LOCUS       sequence-402921-       10244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bicistronic expression vector expressing CA-GFP under the CMV 
            promotor separated by an internal ribosome entry site (IRES) 
            mediated expression of dTomato reporter protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Keyan KS, Alanany R, Kohil A, Khan OM
  TITLE     E3 Ubiquitin Ligase TRIP12 Controls Exit from Mitosis via Positive 
            Regulation of MCL-1 in Response to Taxol.
  JOURNAL   Cancers (Basel). 2023 Jan 13;15(2):505. doi: 
            10.3390/cancers15020505.
  PUBMED    36672454
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2023-01-13"; volume: "15(2):505. doi"; pages: " 
            10.3390/cancers15020505"
COMMENT     Sequence Label: pDest Lenti CA-GFP
FEATURES             Location/Qualifiers
     source          1..10244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1801..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1927..1947
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2008..2311
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2312..2515
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2466..2486
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2536..2560
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2561..2570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2567..2569,2570..2572,2573..3283)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2567..2569,2570..2572,2573..3283)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2612..2633)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2873..2892)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3220..3241
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3404..3428)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3429..4003,4004..4006,4007..4015)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3596..3613)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3823..3842
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4016..4717
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(4149..4167)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4417..4436
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4611..4634
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(4721..4741)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    4754..5342
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4807..4827)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5225..5236)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5373..5872
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5408..5431)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5805..5824
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5886..6680
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5886..6680
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5940..5959)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6550..6569
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6763..6996
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7068..7189
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7105..7124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7159..7178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7229..7364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7384..7403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7385..7403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7413..7430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7413..7429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7422..7444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7571..8026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7658..7677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7868..7889
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8052..8156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8157..8225,8226..9017)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8375..8394)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9188..9776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9677..9696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9930..9947
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10064..10085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10100..10105,10106..10123,10124..10130)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10138..10154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10143..10165
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10162..10178
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10162..10178
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10197..10217
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10199..10217
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagc
     1801 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     1861 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttac
     1921 tagtatcaac tttgtataga aaagttgtag ttattaatag taatcaatta cggggtcatt
     1981 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2041 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2101 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2161 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2221 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2281 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2341 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2401 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2461 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
     2521 agtgaaccgt cagatccaag tttgtacaaa aaagcaggct gccaccatgg tgagcaaggg
     2581 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2641 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2701 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2761 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2821 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2881 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2941 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3001 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3061 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3121 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3181 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3241 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggcc gggatgaagt
     3301 ggattttcag ggcccgtgca acgatagcag cgatccgctg gtggtggcgg cgagcattat
     3361 tggcattctg catctgattc tgtggattct ggatcgcctg tgaacccagc tttcttgtac
     3421 aaagtgggcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     3481 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     3541 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3601 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3661 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3721 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3781 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3841 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3901 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     3961 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     4021 gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
     4081 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     4141 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     4201 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
     4261 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     4321 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
     4381 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     4441 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     4501 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     4561 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     4621 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     4681 ccacctgttc ctgtacggca tggacgagct gtacaagtaa caactttatt atacatagtt
     4741 gatcaattcc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4801 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4861 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4921 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4981 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5041 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5101 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     5161 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5221 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5281 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5341 gcatcgggaa ttcccgcggt tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca
     5401 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     5461 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     5521 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     5581 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     5641 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     5701 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     5761 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     5821 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctcacg
     5881 tgcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5941 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6001 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6061 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6121 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6181 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6241 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6301 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6361 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     6421 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     6481 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     6541 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     6601 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     6661 ttcttgacga gttcttctga gcgggactct gggtaccttt aagaccaatg acttacaagg
     6721 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6781 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6961 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     7021 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     7081 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7141 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     7201 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7261 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7321 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     7381 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7441 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7501 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7561 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7621 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7681 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7741 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7801 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7861 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7921 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7981 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     8041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9301 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9601 ccgaagagag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9841 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9901 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9961 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10021 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    10081 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    10141 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    10201 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaa
//
