LOCUS       sequence-402988-       10358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 10358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-PBX4-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(33..52)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        224..603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        605..803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             821..1001
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1048..1173
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1666..1899
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2084..2128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2426..2543
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2600..2618,2619..2641,2642..2660,2661..2683,2684..2702,
                     2703..2725,2726..2744,2745..2767,2768..2786,2787..2809,
                     2810..2828,2829..2851,2852..2870)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2903..2941
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2938..2962
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3042..3051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4194..4250
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4260..4967
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4402..4420)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4670..4689
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4990..5578
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5043..5063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5461..5472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5581..5597)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5582..5598)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6149..6329
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6355..6372)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6361..6585
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6631..7059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6718..6737)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6928..6949
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7068..7088)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7073..7402
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7253..7388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7315..7334
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7450..7497
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7516..7890
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8020..8141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8057..8076)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8111..8130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8190..8206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8190..8206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8203..8225)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8214..8230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8238..8244,8245..8262,8263..8268))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8283..8304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8421..8438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8592..9180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8672..8691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9351..10142,10143..10211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9974..9993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10212..10316)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
       61 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
      121 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
      181 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      241 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      301 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      361 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      421 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      481 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      541 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      601 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      661 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      721 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      781 cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca
      841 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      901 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      961 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     1021 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     1081 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     1141 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     1201 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     1261 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1321 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1381 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1441 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1501 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1561 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1621 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1681 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1741 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1801 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1861 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1921 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1981 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     2041 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     2101 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     2161 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     2221 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     2281 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2341 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     2401 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     2461 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     2521 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     2581 tagatctcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2641 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2701 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     2761 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     2821 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtggaag
     2881 tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
     2941 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     3001 aagacaccgg gaccgatcca gcctccgcgg ccccgaattc cgccaccatg gccgccccgc
     3061 cgcgccccgc gccatcgccc cccgccccgc ggcgcctcga cacgagcgac gtcctgcagc
     3121 agatcatggc catcaccgac cagagcctgg acgaggcaca ggccagaaag catgctctga
     3181 attgccatcg gatgaagcct gctctgttca gcgtgctctg tgagatcaag gaaaagacag
     3241 tggtaagcat ccgtggcatt caagacgaag atccccctga cgcccagctc ctgaggctgg
     3301 ataacatgct gctggctgag ggcgtgtgca ggcccgagaa gagaggaaga ggaggagcgg
     3361 tggccagggc cggcacagca acaccaggtg gctgtccaaa tgacaatagc attgagcact
     3421 ctgactacag ggccaagctg tcccagatcc gacagattta ccactctgag ctagagaaat
     3481 atgaacaggc ctgtcgtgag ttcaccacgc acgtcaccaa cctcctccag gagcagagca
     3541 ggatgaggcc tgtctcccct aaggagattg agcgcatggt cggcgccatt cacggcaagt
     3601 tcagcgccat ccagatgcag ttgaagcaga gcacctgtga ggcagtgatg accctgcgtt
     3661 cgcggctgct cgatgccagg cgcaagcggc ggaatttcag caagcaggcg acggaagtgc
     3721 tgaatgagta tttttactcc catctgaaca acccttaccc cagcgaagaa gccaaagaag
     3781 agctggccag gaagggcggc ctcaccatct cccaggtctc taactggttt ggcaacaaaa
     3841 gaatccggta taaaaagaac atggggaagt ttcaagaaga ggctaccatt tacacgggta
     3901 aaacggctgt ggataccacg gaagttgggg tcccagggaa ccacgccagc tgcctgtcaa
     3961 cacctagctc cggctcctct ggacccttcc cgctgcccag cgctggggac gccttcctca
     4021 ccctgcggac tctggcctct ctccagcctc ctcctggggg aggctgcctg cagtcccagg
     4081 cccagggtag ctggcagggg gccacccccc aacctgcaac tgcctcacct gctggagacc
     4141 ctggcagcat caactccagt acatctaata cgcgtgaatt cgatggatcc ggggccacca
     4201 acttttccct gctgaagcag gctggcgacg tcgaggagaa ccctggccct ggctccggag
     4261 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     4321 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4381 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     4441 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     4501 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4561 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     4621 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     4681 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     4741 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     4801 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     4861 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     4921 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgaatc tagaacgcgt
     4981 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5041 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5101 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5161 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5221 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5281 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5341 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     5401 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5461 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5521 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ttccccgcat
     5581 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     5641 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     5701 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5761 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     5821 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     5881 gggccaggga tcagatatcc actgaccttt ggatggtgct acaaggctag cagcttggta
     5941 ggccacggtc cgatacctta cactagcaga actagtacca gttgagcaag agaaggtaga
     6001 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     6061 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     6121 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     6181 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6241 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6301 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     6361 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6421 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6481 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6541 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     6601 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     6661 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6721 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6781 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6841 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6901 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6961 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7021 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     7081 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7141 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7201 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7321 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7381 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7441 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7501 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     7561 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     7621 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     7681 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7741 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7801 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     7861 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     7921 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7981 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8041 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8101 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8161 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8221 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8281 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8341 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9001 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10081 taacccactc gtgcacccaa ctgatcttca gcatctttta cttttaccag cgtttctggg
    10141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10321 tttccccgaa aagtgccacc tgacgtcgac ggatcggg
//
