LOCUS       sequence-402989-       12794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-ZEB2-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..12794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(70..89)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        261..640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..840
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             858..1038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1085..1210
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1703..1936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2121..2165
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2463..2580
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2637..2655
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2679..2697
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2721..2739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2763..2781
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2805..2823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2856..2894
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2891..2915
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6667..6723
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6733..7440
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6875..6893)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7143..7162
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7463..8051
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7516..7536)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7934..7945)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8054..8070)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8055..8071)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8622..8802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8828..8845)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8834..9058
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9104..9532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9191..9210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9401..9422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9541..9561)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9546..9875
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9726..9861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9788..9807
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9923..9970
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9989..10363
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10493..10614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10530..10549)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10584..10603
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10663..10679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10663..10679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10676..10698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10687..10703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10711..10717,10718..10735,10736..10741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10756..10777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10894..10911)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11065..11653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11145..11164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11824..12615,12616..12684))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12447..12466
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12685..12789)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
       61 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
      121 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
      181 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
      241 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      901 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      961 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1021 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     1081 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     1141 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     1201 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1261 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1321 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1381 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1441 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1501 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     1561 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1621 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1681 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1741 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1801 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1861 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1921 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1981 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     2041 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2101 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2161 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2221 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2281 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2341 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2401 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     2461 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2521 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2581 cgggtttatt acagggacag cagagatcca gtttggttag atctcgagtt taccactccc
     2641 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2701 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2761 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2821 agaaaagtgg aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2881 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     2941 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttccgccacc
     3001 atgaagcagc cgatcatggc ggatggcccc cggtgcaaga ggcgcaaaca agccaatccc
     3061 aggaggaaaa acgtggtgaa ctatgacaat gtagtggaca caggttctga aacagatgag
     3121 gaagacaagc ttcatattgc tgaggatgac ggtattgcca accctctgga ccaggagacg
     3181 agtccagcta gtgtgcccaa ccatgagtcc tccccacacg tgagccaagc tctgttgcca
     3241 agagaggaag aggaagatga aataagggag ggtggagtgg aacacccctg gcacaacaac
     3301 gagattctac aagcctctgt agatggtcca gaagaaatga aggaagacta tgacactatg
     3361 gggccagaag ccacgatcca gaccgcaatt aacaatggta cagtgaagaa tgcaaattgc
     3421 acatcagatt ttgaggaata ctttgccaaa agaaaactgg aggaacgcga tggtcatgca
     3481 gtcagcatcg aggagtacct tcagcgcagt gacacagcca ttatttaccc agaagcccct
     3541 gaggagctgt ctcgccttgg cacgccagag gccaatgggc aagaagaaaa tgacctgcca
     3601 cctggaactc cagatgcttt tgcccaactg ctgacctgcc cctactgcga ccggggctac
     3661 aagcgcttga catcactgaa ggagcacatc aagtaccgcc acgagaagaa tgaagagaac
     3721 ttttcctgcc ctctctgtag ctacacgttt gcctaccgca cccagctcga gcggcatatg
     3781 gtgacacaca agccagggac agatcagcac caaatgctaa cccaaggagc aggtaatcgc
     3841 aagttcaaat gcacagagtg tggcaaggcc ttcaaatata aacaccatct gaaagaacac
     3901 ctgcgaattc acagtggtga aaaaccttac gagtgcccaa actgcaagaa acgtttctcc
     3961 cattctggtt cctacagttc gcacatcagc agcaagaaat gtattggttt aatctctgta
     4021 aatggccgaa tgagaaacaa tatcaagacg ggttcttccc ctaattctgt ttcttcttct
     4081 cctactaatt cagccattac ccagttaaga aacaagttgg agaatggaaa accacttagt
     4141 atgtctgaac agacaggctt acttaaaatt aaaacagaac cactagactt caatgactat
     4201 aaagttctta tggctacaca cgggtttagt ggcactagtc cctttatgaa tggtgggctt
     4261 ggagccacca gccctttagg agttcatcca tctgctcaga gtccaatgca gcacttaggt
     4321 gtagggatgg aagccccttt acttgggttt cccaccatga atagtaattt aagtgaggta
     4381 caaaaggttc tacagattgt ggacaatact gtttccaggc aaaaaatgga ctgcaaggct
     4441 gaagaaattt caaagttgaa aggttatcac atgaaggatc catgctctca acctgaggaa
     4501 caaggagtta cttctcctaa tattccgcct gtcggtcttc cggtagtgag tcataatggt
     4561 gccactaaaa gtattattga ctatacgttg gaaaaagtca atgaagccaa agcttgcctc
     4621 cagagcttga ctactgactc aaggagacag atcagtaata taaagaaaga gaagctacgt
     4681 actttaatag atttggtcac tgatgacaaa atgattgaga accacaacat atccactcca
     4741 ttttcatgcc agttctgtaa agaaagtttt cctggcccca tccctttgca tcagcatgaa
     4801 cgttaccttt gtaagatgaa tgaagagatc aaggcggtcc tgcagcctca tgaaaacata
     4861 gtccccaaca aagccggagt ttttgttgat aataaagccc tcctcttgtc atctgtactt
     4921 tctgagaaag gaatgacaag ccccatcaac ccatacaagg accacatgtc tgtactcaaa
     4981 gcatactatg ctatgaacat ggagcccaac tccgatgaac tgctgaaaat ttccattgct
     5041 gtgggccttc ctcaggaatt tgtgaaggaa tggtttgaac aacgaaaagt ctaccagtac
     5101 tcaaattcca ggtccccatc cctggaaaga agctccaagc cgttagctcc caacagtaac
     5161 cctcccacaa aagactcttt attacccagg tctcctgtaa aacctatgga ctccataaca
     5221 tcaccatcta tagcagaact ccacaacagt gttacgaatt gtgatcctcc tctcaggcta
     5281 acaaaacctt cccattttac caatattaaa ccagttgaaa aattggacca ctccaggagt
     5341 aatactcctt ctcccttaaa tctttcctcc acatcttcta aaaactccca cagtagttca
     5401 tacactccaa acagcttctc ttctgaggag ctccaggctg agcctttaga cttgtcatta
     5461 ccaaaacaaa tgaaagaacc caaaagtatt atagccacaa agaacaaaac aaaagctagt
     5521 agcatcagtt tagatcataa cagtgtttct tcctcatctg aaaactcaga tgagcctctg
     5581 aacttgactt ttatcaagaa ggaattttca aattcaaata atctggacaa caaaagcact
     5641 aacccagtgt tcagcatgaa cccatttagt gccaaacctt tatacacagc tcttccacct
     5701 caaagcgcat ttccccctgc tactttcatg ccaccagtcc agaccagtat tcctgggcta
     5761 cgaccatacc caggactgga tcagatgagc ttcctaccac atatggccta cacctaccca
     5821 actggagcag ctacttttgc tgatatgcag caaaggagaa agtaccagcg gaaacaagga
     5881 tttcagggag aattgcttga tggagcacaa gactacatgt caggcctaga tgatatgaca
     5941 gactccgact cctgtctgtc tcgcaaaaag atcaagaaga cagagagtgg catgtatgca
     6001 tgtgacttat gtgacaagac attccagaaa agcagttccc ttctgcgaca taaatacgaa
     6061 cacacaggaa aaagaccaca tcagtgtcag atttgtaaga aagcgtttaa acacaagcac
     6121 caccttatcg agcactcaag gcttcactcg ggcgagaagc cctatcagtg tgataaatgt
     6181 ggcaagcgct tctcacactc gggctcgtac tcgcagcaca tgaatcacag gtattcctac
     6241 tgcaagcggg aggcggagga gcgggaagcg gcggagcgcg aggcgcgcga gaaagggcac
     6301 ttggaaccca ccgagctgct gatgaaccgg gcttacttgc agagcattac ccctcagggg
     6361 tactctgact cggaggagag ggagagtatg ccgagggatg gcgagagcga gaaggagcac
     6421 gagaaagaag gcgaggatgg ctacgggaag ctgggcagac aggatggcga cgaggagttc
     6481 gaggaggaag aggaagaaag tgaaaataaa agtatggata cggatcccga aacgatacga
     6541 gatgaagaag agactggaga tcactccatg gacgatagtt cggaggatgg gaaaatggaa
     6601 accaaatcag accacgagga agacaatatg gaagatggca tgacgcgtga attcgatgga
     6661 tccggggcca ccaacttttc cctgctgaag caggctggcg acgtcgagga gaaccctggc
     6721 cctggctccg gagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     6781 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     6841 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     6901 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     6961 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     7021 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     7081 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     7141 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     7201 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     7261 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     7321 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     7381 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtga
     7441 atctagaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     7501 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7561 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7621 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7681 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7741 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7801 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7861 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7921 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7981 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8041 cgcttccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     8101 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     8161 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     8221 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8281 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     8341 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaaggc
     8401 tagatagacc tcaggaattc gcccgaacgg gaaagctagc agaactagta ccagttgagc
     8461 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     8521 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     8581 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     8641 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8701 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8761 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     8821 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8881 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8941 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9001 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     9061 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     9121 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9181 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9241 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9301 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9361 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9421 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9481 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     9541 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9601 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     9781 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     9841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     9901 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     9961 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
    10021 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
    10081 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
    10141 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
    10201 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
    10261 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
    10321 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    10381 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    10441 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    10501 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10561 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10621 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    10681 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10741 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10801 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    10861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    11041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    11101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    12001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    12061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    12121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    12181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    12241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    12301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    12361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    12421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttacttttac
    12601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12781 ggttccgcgc acat
//
