LOCUS       sequence-402911-        8333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP-Talin(DeltaR1-10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-T41-Nterminal-EGFP
FEATURES             Location/Qualifiers
     source          1..8333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      604..613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(610..612,613..615,616..1326)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(610..612,613..615,616..1326)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(655..676)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(916..935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1263..1284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5118..5239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5155..5174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5209..5228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5246..5701)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5383..5404)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5595..5614
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5728..5832
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5834..6191
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5834..6025
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5857..5877)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6042..6177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6226..7020
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6226..7020
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6280..6299)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6890..6909
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7208..7227)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7252..7299
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7628..8216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8117..8136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      781 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1021 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1081 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1201 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1261 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1321 tacaagtccg gactcagatc tcgagctcaa gctagcttcg aattcgcaga agctgccacc
     1381 atggttgcgc tttcgctgaa gattagcatt gggaatgtgg tgaagacgat gcaatttgag
     1441 ccatctacca tggtgtacga cgcctgccgc atgattcgtg agcggatccc agaggccctg
     1501 gctggccctc ccaacgactt tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc
     1561 tggctagagg ctgggaaagc tttggactac tacatgctcc gaaatgggga caccatggag
     1621 tacagaaaaa agcagagacc cctgaagata cggatgttag acggaacggt gaagactatc
     1681 atggtggatg actccaagac cgtcacagac atgctcatga ccatttgtgc ccgaattggt
     1741 atcaccaacc atgatgagta ttcactggtt cgagagctga tggaagaaaa gaaagatgag
     1801 gggacaggga ccctgagaaa agacaagacc ctactgcgag atgaaaagaa gatggagaag
     1861 ctaaagcaga agttgcacac agacgatgag ttgaactggc tggaccatgg gcggacactg
     1921 agggaacagg gagtggaaga gcatgagacg ttgctgctgc ggagaaagtt cttctactca
     1981 gaccagaatg tggattcccg agaccctgta cagctgaacc ttctctatgt gcaggcacga
     2041 gatgacatcc tgaatggctc ccatcctgtc tcctttgaca aggcctgtga atttgcaggc
     2101 ttccagtgcc agatccagtt tggacctcac aatgaacaga agcacaaggc tggcttcctt
     2161 gacctgaagg acttcctgcc caaggagtat gtgaagcaga agggagagcg taagatcttt
     2221 caggcacaca agaattgtgg gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag
     2281 ctggcccgtt ccctcaagac ttacggtgtc tccttcttcc tagtcaagga aaagatgaag
     2341 gggaagaata aactggtgcc caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg
     2401 gatgagaaga ccaaggaggt gattcaggaa tggagcctca ccaacatcaa acgctgggct
     2461 gcctctccca agagctttac tctggacttt ggagactatc aggatggcta ctactcagta
     2521 cagacgacag aaggcgagca gatcgcacag ctcattgctg gctacataga tatcatcctt
     2581 aagaagaaaa aaagcaagga ccattttggg ctggagggag atgaagagtc tactatgttg
     2641 gaggactcag tgtctcccaa aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg
     2701 aaagtggagc acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct
     2761 gagaatttcc aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg
     2821 caccgaggta cccaggcctg cattacagca gccagtgctg tgtctggtat cattgctgac
     2881 ctcgacacca ccatcatgtt cgctactgct ggcacactta accgtgaggg tgctgaaact
     2941 tttgctgacc accgggaggg tatcttaaag acagcaaagg ttcttgtgga ggacaccaag
     3001 gtcctagtgc agaatgcagc tgggagccag gagaagttgg cacaagccgc ccagtcctcc
     3061 gtggccacca ttacccgcct cgctgatgtg gtcaagctcg gtgcagccag cctaggagcc
     3121 gaagaccctg aaactcaggt ggtgctgatc aatgcagtaa aggacgtagc caaggccctg
     3181 ggtgacctca tcagcgctac gaaggctgca gcgggcaaag ttggggatga ccctgcagtg
     3241 tggcagctca agaactctgc caaggtgatg gtgaccaatg tgacatcatt gctcaagaca
     3301 gtgaaggctg tggaagatga ggccaccaaa ggcacacggg ccctagaggc aaccacagag
     3361 cacatacgtc aggaactggc ggtcttctgt tccccagagc cacctgccaa gacctctacc
     3421 cctgaagact tcatccgaat gaccaagggt attactatgg caacagccaa agccgttgct
     3481 gctggcaatt cctgtcgaca ggaagatgtc attgccacag ccaatctgag ccgacgggct
     3541 attgcggaca tgcttcgggc ttgcaaggaa gcagctttcc acccagaagt ggcacctgat
     3601 gtacggctcc gagccctgca ctatggccgg gagtgtgcca atggttacct ggaactgctg
     3661 gaccacgtgc tgctgaccct tcagaagcca aacccagacc tgaagcagca gctaactgga
     3721 cactcaaagc gggttgctgg ctctgtgact gagctcatcc aggctgctga agccatgaaa
     3781 ggaacagagt gggtggaccc agaggaccct actgtcattg ctgagaatga gctcttggga
     3841 gccgcagccg ccatcgaggc tgcagccaag aagctggagc agctgaagcc cagggccaaa
     3901 cccaaggagg cggatgagtc cttgaacttt gaggaacaaa tcctagaagc tgccaagtcc
     3961 atcgctgcag ccaccagtgc actggtaaag gctgcgtcag cagcccagag ggagctggtg
     4021 gctcaaggaa aggtgggcgc cattccagcc aatgcactgg atgatgggca gtggtcgcag
     4081 ggcctcattt ccgctgcccg tatggtggct gcggccacca acaatctgtg tgaagcagct
     4141 aacgcagctg tccagggcca tgctagccag gagaaactca tttcctcagc caagcaagta
     4201 gctgcctcca cagctcagct cctggtagct tgcaaggtca aagcagatca ggactctgag
     4261 gcaatgaaac ggctccaggc tgctggcaat gcagtgaaga gggcctcaga taacctggta
     4321 aaggcggccc agaaagctgc agccttcgaa gaccaggaga atgagacggt ggtggtgaag
     4381 gagaaaatgg ttgggggcat tgcccagatt atcgcagcac aggaagagat gcttcggaag
     4441 gaacgagagc tggaagaggc tcggaaaaag ctcgcccaga tccggcagca gcagtacaag
     4501 ttcttgcctt cagagcttcg agacgagcac taaaaaaccg gtgtatttaa tgcagaccca
     4561 gcccagagac tgtgcctgcc actaccaaag ccttctgggc tgctcaggac ccaagctgcc
     4621 caaccccagc actcccccaa agtgcctgcc aaaccctggg cctggccctg cccagtccca
     4681 ctgcaagccg tgtcctctcc ctgactccca agtgcctttg caccctaggg cccctaagtg
     4741 cctgcccccc tccagagtat taacgctcca agagtattat taatgctgcc gtacctcagt
     4801 ttgaacctgc cagggcccca gctgctccag cctgccagca gcttccagcc agtccccacg
     4861 gccacgtcag ctcaactcat ccctttttga tactatatcc cctacccagc tacctatggg
     4921 gcttgagggt tgtaaaccca aacaggtcag actcccggga ttctgcagtc gacggtaccg
     4981 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     5041 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     5101 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5161 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5221 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     5281 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     5341 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     5401 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     5461 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     5521 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     5581 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     5641 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     5701 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5761 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5821 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     5881 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5941 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6001 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6061 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6121 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6181 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6241 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6301 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6361 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6421 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6481 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6541 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6601 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6661 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6721 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6781 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6841 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6901 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6961 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7021 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7081 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7141 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     7201 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     7261 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     7321 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     7381 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     7441 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     7501 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7561 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7621 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7681 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7741 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7801 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7861 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7921 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7981 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8041 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8101 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8161 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8221 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8281 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tag
//
