LOCUS       sequence-402929-        7892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP-Talin(DeltaR2-12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-T36-Nterminal-EGFP
FEATURES             Location/Qualifiers
     source          1..7892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      604..613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(610..612,613..615,616..1326)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(610..612,613..615,616..1326)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(655..676)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(916..935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1263..1284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4677..4798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4714..4733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4768..4787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4805..5260)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4942..4963)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5154..5173
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5287..5391
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5393..5750
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5393..5584
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5416..5436)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5601..5736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5785..6579
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5785..6579
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5839..5858)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6449..6468
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6767..6786)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6811..6858
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7187..7775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7676..7695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 atcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      781 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1021 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1081 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     1141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1201 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1261 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1321 tacaagtccg gactcagatc tcgagctcaa gctagcttcg aattcgcaga agctgccacc
     1381 atggttgcgc tttcgctgaa gattagcatt gggaatgtgg tgaagacgat gcaatttgag
     1441 ccatctacca tggtgtacga cgcctgccgc atgattcgtg agcggatccc agaggccctg
     1501 gctggccctc ccaacgactt tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc
     1561 tggctagagg ctgggaaagc tttggactac tacatgctcc gaaatgggga caccatggag
     1621 tacagaaaaa agcagagacc cctgaagata cggatgttag acggaacggt gaagactatc
     1681 atggtggatg actccaagac cgtcacagac atgctcatga ccatttgtgc ccgaattggt
     1741 atcaccaacc atgatgagta ttcactggtt cgagagctga tggaagaaaa gaaagatgag
     1801 gggacaggga ccctgagaaa agacaagacc ctactgcgag atgaaaagaa gatggagaag
     1861 ctaaagcaga agttgcacac agacgatgag ttgaactggc tggaccatgg gcggacactg
     1921 agggaacagg gagtggaaga gcatgagacg ttgctgctgc ggagaaagtt cttctactca
     1981 gaccagaatg tggattcccg agaccctgta cagctgaacc ttctctatgt gcaggcacga
     2041 gatgacatcc tgaatggctc ccatcctgtc tcctttgaca aggcctgtga atttgcaggc
     2101 ttccagtgcc agatccagtt tggacctcac aatgaacaga agcacaaggc tggcttcctt
     2161 gacctgaagg acttcctgcc caaggagtat gtgaagcaga agggagagcg taagatcttt
     2221 caggcacaca agaattgtgg gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag
     2281 ctggcccgtt ccctcaagac ttacggtgtc tccttcttcc tagtcaagga aaagatgaag
     2341 gggaagaata aactggtgcc caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg
     2401 gatgagaaga ccaaggaggt gattcaggaa tggagcctca ccaacatcaa acgctgggct
     2461 gcctctccca agagctttac tctggacttt ggagactatc aggatggcta ctactcagta
     2521 cagacgacag aaggcgagca gatcgcacag ctcattgctg gctacataga tatcatcctt
     2581 aagaagaaaa aaagcaagga ccattttggg ctggagggag atgaagagtc tactatgttg
     2641 gaggactcag tgtctcccaa aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg
     2701 aaagtggagc acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct
     2761 gagaatttcc aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg
     2821 caccgaggac acatgccacc tctgacttca gcccagcagg cgctcactgg aaccattaac
     2881 tccagcatgc aggctgtgca ggctgcccag gccactctgg atgactttga gactctaccc
     2941 cctcttggcc aggatgctgc ctccaaagct tggcgtaaga acaagatgga tgaatcaaag
     3001 catgagatcc actcccaggt agatgccatc acagctggca ctgcctctgt ggtaaacctg
     3061 acagcaggag atcctgcaga gacagactat acagcagtgg gctgtgcagt caccaccatc
     3121 tcttccaacc tgacggagat gtcccgtggg gtgaagctat tggctgccct gctggaggat
     3181 gaaggcggca atggccggcc ccttctgcaa gcagcaaagg gccttgcagg ggctgtgtcg
     3241 gaactgcttc gcagtgctca gcccgccagc gctgagcctc gtcagaacct gctgcaggca
     3301 gccgggaacg tgggccaggc cagtggggag ctgttgcagc aaattgagtg ggtggaccca
     3361 gaggacccta ctgtcattgc tgagaatgag ctcttgggag ccgcagccgc catcgaggct
     3421 gcagccaaga agctggagca gctgaagccc agggccaaac ccaaggaggc ggatgagtcc
     3481 ttgaactttg aggaacaaat cctagaagct gccaagtcca tcgctgcagc caccagtgca
     3541 ctggtaaagg ctgcgtcagc agcccagagg gagctggtgg ctcaaggaaa ggtgggcgcc
     3601 attccagcca atgcactgga tgatgggcag tggtcgcagg gcctcatttc cgctgcccgt
     3661 atggtggctg cggccaccaa caatctgtgt gaagcagcta acgcagctgt ccagggccat
     3721 gctagccagg agaaactcat ttcctcagcc aagcaagtag ctgcctccac agctcagctc
     3781 ctggtagctt gcaaggtcaa agcagatcag gactctgagg caatgaaacg gctccaggct
     3841 gctggcaatg cagtgaagag ggcctcagat aacctggtaa aggcggccca gaaagctgca
     3901 gccttcgaag accaggagaa tgagacggtg gtggtgaagg agaaaatggt tgggggcatt
     3961 gcccagatta tcgcagcaca ggaagagatg cttcggaagg aacgagagct ggaagaggct
     4021 cggaaaaagc tcgcccagat ccggcagcag cagtacaagt tcttgccttc agagcttcga
     4081 gacgagcact aaaaaaccgg tgtatttaat gcagacccag cccagagact gtgcctgcca
     4141 ctaccaaagc cttctgggct gctcaggacc caagctgccc aaccccagca ctcccccaaa
     4201 gtgcctgcca aaccctgggc ctggccctgc ccagtcccac tgcaagccgt gtcctctccc
     4261 tgactcccaa gtgcctttgc accctagggc ccctaagtgc ctgcccccct ccagagtatt
     4321 aacgctccaa gagtattatt aatgctgccg tacctcagtt tgaacctgcc agggccccag
     4381 ctgctccagc ctgccagcag cttccagcca gtccccacgg ccacgtcagc tcaactcatc
     4441 cctttttgat actatatccc ctacccagct acctatgggg cttgagggtt gtaaacccaa
     4501 acaggtcaga ctcccgggat tctgcagtcg acggtaccgc gggcccggga tccaccggat
     4561 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4621 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     4801 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4861 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4921 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4981 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     5041 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     5101 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     5161 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     5221 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     5281 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5341 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     5401 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5521 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5581 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5641 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5701 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     5761 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5821 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5881 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5941 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     6001 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6061 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6121 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6181 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6241 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6301 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6361 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6421 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6481 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6541 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6601 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6661 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6721 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     6781 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     6841 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     6901 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     6961 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     7021 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7861 ctgtggataa ccgtattacc gccatgcatt ag
//
