LOCUS       sequence-402927-        2486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_57 [PtetL]
FEATURES             Location/Qualifiers
     source          1..2486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(530..546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(530..546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(543..565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(718..1306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(798..817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1439..1533)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1554..2213)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2128..2147
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2214..2316)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2403..2425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2417..2434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2418..2434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagggattt tatgaccgat gatgaagaaa agaatctgaa
      121 acttagttta tatgtggtaa aatgttttaa tcaagtttag gaggaattaa ttatgaagtg
      181 taattaatgt aacagggttc aattaaaaga gggaagcgta tcattaaccc tataaactac
      241 gtctgccctc attattggag ggtgaaataa tgtgagacca ccccaggata catagattac
      301 cacaactccg agcccttcca cctagcgtgc taaccacgca cgctattgtt gtatcccata
      361 taatgcaatc gtagtctacc tgactgtact tagaaatgtg gcttcgcctt tgcccacgca
      421 cctgatcgct cctcgtttgc ttttaaggac cggacgaacc acagagcatt agaaggctag
      481 gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt
      541 ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc tatccgacaa
      601 tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg gaaagaacat
      661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
      961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1321 cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa
     1381 atgggtaggg ggcttcaaat cgtcccccca tacgatataa gttgttacta gtgcttggat
     1441 tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1501 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1561 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     1621 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     1681 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     1741 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     1801 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     1861 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     1921 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     1981 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     2041 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     2101 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     2161 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     2221 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     2281 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattgata cctgaaacaa
     2341 aacccatcgt acggccaagg aagtctccaa taactgtgat ccaccacaag cgccagggtt
     2401 ttcccagtca cgacgttgta aaacgacggc cagtcatgca taatccgcac gcatctggaa
     2461 taaggaagtg ccattccgcc tgacct
//
