LOCUS       sequence-402895-       11700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human ADAR1 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Eggington JM, Greene T, Bass BL
  TITLE     Predicting sites of ADAR editing in double-stranded RNA.
  JOURNAL   Nat Commun. 2011;2:319. doi: 10.1038/ncomms1324.
  PUBMED    21587236
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011"; volume: "2:319. doi"; pages: " 
            10.1038/ncomms1324"
COMMENT     Sequence Label: pSC-humanADAR1
FEATURES             Location/Qualifiers
     source          1..11700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     97..116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     350..367
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    382..522
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(448..467)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     608..630
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(624..815)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        2238..2902
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    2454..2571
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2866..2889
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             2924..2953
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             2954..2974
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(6775..7578)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7579..7794)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      8386..9728
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9349..9396)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(10086..10104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10172..10276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10277..10345,10346..11137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10495..10514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11308..11700,1..196)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
       61 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
      121 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      181 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      241 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      301 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      361 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      421 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      481 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
      541 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      601 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
      661 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
      721 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
      781 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
      841 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
      901 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
      961 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     1021 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     1081 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     1141 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     1201 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     1261 agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg
     1321 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     1381 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     1441 cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga
     1501 ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg
     1561 aggcggcata aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag
     1621 ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg
     1681 cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca
     1741 tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg
     1801 cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag
     1861 cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc
     1921 gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca
     1981 tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     2041 agctgactgg gttgaaggct ctcaagggca tcggtcgacg gtcgaccaat tcgacaggtt
     2101 atcagcaaca acacagtcat atccattctc aattagctct accacagtgt gtgaaccaat
     2161 gtatccagca ccacctgtaa ccaaaacaat tttagaagta ctttcacttt gtaactgagc
     2221 tgtcatttat attgaatttt caaaaattct tacttttttt ttggatggac gcaaagaagt
     2281 ttaataatca tattacatgg cattaccacc atatacatat ccatatacat atccatatct
     2341 aatcttactt atatgttgtg gaaatgtaaa gagccccatt atcttagcct aaaaaaacct
     2401 tctctttgga actttcagta atacgcttaa ctgctcattg ctatattgaa gtacggatta
     2461 gaagccgccg agcgggtgac agccctccga aggaagactc tcctccgtgc gtcctcgtct
     2521 tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc gaacaataaa
     2581 gattctacaa tactagcttt tatggttatg aagaggaaaa attggcagta acctggcccc
     2641 acaaaccttc aaatgaacga atcaaattaa caaccatagg atgataatgc gattagtttt
     2701 ttagccttat ttctggggta attaatcagc gaagcgatga tttttgatct attaacagat
     2761 atataaatgc aaaaactgca taaccacttt aactaatact ttcaacattt tcggtttgta
     2821 ttacttctta ttcaaatgta ataaaagtat caacaaaaaa ttgttaatat acctctatac
     2881 tttaacgtca aggagaaaaa accccggatc cgtaaccatg tcacaccatc accatcacca
     2941 tcaccatcac catgagaacc tctatttcca gggagccgag atcaaggaga aaatctgcga
     3001 ctatctcttc aatgtgtctg actcctctgc cctgaatttg gctaaaaata ttggccttac
     3061 caaggcccga gatataaatg ctgtgctaat tgacatggaa aggcaggggg atgtctatag
     3121 acaagggaca acccctccca tatggcattt gacagacaag aagcgagaga ggatgcaaat
     3181 caagagaaat acgaacagtg ttcctgaaac cgctccagct gcaatccctg agaccagaag
     3241 aaacgcagag ttcctcacct gtaatatacc cacatcaaat gcctcaaata acatggtaac
     3301 cacagaaaaa gtggagaatg ggcaggaacc tgtcataaag ttagaaaaca ggcaagaggc
     3361 cagaccagaa ccagcaagac tgaaaccacc tgttcattac aatggcccct caaaagcagg
     3421 gtatgttgac tttgaaaatg gccagtgggc cacagatgac atcccagatg acttgaatag
     3481 tatccgcgca gcaccaggtg agtttcgagc catcatggag atgccctcct tctacagtca
     3541 tggcttgcca cggtgttcac cctacaagaa actgacagag tgccagctga agaaccccat
     3601 cagcgggctg ttagaatatg cccagttcgc tagtcaaacc tgtgagttca acatgataga
     3661 gcagagtgga ccaccccatg aacctcgatt taaattccag gttgtcatca atggccgaga
     3721 gtttccccca gctgaagctg gaagcaagaa agtggccaag caggatgcag ctatgaaagc
     3781 catgacaatt ctgctagagg aagccaaagc caaggacagt ggaaaatcag aagaatcatc
     3841 ccactattcc acagagaaag aatcagagaa gactgcagag tcccagaccc ccaccccttc
     3901 agccacatcc ttcttttctg ggaagagccc cgtcaccaca ctgcttgagt gtatgcacaa
     3961 attggggaac tcctgcgaat tccgtctcct gtccaaagaa ggccctgccc atgaacccaa
     4021 gttccaatac tgtgttgcag tgggagccca aactttcccc agtgtgagtg ctcccagcaa
     4081 gaaagtggca aagcagatgg ccgcagagga agccatgaag gccctgcatg gggaggcgac
     4141 caactccatg gcttctgata accagcctga aggtatgatc tcagagtcac ttgataactt
     4201 ggaatccatg atgcccaaca aggtcaggaa gattggcgag ctcgtgagat acctgaacac
     4261 caaccctgtg ggtggccttt tggagtacgc ccgctcccat ggctttgctg ctgaattcaa
     4321 gttggtcgac cagtccggac ctcctcacga gcccaagttc gtttaccaag caaaagttgg
     4381 gggtcgctgg ttcccagccg tctgcgcaca cagcaagaag caaggcaagc aggaagcagc
     4441 agatgcggct ctccgtgtct tgattgggga gaacgagaag gcagaacgca tgggtttcac
     4501 agaggtaacc ccagtgacag gggccagtct cagaagaact atgctcctcc tctcaaggtc
     4561 cccagaagca cagccaaaga cactccctct cactggcagc accttccatg accagatagc
     4621 catgctgagc caccggtgct tcaacactct gactaacagc ttccagccct ccttgctcgg
     4681 ccgcaagatt ctggccgcca tcattatgaa aaaagactct gaggacatgg gtgtcgtcgt
     4741 cagcttggga acagggaatc gctgtgtaaa aggagattct ctcagcctaa aaggagaaac
     4801 tgtcaatgac tgccatgcag aaataatctc ccggagaggc ttcatcaggt ttctctacag
     4861 tgagttaatg aaatacaact cccagactgc gaaggatagt atatttgaac ctgctaaggg
     4921 aggagaaaag ctccaaataa aaaagactgt gtcattccat ctgtatatca gcactgctcc
     4981 gtgtggagat ggcgccctct ttgacaagtc ctgcagcgac cgtgctatgg aaagcacaga
     5041 atcccgccac taccctgtct tcgagaatcc caaacaagga aagctccgca ccaaggtgga
     5101 gaacggagaa ggcacaatcc ctgtggaatc cagtgacatt gtgcctacgt gggatggcat
     5161 tcggctcggg gagagactcc gtaccatgtc ctgtagtgac aaaatcctac gctggaacgt
     5221 gctgggcctg caaggggcac tgttgaccca cttcctgcag cccatttatc tcaaatctgt
     5281 cacattgggt taccttttca gccaagggca tctgacccgt gctatttgct gtcgtgtgac
     5341 aagagatggg agtgcatttg aggatggact acgacatccc tttattgtca accaccccaa
     5401 ggttggcaga gtcagcatat atgattccaa aaggcaatcc gggaagacta aggagacaag
     5461 cgtcaactgg tgtctggctg atggctatga cctggagatc ctggacggta ccagaggcac
     5521 tgtggatggg ccacggaatg aattgtcccg ggtctccaaa aagaacattt ttcttctatt
     5581 taagaagctc tgctccttcc gttaccgcag ggatctactg agactctcct atggtgaggc
     5641 caagaaagct gcccgtgact acgagacggc caagaactac ttcaaaaaag gcctgaagga
     5701 tatgggctat gggaactgga ttagcaaacc ccaggaggaa aagaactttt atctctgccc
     5761 agtatgactc gaggatcgta atgactagat tatataatca gaaatagcct ttggtgatca
     5821 cttccatttt agtcagcata atagaaagtt taattacgcg cttttttaca ttccgacaaa
     5881 gatctgcccg agaaaattct tcgagaagac aacaacaaca aaaaaaataa agcctgggcg
     5941 aaaaacatcg tgcgtttaca taatatcttg aaaagcaaac agatatataa accgctattg
     6001 aaattacctt tttaagtatt cgcctagaag ccttgtttca agtagcatca acaacaggtg
     6061 ttaggttaaa ctgctttgac tttttgttct ttaacgaata tcattaaaaa aaaaggacat
     6121 ctataaagaa tgtctccaaa ctcgtcgaaa actcgaacgg accagataag ctccatgcct
     6181 ggtattaatg aagccacgaa ggtggaaagt aagaatgttg tgaaggatgc tgttccaata
     6241 aaatctgaag ttgaaaccaa tggtacatca atagtaagag aaaaacaaga tccacaggac
     6301 gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac
     6361 tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat
     6421 caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat
     6481 atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt
     6541 gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg
     6601 ttagcaattt aactgtgata aactaccgca ttaaagcttt ttctttccaa tttttttttt
     6661 ttcgtcatta taaaaatcat tacgaccgag attcccgggt aataactgat ataattaaat
     6721 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     6781 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     6841 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     6901 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     6961 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     7021 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     7081 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     7141 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     7201 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     7261 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     7321 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     7381 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     7441 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     7501 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     7561 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
     7621 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     7681 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     7741 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagctt
     7801 atcgatgata agctgtcaaa catgagaatt ccacggacta tagactatac tagtatactc
     7861 cgtctactgt acgatacact tccgctcagg tccttgtcct ttaacgaggc cttaccactc
     7921 ttttgttact ctattgatcc agctcagcaa aggcagtgtg atctaagatt ctatcttcgc
     7981 gatgtagtaa aactagctag accgagaaag agactagaaa tgcaaaaggc acttctacaa
     8041 tggctgccat cattattatc cgatgtgacg ctgcagcttc tcaatgatat tcgaatacgc
     8101 tttgaggaga tacagcctaa tatccgacaa actgttttac agatttacga tcgtacttgt
     8161 tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac agatagtata
     8221 tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt atgtatttcg
     8281 gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc atccccggtt
     8341 cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga agcatctgtg
     8401 cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     8461 agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc
     8521 tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     8581 atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa
     8641 gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca
     8701 aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac
     8761 tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt
     8821 ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg
     8881 cattttttca agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac
     8941 tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt
     9001 ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt
     9061 cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga
     9121 taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg
     9181 ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg
     9241 tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt
     9301 tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata
     9361 ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
     9421 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
     9481 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
     9541 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
     9601 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
     9661 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
     9721 attggatcat atgcatagta ccgagaaact agtgcgaagt agtgatcagg tattgctgtt
     9781 atctgatgag tatacgttgt cctggccacg gcagaagcac gcttatcgct ccaatttccc
     9841 acaacattag tcaactccgt taggcccttc attgaaagaa atgaggtcat caaatgtctt
     9901 ccaatgtgag attttgggcc attttttata gcaaagattg aataaggcgc atttttcttc
     9961 aaagctttat tgtacgatct gactaagtta tcttttaata attggtattc ctgtttattg
    10021 cttgaagaat tgccggtcct atttactcgt tttaggactg gttcagaatt cttgaagacg
    10081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
    10141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    10201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    10261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
    10321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
    10381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
    10441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
    10501 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
    10561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
    10621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
    10681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
    10741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    10801 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
    10861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    10921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    10981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    11041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    11101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    11161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    11221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    11461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    11521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    11641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
//
