LOCUS       sequence-402990-       10130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-FOSL2-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..46)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        218..597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        599..797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             815..995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1042..1167
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1660..1893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2078..2122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2420..2537
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2594..2612
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2636..2654
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2720..2738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2762..2780
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2813..2851
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2848..2872
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3960..4016
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4026..4733
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4168..4186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4436..4455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4756..5344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4809..4829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5227..5238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5347..5363)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5348..5364)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5915..6095
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6121..6138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6127..6351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6397..6825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6484..6503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6694..6715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6834..6854)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6839..7168
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7019..7154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7081..7100
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7216..7263
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7282..7656
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7786..7907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7823..7842)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7877..7896
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7956..7972)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7956..7972)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7969..7991)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7980..7996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8004..8010,8011..8028,8029..8034))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8049..8070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8187..8204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8358..8946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8438..8457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9117..9908,9909..9977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9740..9759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9978..10082)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
       61 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
      121 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
      181 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      241 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      301 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      361 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      421 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      481 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      541 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      601 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      661 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      721 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      781 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
      841 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      901 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      961 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1021 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1081 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1141 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1201 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1261 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1321 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1381 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1441 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1501 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1561 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1621 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1681 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1741 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1801 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1861 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1921 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1981 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     2041 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     2101 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     2161 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     2221 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     2281 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2341 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
     2401 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2461 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2521 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttagatc
     2581 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2641 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2701 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2761 ctccctatca gtgatagaga aaagtggaag tcgagctcgg tacccgggtc gaggtaggcg
     2821 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     2881 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     2941 ccccgaattc cgccaccatg taccaggatt atcccgggaa ctttgacacc tcgtcccggg
     3001 gcagcagcgg ctctcctgcg cacgccgagt cctactccag cggcggcggc ggccagcaga
     3061 aattccgggt agatatgcct ggctcaggca gtgcattcat ccccaccatc aacgccatca
     3121 cgaccagcca ggacctgcag tggatggtgc agcccacagt gatcacctcc atgtccaacc
     3181 cataccctcg ctcgcacccc tacagccccc tgccgggcct ggcctctgtc cctggacaca
     3241 tggccctccc aagacctggc gtgatcaaga ccattggcac caccgtgggc cgcaggagga
     3301 gagatgagca gctgtctcct gaagaggagg agaagcgtcg catccggcgg gagaggaaca
     3361 agctggctgc agccaagtgc cggaaccgac gccgggagct gacagagaag ctgcaggcgg
     3421 agacagagga gctggaggag gagaagtcag gcctgcagaa ggagattgct gagctgcaga
     3481 aggagaagga gaagctggag ttcatgttgg tggctcacgg cccagtgtgc aagattagcc
     3541 ccgaggagcg ccgatcgccc ccagcccctg ggctgcagcc catgcgcagt gggggtggct
     3601 cggtgggcgc tgtagtggtg aaacaggagc ccctggaaga ggacagcccc tcgtcctcgt
     3661 cggcggggct ggacaaggcc cagcgctctg tcatcaagcc catcagcatt gctgggggct
     3721 tctacggtga ggagcccctg cacaccccca tcgtggtgac ctccacacct gctgtcactc
     3781 cgggcacctc gaacctcgtc ttcacctatc ctagcgtcct ggagcaggag tcacccgcat
     3841 ctccctccga atcctgctcc aaggctcacc gcagaagcag tagcagcggg gaccaatcat
     3901 cagactcctt gaactccccc actctgctgg ctctgacgcg tgaattcgat ggatccgggg
     3961 ccaccaactt ttccctgctg aagcaggctg gcgacgtcga ggagaaccct ggccctggct
     4021 ccggagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     4081 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     4141 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     4201 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     4261 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     4321 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     4381 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     4441 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     4501 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     4561 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     4621 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     4681 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tgaatctaga
     4741 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4801 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4861 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4921 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4981 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5041 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5101 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5161 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5221 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5281 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcttcc
     5341 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5401 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5461 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5521 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     5641 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa ggctagttta
     5701 agcagatagc aacgttcgtc tgagtttgct agcagaacta gtaccagttg agcaagagaa
     5761 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5821 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5881 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     5941 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6001 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6061 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     6121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6361 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6421 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6481 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6541 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6601 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6661 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6721 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6781 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6841 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6901 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6961 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7021 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7081 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7141 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7201 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7261 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     7321 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     7381 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     7441 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     7501 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7561 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     7621 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7681 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7741 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7921 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7981 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8041 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8101 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8161 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8761 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt taccagcgtt
     9901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10081 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
//
