LOCUS       sequence-402900-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mApple-Talin(1-1973)-FRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Wang Y, Barnett SFH, Le S, Guo Z, Zhong X, Kanchanawong P, Yan J
  TITLE     Label-free Single-Molecule Quantification of Rapamycin-induced 
            FKBP-FRB Dimerization for Direct Control of Cellular 
            Mechanotransduction.
  JOURNAL   Nano Lett. 2019 Oct 9;19(10):7514-7525. doi: 
            10.1021/acs.nanolett.9b03364. Epub 2019 Sep 6.
  PUBMED    31466449
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.9b03364"; journalName: "Nano Lett"; date: 
            "2019-10-9- 9"; volume: "19"; issue: "10"; pages: "7514-7525"
COMMENT     Sequence Label: mApple-TalinN10-FRB
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      592..601
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             598..1305
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1011..1030
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7249..7527
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     polyA_signal    7652..7773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7689..7708)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7743..7762
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7780..8235)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7917..7938)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8129..8148
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8262..8366
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8368..8725
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8368..8559
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8391..8411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8576..8711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8760..9554
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8760..9554
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8814..8833)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9424..9443
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9742..9761)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9786..9833
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10162..10750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10651..10670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agccaccatg
      601 gtgagcaagg gcgaggagaa taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      661 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      721 ccctacgagg cctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc
      781 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggtctacat taagcaccca
      841 gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg
      901 atgaacttcg aggacggcgg cattattcac gttaaccagg actcctccct gcaggacggc
      961 gtgttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1021 cagaagaaga ccatgggctg ggaggcctcc gaggagcgga tgtaccccga ggacggcgcc
     1081 ctgaagagcg agatcaagaa gaggctgaag ctgaaggacg gcggccacta cgccgccgag
     1141 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac
     1201 atcaagttgg acatcgtgtc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1261 gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggtcga cctcgagatg
     1321 gttgcgcttt cgctgaagat tagcattggg aatgtggtga agacgatgca atttgagcca
     1381 tctaccatgg tgtacgacgc ctgccgcatg attcgtgagc ggatcccaga ggccctggct
     1441 ggccctccca acgactttgg gctctttctg tcagatgatg accccaaaaa aggcatctgg
     1501 ctagaggctg ggaaagcttt ggactactac atgctccgaa atggggacac catggagtac
     1561 agaaaaaagc agagacccct gaagatacgg atgttagacg gaacggtgaa gactatcatg
     1621 gtggatgact ccaagaccgt cacagacatg ctcatgacca tttgtgcccg aattggtatc
     1681 accaaccatg atgagtattc actggttcga gagctgatgg aagaaaagaa agatgagggg
     1741 acagggaccc tgagaaaaga caagacccta ctgcgagatg aaaagaagat ggagaagcta
     1801 aagcagaagt tgcacacaga cgatgagttg aactggctgg accatgggcg gacactgagg
     1861 gaacagggag tggaagagca tgagacgttg ctgctgcgga gaaagttctt ctactcagac
     1921 cagaatgtgg attcccgaga ccctgtacag ctgaaccttc tctatgtgca ggcacgagat
     1981 gacatcctga atggctccca tcctgtctcc tttgacaagg cctgtgaatt tgcaggcttc
     2041 cagtgccaga tccagtttgg acctcacaat gaacagaagc acaaggctgg cttccttgac
     2101 ctgaaggact tcctgcccaa ggagtatgtg aagcagaagg gagagcgtaa gatctttcag
     2161 gcacacaaga attgtgggca gatgagtgaa attgaggcca aggtacgcta cgtgaagctg
     2221 gcccgttccc tcaagactta cggtgtctcc ttcttcctag tcaaggaaaa gatgaagggg
     2281 aagaataaac tggtgcccag gctgttgggc atcactaagg agtgtgtgat gcgtgtggat
     2341 gagaagacca aggaggtgat tcaggaatgg agcctcacca acatcaaacg ctgggctgcc
     2401 tctcccaaga gctttactct ggactttgga gactatcagg atggctacta ctcagtacag
     2461 acgacagaag gcgagcagat cgcacagctc attgctggct acatagatat catccttaag
     2521 aagaaaaaaa gcaaggacca ttttgggctg gagggagatg aagagtctac tatgttggag
     2581 gactcagtgt ctcccaaaaa gtcaacagtc cttcagcagc agtacaaccg agtggggaaa
     2641 gtggagcacg gctctgtggc tctgccagcc atcatgcgct ctggagcctc tggtcctgag
     2701 aatttccagg tggggagcat gccacctgcc cagcagcaga tcaccagtgg ccagatgcac
     2761 cgaggacaca tgccacctct gacttcagcc cagcaggcgc tcactggaac cattaactcc
     2821 agcatgcagg ctgtgcaggc tgcccaggcc actctggatg actttgagac tctaccccct
     2881 cttggccagg atgctgcctc caaagcttgg cgtaagaaca agatggatga atcaaagcat
     2941 gagatccact cccaggtaga tgccatcaca gctggcactg cctctgtggt aaacctgaca
     3001 gcaggagatc ctgcagagac agactataca gcagtgggct gtgcagtcac caccatctct
     3061 tccaacctga cggagatgtc ccgtggggtg aagctattgg ctgccctgct ggaggatgaa
     3121 ggcggcaatg gccggcccct tctgcaagca gcaaagggcc ttgcaggggc tgtgtcggaa
     3181 ctgcttcgca gtgctcagcc cgccagcgct gagcctcgtc agaacctgct gcaggcagcc
     3241 gggaacgtgg gccaggccag tggggagctg ttgcagcaaa ttggggaaag tgacactgac
     3301 ccccacttcc aggatgttct aatgcagcta gcaaacgcag tggcaagtgc tgcagccgcc
     3361 ctggtcctca aggccaagag tgtggcccag cgcacagagg attccgggct tcagacccaa
     3421 gtgattgctg cagctacgca atgtgctctg tccacttccc agctggtggc ctgtaccaag
     3481 gtggtagcac ctacaatcag ctcacctgtg tgccaagaac agctagtcga ggctgggcga
     3541 ctggtggcca aagctgtgga gggctgtgtg tctgcctcgc aagcagctac agaagatgga
     3601 cagctgcttc gaggggtagg agcagcagcc acggctgtca cccaggccct caatgagctg
     3661 ctgcagcacg tgaaggccca cgccaccgga gctgggcctg ccggtcggta tgaccaagcc
     3721 actgatacca tcctcaccgt cactgaaaac atcttcagct ccatgggtga tgctggggag
     3781 atggtgcgcc aggcccgcat cctggcccaa gccacctcag acctggtcaa tgctatcaag
     3841 gccgatgctg agggggagag tgatctggag aactctagaa agctcctgag tgctgccaag
     3901 atcctcgctg atgccaccgc caagatggtg gaggcggcca agggagcagc cgcccaccct
     3961 gacagtgagg aacagcagca gcgactgcgt gaagcagctg aggggcttcg catggccacc
     4021 aatgcagctg cgcagaacgc catcaagaag aagttggtgc agcgcctgga gcatgcagcc
     4081 aagcaagctg cagcctctgc aacacagacc attgctgcag cccaacatgc agcctctgcc
     4141 cccaaggcct ctgcgggccc ccagccccta ctggtgcaga gctgtaaggc cgtggcagag
     4201 cagattccgt tgctggtgca gggtgtccga ggaagtcaag ctcagcctga cagccctagt
     4261 gctcagctcg ccctcattgc tgccagccag agcttcctgc agccaggtgg gaagatggtg
     4321 gcagcggcaa aagcctcagt gccaacaatt caggaccagg cttcagcgat gcagctgagt
     4381 cagtgcgcaa agaacctagg cactgccctg gccgagctgc gcactgctgc tcagaaggct
     4441 caggaggcat gtggaccttt ggagatggat tctgcactga gtgttgtaca aaatctagag
     4501 aaagacctac aggaaataaa agcagcagct cgagatggca agcttaagcc cttacctggg
     4561 gaaacaatgg agaagtgtac ccaagatctt ggcaacagca ccaaagcggt gagctctgcc
     4621 atcgccaagc tgctggggga gattgcccag ggcaacgaga actatgcagg tattgcagct
     4681 cgggatgtag ctggtggact aaggtcacta gcccaggctg cgcggggtgt ggctgcgctg
     4741 acatcagatc ctgcagtgca ggccattgtg cttgacacag ccagcgacgt tctggacaag
     4801 gccagcagcc tcattgaaga ggcaaaaaaa gcatctggac acccagggga cccagaaagc
     4861 cagcaaaggc ttgctcaggt agctaaagca gtgacccaag ccctgaaccg ctgtgtcagc
     4921 tgcctgcctg gccaaagaga cgtggataat gccctacgag ccgttggaga tgccagcaag
     4981 cgactcctga gtgacttgct tcctccaagc acggggacat ttcaagaagc acagagccga
     5041 ttgaatgaag ctgcagctgg gttaaatcag gccgccacag aactggtgca agcctcccga
     5101 ggaactcctc aggacctggc tcgggcctcc ggccgatttg gacaggactt cagcaccttc
     5161 ctggaagctg gtgtggagat ggccggacag gccccgagcc aggaggaccg agcccaggtg
     5221 gtgtccaacc tgaagggcat atccatgtct tcaagcaaac ttctccttgc cgctaaggcc
     5281 ttgtccacag accctgcttc tcccaacctc aagagtcagc tggctgcggc tgcccgggca
     5341 gtgacggaca gcatcaacca gctcatcacc atgtgcaccc agcaggcacc tggccagaag
     5401 gagtgtgaca atgcacttcg gcagctagag acagtccgag aactcctgga gaatccagtc
     5461 cagcccatca acgacatgtc ctacttcggt tgcttggaca gtgtcatgga gaactccaag
     5521 gtcctaggtg aggccatgac tggcatctcc caaaatgcca agaatggaaa tctgccggag
     5581 tttggagatg ccattgccac agcctccaag gctctctgtg gcttcacgga ggcagccgca
     5641 caggcagcat atctagttgg tgtctctgac cccaacagcc aagctggaca gcaaggactg
     5701 gtggaaccca cacagtttgc ccgtgcaaac caggcaattc agatggcctg tcagagtctg
     5761 ggggagcctg gctgtaccca ggcccaggtg ttatctgcag ccactattgt agccaaacac
     5821 acatctgcat tgtgtaacag ctgtcgcctg gcttccgcta ggactgccaa tcccactgcc
     5881 aagcgccagt ttgtacagtc agccaaggag gtggccaaca gtacagccaa tcttgtcaag
     5941 accatcaagg cactagatgg ggacttcaca gaagagaacc gtgcccagtg ccgagcagcc
     6001 acagcccctc tgctggaagc tgtggataac ctgagtgcct ttgcctccaa ccctgagttc
     6061 tccagcgtcc ctgcccagat cagccctgag ggcagagcag ccatggagcc cattgtaatc
     6121 tctgctaaga caatgttgga gagtgctgga gggctcatcc agacagctcg ggcgttagca
     6181 gtcaatcccc gagacccccc acgttggtct gtgctagctg gccactctcg gactgtctca
     6241 gattccatca agaaacttat tacaagcatg agggacaaag ccccagggca gctggagtgt
     6301 gagacagcca ttgcggctct gaacagctgc ttgcgggacc tagatcaggc ttcgcttgcc
     6361 gctgtcagcc agcagcttgc tccccgtgaa ggaatctctc aagaggcttt gcacacccag
     6421 atgctcactg cagtgcaaga aatttctcat ctcattgagc cgctggccag cgctgctcgg
     6481 gctgaagcct cccagctggg acacaaggtg tcccaaatgg cccagtactt tgaaccactc
     6541 accctggctg cagtgggtgc tgcgtctaag accctgagcc acccgcagca gatggcactt
     6601 ctggaccaga ctaaaacgtt ggcagagtct gccttgcagt tgctatatac tgccaaggag
     6661 gctggtggta accccaagca agcagcacac acccaggaag ccctggagga ggctgtgcag
     6721 atgatgacag aggccgtaga agacctgacg acgaccctca acgaagcagc cagtgctgca
     6781 ggagtcgttg gcggcatggt ggactctatc actcaggcca tcaaccagct agatgaagga
     6841 cctatgggtg acccagaagg ctcattcgta gattaccaga caaccatggt gaggacagcc
     6901 aaggccattg ctgtcactgt tcaggagatg gtaaccaagt caaacaccag ccctgaagag
     6961 ctaggccctc ttgccaacca gctgaccagt gactatggcc gactggcctc acaagccaag
     7021 cctgcagctg tggctgctga aaatgaagag ataggcgctc acatcaaaca ccgagtacag
     7081 gagctgggcc atggctgctc tgctctggtc accaaggcag gtgccttgca gtgtagcccc
     7141 agtgatgtct acaccaagaa ggagctcata gagtgtgccc gcagagtgtc agagaaggtc
     7201 tcccatgttc tggctgcact ccaggctggg aatcgtgaat tcaccggtat cctctggcat
     7261 gagatgtggc atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg
     7321 aaaggcatgt ttgaggtgct ggagcccttg catgctatga tggaacgggg cccccagact
     7381 ctgaaggaaa catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg
     7441 tgcaggaagt acatgaaatc agggaatgtc aaggacctca ctcaagcctg ggacctctat
     7501 tatcatgtgt tccgacgaat ctcaaagtaa gcggccgcga ctctagatca taatcagcca
     7561 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     7621 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     7681 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7741 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     7801 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     7861 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     7921 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     7981 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     8041 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8101 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8161 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8221 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8281 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8341 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     8401 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8461 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8521 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8581 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8641 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8701 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     8761 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     8821 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     8881 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     8941 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     9001 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     9061 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     9121 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     9181 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     9241 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     9301 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     9361 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     9421 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     9481 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     9541 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     9601 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     9661 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     9721 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     9781 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     9841 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     9901 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     9961 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
    10021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    10321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
    10861 gcattag
//
