LOCUS       sequence-402919-       10778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xbp1 tagged with HA and GFP followed by 3'UTR of xbp1spliced form.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Cairrao F, Santos CC, Le Thomas A, Marsters S, Ashkenazi A, Domingos
            PM
  TITLE     Pumilio protects Xbp1 mRNA from regulated Ire1-dependent decay.
  JOURNAL   Nat Commun. 2022 Mar 24;13(1):1587. doi: 10.1038/s41467-022-29105-x.
  PUBMED    35332141
REFERENCE   2  (bases 1 to 10778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-03-24"; volume: "13(1):1587. doi"; pages: " 
            10.1038/s41467-022-29105-x"
COMMENT     Sequence Label: pUASt-Xbp1-HA-GFP-3'UTR Spl
FEATURES             Location/Qualifiers
     source          1..10778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1204..1222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1260..1282)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1382..1401
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(1939..1969,1970..2171)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    2233..2327
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        2346..2584
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2531..2552
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3390..3416
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3396..3422
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      3434..3443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3440..3442,3443..3445,3446..4159)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3440..3442,3443..3445,3446..4156)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3485..3506)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3746..3765)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4093..4114
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          4601..4666
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4796..4816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5088..5222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5125..5144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5179..5198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5249..5267)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5719..5790,6192..6465,6540..7194,7256..7571,7775..7906,
                     7977..8591)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9370..9924,9925..9955)
                     /label=P element 5' end
     rep_origin      complement(10190..10778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10270..10289)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     1201 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     1261 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     1321 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     1381 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcaccga
     1441 atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg
     1501 gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt
     1561 atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg
     1621 tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaga
     1681 caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca
     1741 tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg caataaacag
     1801 taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg atatatgtcg
     1861 gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca
     1921 cgatgagaat ggccagacca tgatgaaata acataaggtg gtcccgtcgg caagagacat
     1981 ccacttaacg tatgcttgca ataagtgcga gtgaaaggaa tagtattctg agtgtcgtat
     2041 tgagtctgag tgagacagcg atatgattgt tgattaaccc ttagcatgtc cgtggggttt
     2101 gaattaactc ataatattaa ttagacgaaa ttatttttaa agttttattt ttaataattt
     2161 gcgagtacgc aaagcttggc tgcatccaac gcgttgggag ctctccggat ccaagcttgc
     2221 atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     2281 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactctagc
     2341 gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
     2401 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
     2461 aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc agcaaccaag
     2521 taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca agaagagaac
     2581 tctgaatagg gaattgggaa ttcatggcac ccacagcaaa cacagtgcta attaccgtgc
     2641 cgcggacagc aatcacatcg aataatctgc ccaaattgcg gcccgccccc cttacggcag
     2701 ctctgctgaa ggtgtcggcc acgccctccg cctcgcccac gccctcaagt tcgggatacg
     2761 catcctcgtc gaacatggat gacgataaca tggctgcatc ccagcccaag gccaagaagc
     2821 gtcgcctgga ccatctaacc tgggaggaga aagtgcaaag aaagaagcta aagaaccgtg
     2881 tggccgccca gacatcgcgt gaccgcaaga aggcgcgcat ggaggagatg gactacgaga
     2941 tcaaggagct gacagacaga acggaaatac tacagaacaa gtgcgatagc ctgcaggcca
     3001 tcaacgagtc actgctggcc aagaaccaca agctggactc ggagctggag ctgctgcgcc
     3061 aagaactcgc cgaactgaag cagcaacagc agcacaacac cagatgcatc agccaatcca
     3121 acgccagcgc aggcgctgag ggctgtgcgt ccaccaacct tggatctgca gcatccaaag
     3181 ctgaccctct gccgcagggt atacaacagg tggacacaca gtcgtcagcg cgtctgctgg
     3241 cggagcagct gaagagcagc aagagcctgg cctcactctg gaaagttgtg gccctctgcc
     3301 tactctacaa gacatgcttg gcgtcgacga agagttcgac gtcaagcgcc tcgaagagct
     3361 ggccgaaagt ctgctcgcag atatcttatt atccgtacga tgttccagat tatgcgagcc
     3421 tctgaccccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3481 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3541 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3601 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3661 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3721 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3781 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3841 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3901 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3961 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4021 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4081 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4141 ggacgagctg tacaagtaag gactcagatc tcgagggtac cgatctgagt ttttcaagct
     4201 gaaaacttcg ttctgtctct cttttattgt taagtttggc cggccgccgt ttgtttaagt
     4261 tttttagttg ataggctgta cataaattca taatcgaagg ctttatatat gatactgtag
     4321 ttagagcgct aagtttagct gtaggtttcc atttatattt aacaatctct aaccacatag
     4381 gcttatgatt actcttatat gtatgtattg tgctcaaaga atttgaaaaa aaaacatttg
     4441 actaaaaaat attcaaaata taaaccaaac caaacaacaa aaaaaaaaaa aaaaactcga
     4501 gttctatagt gtcacgctct agaggatctt tgtgaaggaa ccttacttct gtggtgtgac
     4561 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
     4621 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
     4681 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
     4741 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
     4801 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
     4861 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
     4921 agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg ccttgactag
     4981 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5041 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5101 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5161 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5221 tccactagaa ggcctaattc tagtatgtat gtaagttaat aaaacccatt tttgcggaaa
     5281 gtagataaaa aaaacatttt ttttttttac tgcactggat atcattgaac ttatctgatc
     5341 agttttaaat ttacttcgat ccaagggtat ttgatgtacc aggttctttc gattacctct
     5401 cactcaaaat gacattccac tcaaagtcag cgctgtttgc ctccttctct gtccacagaa
     5461 atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta
     5521 cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa
     5581 gcggtttacg ccatcaatta aacacaaagt gctgtgccaa aactcctctc gcttcttatt
     5641 tttgtttgtt ttttgagtga ttggggtggt gattggtttt gggtgggtaa gcaggggaaa
     5701 gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc tattaattcg cggaggcagc
     5761 aaacacccat ctgccgagca tctgaacaat gtgagtagta catgtgcata catcttaagt
     5821 tcacttgatc tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg
     5881 acccacaaaa atcccaaacc gcaattgcac aaacaaatag tgacacgaaa cagattattc
     5941 tggtagctgt tctcgctata taagacaatt tttgagatca tatcatgatc aagacatcta
     6001 aaggcattca ttttcgacta tattcttttt tacaaaaaat ataacaacca gatattttaa
     6061 gctgatccta gatgcacaaa aaataaataa aagtataaac ctacttcgta ggatacttcg
     6121 gggtactttt tgttcggggt tagatgagca taacgcttgt agttgatatt tgagatcccc
     6181 tatcattgca gggtgacagc ggagcggctt cgcagagctg cattaaccag ggcttcgggc
     6241 aggccaaaaa ctacggcacg ctccggccac ccagtccgcc ggaggactcc ggttcaggga
     6301 gcggccaact agccgagaac ctcacctatg cctggcacaa tatggacatc tttggggcgg
     6361 tcaatcagcc gggctccgga tggcggcagc tggtcaaccg gacacgcgga ctattctgca
     6421 acgagcgaca cataccggcg cccaggaaac atttgctcaa gaacggtgag tttctattcg
     6481 cagtcggctg atctgtgtga aatcttaata aagggtccaa ttaccaattt gaaactcagt
     6541 ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa
     6601 gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag ggcatccaag tatcgccatc
     6661 cgggatgcga ctgctcaatg gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc
     6721 ctatgtccag caggatgacc tctttatcgg ctccctaacg gccagggaac acctgatttt
     6781 ccaagccatg gtgcggatgc cacgacatct gacctatcgg cagcgagtgg cccgcgtgga
     6841 tcaggtgatc caggagcttt cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg
     6901 cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg gcattcgcct ccgaggctct
     6961 aaccgatccg ccgcttctga tctgcgatga gcccacctcc ggactggact cctttaccgc
     7021 ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac
     7081 cattcatcag ccgtcttccg agctgtttga gctctttgac aagatccttc tgatggccga
     7141 gggcagggta gctttcttgg gcactcccag cgaagccgtc gacttctttt cctagtgagt
     7201 tcgatgtgtt tattaagggt atctagtatt acataacatc tcaactccta tccagcgtgg
     7261 gtgcccagtg tcctaccaac tacaatccgg cggactttta cgtacaggtg ttggccgttg
     7321 tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa gatatgcgac aattttgcca
     7381 ttagcaaagt agcccgggat atggagcagt tgttggccac caaaaatctg gagaagccac
     7441 tggagcagcc ggagaatggg tacacctaca aggccacctg gttcatgcag ttccgggcgg
     7501 tcctgtggcg atcctggctg tcggtgctca aggaaccact cctcgtaaaa gtgcgactta
     7561 ttcagacaac ggtgagtggt tccagtggaa acaaatgata taacgcttac aattcttgga
     7621 aacaaattcg ctagatttta gatagaattg cctgattcca cacccttctt agtttttttc
     7681 aatgagatgt atagtttata gttttgcaga agataaataa atttcattta actcgcgaat
     7741 attaatgaga tgcgagtaac attttaattt gcagatggtt gccatcttga ttggcctcat
     7801 ctttttgggc caacaactca cgcaagtggg tgtgatgaat atcaacggag ccatcttcct
     7861 cttcctgacc aacatgacct ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta
     7921 gaatacattt gcatttcaat aatttactaa ctttctaatg aatcgattcg atttaggtgt
     7981 tcacctcaga gctgccagtt tttatgaggg aggcccgaag tcgactttat cgctgtgaca
     8041 catactttct gggcaaaacg attgccgaat tgccgctttt tctcacagtg ccactggtct
     8101 tcacggcgat tgcctatccg atgatcggac tgcgggccgg agtgctgcac ttcttcaact
     8161 gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc cttcggatat ctaatatcct
     8221 gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc gccggttatc ataccattcc
     8281 tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc agtatacctc aaatggttgt
     8341 cgtacctctc atggttccgt tacgccaacg agggtctgct gattaaccaa tgggcggacg
     8401 tggagccggg cgaaattagc tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg
     8461 tcatcctgga gacgcttaac ttctccgccg ccgatctgcc gctggactac gtgggtctgg
     8521 ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt cgggcccgac
     8581 gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt accattatta
     8641 ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat ttgtgccaat
     8701 aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc ccacaagtag
     8761 actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac atcaaaaact
     8821 cgtttatcgc tacataaaac accgggatat attttttata tacatacttt tcaaatcgcg
     8881 cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt tcgctgtctc
     8941 ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg actgtcgtta
     9001 gttccgcgcg attcggttcg ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa
     9061 ttagtaataa atatttgtat gtacaattta tttgctccaa tatatttgta tatatttccc
     9121 tcacagctat atttattcta atttaatatt atgacttttt aaggtaattt tttgtgacct
     9181 gttcggagtg attagcgtta caatttgaac tgaaagtgac atccagtgtt tgttccttgt
     9241 gtagatgcat ctcaaaaaaa tggtgggcat aatagtgttg tttatatata tcaaaaataa
     9301 caactataat aataagaata catttaattt agaaaatgct tggatttcac tggaactaga
     9361 attaattcgg ctgctgctct aaacgacgca tttcgtactc caaagtacga attttttccc
     9421 tcaagctctt attttcatta aacaatgaac aggacctaac gcacagtcac gttattgttt
     9481 acataaatga ttttttttac tattcaaact tactctgttt gtgtactccc actggtatag
     9541 ccttctttta tcttttctgg ttcaggctct atcactttac taggtacggc atctgcgttg
     9601 agtcgcctcc ttttaaatgt ctgacctttt gcaggtgcag ccttccactg cgaatcatta
     9661 aagtgggtat cacaaatttg ggagttttca ccaaggctgc acccaaggct ctgctcccac
     9721 aattttctct taatagcaca cttcggcacg tgaattaatt ttactccagt cacagctttg
     9781 cagcaaaatt tgcaatattt catttttttt tattccacgt aagggttaat gttttcaaaa
     9841 aaaaattcgt ccgcacacaa cctttcctct caacaagcaa acgtgcactg aatttaagtg
     9901 tatacttcgg taagcttcgg ctatcgacgg gaccacctta tgttatttca tcatgggcca
     9961 gacccacgta gtccagcggc agatcggcgg cggagaagtt aagcgtctcc aggatgacct
    10021 tgcccgaact ggggcacgtg gtgttcgacg atgtgcagct aatttcgccc ggctccacgt
    10081 ccgcccattg gttaatcagc agaccctcgt tggcgtaacg gaaccatgag aggtacgaca
    10141 accatttgag gtatactggc accgagcccg agttcaagaa gaaggcgttt ttccataggc
    10201 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10261 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10321 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10381 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10441 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10501 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10561 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10621 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10681 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10741 gcaagcagca gattacgcgc agaaaaaaag gatctcaa
//
