LOCUS       sequence-402908-       12349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Talin(FullLength)-L432G-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-T100-L432G-Cterminal-mEmerald
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8287..8296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8293..8295,8296..8298,8299..9012)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8338..8359)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8599..8618)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8946..8967
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9134..9255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9171..9190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9225..9244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9262..9717)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9399..9420)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9611..9630
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9744..9848
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9850..10207
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9850..10041
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9873..9893)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10058..10193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10242..11036
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10242..11036
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10296..10315)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10906..10925
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11224..11243)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11268..11315
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11644..12232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12133..12152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgagctcaa gcttcgaatt ctagccacca tggttgcgct ttcgctgaag
      661 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      721 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      781 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      841 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcagagaccc
      901 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      961 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
     1021 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
     1081 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1141 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1201 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccga
     1261 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1321 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1381 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1441 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1501 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1561 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1621 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1681 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1741 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1801 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1861 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1921 aagtcaacag tcggtcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1981 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     2041 atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggaca catgccacct
     2101 ctgacttcag cccagcaggc gctcactgga accattaact ccagcatgca ggctgtgcag
     2161 gctgcccagg ccactctgga tgactttgag actctacccc ctcttggcca ggatgctgcc
     2221 tccaaagctt ggcgtaagaa caagatggat gaatcaaagc atgagatcca ctcccaggta
     2281 gatgccatca cagctggcac tgcctctgtg gtaaacctga cagcaggaga tcctgcagag
     2341 acagactata cagcagtggg ctgtgcagtc accaccatct cttccaacct gacggagatg
     2401 tcccgtgggg tgaagctatt ggctgccctg ctggaggatg aaggcggcaa tggccggccc
     2461 cttctgcaag cagcaaaggg ccttgcaggg gctgtgtcgg aactgcttcg cagtgctcag
     2521 cccgccagcg ctgagcctcg tcagaacctg ctgcaggcag ccgggaacgt gggccaggcc
     2581 agtggggagc tgttgcagca aattggggaa agtgacactg acccccactt ccaggatgtt
     2641 ctaatgcagc tagcaaacgc agtggcaagt gctgcagccg ccctggtcct caaggccaag
     2701 agtgtggccc agcgcacaga ggattccggg cttcagaccc aagtgattgc tgcagctacg
     2761 caatgtgctc tgtccacttc ccagctggtg gcctgtacca aggtggtagc acctacaatc
     2821 agctcacctg tgtgccaaga acagctagtc gaggctgggc gactggtggc caaagctgtg
     2881 gagggctgtg tgtctgcctc gcaagcagct acagaagatg gacagctgct tcgaggggta
     2941 ggagcagcag ccacggctgt cacccaggcc ctcaatgagc tgctgcagca cgtgaaggcc
     3001 cacgccaccg gagctgggcc tgccggtcgg tatgaccaag ccactgatac catcctcacc
     3061 gtcactgaaa acatcttcag ctccatgggt gatgctgggg agatggtgcg ccaggcccgc
     3121 atcctggccc aagccacctc agacctggtc aatgctatca aggccgatgc tgagggggag
     3181 agtgatctgg agaactctag aaagctcctg agtgctgcca agatcctcgc tgatgccacc
     3241 gccaagatgg tggaggcggc caagggagca gccgcccacc ctgacagtga ggaacagcag
     3301 cagcgactgc gtgaagcagc tgaggggctt cgcatggcca ccaatgcagc tgcgcagaac
     3361 gccatcaaga agaagttggt gcagcgcctg gagcatgcag ccaagcaagc tgcagcctct
     3421 gcaacacaga ccattgctgc agcccaacat gcagcctctg cccccaaggc ctctgcgggc
     3481 ccccagcccc tactggtgca gagctgtaag gccgtggcag agcagattcc gttgctggtg
     3541 cagggtgtcc gaggaagtca agctcagcct gacagcccta gtgctcagct cgccctcatt
     3601 gctgccagcc agagcttcct gcagccaggt gggaagatgg tggcagcggc aaaagcctca
     3661 gtgccaacaa ttcaggacca ggcttcagcg atgcagctga gtcagtgcgc aaagaaccta
     3721 ggcactgccc tggccgagct gcgcactgct gctcagaagg ctcaggaggc atgtggacct
     3781 ttggagatgg attctgcact gagtgttgta caaaatctag agaaagacct acaggaaata
     3841 aaagcagcag ctcgagatgg caagcttaag cccttacctg gggaaacaat ggagaagtgt
     3901 acccaagatc ttggcaacag caccaaagcg gtgagctctg ccatcgccaa gctgctgggg
     3961 gagattgccc agggcaacga gaactatgca ggtattgcag ctcgggatgt agctggtgga
     4021 ctaaggtcac tagcccaggc tgcgcggggt gtggctgcgc tgacatcaga tcctgcagtg
     4081 caggccattg tgcttgacac agccagcgac gttctggaca aggccagcag cctcattgaa
     4141 gaggcaaaaa aagcatctgg acacccaggg gacccagaaa gccagcaaag gcttgctcag
     4201 gtagctaaag cagtgaccca agccctgaac cgctgtgtca gctgcctgcc tggccaaaga
     4261 gacgtggata atgccctacg agccgttgga gatgccagca agcgactcct gagtgacttg
     4321 cttcctccaa gcacggggac atttcaagaa gcacagagcc gattgaatga agctgcagct
     4381 gggttaaatc aggccgccac agaactggtg caagcctccc gaggaactcc tcaggacctg
     4441 gctcgggcct ccggccgatt tggacaggac ttcagcacct tcctggaagc tggtgtggag
     4501 atggccggac aggccccgag ccaggaggac cgagcccagg tggtgtccaa cctgaagggc
     4561 atatccatgt cttcaagcaa acttctcctt gccgctaagg ccttgtccac agaccctgct
     4621 tctcccaacc tcaagagtca gctggctgcg gctgcccggg cagtgacgga cagcatcaac
     4681 cagctcatca ccatgtgcac ccagcaggca cctggccaga aggagtgtga caatgcactt
     4741 cggcagctag agacagtccg agaactcctg gagaatccag tccagcccat caacgacatg
     4801 tcctacttcg gttgcttgga cagtgtcatg gagaactcca aggtcctagg tgaggccatg
     4861 actggcatct cccaaaatgc caagaatgga aatctgccgg agtttggaga tgccattgcc
     4921 acagcctcca aggctctctg tggcttcacg gaggcagccg cacaggcagc atatctagtt
     4981 ggtgtctctg accccaacag ccaagctgga cagcaaggac tggtggaacc cacacagttt
     5041 gcccgtgcaa accaggcaat tcagatggcc tgtcagagtc tgggggagcc tggctgtacc
     5101 caggcccagg tgttatctgc agccactatt gtagccaaac acacatctgc attgtgtaac
     5161 agctgtcgcc tggcttccgc taggactgcc aatcccactg ccaagcgcca gtttgtacag
     5221 tcagccaagg aggtggccaa cagtacagcc aatcttgtca agaccatcaa ggcactagat
     5281 ggggacttca cagaagagaa ccgtgcccag tgccgagcag ccacagcccc tctgctggaa
     5341 gctgtggata acctgagtgc ctttgcctcc aaccctgagt tctccagcgt ccctgcccag
     5401 atcagccctg agggcagagc agccatggag cccattgtaa tctctgctaa gacaatgttg
     5461 gagagtgctg gagggctcat ccagacagct cgggcgttag cagtcaatcc ccgagacccc
     5521 ccacgttggt ctgtgctagc tggccactct cggactgtct cagattccat caagaaactt
     5581 attacaagca tgagggacaa agccccaggg cagctggagt gtgagacagc cattgcggct
     5641 ctgaacagct gcttgcggga cctagatcag gcttcgcttg ccgctgtcag ccagcagctt
     5701 gctccccgtg aaggaatctc tcaagaggct ttgcacaccc agatgctcac tgcagtgcaa
     5761 gaaatttctc atctcattga gccgctggcc agcgctgctc gggctgaagc ctcccagctg
     5821 ggacacaagg tgtcccaaat ggcccagtac tttgaaccac tcaccctggc tgcagtgggt
     5881 gctgcgtcta agaccctgag ccacccgcag cagatggcac ttctggacca gactaaaacg
     5941 ttggcagagt ctgccttgca gttgctatat actgccaagg aggctggtgg taaccccaag
     6001 caagcagcac acacccagga agccctggag gaggctgtgc agatgatgac agaggccgta
     6061 gaagacctga cgacgaccct caacgaagca gccagtgctg caggagtcgt tggcggcatg
     6121 gtggactcta tcactcaggc catcaaccag ctagatgaag gacctatggg tgacccagaa
     6181 ggctcattcg tagattacca gacaaccatg gtgaggacag ccaaggccat tgctgtcact
     6241 gttcaggaga tggtaaccaa gtcaaacacc agccctgaag agctaggccc tcttgccaac
     6301 cagctgacca gtgactatgg ccgactggcc tcacaagcca agcctgcagc tgtggctgct
     6361 gaaaatgaag agataggcgc tcacatcaaa caccgagtac aggagctggg ccatggctgc
     6421 tctgctctgg tcaccaaggc aggtgccttg cagtgtagcc ccagtgatgt ctacaccaag
     6481 aaggagctca tagagtgtgc ccgcagagtg tcagagaagg tctcccatgt tctggctgca
     6541 ctccaggctg ggaatcgtgg tacccaggcc tgcattacag cagccagtgc tgtgtctggt
     6601 atcattgctg acctcgacac caccatcatg ttcgctactg ctggcacact taaccgtgag
     6661 ggtgctgaaa cttttgctga ccaccgggag ggtatcttaa agacagcaaa ggttcttgtg
     6721 gaggacacca aggtcctagt gcagaatgca gctgggagcc aggagaagtt ggcacaagcc
     6781 gcccagtcct ccgtggccac cattacccgc ctcgctgatg tggtcaagct cggtgcagcc
     6841 agcctaggag ccgaagaccc tgaaactcag gtggtgctga tcaatgcagt aaaggacgta
     6901 gccaaggccc tgggtgacct catcagcgct acgaaggctg cagcgggcaa agttggggat
     6961 gaccctgcag tgtggcagct caagaactct gccaaggtga tggtgaccaa tgtgacatca
     7021 ttgctcaaga cagtgaaggc tgtggaagat gaggccacca aaggcacacg ggccctagag
     7081 gcaaccacag agcacatacg tcaggaactg gcggtcttct gttccccaga gccacctgcc
     7141 aagacctcta cccctgaaga cttcatccga atgaccaagg gtattactat ggcaacagcc
     7201 aaagccgttg ctgctggcaa ttcctgtcga caggaagatg tcattgccac agccaatctg
     7261 agccgacggg ctattgcgga catgcttcgg gcttgcaagg aagcagcttt ccacccagaa
     7321 gtggcgcctg atgtacggct ccgagccctg cactatggcc gggagtgtgc caatggttac
     7381 ctggaactgc tggaccacgt gctgctgacc cttcagaagc caaacccaga cctgaagcag
     7441 cagctaactg gacactcaaa gcgggttgct ggctctgtga ctgagctcat ccaggctgct
     7501 gaagccatga aaggaacaga gtgggtggac ccagaggacc ctactgtcat tgctgagaat
     7561 gagctcttgg gagccgcagc cgccatcgag gctgcagcca agaagctgga gcagctgaag
     7621 cccagggcca aacccaagga ggcggatgag tccttgaact ttgaggaaca aatcctagaa
     7681 gctgccaagt ccatcgctgc agccaccagt gcactggtaa aggctgcgtc agcagcccag
     7741 agggagctgg tggctcaagg aaaggtgggc gccattccag ccaatgcact ggatgatggg
     7801 cagtggtcgc agggcctcat ttccgctgcc cgtatggtgg ctgcggccac caacaatctg
     7861 tgtgaagcag ctaacgcagc tgtccagggc catgctagcc aggagaaact catttcctca
     7921 gccaagcaag tagctgcctc cacagctcag ctcctggtag cttgcaaggt caaagcagat
     7981 caggactctg aggcaatgaa acggctccag gctgctggca atgcagtgaa gagggcctca
     8041 gataacctgg taaaggcggc ccagaaagct gcagccttcg aagaccagga gaatgagacg
     8101 gtggtggtga aggagaaaat ggttgggggc attgcccaga ttatcgcagc acaggaagag
     8161 atgcttcgga aggaacgaga gctggaagag gctcggaaaa agctcgccca gatccggcag
     8221 cagcagtaca agttcttgcc ttcagagctt cgagacgagc acggctccgc gtcccgatca
     8281 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     8341 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     8401 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     8461 ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc
     8521 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     8581 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     8641 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     8701 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     8761 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     8821 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     8881 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     8941 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     9001 ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     9061 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     9121 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9181 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9241 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9301 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9361 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9421 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9481 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9541 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9601 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     9661 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     9721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9841 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     9901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9961 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10021 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10081 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10141 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    10201 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
    10261 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    10321 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
    10381 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
    10441 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    10501 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    10561 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    10621 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    10681 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    10741 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
    10801 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    10861 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    10921 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    10981 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    11041 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    11101 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    11161 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
    11221 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
    11281 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
    11341 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
    11401 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
    11461 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
    11521 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    11581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    11641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    11701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    11761 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    11821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    11881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    12001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    12061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    12121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    12241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    12301 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattag
//
