LOCUS       sequence-402917-        9307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Talin(DeltaR1-12::FlnAIg1-8)-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-XT29-Cterminal-mEmerald
FEATURES             Location/Qualifiers
     source          1..9307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5245..5254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5251..5253,5254..5256,5257..5970)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5296..5317)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5557..5576)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5904..5925
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6092..6213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6129..6148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6183..6202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6220..6675)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6357..6378)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6569..6588
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6702..6806
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6808..7165
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6808..6999
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6831..6851)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7016..7151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7200..7994
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7200..7994
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7254..7273)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7864..7883
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8182..8201)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8226..8273
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8602..9190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9091..9110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgagctcaa gcttcgaatt ctagccacca tggttgcgct ttcgctgaag
      661 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      721 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      781 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      841 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcaaagaccc
      901 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      961 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
     1021 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
     1081 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1141 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1201 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccgc
     1261 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1321 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1381 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1441 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1501 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1561 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1621 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1681 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1741 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1801 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1861 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1921 aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1981 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     2041 atgccacctg cccagcagca gatcaccagt ggccagatgc accccaaact gaacccgaag
     2101 aaagcccgtg cctacgggcc aggcatcgag cccacaggca acatggtgaa gaagcgggca
     2161 gagttcactg tggaaaccag aagtgctggc cagggagagg tgctggtgta cgtggaggac
     2221 ccggccggac accaggagga ggcaaaagtg accgccaata acgacaagaa ccgcaccttc
     2281 tccgtctggt acgtccccga ggtgacgggg actcataagg ttactgtgct ctttgctggc
     2341 cagcacatcg ccaagagccc cttcgaggtg tacgtggata agtcacaggg tgacgccagc
     2401 aaagtgacag cccaaggtcc cggcctggag cccagtggca acatcgccaa caagaccacc
     2461 tactttgaga tctttacggc aggagctggc acgggcgagg tcgaggttgt gatccaggac
     2521 cccatgggac agaagggcac ggtagagcct cagctggagg cccggggcga cagcacatac
     2581 cgctgcagct accagcccac catggagggc gtccacaccg tgcacgtcac gtttgccggc
     2641 gtgcccatcc ctcgcagccc ctacactgtc actgttggcc aagcctgtaa cccgagtgcc
     2701 tgccgggcgg ttggccgggg cctccagccc aagggtgtgc gggtgaagga gacagctgac
     2761 ttcaaggtgt acacaaaggg cgctggcagt ggggagctga aggtcaccgt gaagggcccc
     2821 aagggagagg agcgcgtgaa gcagaaggac ctgggggatg gcgtgtatgg cttcgagtat
     2881 taccccatgg tccctggaac ctatatcgtc accatcacgt ggggtggtca gaacatcggg
     2941 cgcagtccct tcgaagtgaa ggtgggcacc gagtgtggca atcagaaggt acgggcctgg
     3001 ggccctgggc tggagggcgg cgtcgttggc aagtcagcag actttgtggt ggaggctatc
     3061 ggggacgacg tgggcacgct gggcttctcg gtggaagggc catcgcaggc taagatcgaa
     3121 tgtgacgaca agggcgacgg ctcctgtgat gtgcgctact ggccgcagga ggctggcgag
     3181 tatgccgttc acgtgctgtg caacagcgaa gacatccgcc tcagcccctt catggctgac
     3241 atccgtgacg cgccccagga cttccaccca gacagggtga aggcacgtgg gcctggattg
     3301 gagaagacag gtgtggccgt caacaagcca gcagagttca cagtggatgc caagcacggt
     3361 ggcaaggccc cacttcgggt ccaagtccag gacaatgaag gctgccctgt ggaggcgttg
     3421 gtcaaggaca acggcaatgg cacttacagc tgctcctacg tgcccaggaa gccggtgaag
     3481 cacacagcca tggtgtcctg gggaggcgtc agcatcccca acagcccctt cagggtgaat
     3541 gtgggagctg gcagccaccc caacaaggtc aaagtatacg gccccggagt agccaagaca
     3601 gggctcaagg cccacgagcc cacctacttc actgtggact gcgccgaggc tggccagggg
     3661 gacgtcagca tcggcatcaa gtgtgcccct ggagtggtag gccccgccga agctgacatc
     3721 gacttcgaca tcatccgcaa tgacaatgac accttcacgg tcaagtacac gccccggggg
     3781 gctggcagct acaccattat ggtcctcttt gctgaccagg ccacgcccac cagccccatc
     3841 cgagtcaagg tggagccctc tcatgacgcc agtaaggtga aggccgaggg ccctggcctc
     3901 agtcgcactg gtgtcgagct tggcaagccc acccacttca cagtaaatgc caaagctgct
     3961 ggcaaaggca agctggacgt ccagttctca ggactcacca agggggatgc agtgcgagat
     4021 gtggacatca tcgaccacca tgacaacacc tacacagtca agtacacgcc tgtccagcag
     4081 ggtccagtag gcgtcaatgt cacttatgga ggggatccca tccctaagag ccctttctca
     4141 gtggcagtat ctccaagcct ggacctcagc aagatcaagg tgtctggcct gggagagaag
     4201 gtggacgttg gcaaagacca ggagttcaca gtcaaatcaa agggtgctgg tggtcaaggc
     4261 aaagtggcat ccaagattgt gggcccctcg ggtgcagcgg tgccctgcaa ggtggagcca
     4321 ggcctggggg ctgacaacag tgtggtgcgc ttcctgcccc gtgaggaagg gccctatgag
     4381 gtggaggtga cctatgacgg cgtgcccgtg cctggcagcc cctttcctct ggaagctgtg
     4441 gagtgggtgg acccagagga ccctactgtc attgctgaga atgagctctt gggagccgca
     4501 gccgccatcg aggctgcagc caagaagctg gagcagctga agcccagggc caaacccaag
     4561 gaggcggatg agtccttgaa ctttgaggaa caaatcctag aagctgccaa gtccatcgct
     4621 gcagccacca gtgcactggt aaaggctgcg tcagcagccc agagggagct ggtggctcaa
     4681 ggaaaggtgg gcgccattcc agccaatgca ctggatgatg ggcagtggtc gcagggcctc
     4741 atttccgctg cccgtatggt ggctgcggcc accaacaatc tgtgtgaagc agctaacgca
     4801 gctgtccagg gccatgctag ccaggagaaa ctcatttcct cagccaagca agtagctgcc
     4861 tccacagctc agctcctggt agcttgcaag gtcaaagcag atcaggactc tgaggcaatg
     4921 aaacggctcc aggctgctgg caatgcagtg aagagggcct cagataacct ggtaaaggcg
     4981 gcccagaaag ctgcagcctt cgaagaccag gagaatgaga cggtggtggt gaaggagaaa
     5041 atggttgggg gcattgccca gattatcgca gcacaggaag agatgcttcg gaaggaacga
     5101 gagctggaag aggctcggaa aaagctcgcc cagatccggc agcagcagta caagttcttg
     5161 ccttcagagc ttcgagacga gcacggcgct gggtccgggt ccgggcaagg gtccggcgca
     5221 ggctccgcgt cccgatcacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     5281 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     5341 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     5401 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
     5461 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5521 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5581 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5641 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
     5701 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
     5761 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5821 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     5881 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5941 actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca
     6001 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     6061 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     6121 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6181 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
     6241 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6301 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6361 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6421 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6481 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6541 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6601 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6661 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6721 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6781 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     6841 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6901 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6961 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7081 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7141 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     7201 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     7261 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     7321 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     7381 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     7441 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     7501 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     7561 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     7621 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     7681 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     7741 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     7801 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7861 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7921 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7981 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     8041 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8101 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8161 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     8221 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     8281 cggggttcgg tcccagggct ggcactctgt cgatacccca ccaagacccc attggggcca
     8341 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     8401 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     8461 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8521 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9241 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     9301 gcattag
//
