LOCUS       sequence-402914-       11770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for bi-cistronic expression of mCherry and PC 
            (seperated by P2A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Igelmann S, Lessard F, Uchenunu O, Bouchard J, Fernandez-Ruiz A, 
            Rowell MC, Lopes-Paciencia S, Papadopoli D, Fouillen A, Ponce KJ, 
            Huot G, Mignacca L, Benfdil M, Kalegari P, Wahba HM, Pencik J, Vuong
            N, Quenneville J, Guillon J, Bourdeau V, Hulea L, Gagnon E, Kenner 
            L, Moriggl R, Nanci A, Pollak MN, Omichinski JG, Topisirovic I, 
            Ferbeyre G
  TITLE     A hydride transfer complex reprograms NAD metabolism and bypasses 
            senescence.
  JOURNAL   Mol Cell. 2021 Sep 16;81(18):3848-3865.e19. doi: 
            10.1016/j.molcel.2021.08.028.
  PUBMED    34547241
REFERENCE   2  (bases 1 to 11770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.08"; journalName: "Mol Cell"; date: 
            "2021-09-16- 16"; volume: "81"; issue: "18"; pages: "3848-3865.e19"
COMMENT     Sequence Label: pUltra-Hot-PC
FEATURES             Location/Qualifiers
     source          1..11770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2890..3007
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3072..4283
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4120..4139
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4316..4325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4322..5029
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4467..4485)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4735..4754
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5039..5095
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    8667..9255
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8720..8740)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9138..9149)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9346..9579
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9651..9772
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9688..9707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9742..9761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9812..9947
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9967..9986)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9968..9986)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9996..10013)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9996..10012)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10005..10027)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10154..10609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10241..10260)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10451..10472
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10635..10739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10740..10808,10809..11600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10958..10977)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctcact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     2881 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2941 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     3001 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta acccgtgtcg
     3061 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     3121 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     3181 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     3241 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     3301 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     3361 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     3421 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     3481 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     3541 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     3601 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     3661 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     3721 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3781 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3841 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3901 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3961 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     4021 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     4081 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     4141 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     4201 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     4261 cgtttttggc ttttttgtta gacgaagctt gggctgcagg tccgatccac cggtcgccac
     4321 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     4381 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4441 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     4501 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     4561 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     4621 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     4681 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     4741 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     4801 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     4861 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     4921 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4981 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gatctggagc
     5041 aacaaacttc tcactactca aacaagcagg tgacgtggag gagaatcccg ggccttctag
     5101 aatgctgaag ttccgaacag tccatggggg cctgaggctc ctgggaatcc gccgaacctc
     5161 caccgccccc gctgcctccc caaatgtccg gcgcctggag tataagccca tcaagaaagt
     5221 catggtggcc aacagaggtg agattgccat ccgtgtgttc cgggcctgca cggagctggg
     5281 catccgcacc gtagccatct actctgagca ggacacgggc cagatgcacc ggcagaaagc
     5341 agatgaagcc tatctcatcg gccgcggcct ggcccccgtg caggcctacc tgcacatccc
     5401 agacatcatc aaggtggcca aggagaacaa cgtagatgca gtgcaccctg gctacgggtt
     5461 cctctctgag cgagcggact tcgcccaggc ctgccaggat gcaggggtcc ggtttattgg
     5521 gccaagccca gaagtggtcc gcaagatggg agacaaggtg gaggcccggg ccatcgccat
     5581 tgctgcgggt gttcccgttg tccctggcac agatgccccc atcacgtccc tgcatgaggc
     5641 ccacgagttc tccaacacct acggcttccc catcatcttc aaggcggcct atgggggtgg
     5701 agggcgtggc atgagggtgg tgcacagcta cgaggagctg gaggagaatt acacccgggc
     5761 ctactcagag gctctggccg cctttgggaa tggggcgctg tttgtggaga agttcatcga
     5821 gaagccacgg cacatcgagg tgcagatctt gggggaccag tatgggaaca tcctgcacct
     5881 gtacgagcga gactgctcca tccagcggcg gcaccagaag gtggtcgaga ttgcccccgc
     5941 cgcccacctg gacccgcagc ttcggactcg gctcaccagc gactctgtga aactcgctaa
     6001 acaggtgggc tacgagaacg caggcaccgt ggagttcctg gtggacaggc acggcaagca
     6061 ctacttcatc gaggtcaact cccgcctgca ggtggagcac acggtcacag aggagatcac
     6121 cgacgtagac ctggtccatg ctcagatcca cgtggctgag ggcaggagcc tacccgacct
     6181 gggcctgcgg caggagaaca tccgcatcaa cgggtgtgcc atccagtgcc gggtcaccac
     6241 cgaggacccc gcgcgcagct tccagccgga caccggccgc attgaggtgt tccggagcgg
     6301 agagggcatg ggcatccgcc tggataatgc ttccgccttc caaggagccg tcatctcgcc
     6361 ccactacgac tccctgctgg tcaaagtcat tgcccacggc aaagaccacc ccacggccgc
     6421 caccaagatg agcagggccc ttgcggagtt ccgcgtccga ggtgtgaaga ccaacatcgc
     6481 cttcctgcag aatgtgctca acaaccagca gttcctggca ggcactgtgg acacccagtt
     6541 catcgacgag aacccagagc tgttccagct gcggcctgca cagaaccggg cccaaaagct
     6601 gttgcactac ctcggccatg tcatggtaaa cggtccaacc accccgattc ccgtcaaggc
     6661 cagccccagc cccacggacc ccgttgtccc tgcagtgccc ataggcccgc ccccggctgg
     6721 tttcagagac atcctgctgc gagaggggcc tgagggcttt gctcgagctg tgcggaacca
     6781 cccggggctg ctgctgatgg acacgacctt cagggacgcc caccagtcac tgctggccac
     6841 tcgtgtgcgc acccacgatc tcaaaaagat cgccccctat gttgcccaca acttcagcaa
     6901 gctcttcagc atggagaact ggggaggagc cacgtttgac gtcgccatgc gcttcctgta
     6961 tgagtgcccc tggcggcggc tgcaggagct ccgggagctc atccccaaca tccctttcca
     7021 gatgctgctg cggggggcca atgctgtggg ctacaccaac tacccagaca acgtggtctt
     7081 caagttctgt gaagtggcca aagagaatgg catggatgtc ttccgtgtgt ttgactccct
     7141 caactacttg cccaacatgc tgctgggcat ggaggcggca ggaagtgccg gaggcgtggt
     7201 ggaggctgcc atctcataca cgggcgacgt ggccgacccc agccgcacca agtactcact
     7261 gcagtactac atgggcttgg ccgaagagct ggtgcgagct ggcacccaca tcctgtgcat
     7321 caaggacatg gccgggctgc tgaagcccac ggcctgcacc atgctggtca gctccctccg
     7381 ggaccgcttc cccgacctcc cactgcacat ccacacccac gacacgtcag gggcaggcgt
     7441 ggcagccatg ctggcctgtg cccaggctgg agctgatgtg gtggatgtgg cagctgattc
     7501 catgtctggg atgacttcac agcccagcat gggggccctg gtggcctgta ccagagggac
     7561 tcccctggac acagaggtgc ccatggagcg cgtgtttgac tacagtgagt actgggaggg
     7621 ggctcgggga ctgtacgcgg ccttcgactg cacggccacc atgaagtctg gcaactcgga
     7681 cgtgtatgaa aatgagatcc cagggggcca gtacaccaac ctgcacttcc aggcccacag
     7741 catggggctt ggctccaagt tcaaggaggt caagaaggcc tatgtggagg ccaaccagat
     7801 gctgggcgat ctcatcaagg tgacgccctc ctccaagatc gtgggggacc tggcccagtt
     7861 tatggtgcag aatggattga gccgggcaga ggccgaagct caggcggaag agctgtcctt
     7921 tccccgctcc gtggtggagt tcctgcaggg ctacatcggt gtcccccatg gggggttccc
     7981 cgaacccttt cgctctaagg tactgaagga cctgccaagg gtggaggggc ggcctggagc
     8041 ctccctccct cccctggatc tgcaggcact ggagaaggag ctggtagacc ggcatgggga
     8101 ggaggtgacg ccggaagatg tgctctcagc agctatgtac cccgatgtgt ttgcccactt
     8161 caaggacttc actgccacct ttggccccct ggatagcctg aatactcgcc tcttcctgca
     8221 gggacccaag atcgcagagg agtttgaggt ggagctggag cggggcaaga cgctgcacat
     8281 caaagccctg gccgtgagcg acctgaaccg ggccggccag aggcaggtct tctttgagct
     8341 caatgggcag ctgcggtcca tcttggtcaa ggacacccag gccatgaagg agatgcactt
     8401 ccaccccaag gccctaaagg acgtgaaggg ccagatcggg gcgcccatgc ctgggaaggt
     8461 gatagacatc aaagtggtgg caggggccaa ggtggccaag ggccagcccc tgtgtgtgct
     8521 cagtgccatg aagatggaga ctgtggtgac ctcacccatg gagggtactg tccgcaaggt
     8581 tcatgtgacc aaggacatga cactggaagg tgacgacctc atcctggaga tcgagtgaga
     8641 attcgttccg gagtcgtcga ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     8701 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     8761 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     8821 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     8881 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     8941 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     9001 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     9061 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     9121 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     9181 cggctctgcg gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt
     9241 gggccgcctc cccgcctgga attaattcga gctcggtacc tttaagacca atgacttaca
     9301 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctacgta
     9361 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     9421 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9481 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9541 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     9601 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     9661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9781 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     9841 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9901 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     9961 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
    10021 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    10081 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    10141 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    10201 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    10261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    10381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10501 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    10561 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
    10621 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10681 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10741 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10801 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10861 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10921 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10981 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    11041 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    11101 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    11161 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    11221 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    11281 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    11341 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    11401 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11461 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11521 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11581 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11641 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11701 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11761 aaggatcttc
//
