaa gtattcgagt ggtaagtcct tgtggtatgt
     6241 gtagggagtt gatttcagac tatgcaccag attgttttgt gttaatagaa atgaatggca
     6301 agttagtcaa aactacgatt gaagaactca ttccactcaa atatacccga aattaaaagt
     6361 cgagatgcat cgatgatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     6421 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6481 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6541 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6601 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaacctgca gcccaagctt
     6661 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6721 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6781 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     6841 gatcttaaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     6901 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     6961 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     7021 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     7081 gggttaatct agtatacgcg tttaattaat ccagcttttg ttccctttag tgagggttaa
     7141 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagc
//
