LOCUS       sequence-402930-        7876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Talin(DeltaR1-10)-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Liu J, Wang Y, Goh WI, Goh H, Baird MA, Ruehland S, Teo S, Bate N, 
            Critchley DR, Davidson MW, Kanchanawong P
  TITLE     Talin determines the nanoscale architecture of focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4864-73. doi: 
            10.1073/pnas.1512025112. Epub 2015 Aug 17.
  PUBMED    26283369
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1512025112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-09-1- 1"; volume: "112"; issue: "35"; pages: "E4864-73"
COMMENT     Sequence Label: Talin-T41-Cterminal-mEmerald
FEATURES             Location/Qualifiers
     source          1..7876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3814..3823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3820..3822,3823..3825,3826..4539)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3865..3886)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4126..4145)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4473..4494
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4661..4782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4698..4717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4752..4771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4789..5244)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4926..4947)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5138..5157
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5271..5375
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5377..5734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5377..5568
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5400..5420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5585..5720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5769..6563
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5769..6563
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5823..5842)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6433..6452
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6751..6770)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6795..6842
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7171..7759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7660..7679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
      601 gactcagatc tcgagctcaa gcttcgaatt ctagccacca tggttgcgct ttcgctgaag
      661 attagcattg ggaatgtggt gaagacgatg caatttgagc catctaccat ggtgtacgac
      721 gcctgccgca tgattcgtga gcggatccca gaggccctgg ctggccctcc caacgacttt
      781 gggctctttc tgtcagatga tgaccccaaa aaaggcatct ggctagaggc tgggaaagct
      841 ttggactact acatgctccg aaatggggac accatggagt acagaaaaaa gcagagaccc
      901 ctgaagatac ggatgttaga cggaacggtg aagactatca tggtggatga ctccaagacc
      961 gtcacagaca tgctcatgac catttgtgcc cgaattggta tcaccaacca tgatgagtat
     1021 tcactggttc gagagctgat ggaagaaaag aaagatgagg ggacagggac cctgagaaaa
     1081 gacaagaccc tactgcgaga tgaaaagaag atggagaagc taaagcagaa gttgcacaca
     1141 gacgatgagt tgaactggct ggaccatggg cggacactga gggaacaggg agtggaagag
     1201 catgagacgt tgctgctgcg gagaaagttc ttctactcag accagaatgt ggattcccga
     1261 gaccctgtac agctgaacct tctctatgtg caggcacgag atgacatcct gaatggctcc
     1321 catcctgtct cctttgacaa ggcctgtgaa tttgcaggct tccagtgcca gatccagttt
     1381 ggacctcaca atgaacagaa gcacaaggct ggcttccttg acctgaagga cttcctgccc
     1441 aaggagtatg tgaagcagaa gggagagcgt aagatctttc aggcacacaa gaattgtggg
     1501 cagatgagtg aaattgaggc caaggtacgc tacgtgaagc tggcccgttc cctcaagact
     1561 tacggtgtct ccttcttcct agtcaaggaa aagatgaagg ggaagaataa actggtgccc
     1621 aggctgttgg gcatcactaa ggagtgtgtg atgcgtgtgg atgagaagac caaggaggtg
     1681 attcaggaat ggagcctcac caacatcaaa cgctgggctg cctctcccaa gagctttact
     1741 ctggactttg gagactatca ggatggctac tactcagtac agacgacaga aggcgagcag
     1801 atcgcacagc tcattgctgg ctacatagat atcatcctta agaagaaaaa aagcaaggac
     1861 cattttgggc tggagggaga tgaagagtct actatgttgg aggactcagt gtctcccaaa
     1921 aagtcaacag tccttcagca gcagtacaac cgagtgggga aagtggagca cggctctgtg
     1981 gctctgccag ccatcatgcg ctctggagcc tctggtcctg agaatttcca ggtggggagc
     2041 atgccacctg cccagcagca gatcaccagt ggccagatgc accgaggtac ccaggcctgc
     2101 attacagcag ccagtgctgt gtctggtatc attgctgacc tcgacaccac catcatgttc
     2161 gctactgctg gcacacttaa ccgtgagggt gctgaaactt ttgctgacca ccgggagggt
     2221 atcttaaaga cagcaaaggt tcttgtggag gacaccaagg tcctagtgca gaatgcagct
     2281 gggagccagg agaagttggc acaagccgcc cagtcctccg tggccaccat tacccgcctc
     2341 gctgatgtgg tcaagctcgg tgcagccagc ctaggagccg aagaccctga aactcaggtg
     2401 gtgctgatca atgcagtaaa ggacgtagcc aaggccctgg gtgacctcat cagcgctacg
     2461 aaggctgcag cgggcaaagt tggggatgac cctgcagtgt ggcagctcaa gaactctgcc
     2521 aaggtgatgg tgaccaatgt gacatcattg ctcaagacag tgaaggctgt ggaagatgag
     2581 gccaccaaag gcacacgggc cctagaggca accacagagc acatacgtca ggaactggcg
     2641 gtcttctgtt ccccagagcc acctgccaag acctctaccc ctgaagactt catccgaatg
     2701 accaagggta ttactatggc aacagccaaa gccgttgctg ctggcaattc ctgtcgacag
     2761 gaagatgtca ttgccacagc caatctgagc cgacgggcta ttgcggacat gcttcgggct
     2821 tgcaaggaag cagctttcca cccagaagtg gcacctgatg tacggctccg agccctgcac
     2881 tatggccggg agtgtgccaa tggttacctg gaactgctgg accacgtgct gctgaccctt
     2941 cagaagccaa acccagacct gaagcagcag ctaactggac actcaaagcg ggttgctggc
     3001 tctgtgactg agctcatcca ggctgctgaa gccatgaaag gaacagagtg ggtggaccca
     3061 gaggacccta ctgtcattgc tgagaatgag ctcttgggag ccgcagccgc catcgaggct
     3121 gcagccaaga agctggagca gctgaagccc agggccaaac ccaaggaggc ggatgagtcc
     3181 ttgaactttg aggaacaaat cctagaagct gccaagtcca tcgctgcagc caccagtgca
     3241 ctggtaaagg ctgcgtcagc agcccagagg gagctggtgg ctcaaggaaa ggtgggcgcc
     3301 attccagcca atgcactgga tgatgggcag tggtcgcagg gcctcatttc cgctgcccgt
     3361 atggtggctg cggccaccaa caatctgtgt gaagcagcta acgcagctgt ccagggccat
     3421 gctagccagg agaaactcat ttcctcagcc aagcaagtag ctgcctccac agctcagctc
     3481 ctggtagctt gcaaggtcaa agcagatcag gactctgagg caatgaaacg gctccaggct
     3541 gctggcaatg cagtgaagag ggcctcagat aacctggtaa aggcggccca gaaagctgca
     3601 gccttcgaag accaggagaa tgagacggtg gtggtgaagg agaaaatggt tgggggcatt
     3661 gcccagatta tcgcagcaca ggaagagatg cttcggaagg aacgagagct ggaagaggct
     3721 cggaaaaagc tcgcccagat ccggcagcag cagtacaagt tcttgccttc agagcttcga
     3781 gacgagcacg gctccgcgtc ccgatcaccg gtcgccacca tggtgagcaa gggcgaggag
     3841 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3901 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3961 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac
     4021 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4081 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4141 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4201 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4261 agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4321 acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4381 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     4441 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4501 gccgggatca ctctcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     4561 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4621 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4681 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4741 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     4801 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4861 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4921 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4981 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5041 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5101 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5161 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5221 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5281 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5341 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     5401 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5461 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5581 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5641 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5701 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     5761 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5821 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5881 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5941 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6001 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6061 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6121 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6181 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6241 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     6301 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6361 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6421 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6481 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6541 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6601 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6661 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6721 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     6781 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     6841 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     6901 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     6961 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     7021 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7081 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7861 taccgccatg cattag
//
