LOCUS       sequence-402894-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ADAR2 in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Macbeth MR, Bass BL
  TITLE     Large-scale overexpression and purification of ADARs from 
            Saccharomyces cerevisiae for biophysical and biochemical studies.
  JOURNAL   Methods Enzymol. 2007;424:319-31. doi: 
            10.1016/S0076-6879(07)24015-7.
  PUBMED    17662848
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2007"; volume: "424:319-31. doi"; pages: " 
            10.1016/S0076-6879(07)24015-7"
COMMENT     Sequence Label: pSc-humanADAR2
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(555..1346,1347..1415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1178..1197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1416..1520)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1588..1606
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      1964..3306
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    2296..2343
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        3898..4113
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4114..4917
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(8028..8048)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(8049..8078)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(8052..8081)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             complement(8055..8084)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        8106..8770
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     primer_bind     complement(8119..8142)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    8437..8554
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             10193..10384
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(10378..10400)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10486..10626
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10541..10560
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10641..10658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10812..11016,1..384))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10892..10911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
       61 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      121 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      181 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      241 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      301 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      361 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      421 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      481 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      541 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      601 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      661 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
      721 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      781 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      841 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
      901 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      961 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1021 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1081 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1141 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1201 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1261 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1321 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1381 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1441 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1501 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     1561 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     1621 gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca
     1681 ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa
     1741 tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt
     1801 gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg
     1861 gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat
     1921 acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga
     1981 aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
     2041 aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
     2101 ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
     2161 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
     2221 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
     2281 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     2341 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
     2401 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
     2461 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
     2521 ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta
     2581 ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata
     2641 cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat
     2701 aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca
     2761 atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag
     2821 cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa
     2881 tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag
     2941 agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa
     3001 aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt
     3061 aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt
     3121 gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg
     3181 ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt
     3241 tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc
     3301 ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga
     3361 tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca
     3421 tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc
     3481 tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg
     3541 atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg
     3601 aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag
     3661 tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata
     3721 gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa
     3781 ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatact
     3841 agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga taagcttttc
     3901 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     3961 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     4021 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     4081 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     4141 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     4201 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     4261 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     4321 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     4381 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     4441 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     4501 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     4561 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     4621 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     4681 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     4741 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     4801 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     4861 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     4921 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     4981 tatcagttat tacccgggaa tctcggtcgt aatgattttt ataatgacga aaaaaaaaaa
     5041 attggaaaga aaaagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag
     5101 gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat
     5161 gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat
     5221 tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt
     5281 ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc
     5341 gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg
     5401 atcttgtttt tctcttacta ttgatgtacc attggtttca acttcagatt ttattggaac
     5461 agcatccttc acaacattct tactttccac cttcgtggct tcattaatac caggcatgga
     5521 gcttatctgg tccgttcgag ttttcgacga gtttggagac attctttata gatgtccttt
     5581 ttttttaatg atattcgtta aagaacaaaa agtcaaagca gtttaaccta acacctgttg
     5641 ttgatgctac ttgaaacaag gcttctaggc gaatacttaa aaaggtaatt tcaatagcgg
     5701 tttatatatc tgtttgcttt tcaagatatt atgtaaacgc acgatgtttt tcgcccaggc
     5761 tttatttttt ttgttgttgt tgtcttctcg aagaattttc tcgggcagat ctttgtcgga
     5821 atgtaaaaaa gcgcgtaatt aaactttcta ttatgctgac taaaatggaa gtgatcacca
     5881 aaggctattt ctgattatat aatctagtca ttacgatcct cgagtcaggg cgtgagtgag
     5941 aactggtcct gctcggtggg cttctccacc caggccccca gccccgcctt gatgaaggct
     6001 gtgaacagac gcgccttggc ggcctggtac tcctttgccg ccagcttgga ctcatggtac
     6061 acgttgggtt tggtaatctt ggagcgtagt aagtgggagg gaaccttgcc gtgcacacgc
     6121 atccagcgac agtacaacgc gtgcttacac aggcgggacg cgcggcccag ctcatccttc
     6181 ccagtcgtgg cgttgatgac ctcaatagcg gagtcgccta ccgtccagtt gacactgaag
     6241 ttgggggcct tccctggctg ccgtgcttct gcattgctga tgccactgag caaaggcttg
     6301 ttgagggtgt agagaggtgg caggtcctct atgttggaga tccgctggta catggccctg
     6361 gaaaggtggt ccccgtggta aaggctgccc aggatgatgc tcgagaagta aatgggctcc
     6421 acgaaaatgc tgagcaggga tccctggatg cccaccacgt tccagcgtgc aatcttgtca
     6481 ctgcaggaca tggtgagcag ccgctcccct tgcagcaccc cgtcccacgt ttggatgctc
     6541 gcattggagc gcactggaat cgtcccctca ccagactcta ttttggtccg tagctgtcct
     6601 cttgctttac gatttgggtg tctatctgct ggttcttcca ggattggctc atgtggtgag
     6661 aagattctgg catctccaca gggagaggtg ctgatgtaca gatgaaactg gacattctcc
     6721 ttcagcctaa acccccctcg ctctgatttc tgaaagatgg atcttttttg atcatctttg
     6781 ttatttaagt aaagctcaag ttgtgtataa agaaatctga gcaaggatct ccgagatatt
     6841 atttctgcat ggcagtcatt taatgcaagg ccacgatcac tcatgtattc accattaata
     6901 cattttgttc ctgtagaaac acttatcacc ttggcatctt taacatctgt gcctgttgtc
     6961 atgacgactc cagccagcac ttttctgcga gcgtgagggg aggagaagtt gtcggtcagg
     7021 tcaccaaact tacccaggac caggcgtgag acagcgtcag ctaaaacctg cggtaaatgc
     7081 agctgaagac cctcactggg aataggctgg cgagatggcg tctgatccaa gtgcaagtta
     7141 aaaatggcgg ccagggcaga ctgcgcagcc cgggccttgg caagcttctt gtttctcccc
     7201 gagccttcaa agaactgacc atccacgacc acagacatga cgaagctctt ggcatggctc
     7261 tccccgctct cggagaggaa gtcatacttg agtcctgggc gcagttcgtt caagatcatc
     7321 acgggattct tcccactcgg gggtgggaat ggtggtaaga cagggagagg aggctgggct
     7381 aggctggcag gcaccgggga agcagacaag ctgaggtccc cgctggaact gaaggagtca
     7441 tccccattgg agcccacgta aaagggaggc tccgccttgt caggagtttc aaaaccattg
     7501 aagagcgtgt cagggaagtc ggcctggtca gatgtgaagt ccgtgttgac agacagggtc
     7561 ctccccatgg ccaggtgggc ctcagaggca ttaggaaact gaacgaaaga cctcaaggcc
     7621 ttctcagcag catggagttt tgcctttttc tttgtgggac cagagccctc aaaaacctgg
     7681 ccattcacct ccacagacat gacaaacaaa ggcgcgtgca cgggcccagt ctgggacagg
     7741 agtgtgtact gcaaaccagg cttgatctca ttcagctgca tcagggcgtt cttggggagg
     7801 acgggccctg gtgttttcct ccttttcttc aggcggtact tggagtggcc attgctgccc
     7861 tcctccaggg gccgctttct gccggggcca ccaccacccc cattggagag ctgagagccc
     7921 tcgccaggcc caggtgtgct gccatccttg ggggacacgt tgtccagatt gcggttttcc
     7981 ttcacatcag tgctgctgga actcatgttt tcttcatctt ctatatctcc ctggaaatag
     8041 aggttctcat ggtgatggtg atggtgatgg tgatggtgat ggtgtgacat ggttacggat
     8101 ccggggtttt ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata
     8161 cttttattac atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt attagttaaa
     8221 gtggttatgc agtttttgca tttatatatc tgttaataga tcaaaaatca tcgcttcgct
     8281 gattaattac cccagaaata aggctaaaaa actaatcgca ttatcatcct atggttgtta
     8341 atttgattcg ttcatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat
     8401 aaccataaaa gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca
     8461 tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca cggaggagag tcttccttcg
     8521 gagggctgtc acccgctcgg cggcttctaa tccgtacttc aatatagcaa tgagcagtta
     8581 agcgtattac tgaaagttcc aaagagaagg tttttttagg ctaagataat ggggctcttt
     8641 acatttccac aacatataag taagattaga tatggatatg tatatggata tgtatatggt
     8701 ggtaatgcca tgtaatatga ttattaaact tctttgcgtc catccaaaaa aaaagtaaga
     8761 atttttgaaa attcaatata aatgacagct cagttacaaa gtgaaagtac ttctaaaatt
     8821 gttttggtta caggtggtgc tggatacatt ggttcacaca ctgtggtaga gctaattgag
     8881 aatggatatg actgtgttgt tgctgataac ctgtcgaatt ggtcgaccgt cgaccgatgc
     8941 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     9001 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     9061 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     9121 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     9181 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     9241 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     9301 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     9361 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc
     9421 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     9481 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     9541 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     9601 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     9661 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     9721 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     9781 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     9841 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     9901 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     9961 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
    10021 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
    10081 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
    10141 catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa
    10201 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
    10261 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
    10321 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
    10381 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10441 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
    10501 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
    10561 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    10621 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    10681 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    10741 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    10801 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    10861 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    10921 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    10981 ccttcgggaa gcgtggcgct ttctcatagc tcacgc
//
