LOCUS       sequence-402939-       11344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FKBP12-Talin(434-2541)-mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Wang Y, Barnett SFH, Le S, Guo Z, Zhong X, Kanchanawong P, Yan J
  TITLE     Label-free Single-Molecule Quantification of Rapamycin-induced 
            FKBP-FRB Dimerization for Direct Control of Cellular 
            Mechanotransduction.
  JOURNAL   Nano Lett. 2019 Oct 9;19(10):7514-7525. doi: 
            10.1021/acs.nanolett.9b03364. Epub 2019 Sep 6.
  PUBMED    31466449
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.nanolett.9b03364"; journalName: "Nano Lett"; date: 
            "2019-10-9- 9"; volume: "19"; issue: "10"; pages: "7514-7525"
COMMENT     Sequence Label: FKBP-TalinRod-mEmerald
FEATURES             Location/Qualifiers
     source          1..11344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      592..601
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(598..600,601..921)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     regulatory      7282..7291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7288..7290,7291..7293,7294..8007)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7333..7354)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7594..7613)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7941..7962
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8129..8250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8166..8185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8220..8239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8257..8712)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8394..8415)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8606..8625
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8739..8843
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8845..9202
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8845..9036
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8868..8888)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9053..9188
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9237..10031
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9237..10031
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9291..9310)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9901..9920
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10219..10238)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10263..10310
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10639..11227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11128..11147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agccaccatg
      601 ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag
      661 acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg
      721 gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa
      781 gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat
      841 gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat
      901 gtggagcttc taaaactgga aactagtgat atccagcagt acaaccgagt ggggaaagtg
      961 gagcacggct ctgtggctct gccagccatc atgcgctctg gagcctctgg tcctgagaat
     1021 ttccaggtgg ggagcatgcc acctgcccag cagcagatca ccagtggcca gatgcaccga
     1081 ggacacatgc cacctctgac ttcagcccag caggcgctca ctggaaccat taactccagc
     1141 atgcaggctg tgcaggctgc ccaggccact ctggatgact ttgagactct accccctctt
     1201 ggccaggatg ctgcctccaa agcttggcgt aagaacaaga tggatgaatc aaagcatgag
     1261 atccactccc aggtagatgc catcacagct ggcactgcct ctgtggtaaa cctgacagca
     1321 ggagatcctg cagagacaga ctatacagca gtgggctgtg cagtcaccac catctcttcc
     1381 aacctgacgg agatgtcccg tggggtgaag ctattggctg ccctgctgga ggatgaaggc
     1441 ggcaatggcc ggccccttct gcaagcagca aagggccttg caggggctgt gtcggaactg
     1501 cttcgcagtg ctcagcccgc cagcgctgag cctcgtcaga acctgctgca ggcagccggg
     1561 aacgtgggcc aggccagtgg ggagctgttg cagcaaattg gggaaagtga cactgacccc
     1621 cacttccagg atgttctaat gcagctagca aacgcagtgg caagtgctgc agccgccctg
     1681 gtcctcaagg ccaagagtgt ggcccagcgc acagaggatt ccgggcttca gacccaagtg
     1741 attgctgcag ctacgcaatg tgctctgtcc acttcccagc tggtggcctg taccaaggtg
     1801 gtagcaccta caatcagctc acctgtgtgc caagaacagc tagtcgaggc tgggcgactg
     1861 gtggccaaag ctgtggaggg ctgtgtgtct gcctcgcaag cagctacaga agatggacag
     1921 ctgcttcgag gggtaggagc agcagccacg gctgtcaccc aggccctcaa tgagctgctg
     1981 cagcacgtga aggcccacgc caccggagct gggcctgccg gtcggtatga ccaagccact
     2041 gataccatcc tcaccgtcac tgaaaacatc ttcagctcca tgggtgatgc tggggagatg
     2101 gtgcgccagg cccgcatcct ggcccaagcc acctcagacc tggtcaatgc tatcaaggcc
     2161 gatgctgagg gggagagtga tctggagaac tctagaaagc tcctgagtgc tgccaagatc
     2221 ctcgctgatg ccaccgccaa gatggtggag gcggccaagg gagcagccgc ccaccctgac
     2281 agtgaggaac agcagcagcg actgcgtgaa gcagctgagg ggcttcgcat ggccaccaat
     2341 gcagctgcgc agaacgccat caagaagaag ttggtgcagc gcctggagca tgcagccaag
     2401 caagctgcag cctctgcaac acagaccatt gctgcagccc aacatgcagc ctctgccccc
     2461 aaggcctctg cgggccccca gcccctactg gtgcagagct gtaaggccgt ggcagagcag
     2521 attccgttgc tggtgcaggg tgtccgagga agtcaagctc agcctgacag ccctagtgct
     2581 cagctcgccc tcattgctgc cagccagagc ttcctgcagc caggtgggaa gatggtggca
     2641 gcggcaaaag cctcagtgcc aacaattcag gaccaggctt cagcgatgca gctgagtcag
     2701 tgcgcaaaga acctaggcac tgccctggcc gagctgcgca ctgctgctca gaaggctcag
     2761 gaggcatgtg gacctttgga gatggattct gcactgagtg ttgtacaaaa tctagagaaa
     2821 gacctacagg aaataaaagc agcagctcga gatggcaagc ttaagccctt acctggggaa
     2881 acaatggaga agtgtaccca agatcttggc aacagcacca aagcggtgag ctctgccatc
     2941 gccaagctgc tgggggagat tgcccagggc aacgagaact atgcaggtat tgcagctcgg
     3001 gatgtagctg gtggactaag gtcactagcc caggctgcgc ggggtgtggc tgcgctgaca
     3061 tcagatcctg cagtgcaggc cattgtgctt gacacagcca gcgacgttct ggacaaggcc
     3121 agcagcctca ttgaagaggc aaaaaaagca tctggacacc caggggaccc agaaagccag
     3181 caaaggcttg ctcaggtagc taaagcagtg acccaagccc tgaaccgctg tgtcagctgc
     3241 ctgcctggcc aaagagacgt ggataatgcc ctacgagccg ttggagatgc cagcaagcga
     3301 ctcctgagtg acttgcttcc tccaagcacg gggacatttc aagaagcaca gagccgattg
     3361 aatgaagctg cagctgggtt aaatcaggcc gccacagaac tggtgcaagc ctcccgagga
     3421 actcctcagg acctggctcg ggcctccggc cgatttggac aggacttcag caccttcctg
     3481 gaagctggtg tggagatggc cggacaggcc ccgagccagg aggaccgagc ccaggtggtg
     3541 tccaacctga agggcatatc catgtcttca agcaaacttc tccttgccgc taaggccttg
     3601 tccacagacc ctgcttctcc caacctcaag agtcagctgg ctgcggctgc ccgggcagtg
     3661 acggacagca tcaaccagct catcaccatg tgcacccagc aggcacctgg ccagaaggag
     3721 tgtgacaatg cacttcggca gctagagaca gtccgagaac tcctggagaa tccagtccag
     3781 cccatcaacg acatgtccta cttcggttgc ttggacagtg tcatggagaa ctccaaggtc
     3841 ctaggtgagg ccatgactgg catctcccaa aatgccaaga atggaaatct gccggagttt
     3901 ggagatgcca ttgccacagc ctccaaggct ctctgtggct tcacggaggc agccgcacag
     3961 gcagcatatc tagttggtgt ctctgacccc aacagccaag ctggacagca aggactggtg
     4021 gaacccacac agtttgcccg tgcaaaccag gcaattcaga tggcctgtca gagtctgggg
     4081 gagcctggct gtacccaggc ccaggtgtta tctgcagcca ctattgtagc caaacacaca
     4141 tctgcattgt gtaacagctg tcgcctggct tccgctagga ctgccaatcc cactgccaag
     4201 cgccagtttg tacagtcagc caaggaggtg gccaacagta cagccaatct tgtcaagacc
     4261 atcaaggcac tagatgggga cttcacagaa gagaaccgtg cccagtgccg agcagccaca
     4321 gcccctctgc tggaagctgt ggataacctg agtgcctttg cctccaaccc tgagttctcc
     4381 agcgtccctg cccagatcag ccctgagggc agagcagcca tggagcccat tgtaatctct
     4441 gctaagacaa tgttggagag tgctggaggg ctcatccaga cagctcgggc gttagcagtc
     4501 aatccccgag accccccacg ttggtctgtg ctagctggcc actctcggac tgtctcagat
     4561 tccatcaaga aacttattac aagcatgagg gacaaagccc cagggcagct ggagtgtgag
     4621 acagccattg cggctctgaa cagctgcttg cgggacctag atcaggcttc gcttgccgct
     4681 gtcagccagc agcttgctcc ccgtgaagga atctctcaag aggctttgca cacccagatg
     4741 ctcactgcag tgcaagaaat ttctcatctc attgagccgc tggccagcgc tgctcgggct
     4801 gaagcctccc agctgggaca caaggtgtcc caaatggccc agtactttga accactcacc
     4861 ctggctgcag tgggtgctgc gtctaagacc ctgagccacc cgcagcagat ggcacttctg
     4921 gaccagacta aaacgttggc agagtctgcc ttgcagttgc tatatactgc caaggaggct
     4981 ggtggtaacc ccaagcaagc agcacacacc caggaagccc tggaggaggc tgtgcagatg
     5041 atgacagagg ccgtagaaga cctgacgacg accctcaacg aagcagccag tgctgcagga
     5101 gtcgttggcg gcatggtgga ctctatcact caggccatca accagctaga tgaaggacct
     5161 atgggtgacc cagaaggctc attcgtagat taccagacaa ccatggtgag gacagccaag
     5221 gccattgctg tcactgttca ggagatggta accaagtcaa acaccagccc tgaagagcta
     5281 ggccctcttg ccaaccagct gaccagtgac tatggccgac tggcctcaca agccaagcct
     5341 gcagctgtgg ctgctgaaaa tgaagagata ggcgctcaca tcaaacaccg agtacaggag
     5401 ctgggccatg gctgctctgc tctggtcacc aaggcaggtg ccttgcagtg tagccccagt
     5461 gatgtctaca ccaagaagga gctcatagag tgtgcccgca gagtgtcaga gaaggtctcc
     5521 catgttctgg ctgcactcca ggctgggaat cgtggtaccc aggcctgcat tacagcagcc
     5581 agtgctgtgt ctggtatcat tgctgacctc gacaccacca tcatgttcgc tactgctggc
     5641 acacttaacc gtgagggtgc tgaaactttt gctgaccacc gggagggtat cttaaagaca
     5701 gcaaaggttc ttgtggagga caccaaggtc ctagtgcaga atgcagctgg gagccaggag
     5761 aagttggcac aagccgccca gtcctccgtg gccaccatta cccgcctcgc tgatgtggtc
     5821 aagctcggtg cagccagcct aggagccgaa gaccctgaaa ctcaggtggt gctgatcaat
     5881 gcagtaaagg acgtagccaa ggccctgggt gacctcatca gcgctacgaa ggctgcagcg
     5941 ggcaaagttg gggatgaccc tgcagtgtgg cagctcaaga actctgccaa ggtgatggtg
     6001 accaatgtga catcattgct caagacagtg aaggctgtgg aagatgaggc caccaaaggc
     6061 acacgggccc tagaggcaac cacagagcac atacgtcagg aactggcggt cttctgttcc
     6121 ccagagccac ctgccaagac ctctacccct gaagacttca tccgaatgac caagggtatt
     6181 actatggcaa cagccaaagc cgttgctgct ggcaattcct gtcgacagga agatgtcatt
     6241 gccacagcca atctgagccg acgggctatt gcggacatgc ttcgggcttg caaggaagca
     6301 gctttccacc cagaagtggc gcctgatgta cggctccgag ccctgcacta tggccgggag
     6361 tgtgccaatg gttacctgga actgctggac cacgtgctgc tgacccttca gaagccaaac
     6421 ccagacctga agcagcagct aactggacac tcaaagcggg ttgctggctc tgtgactgag
     6481 ctcatccagg ctgctgaagc catgaaagga acagagtggg tggacccaga ggaccctact
     6541 gtcattgctg agaatgagct cttgggagcc gcagccgcca tcgaggctgc agccaagaag
     6601 ctggagcagc tgaagcccag ggccaaaccc aaggaggcgg atgagtcctt gaactttgag
     6661 gaacaaatcc tagaagctgc caagtccatc gctgcagcca ccagtgcact ggtaaaggct
     6721 gcgtcagcag cccagaggga gctggtggct caaggaaagg tgggcgccat tccagccaat
     6781 gcactggatg atgggcagtg gtcgcagggc ctcatttccg ctgcccgtat ggtggctgcg
     6841 gccaccaaca atctgtgtga agcagctaac gcagctgtcc agggccatgc tagccaggag
     6901 aaactcattt cctcagccaa gcaagtagct gcctccacag ctcagctcct ggtagcttgc
     6961 aaggtcaaag cagatcagga ctctgaggca atgaaacggc tccaggctgc tggcaatgca
     7021 gtgaagaggg cctcagataa cctggtaaag gcggcccaga aagctgcagc cttcgaagac
     7081 caggagaatg agacggtggt ggtgaaggag aaaatggttg ggggcattgc ccagattatc
     7141 gcagcacagg aagagatgct tcggaaggaa cgagagctgg aagaggctcg gaaaaagctc
     7201 gcccagatcc ggcagcagca gtacaagttc ttgccttcag agcttcgaga cgagcacggc
     7261 tccgcgtccc gatcaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     7321 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     7381 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     7441 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc
     7501 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     7561 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     7621 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     7681 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc
     7741 tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac
     7801 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     7861 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     7921 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     7981 ctcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
     8041 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     8101 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     8161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8221 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     8281 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     8341 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     8401 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     8461 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     8521 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     8581 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     8641 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     8701 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8761 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8821 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     8881 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8941 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9001 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9061 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9121 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9181 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     9241 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     9301 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     9361 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     9421 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     9481 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     9541 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     9601 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     9661 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     9721 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     9781 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     9841 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     9901 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     9961 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
    10021 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
    10081 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
    10141 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
    10201 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
    10261 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
    10321 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
    10381 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
    10441 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
    10501 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10561 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10621 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10681 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10741 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10801 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10861 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10921 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10981 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    11041 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    11101 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    11161 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    11221 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    11281 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
    11341 ttag
//
