LOCUS       sequence-402920-       10823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  allows the expression of the insert in Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Branco-Santos J, Herrera F, Pocas GM, Pires-Afonso Y, Giorgini F, 
            Domingos PM, Outeiro TF
  TITLE     Protein phosphatase 1 regulates huntingtin exon 1 aggregation and 
            toxicity.
  JOURNAL   Hum Mol Genet. 2017 Oct 1;26(19):3763-3775. doi: 10.1093/hmg/ddx260.
  PUBMED    28934390
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2017-10-1- 1"; volume: "26"; issue: "19"; 
            pages: "3763-3775"
COMMENT     Sequence Label: pWalium Htt19Q-mCherry
FEATURES             Location/Qualifiers
     source          1..10823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(176..790,861..992,1196..1511,1573..2227,
                     2302..2575,2977..3048))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     3500..3518
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    3641..3710
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    3948..4378
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    4396..4429
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4495..4528
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4541..4635
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4659..4897
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     4844..4865
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    4942..4966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5240..5950
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5385..5403)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5653..5672
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5847..5870
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(5951..5975)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          6282..6347
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6477..6497
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6769..6890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6806..6825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6860..6879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6912..7342
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(7361..7381)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7361..7379)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7400..7416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7400..7416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7413..7435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7424..7440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7448..7454,7455..7472,7473..7478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7493..7514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7631..7648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7802..8390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7882..7901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8561..9352,9353..9421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9184..9203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9422..9526)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9553..10009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9640..9659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9850..9871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10135..10157
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10149..10166
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10150..10166
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10176..10195
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10176..10194
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caaagtctac ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt
       61 gtttttattg gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca
      121 cgatggtaat aatggtaaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact
      181 ccttgcgtcg ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga
      241 gaatggccag acccacgtag tccagcggca gatcggcggc ggagaagtta agcgtctcca
      301 ggatgacctt gcccgaactg gggcacgtgg tgttcgacga tgtgcagcta atttcgcccg
      361 gctccacgtc cgcccattgg ttaatcagca gaccctcgtt ggcgtaacgg aaccatgaga
      421 ggtacgacaa ccatttgagg tatactggca ccgagcccga gttcaagaag aagccgccaa
      481 agagcaggaa tggtatgata accggcggac ccacagacag cgccatcgag gtcgaggagc
      541 tggcgcagga tattagatat ccgaaggacg ttgacacatt ggccaccaga gtgaccagcg
      601 ccaggcagtt gaagaagtgc agcactccgg cccgcagtcc gatcatcgga taggcaatcg
      661 ccgtgaagac cagtggcact gtgagaaaaa gcggcaattc ggcaatcgtt ttgcccagaa
      721 agtatgtgtc acagcgataa agtcgacttc gggcctccct cataaaaact ggcagctctg
      781 aggtgaacac ctaaatcgaa tcgattcatt agaaagttag taaattattg aaatgcaaat
      841 gtattctaaa catgacttac atttatcgtg gcaaagacgt tttgaaaggt catgttggtc
      901 aggaagagga agatggctcc gttgatattc atcacaccca cttgcgtgag ttgttggccc
      961 aaaaagatga ggccaatcaa gatggcaacc atctgcaaat taaaatgtta ctcgcatctc
     1021 attaatattc gcgagttaaa tgaaatttat ttatcttctg caaaactata aactatacat
     1081 ctcattgaaa aaaactaaga agggtgtgga atcaggcaat tctatctaaa atctagcgaa
     1141 tttgtttcca agaattgtaa gcgttatatc atttgtttcc actggaacca ctcaccgttg
     1201 tctgaataag tcgcactttt acgaggagtg gttccttgag caccgacagc caggatcgcc
     1261 acaggaccgc ccggaactgc atgaaccagg tggccttgta ggtgtaccca ttctccggct
     1321 gctccagtgg cttctccaga tttttggtgg ccaacaactg ctccatatcc cgggctactt
     1381 tgctaatggc aaaattgtcg catatcttgg cgatccgatc acgggactcg atctcccgtc
     1441 cgggcacaac ggccaacacc tgtacgtaaa agtccgccgg attgtagttg gtaggacact
     1501 gggcacccac gctggatagg agttgagatg ttatgtaata ctagataccc ttaataaaca
     1561 catcgaactc actaggaaaa gaagtcgacg gcttcgctgg gagtgcccaa gaaagctacc
     1621 ctgccctcgg ccatcagaag gatcttgtca aagagctcaa acagctcgga agacggctga
     1681 tgaatggtca ggatgacggt cttgcccttc tgcgacagct tcttcagcac ctggacgacg
     1741 ctgtgggcgg taaaggagtc cagtccggag gtgggctcat cgcagatcag aagcggcgga
     1801 tcggttagag cctcggaggc gaatgccaga cgcttccttt ctccgccgga cagacctttc
     1861 accctgccgg gcacaccgat gatcgtgtgc tgacatttgc tgagcgaaag ctcctggatc
     1921 acctgatcca cgcgggccac tcgctgccga taggtcagat gtcgtggcat ccgcaccatg
     1981 gcttggaaaa tcaggtgttc cctggccgtt agggagccga taaagaggtc atcctgctgg
     2041 acataggcgc acctggcctg catctccttg gcgtccacag gttggccatt gagcagtcgc
     2101 atcccggatg gcgatacttg gatgccctgc ggcgatcgaa aggcaagggc attcagcagg
     2161 gtcgtctttc cggcaccgga actgcccatc acggccaaaa gttcgcccgg ataggccacg
     2221 ccgcaaactg agtttcaaat tggtaattgg accctttatt aagatttcac acagatcagc
     2281 cgactgcgaa tagaaactca ccgttcttga gcaaatgttt cctgggcgcc ggtatgtgtc
     2341 gctcgttgca gaatagtccg cgtgtccggt tgaccagctg ccgccatccg gagcccggct
     2401 gattgaccgc cccaaagatg tccatattgt gccaggcata ggtgaggttc tcggctagtt
     2461 ggccgctccc tgaaccggag tcctccggcg gactgggtgg ccggagcgtg ccgtagtttt
     2521 tggcctgccc gaagccctgg ttaatgcagc tctgcgaagc cgctccgctg tcaccctgca
     2581 atgatagggg atctcaaata tcaactacaa gcgttatgct catctaaccc cgaacaaaaa
     2641 gtaccccgaa gtatcctacg aagtaggttt atacttttat ttattttttg tgcatctagg
     2701 atcagcttaa aatatctggt tgttatattt tttgtaaaaa agaatatagt cgaaaatgaa
     2761 tgcctttaga tgtcttgatc atgatatgat ctcaaaaatt gtcttatata gcgagaacag
     2821 ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc aattgcggtt tgggattttt
     2881 gtgggtcgca gttctcacgc cgcagacaat ttgatgttgc aatcgcagtt cctatagatc
     2941 aagtgaactt aagatgtatg cacatgtact actcacattg ttcagatgct cggcagatgg
     3001 gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct tggcccattg ccgggatttt
     3061 tcacactttc ccctgcttac ccacccaaaa ccaatcacca ccccaatcac tcaaaaaaca
     3121 aacaaaaata agaagcgaga ggagttttgg cacagcactt tgtgtttaat tgatggcgta
     3181 aaccgcttgg agcttcgtca cgaaaccgct gacaaaatgc aactgaaggc ggacattgac
     3241 gctacgtaac gctacaaacg gtggcgaaag agatagcgga cgcagcggcg aaagagacgg
     3301 cgatatttct gtggacagag aaggaggcaa acagcgctga ctttgagtgg aatgtcattt
     3361 tgagtgagag gtaatcgaaa gaacctggta catcaaatac ccttggatcg aagtaaattt
     3421 aaaactgatc agataagttc aatgatatcc agtgcagtaa aaaaaaaaaa tgtttttttt
     3481 atctactttc cgcaaaaatg ggttttatta acttacatac atactaggcg cgccagatcg
     3541 caagaagctt gatatcatcg atctcgaggc tgcatccaac gcgttgggag ctctccggat
     3601 caattcggct tcaggtaccg tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
     3661 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
     3721 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
     3781 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
     3841 cggctggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
     3901 cgacaagccg aattgatcca ctagaaggcc taattcggta cactagttgg ccacgtaata
     3961 agtgtgcgtt gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt
     4021 gcatacctta tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat
     4081 tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata
     4141 ctttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt
     4201 ttttaataaa atacattgca taccctcttt taataaagaa tattgcatac gttgacgaaa
     4261 caaattttcg ttgcataccc aataaaagat tattatattg catacctttt cttgccatac
     4321 catttagccg atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacac
     4381 tagtagtacc agcttataac ttcgtataat gtatgctata cgaagttatc tgcaggcagg
     4441 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagactccc atggataact
     4501 tcgtataatg tatgctatac gaagttatgg atccgcaggt cggagtactg tcctccgagc
     4561 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     4621 gtactgtcct ccgagcggag actcgtcgac agcgagcgcc ggagtataaa tagaggcgct
     4681 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     4741 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta
     4801 aagtgaatca attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg
     4861 ccaagaagta attattgaat acaagaagag aactctgaat agggaattgg gaattccaga
     4921 tctgcggccg cggctcgaat cacaagtttg tacaaaaaag caggcttcca ccatggcgac
     4981 cctggaaaag ctgatgaagg ccttcgagtc cctcaaaagc ttccaacagc agcaacagca
     5041 acaacagcag caacagcaac aacagcagca acagcaacaa ccgccaccac ctccccctcc
     5101 acccccacct cctcaacttc ctcaacctcc tccacaggca cagcctctgc tgcctcagcc
     5161 acaacctcct ccacctccac ctccacctcc tccaggccca gctgtggctg aggagcctct
     5221 gcaccgacct gggggatcca tggtgagcaa gggcgaggag gacaacatgg ccatcatcaa
     5281 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccgcg agttcgagat
     5341 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     5401 caagggcggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     5461 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     5521 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     5581 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     5641 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     5701 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     5761 cggcggccac tacgacgccg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     5821 gcccggcgcc tacaacgtca acatcaagct ggacatcacc tcccacaacg aggactacac
     5881 catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcggca tggacgagct
     5941 gtacaagtaa acccagcttt cttgtacaaa gtggtgattc gagggtacct ctagagcaaa
     6001 ctagttctga tctgctagac aattgttggc atcaggtagg catcacacac gattaacaac
     6061 ccctaaaaat acactttgaa aatattgaaa atatgttttt gtatacattt ttgatatttt
     6121 caaataatac gcagttataa aactcattag ctaacccatt ttttctttgc ttatgcttac
     6181 agattgcaaa gaactagagc cgcgggatct ttgtgaagga accttacttc tgtggtgtga
     6241 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     6301 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     6361 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     6421 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     6481 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     6541 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     6601 aagctgcact gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta
     6661 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     6721 cacctccccc tgaacctgaa acataaaatg aatggaattg ttgttgttaa cttgtttatt
     6781 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6841 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6901 ttccagagct ctggccacgt aataagtgtg cgttgaattt attcgcaaaa acattgcata
     6961 ttttcggcaa agtaaaattt tgttgcatac cttatcaaaa aataagtgct gcatactttt
     7021 tagagaaacc aaataatttt ttattgcata cccgttttta ataaaataca ttgcataccc
     7081 tcttttaata aaaaatattg catactttga cgaaacaaat tttcgttgca tacccaataa
     7141 aagattatta tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa
     7201 agaatattgc atacgttgac gaaacaaatt ttcgttgcat acccaataaa agattattat
     7261 attgcatacc ttttcttgcc ataccattta gccgatcaat tgtgctcggc aacagtatat
     7321 ttgtggtgtg ccaaccaaca acgagctcca gcttttgttc cctttagtga gggttaattg
     7381 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7441 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7501 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7561 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7681 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8221 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8521 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8581 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8641 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8701 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8761 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8821 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8881 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8941 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9001 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9061 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9121 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9181 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9241 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9301 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9361 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9421 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9481 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9541 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     9601 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     9661 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     9721 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9781 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9841 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9901 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt
     9961 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat
    10021 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10081 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10141 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
    10201 tgggtacaag cttcacgacc taggcgcgcc ctagttccag tgaaatccaa gcattttcta
    10261 aattaaatgt attcttatta ttatagttgt tatttttgat atatataaac aacactatta
    10321 tgcccaccat ttttttgaga tgcatctaca caaggaacaa acactggatg tcactttcag
    10381 ttcaaattgt aacgctaatc actccgaaca ggtcacaaaa aattacctta aaaagtcata
    10441 atattaaatt agaataaata tagctgtgag ggaaatatat acaaatatat tggagcaaat
    10501 aaattgtaca tacaaatatt tattactaat ttctattgag acgaaatgaa ccactcggaa
    10561 ccatttgagc gaaccgaatc gcgcggaact aacgacagtc gctccaaggt cgtcgaacaa
    10621 aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta cgaaacgtgg
    10681 tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat ataaaaaata
    10741 tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc aggtgtgtaa
    10801 gtcttttggt tagaagacaa atc
//
