LOCUS       sequence-402904-        7370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP-Talin(DeltaR1-12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-T29-Nterminal-EGFP
FEATURES             Location/Qualifiers
     source          1..7370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      604..613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(610..612,613..615,616..1326)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(610..612,613..615,616..1326)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(655..676)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(916..935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1263..1284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4155..4276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4192..4211)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4246..4265
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4283..4738)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4420..4441)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4632..4651
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4765..4869
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4871..5228
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4871..5062
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4894..4914)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5079..5214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5263..6057
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5263..6057
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5317..5336)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5927..5946
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6245..6264)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6289..6336
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6665..7253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7154..7173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      781 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1021 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1081 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1201 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1261 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1321 tacaagtccg gactcagatc tcgagctcaa gctagcttcg aattcgcaga agctgccacc
     1381 atggttgcgc tttcgctgaa gattagcatt gggaatgtgg tgaagacgat gcaatttgag
     1441 ccatctacca tggtgtacga cgcctgccgc atgattcgtg agcggatccc agaggccctg
     1501 gctggccctc ccaacgactt tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc
     1561 tggctagagg ctgggaaagc tttggactac tacatgctcc gaaatgggga caccatggag
     1621 tacagaaaaa agcagagacc cctgaagata cggatgttag acggaacggt gaagactatc
     1681 atggtggatg actccaagac cgtcacagac atgctcatga ccatttgtgc ccgaattggt
     1741 atcaccaacc atgatgagta ttcactggtt cgagagctga tggaagaaaa gaaagatgag
     1801 gggacaggga ccctgagaaa agacaagacc ctactgcgag atgaaaagaa gatggagaag
     1861 ctaaagcaga agttgcacac agacgatgag ttgaactggc tggaccatgg gcggacactg
     1921 agggaacagg gagtggaaga gcatgagacg ttgctgctgc ggagaaagtt cttctactca
     1981 gaccagaatg tggattcccg agaccctgta cagctgaacc ttctctatgt gcaggcacga
     2041 gatgacatcc tgaatggctc ccatcctgtc tcctttgaca aggcctgtga atttgcaggc
     2101 ttccagtgcc agatccagtt tggacctcac aatgaacaga agcacaaggc tggcttcctt
     2161 gacctgaagg acttcctgcc caaggagtat gtgaagcaga agggagagcg taagatcttt
     2221 caggcacaca agaattgtgg gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag
     2281 ctggcccgtt ccctcaagac ttacggtgtc tccttcttcc tagtcaagga aaagatgaag
     2341 gggaagaata aactggtgcc caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg
     2401 gatgagaaga ccaaggaggt gattcaggaa tggagcctca ccaacatcaa acgctgggct
     2461 gcctctccca agagctttac tctggacttt ggagactatc aggatggcta ctactcagta
     2521 cagacgacag aaggcgagca gatcgcacag ctcattgctg gctacataga tatcatcctt
     2581 aagaagaaaa aaagcaagga ccattttggg ctggagggag atgaagagtc tactatgttg
     2641 gaggactcag tgtctcccaa aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg
     2701 aaagtggagc acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct
     2761 gagaatttcc aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg
     2821 cacgagtggg tggacccaga ggaccctact gtcattgctg agaatgagct cttgggagcc
     2881 gcagccgcca tcgaggctgc agccaagaag ctggagcagc tgaagcccag ggccaaaccc
     2941 aaggaggcgg atgagtcctt gaactttgag gaacaaatcc tagaagctgc caagtccatc
     3001 gctgcagcca ccagtgcact ggtaaaggct gcgtcagcag cccagaggga gctggtggct
     3061 caaggaaagg tgggcgccat tccagccaat gcactggatg atgggcagtg gtcgcagggc
     3121 ctcatttccg ctgcccgtat ggtggctgcg gccaccaaca atctgtgtga agcagctaac
     3181 gcagctgtcc agggccatgc tagccaggag aaactcattt cctcagccaa gcaagtagct
     3241 gcctccacag ctcagctcct ggtagcttgc aaggtcaaag cagatcagga ctctgaggca
     3301 atgaaacggc tccaggctgc tggcaatgca gtgaagaggg cctcagataa cctggtaaag
     3361 gcggcccaga aagctgcagc cttcgaagac caggagaatg agacggtggt ggtgaaggag
     3421 aaaatggttg ggggcattgc ccagattatc gcagcacagg aagagatgct tcggaaggaa
     3481 cgagagctgg aagaggctcg gaaaaagctc gcccagatcc ggcagcagca gtacaagttc
     3541 ttgccttcag agcttcgaga cgagcactaa aaaaccggtg tatttaatgc agacccagcc
     3601 cagagactgt gcctgccact accaaagcct tctgggctgc tcaggaccca agctgcccaa
     3661 ccccagcact cccccaaagt gcctgccaaa ccctgggcct ggccctgccc agtcccactg
     3721 caagccgtgt cctctccctg actcccaagt gcctttgcac cctagggccc ctaagtgcct
     3781 gcccccctcc agagtattaa cgctccaaga gtattattaa tgctgccgta cctcagtttg
     3841 aacctgccag ggccccagct gctccagcct gccagcagct tccagccagt ccccacggcc
     3901 acgtcagctc aactcatccc tttttgatac tatatcccct acccagctac ctatggggct
     3961 tgagggttgt aaacccaaac aggtcagact cccgggattc tgcagtcgac ggtaccgcgg
     4021 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     4081 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4141 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4201 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4261 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     4321 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4381 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4441 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4501 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     4561 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4621 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4681 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     4741 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4861 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     4921 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4981 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5041 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5221 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5281 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5341 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5401 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     5461 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5521 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5581 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5641 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5701 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5761 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     5821 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     5881 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     5941 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6001 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6061 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6121 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6181 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     6241 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     6301 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     6361 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     6421 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     6481 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     6541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6781 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7321 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag
//
