LOCUS       sequence-402924-        2519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_38 [Paac(3)-Ia]
FEATURES             Location/Qualifiers
     source          1..2519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(85..90,91..107,108..113)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     primer_bind     complement(563..579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(576..598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(751..1339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(831..850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1472..1566)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1587..2246)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2161..2180
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2247..2349)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2436..2458
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2450..2467
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2451..2467
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagttgaca taagcctgtt cggttcgtaa actgtaatgc
      121 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
      181 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg
      241 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      301 gaatgtgaga ccaccccagg atacatagat taccacaact ccgagccctt ccacctagcg
      361 tgctaaccac gcacgctatt gttgtatccc atataatgca atcgtagtct acctgactgt
      421 acttagaaat gtggcttcgc ctttgcccac gcacctgatc gctcctcgtt tgcttttaag
      481 gaccggacga accacagagc attagaaggc taggtggagg ctcagtgatg ataagtctgc
      541 gatggtggat gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      601 gagggcacaa tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt
      661 tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag
      721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt
     1381 caacaaagcc gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcccc
     1441 ccatacgata taagttgtta ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     1501 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1561 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     1621 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1681 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     1741 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     1801 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     1861 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     1921 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1981 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     2041 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     2101 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     2161 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     2221 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     2281 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     2341 tgccgatcaa cgtctcattg atacctgaaa caaaacccat cgtacggcca aggaagtctc
     2401 caataaatgt gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac
     2461 ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
