LOCUS       sequence-402901-       10270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Talin(DeltaR1-10::FlnAIg1-8)-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-XT41-Cterminal-mEmerald
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6208..6217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6214..6216,6217..6219,6220..6933)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6259..6280)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6520..6539)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6867..6888
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7055..7176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7092..7111)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7146..7165
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7183..7638)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7320..7341)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7532..7551
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7665..7769
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7771..8128
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7771..7962
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7794..7814)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7979..8114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8163..8957
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8163..8957
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8217..8236)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8827..8846
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9145..9164)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9189..9236
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9565..10153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10054..10073
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgagctcaa gcttcgaatt ctagccacca tggttgcgct ttcgctgaag
      661 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      721 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      781 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      841 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcaaagaccc
      901 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      961 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
     1021 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
     1081 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1141 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1201 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccgc
     1261 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1321 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1381 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1441 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1501 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1561 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1621 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1681 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1741 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1801 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1861 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1921 aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1981 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     2041 atgccacctg cccagcagca gatcaccagt ggccagatgc accgacccaa actgaacccg
     2101 aagaaagccc gtgcctacgg gccaggcatc gagcccacag gcaacatggt gaagaagcgg
     2161 gcagagttca ctgtggaaac cagaagtgct ggccagggag aggtgctggt gtacgtggag
     2221 gacccggccg gacaccagga ggaggcaaaa gtgaccgcca ataacgacaa gaaccgcacc
     2281 ttctccgtct ggtacgtccc cgaggtgacg gggactcata aggttactgt gctctttgct
     2341 ggccagcaca tcgccaagag ccccttcgag gtgtacgtgg ataagtcaca gggtgacgcc
     2401 agcaaagtga cagcccaagg tcccggcctg gagcccagtg gcaacatcgc caacaagacc
     2461 acctactttg agatctttac ggcaggagct ggcacgggcg aggtcgaggt tgtgatccag
     2521 gaccccatgg gacagaaggg cacggtagag cctcagctgg aggcccgggg cgacagcaca
     2581 taccgctgca gctaccagcc caccatggag ggcgtccaca ccgtgcacgt cacgtttgcc
     2641 ggcgtgccca tccctcgcag cccctacact gtcactgttg gccaagcctg taacccgagt
     2701 gcctgccggg cggttggccg gggcctccag cccaagggtg tgcgggtgaa ggagacagct
     2761 gacttcaagg tgtacacaaa gggcgctggc agtggggagc tgaaggtcac cgtgaagggc
     2821 cccaagggag aggagcgcgt gaagcagaag gacctggggg atggcgtgta tggcttcgag
     2881 tattacccca tggtccctgg aacctatatc gtcaccatca cgtggggtgg tcagaacatc
     2941 gggcgcagtc ccttcgaagt gaaggtgggc accgagtgtg gcaatcagaa ggtacgggcc
     3001 tggggccctg ggctggaggg cggcgtcgtt ggcaagtcag cagactttgt ggtggaggct
     3061 atcggggacg acgtgggcac gctgggcttc tcggtggaag ggccatcgca ggctaagatc
     3121 gaatgtgacg acaagggcga cggctcctgt gatgtgcgct actggccgca ggaggctggc
     3181 gagtatgccg ttcacgtgct gtgcaacagc gaagacatcc gcctcagccc cttcatggct
     3241 gacatccgtg acgcgcccca ggacttccac ccagacaggg tgaaggcacg tgggcctgga
     3301 ttggagaaga caggtgtggc cgtcaacaag ccagcagagt tcacagtgga tgccaagcac
     3361 ggtggcaagg ccccacttcg ggtccaagtc caggacaatg aaggctgccc tgtggaggcg
     3421 ttggtcaagg acaacggcaa tggcacttac agctgctcct acgtgcccag gaagccggtg
     3481 aagcacacag ccatggtgtc ctggggaggc gtcagcatcc ccaacagccc cttcagggtg
     3541 aatgtgggag ctggcagcca ccccaacaag gtcaaagtat acggccccgg agtagccaag
     3601 acagggctca aggcccacga gcccacctac ttcactgtgg actgcgccga ggctggccag
     3661 ggggacgtca gcatcggcat caagtgtgcc cctggagtgg taggccccgc cgaagctgac
     3721 atcgacttcg acatcatccg caatgacaat gacaccttca cggtcaagta cacgccccgg
     3781 ggggctggca gctacaccat tatggtcctc tttgctgacc aggccacgcc caccagcccc
     3841 atccgagtca aggtggagcc ctctcatgac gccagtaagg tgaaggccga gggccctggc
     3901 ctcagtcgca ctggtgtcga gcttggcaag cccacccact tcacagtaaa tgccaaagct
     3961 gctggcaaag gcaagctgga cgtccagttc tcaggactca ccaaggggga tgcagtgcga
     4021 gatgtggaca tcatcgacca ccatgacaac acctacacag tcaagtacac gcctgtccag
     4081 cagggtccag taggcgtcaa tgtcacttat ggaggggatc ccatccctaa gagccctttc
     4141 tcagtggcag tatctccaag cctggacctc agcaagatca aggtgtctgg cctgggagag
     4201 aaggtggacg ttggcaaaga ccaggagttc acagtcaaat caaagggtgc tggtggtcaa
     4261 ggcaaagtgg catccaagat tgtgggcccc tcgggtgcag cggtgccctg caaggtggag
     4321 ccaggcctgg gggctgacaa cagtgtggtg cgcttcctgc cccgtgagga agggccctat
     4381 gaggtggagg tgacctatga cggcgtgccc gtgcctggca gcccctttcc tctggaagct
     4441 gtgggtaccc aggcctgcat tacagcagcc agtgctgtgt ctggtatcat tgctgacctc
     4501 gacaccacca tcatgttcgc tactgctggc acacttaacc gtgagggtgc tgaaactttt
     4561 gctgaccacc gggagggtat cttaaagaca gcaaaggttc ttgtggagga caccaaggtc
     4621 ctagtgcaga atgcagctgg gagccaggag aagttggcac aagccgccca gtcctccgtg
     4681 gccaccatta cccgcctcgc tgatgtggtc aagctcggtg cagccagcct aggagccgaa
     4741 gaccctgaaa ctcaggtggt gctgatcaat gcagtaaagg acgtagccaa ggccctgggt
     4801 gacctcatca gcgctacgaa ggctgcagcg ggcaaagttg gggatgaccc tgcagtgtgg
     4861 cagctcaaga actctgccaa ggtgatggtg accaatgtga catcattgct caagacagtg
     4921 aaggctgtgg aagatgaggc caccaaaggc acacgggccc tagaggcaac cacagagcac
     4981 atacgtcagg aactggcggt cttctgttcc ccagagccac ctgccaagac ctctacccct
     5041 gaagacttca tccgaatgac caagggtatt actatggcaa cagccaaagc cgttgctgct
     5101 ggcaattcct gtcgacagga agatgtcatt gccacagcca atctgagccg acgggctatt
     5161 gcggacatgc ttcgggcttg caaggaagca gctttccacc cagaagtggc gcctgatgta
     5221 cggctccgag ccctgcacta tggccgggag tgtgccaatg gttacctgga actgctggac
     5281 cacgtgctgc tgacccttca gaagccaaac ccagacctga agcagcagct aactggacac
     5341 tcaaagcggg ttgctggctc tgtgactgag ctcatccagg ctgctgaagc catgaaagga
     5401 acagagtggg tggacccaga ggaccctact gtcattgctg agaatgagct cttgggagcc
     5461 gcagccgcca tcgaggctgc agccaagaag ctggagcagc tgaagcccag ggccaaaccc
     5521 aaggaggcgg atgagtcctt gaactttgag gaacaaatcc tagaagctgc caagtccatc
     5581 gctgcagcca ccagtgcact ggtaaaggct gcgtcagcag cccagaggga gctggtggct
     5641 caaggaaagg tgggcgccat tccagccaat gcactggatg atgggcagtg gtcgcagggc
     5701 ctcatttccg ctgcccgtat ggtggctgcg gccaccaaca atctgtgtga agcagctaac
     5761 gcagctgtcc agggccatgc tagccaggag aaactcattt cctcagccaa gcaagtagct
     5821 gcctccacag ctcagctcct ggtagcttgc aaggtcaaag cagatcagga ctctgaggca
     5881 atgaaacggc tccaggctgc tggcaatgca gtgaagaggg cctcagataa cctggtaaag
     5941 gcggcccaga aagctgcagc cttcgaagac caggagaatg agacggtggt ggtgaaggag
     6001 aaaatggttg ggggcattgc ccagattatc gcagcacagg aagagatgct tcggaaggaa
     6061 cgagagctgg aagaggctcg gaaaaagctc gcccagatcc ggcagcagca gtacaagttc
     6121 ttgccttcag agcttcgaga cgagcacggc gctgggtccg ggtccgggca agggtccggc
     6181 gcaggctccg cgtcccgatc accggtcgcc accatggtga gcaagggcga ggagctgttc
     6241 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6301 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     6361 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttgac ctacggcgtg
     6421 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     6481 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     6541 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     6601 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     6661 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagacccgc
     6721 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     6781 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc
     6841 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     6901 atcactctcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     6961 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     7021 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     7081 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7141 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     7201 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     7261 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     7321 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     7381 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     7441 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     7501 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     7561 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     7621 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7681 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7741 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     7801 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7861 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7921 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7981 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8041 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8101 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     8161 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     8221 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     8281 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     8341 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     8401 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     8461 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     8521 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     8581 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     8641 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     8701 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     8761 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     8821 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8881 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8941 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     9001 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9061 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9121 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     9181 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     9241 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccaagac cccattgggg
     9301 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     9361 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     9421 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9541 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9601 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9661 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9721 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9781 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9841 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9901 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9961 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10021 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10081 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10141 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10201 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10261 catgcattag
//
