LOCUS       sequence-402916-       10029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  allows the expression of the insert in Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Pocas GM, Branco-Santos J, Herrera F, Outeiro TF, Domingos PM
  TITLE     alpha-Synuclein modifies mutant huntingtin aggregation and 
            neurotoxicity in Drosophila.
  JOURNAL   Hum Mol Genet. 2015 Apr 1;24(7):1898-907. doi: 10.1093/hmg/ddu606. 
            Epub 2014 Dec 1.
  PUBMED    25452431
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddu606"; journalName: "Hum Mol Genet"; date: 
            "2015-04-1- 1"; volume: "24"; issue: "7"; pages: "1898-907"
COMMENT     Sequence Label: pUAST ɑSynWT-EGFP
FEATURES             Location/Qualifiers
     source          1..10029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1849..1871)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1971..1990
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2528..2558,2559..2760)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    2822..2916
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2935..3173
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3120..3141
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3637..4350
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             3637..4347
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(3676..3697)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3937..3956)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4284..4305
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          4441..4506
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4636..4656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4928..5062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4965..4984)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5019..5038
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5089..5107)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5559..5630,6032..6305,6380..7034,7096..7411,7615..7746,
                     7817..8431)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9210..9764,9765..9795)
                     /label=P element 5' end
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     1861 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     1921 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     1981 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcaccgaa tcgcgcggaa
     2041 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
     2101 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
     2161 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
     2221 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
     2281 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
     2341 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
     2401 gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     2461 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     2521 gccagaccat gatgaaataa cataaggtgg tcccgtcggc aagagacatc cacttaacgt
     2581 atgcttgcaa taagtgcgag tgaaaggaat agtattctga gtgtcgtatt gagtctgagt
     2641 gagacagcga tatgattgtt gattaaccct tagcatgtcc gtggggtttg aattaactca
     2701 taatattaat tagacgaaat tatttttaaa gttttatttt taataatttg cgagtacgca
     2761 aagcttggct gcatccaacg cgttgggagc tctccggatc caagcttgca tgcctgcagg
     2821 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
     2881 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcg agcgccggag
     2941 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     3001 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     3061 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     3121 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
     3181 aattgggaat tcgttaacag atctatggat gtattcatga aaggactttc aaaggccaag
     3241 gagggagttg tggctgctgc tgagaaaacc aaacagggtg tggcagaagc agcaggaaag
     3301 acaaaagagg gtgttctcta tgtaggctcc aaaaccaagg agggagtggt gcatggtgtg
     3361 gcaacagtgg ctgagaagac caaagagcaa gtgacaaatg ttggaggagc agtggtgacg
     3421 ggtgtgacag cagtagccca gaagacagtg gagggagcag ggagcattgc agcagccact
     3481 ggctttgtca aaaaggacca gttgggcaag aatgaagaag gagccccaca ggaaggaatt
     3541 ctggaagata tgcctgtgga tcctgacaat gaggcttatg aaatgccttc tgaggaaggg
     3601 tatcaagact acgaacctga agccaagctt atcgatagca agggcgagga gctgttcacc
     3661 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3721 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3781 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3841 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3901 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3961 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4021 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4081 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4141 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4201 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4261 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4321 actctcggca tggacgagct gtacaagtaa gggtacctct agaggatctt tgtgaaggaa
     4381 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     4441 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     4501 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     4561 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     4621 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     4681 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     4741 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatatttg
     4801 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     4861 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4921 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5041 tgtatcttat catgtctgga tccactagaa ggcctaattc tagtatgtat gtaagttaat
     5101 aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac tgcactggat
     5161 atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgatgtacc
     5221 aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc
     5281 ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt
     5341 cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg
     5401 gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa
     5461 aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt
     5521 gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc
     5581 tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta
     5641 catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg
     5701 tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac aaacaaatag
     5761 tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt tttgagatca
     5821 tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt tacaaaaaat
     5881 ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac
     5941 ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca taacgcttgt
     6001 agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt cgcagagctg
     6061 cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac ccagtccgcc
     6121 ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa
     6181 tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc tggtcaaccg
     6241 gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa
     6301 gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa
     6361 ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg
     6421 ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag
     6481 ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag
     6541 gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg
     6601 gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct gacctatcgg
     6661 cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac
     6721 acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg
     6781 gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga gcccacctcc
     6841 ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag
     6901 ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga gctctttgac
     6961 aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc
     7021 gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt acataacatc
     7081 tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta
     7141 cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa
     7201 gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt tgttggccac
     7261 caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg
     7321 gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact
     7381 cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata
     7441 taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg cctgattcca
     7501 cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga agataaataa
     7561 atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt gcagatggtt
     7621 gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg tgtgatgaat
     7681 atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg
     7741 ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa ctttctaatg
     7801 aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag
     7861 tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt
     7921 tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac tgcgggccgg
     7981 agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc
     8041 cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc
     8101 gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc
     8161 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
     8221 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
     8281 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
     8341 gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc
     8401 tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt
     8461 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
     8521 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
     8581 tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg
     8641 cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata
     8701 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
     8761 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
     8821 ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt
     8881 tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa
     8941 tatatttgta tatatttccc tcacagctat atttattcta atttaatatt atgacttttt
     9001 aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac
     9061 atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg
     9121 tttatatata tcaaaaataa caactataat aataagaata catttaattt agaaaatgct
     9181 tggatttcac tggaactaga attaattcgg ctgctgctct aaacgacgca tttcgtactc
     9241 caaagtacga attttttccc tcaagctctt attttcatta aacaatgaac aggacctaac
     9301 gcacagtcac gttattgttt acataaatga ttttttttac tattcaaact tactctgttt
     9361 gtgtactccc actggtatag ccttctttta tcttttctgg ttcaggctct atcactttac
     9421 taggtacggc atctgcgttg agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag
     9481 ccttccactg cgaatcatta aagtgggtat cacaaatttg ggagttttca ccaaggctgc
     9541 acccaaggct ctgctcccac aattttctct taatagcaca cttcggcacg tgaattaatt
     9601 ttactccagt cacagctttg cagcaaaatt tgcaatattt catttttttt tattccacgt
     9661 aagggttaat gttttcaaaa aaaaattcgt ccgcacacaa cctttcctct caacaagcaa
     9721 acgtgcactg aatttaagtg tatacttcgg taagcttcgg ctatcgacgg gaccacctta
     9781 tgttatttca tcatgggcca gacccacgta gtccagcggc agatcggcgg cggagaagtt
     9841 aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct
     9901 aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt tggcgtaacg
     9961 gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg agttcaagaa
    10021 gaaggcgtt
//
