LOCUS       sequence-402984-       10313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-EGR3-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..58)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        230..609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..809
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             827..1007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1054..1179
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1672..1905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2090..2134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2432..2549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2606..2624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2648..2666
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2690..2708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2732..2750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2825..2863
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2860..2884
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4155..4211
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4221..4928
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4363..4381)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4631..4650
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4951..5539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5004..5024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5422..5433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5542..5558)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5543..5559)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6110..6290
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6316..6333)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6322..6546
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6592..7020
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6679..6698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6889..6910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7029..7049)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7034..7363
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7214..7349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7276..7295
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7411..7458
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7477..7851
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7981..8102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8018..8037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8072..8091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8151..8167)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8151..8167)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8164..8186)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8175..8191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8199..8205,8206..8223,8224..8229))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8244..8265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8382..8399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8553..9141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8633..8652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9312..10103,10104..10172))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9935..9954
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10173..10277)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
       61 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      121 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      181 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      241 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      301 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      361 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      421 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      481 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      541 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      601 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      661 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      721 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      781 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
      841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1021 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1081 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1141 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1201 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1261 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1321 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1381 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1441 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1501 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1561 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1621 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1681 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1741 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1801 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1861 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1921 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1981 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2041 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2101 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2161 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2221 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2281 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2341 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     2401 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2461 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2521 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2581 tttggttaga tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     2641 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     2701 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2761 tcgagtttac cactccctat cagtgataga gaaaagtgga agtcgagctc ggtacccggg
     2821 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     2881 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     2941 cagcctccgc ggccccgaat tccgccacca tgaccggcaa actcgccgag aagctgccgg
     3001 tgaccatgag cagtttgcta aaccaactgc ctgacaatct gtaccccgag gagatcccca
     3061 gcgcgctcaa cctcttctcc ggcagcagcg actcggtagt ccattacaat cagatggcta
     3121 cagagaatgt aatggacatc ggtctgacca acgagaagcc caacccggaa ctctcttact
     3181 ccggctcctt ccagccagcc cccggcaaca agaccgtgac ctacttggga aagttcgcct
     3241 tcgactcccc ttccaactgg tgccaggaca acatcattag cctcatgagc gccggcatct
     3301 tgggggtgcc cccggcttca ggggcgctca gcacgcagac gtccacggcc agcatggtgc
     3361 agccaccgca gggtgacgtg gaggccatgt atcccgcgct acccccctac tccaactgcg
     3421 gcgacctcta ctcagagccc gtgtctttcc acgaccccca gggcaatccc gggctcgcct
     3481 attcccccca ggattaccaa tcggccaagc cggcgttgga cagcaatctc ttccccatga
     3541 ttcctgacta caacctctac caccacccca acgacatggg ctccattccg gagcacaagc
     3601 ccttccaggg catggacccc atccgggtca acccgccccc tattacccct ctggagacca
     3661 tcaaggcatt caaagacaag cagatccacc cgggctttgg cagcctgccc cagccgccgc
     3721 tcaccctcaa gcccatccgg ccccgcaagt accccaaccg gcctagcaag acaccgctcc
     3781 acgaacggcc ccacgcgtgc ccggccgagg gctgcgaccg ccgtttcagc cgttcggacg
     3841 agctgacccg gcacctgcgc atccacacgg gccacaagcc cttccagtgc cggatctgca
     3901 tgcggagctt cagccgcagc gaccacctca ccactcacat ccgcactcat acgggcgaga
     3961 agccctttgc ctgcgagttc tgcgggcgca agtttgcgcg cagcgacgag cgcaagcgcc
     4021 acgccaagat ccacctcaag caaaaggaga agaaggcgga gaagggcggt gcaccctctg
     4081 catcctcggc gccccccgtg tcgctggccc ccgtggtcac cacctgcgcc acgcgtgaat
     4141 tcgatggatc cggggccacc aacttttccc tgctgaagca ggctggcgac gtcgaggaga
     4201 accctggccc tggctccgga gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     4261 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     4321 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     4381 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     4441 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     4501 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     4561 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     4621 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     4681 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     4741 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     4801 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     4861 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     4921 acaagtgaat ctagaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     4981 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5041 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5101 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5161 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5221 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     5281 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     5341 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     5401 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5461 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5521 ctttgggccg cttccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca
     5581 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5641 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     5701 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5761 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     5821 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     5881 tacaaggcta gacaatacac attctgttgg tcgtagagcg cagctagcag aactagtacc
     5941 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     6001 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     6061 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     6121 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6181 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6241 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     6301 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6361 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6421 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6481 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6541 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     6601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6721 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7021 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7081 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7201 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7261 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7321 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7381 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7441 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7501 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7561 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     7621 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     7681 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     7741 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     7801 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     7861 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7921 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7981 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8041 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8101 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8161 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8221 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8281 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8341 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8401 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8461 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8521 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8581 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8641 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8761 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8881 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8941 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9001 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9061 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9121 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9181 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9241 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9301 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9361 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9421 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9481 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9541 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9601 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9661 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9721 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9781 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9841 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9901 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9961 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10021 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10081 acttttacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10141 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10201 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10261 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cgg
//
