LOCUS       sequence-402978-        9638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing ID2-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-ID2-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..9638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..92)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        264..643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        645..843
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             861..1041
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1088..1213
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1706..1939
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2124..2168
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2466..2583
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2640..2658,2659..2681,2682..2700,2701..2723,2724..2742,
                     2743..2765,2766..2784,2785..2807,2808..2826,2827..2849,
                     2850..2868,2869..2891,2892..2910)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2943..2981
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2978..3002
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3514..3570
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3580..4287
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3722..3740)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3990..4009
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4310..4898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4363..4383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4781..4792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4901..4917)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4902..4918)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5469..5649
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5675..5692)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5681..5905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5951..6379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6038..6057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6248..6269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6388..6408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6393..6722
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6573..6708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6635..6654
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6770..6817
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6836..7210
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7340..7461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7377..7396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7431..7450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7510..7526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7510..7526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7523..7545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7534..7550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7558..7564,7565..7582,7583..7588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7603..7624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7741..7758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7912..8500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7992..8011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8671..9462,9463..9531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9294..9313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9532..9636)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
       61 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
      121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
      181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      241 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      301 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      361 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      421 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      481 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      541 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      601 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      661 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      721 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      781 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      841 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      901 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      961 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     1021 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     1081 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     1141 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     1201 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     1261 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     1321 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     1381 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1441 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1501 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     1561 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1621 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1681 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1741 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1801 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1861 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1921 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1981 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     2041 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     2101 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     2161 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     2221 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     2281 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     2341 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2401 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2461 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2521 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2581 tttcgggttt attacaggga cagcagagat ccagtttggt tagatctcga gtttaccact
     2641 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2701 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2761 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2821 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2881 gagtttacca ctccctatca gtgatagaga aaagtggaag tcgagctcgg tacccgggtc
     2941 gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
     3001 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     3061 gcctccgcgg ccccgaattc cgccaccatg aaagccttca gtcccgtgag gtccgttagg
     3121 aaaaacagcc tgtcggacca cagcctgggc atctcccgga gcaaaacccc tgtggacgac
     3181 ccgatgagcc tgctatacaa catgaacgac tgctactcca agctcaagga gctggtgccc
     3241 agcatccccc agaacaagaa ggtgagcaag atggaaatcc tgcagcacgt catcgactac
     3301 atcttggacc tgcagatcgc cctggactcg catcccacta ttgtcagcct gcatcaccag
     3361 agacccgggc agaaccaggc gtccaggacg ccgctgacca ccctcaacac ggatatcagc
     3421 atcctgtcct tgcaggcttc tgaattccct tctgagttaa tgtcaaatga cagcaaagca
     3481 ctgtgtggca cgcgtgaatt cgatggatcc ggggccacca acttttccct gctgaagcag
     3541 gctggcgacg tcgaggagaa ccctggccct ggctccggag tgagcaaggg cgaggaggat
     3601 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     3661 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3721 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     3781 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     3841 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     3901 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     3961 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     4021 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     4081 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     4141 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     4201 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     4261 ggcggcatgg acgagctgta caagtgaatc tagaacgcgt taagtcgaca atcaacctct
     4321 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4381 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4441 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4501 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4561 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4621 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4681 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4741 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4801 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4861 tcagacgagt cggatctccc tttgggccgc ttccccgcat cgataccgtc gacctcgaga
     4921 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     4981 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     5041 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5101 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     5161 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     5221 actgaccttt ggatggtgct acaaggctag tgtcaaaaca ggaattgaga ccgtccttta
     5281 atctagcaga actagtacca gttgagcaag aggaggtaga agaagccaat gaaggagaga
     5341 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     5401 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     5461 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5521 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5581 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5641 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5701 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5761 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5821 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5881 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5941 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6001 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6061 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6121 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6181 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6241 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6301 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6361 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6541 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6661 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6721 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     6781 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     6841 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     6901 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     6961 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     7021 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     7081 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     7141 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     7201 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7261 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7321 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7441 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9421 ctgatcttca gcatctttta cttttaccag cgtttctggg tgagcaaaaa caggaaggca
     9481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9601 atgtatttag aaaaataaac aaataggggt tccgcgca
//
