LOCUS       sequence-402903-       10597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Talin(DeltaR4-12::FlnAIg1-8)-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-XT46-Cterminal-mEmerald
FEATURES             Location/Qualifiers
     source          1..10597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6535..6544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6541..6543,6544..6546,6547..7260)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6586..6607)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6847..6866)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7194..7215
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7382..7503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7419..7438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7473..7492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7510..7965)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7647..7668)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7859..7878
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7992..8096
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8098..8455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8098..8289
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8121..8141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8306..8441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8490..9284
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8490..9284
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8544..8563)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9154..9173
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9472..9491)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9516..9563
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9892..10480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10381..10400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgagctcaa gcttcgaatt ctagccacca tggttgcgct ttcgctgaag
      661 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      721 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      781 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      841 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcaaagaccc
      901 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      961 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
     1021 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
     1081 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1141 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1201 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccgc
     1261 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1321 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1381 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1441 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1501 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1561 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1621 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1681 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1741 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1801 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1861 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1921 aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1981 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     2041 atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggaca catgccacct
     2101 ctgacttcag cccagcaggc gctcactgga accattaact ccagcatgca ggctgtgcag
     2161 gctgcccagg ccactctgga tgactttgag actctacccc ctcttggcca ggatgctgcc
     2221 tccaaagctt ggcgtaagaa caagatggat gaatcaaagc atgagatcca ctcccaggta
     2281 gatgccatca cagctggcac tgcctctgtg gtaaacctga cagcaggaga tcctgcagag
     2341 acagactata cagcagtggg ctgtgcagtc accaccatct cttccaacct gacggagatg
     2401 tcccgtgggg tgaagctatt ggctgccctg ctggaggatg aaggcggcaa tggccggccc
     2461 cttctgcaag cagcaaaggg ccttgcaggg gctgtgtcgg aactgcttcg cagtgctcag
     2521 cccgccagcg ctgagcctcg tcagaacctg ctgcaggcag ccgggaacgt gggccaggcc
     2581 agtggggagc tgttgcagca aattggggaa agtgacactg acccccactt ccaggatgtt
     2641 ctaatgcagc tagcaaacgc agtggcaagt gctgcagccg ccctggtcct caaggccaag
     2701 agtgtggccc agcgcacaga ggattccggg cttcagaccc aagtgattgc tgcagctacg
     2761 caatgtgctc tgtccacttc ccagctggtg gcctgtacca aggtggtagc acctacaatc
     2821 agctcacctg tgtgccaaga acagctagtc gaggctgggc gactggtggc caaagctgtg
     2881 gagggctgtg tgtctgcctc gcaagcagct acagaagatg gacagctgct tcgaggggta
     2941 ggagcagcag ccacggctgt cacccaggcc ctcaatgagc tgctgcagca cgtgaaggcc
     3001 cacgccaccg gagctgggcc tgccggtcgg tatgaccaag ccactgatac catcctcacc
     3061 gtcactgaaa acatcttcag ctccatgggt gatgctgggg agatggtgcg ccaggcccgc
     3121 atcctggccc aagccacctc agacctggtc aatgctatca aggccgatgc tgagggggag
     3181 agtgatctgg agaactctag aaagctcctg agtgctgcca agatcctcgc tgatgccacc
     3241 gccaagatgg tggaggcggc caagggagca gccgcccacc ctgacagtga ggaacagcag
     3301 cagcgactgc gtgaagcagc tgaggggctt cgcatggcca ccaatgcagc tgcgcagaac
     3361 gccatcaaga agcccaaact gaacccgaag aaagcccgtg cctacgggcc aggcatcgag
     3421 cccacaggca acatggtgaa gaagcgggca gagttcactg tggaaaccag aagtgctggc
     3481 cagggagagg tgctggtgta cgtggaggac ccggccggac accaggagga ggcaaaagtg
     3541 accgccaata acgacaagaa ccgcaccttc tccgtctggt acgtccccga ggtgacgggg
     3601 actcataagg ttactgtgct ctttgctggc cagcacatcg ccaagagccc cttcgaggtg
     3661 tacgtggata agtcacaggg tgacgccagc aaagtgacag cccaaggtcc cggcctggag
     3721 cccagtggca acatcgccaa caagaccacc tactttgaga tctttacggc aggagctggc
     3781 acgggcgagg tcgaggttgt gatccaggac cccatgggac agaagggcac ggtagagcct
     3841 cagctggagg cccggggcga cagcacatac cgctgcagct accagcccac catggagggc
     3901 gtccacaccg tgcacgtcac gtttgccggc gtgcccatcc ctcgcagccc ctacactgtc
     3961 actgttggcc aagcctgtaa cccgagtgcc tgccgggcgg ttggccgggg cctccagccc
     4021 aagggtgtgc gggtgaagga gacagctgac ttcaaggtgt acacaaaggg cgctggcagt
     4081 ggggagctga aggtcaccgt gaagggcccc aagggagagg agcgcgtgaa gcagaaggac
     4141 ctgggggatg gcgtgtatgg cttcgagtat taccccatgg tccctggaac ctatatcgtc
     4201 accatcacgt ggggtggtca gaacatcggg cgcagtccct tcgaagtgaa ggtgggcacc
     4261 gagtgtggca atcagaaggt acgggcctgg ggccctgggc tggagggcgg cgtcgttggc
     4321 aagtcagcag actttgtggt ggaggctatc ggggacgacg tgggcacgct gggcttctcg
     4381 gtggaagggc catcgcaggc taagatcgaa tgtgacgaca agggcgacgg ctcctgtgat
     4441 gtgcgctact ggccgcagga ggctggcgag tatgccgttc acgtgctgtg caacagcgaa
     4501 gacatccgcc tcagcccctt catggctgac atccgtgacg cgccccagga cttccaccca
     4561 gacagggtga aggcacgtgg gcctggattg gagaagacag gtgtggccgt caacaagcca
     4621 gcagagttca cagtggatgc caagcacggt ggcaaggccc cacttcgggt ccaagtccag
     4681 gacaatgaag gctgccctgt ggaggcgttg gtcaaggaca acggcaatgg cacttacagc
     4741 tgctcctacg tgcccaggaa gccggtgaag cacacagcca tggtgtcctg gggaggcgtc
     4801 agcatcccca acagcccctt cagggtgaat gtgggagctg gcagccaccc caacaaggtc
     4861 aaagtatacg gccccggagt agccaagaca gggctcaagg cccacgagcc cacctacttc
     4921 actgtggact gcgccgaggc tggccagggg gacgtcagca tcggcatcaa gtgtgcccct
     4981 ggagtggtag gccccgccga agctgacatc gacttcgaca tcatccgcaa tgacaatgac
     5041 accttcacgg tcaagtacac gccccggggg gctggcagct acaccattat ggtcctcttt
     5101 gctgaccagg ccacgcccac cagccccatc cgagtcaagg tggagccctc tcatgacgcc
     5161 agtaaggtga aggccgaggg ccctggcctc agtcgcactg gtgtcgagct tggcaagccc
     5221 acccacttca cagtaaatgc caaagctgct ggcaaaggca agctggacgt ccagttctca
     5281 ggactcacca agggggatgc agtgcgagat gtggacatca tcgaccacca tgacaacacc
     5341 tacacagtca agtacacgcc tgtccagcag ggtccagtag gcgtcaatgt cacttatgga
     5401 ggggatccca tccctaagag ccctttctca gtggcagtat ctccaagcct ggacctcagc
     5461 aagatcaagg tgtctggcct gggagagaag gtggacgttg gcaaagacca ggagttcaca
     5521 gtcaaatcaa agggtgctgg tggtcaaggc aaagtggcat ccaagattgt gggcccctcg
     5581 ggtgcagcgg tgccctgcaa ggtggagcca ggcctggggg ctgacaacag tgtggtgcgc
     5641 ttcctgcccc gtgaggaagg gccctatgag gtggaggtga cctatgacgg cgtgcccgtg
     5701 cctggcagcc cctttcctct ggaagctgtg gagtgggtgg acccagagga ccctactgtc
     5761 attgctgaga atgagctctt gggagccgca gccgccatcg aggctgcagc caagaagctg
     5821 gagcagctga agcccagggc caaacccaag gaggcggatg agtccttgaa ctttgaggaa
     5881 caaatcctag aagctgccaa gtccatcgct gcagccacca gtgcactggt aaaggctgcg
     5941 tcagcagccc agagggagct ggtggctcaa ggaaaggtgg gcgccattcc agccaatgca
     6001 ctggatgatg ggcagtggtc gcagggcctc atttccgctg cccgtatggt ggctgcggcc
     6061 accaacaatc tgtgtgaagc agctaacgca gctgtccagg gccatgctag ccaggagaaa
     6121 ctcatttcct cagccaagca agtagctgcc tccacagctc agctcctggt agcttgcaag
     6181 gtcaaagcag atcaggactc tgaggcaatg aaacggctcc aggctgctgg caatgcagtg
     6241 aagagggcct cagataacct ggtaaaggcg gcccagaaag ctgcagcctt cgaagaccag
     6301 gagaatgaga cggtggtggt gaaggagaaa atggttgggg gcattgccca gattatcgca
     6361 gcacaggaag agatgcttcg gaaggaacga gagctggaag aggctcggaa aaagctcgcc
     6421 cagatccggc agcagcagta caagttcttg ccttcagagc ttcgagacga gcacggcgct
     6481 gggtccgggt ccgggcaagg gtccggcgca ggctccgcgt cccgatcacc ggtcgccacc
     6541 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     6601 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     6661 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     6721 ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     6781 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     6841 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     6901 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     6961 aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac
     7021 ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc
     7081 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     7141 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     7201 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     7261 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7321 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7381 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7501 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7561 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7621 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7681 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7741 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7801 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     7861 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7921 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     7981 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8041 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     8101 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8161 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8221 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8281 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8341 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8401 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     8461 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     8521 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     8581 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     8641 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     8701 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8761 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8821 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8881 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8941 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     9001 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     9061 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     9121 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     9181 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     9241 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     9301 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     9361 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9421 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     9481 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     9541 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     9601 cgatacccca ccaagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     9661 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     9721 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     9781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10561 tgattctgtg gataaccgta ttaccgccat gcattag
//
