LOCUS       sequence-402997-       10673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 10673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-KLF4-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2613..2631,2632..2654,2655..2673,2674..2696,2697..2715,
                     2716..2738,2739..2757,2758..2780,2781..2799,2800..2822,
                     2823..2841,2842..2864,2865..2883)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2916..2954
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2951..2975
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4522..4578
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4588..5295
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4730..4748)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4998..5017
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5318..5906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5371..5391)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5789..5800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5909..5925)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5910..5926)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6477..6657
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6683..6700)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6689..6913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6959..7387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7046..7065)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7256..7277
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7396..7416)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7401..7730
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7581..7716
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7643..7662
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7778..7825
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7844..8218
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8348..8469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8385..8404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8439..8458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8518..8534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8518..8534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8531..8553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8542..8558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8566..8572,8573..8590,8591..8596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8611..8632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8749..8766)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8920..9508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9000..9019)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9679..10470,10471..10539))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10302..10321
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10540..10644)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2881 agaaaagtgg aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2941 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3001 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttccgccacc
     3061 atgaggcagc cacctggcga gtctgacatg gctgtcagcg acgcgctgct cccatctttc
     3121 tccacgttcg cgtctggccc ggcgggaagg gagaagacac tgcgtcaagc aggtgccccg
     3181 aataaccgct ggcgggagga gctctcccac atgaagcgac ttcccccagt gcttcccggc
     3241 cgcccctatg acctggcggc ggcgaccgtg gccacagacc tggagagcgg cggagccggt
     3301 gcggcttgcg gcggtagcaa cctggcgccc ctacctcgga gagagaccga ggagttcaac
     3361 gatctcctgg acctggactt tattctctcc aattcgctga cccatcctcc ggagtcagtg
     3421 gccgccaccg tgtcctcgtc agcgtcagcc tcctcttcgt cgtcgccgtc gagcagcggc
     3481 cctgccagcg cgccctccac ctgcagcttc acctatccga tccgggccgg gaacgacccg
     3541 ggcgtggcgc cgggcggcac gggcggaggc ctcctctatg gcagggagtc cgctccccct
     3601 ccgacggctc ccttcaacct ggcggacatc aacgacgtga gcccctcggg cggcttcgtg
     3661 gccgagctcc tgcggccaga attggacccg gtgtacattc cgccgcagca gccgcagccg
     3721 ccaggtggcg ggctgatggg caagttcgtg ctgaaggcgt cgctgagcgc ccctggcagc
     3781 gagtacggca gcccgtcggt catcagcgtc agcaaaggca gccctgacgg cagccacccg
     3841 gtggtggtgg cgccctacaa cggcgggccg ccgcgcacgt gccccaagat caagcaggag
     3901 gcggtctctt cgtgcaccca cttgggcgct ggaccccctc tcagcaatgg ccaccggccg
     3961 gctgcacacg acttccccct ggggcggcag ctccccagca ggactacccc gaccctgggt
     4021 cttgaggaag tgctgagcag cagggactgt caccctgccc tgccgcttcc tcccggcttc
     4081 catccccacc cggggcccaa ttacccatcc ttcctgcccg atcagatgca gccgcaagtc
     4141 ccgccgctcc attaccaaga gctcatgcca cccggttcct gcatgccaga ggagcccaag
     4201 ccaaagaggg gaagacgatc gtggccccgg aaaaggaccg ccacccacac ttgtgattac
     4261 gcgggctgcg gcaaaaccta cacaaagagt tcccatctca aggcacacct gcgaacccac
     4321 acaggtgaga aaccttacca ctgtgactgg gacggctgtg gatggaaatt cgcccgctca
     4381 gatgaactga ccaggcacta ccgtaaacac acggggcacc gcccgttcca gtgccaaaaa
     4441 tgcgaccgag cattttccag gtcggaccac ctcgccttac acatgaagag gcattttacg
     4501 cgtgaattcg atggatccgg ggccaccaac ttttccctgc tgaagcaggc tggcgacgtc
     4561 gaggagaacc ctggccctgg ctccggagtg agcaagggcg aggaggataa catggccatc
     4621 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     4681 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     4741 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     4801 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     4861 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     4921 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     4981 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     5041 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     5101 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     5161 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     5221 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     5281 gagctgtaca agtgaatcta gaacgcgtta agtcgacaat caacctctgg attacaaaat
     5341 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5401 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5461 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5521 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5581 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5641 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5701 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5761 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5821 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5881 gatctccctt tgggccgctt ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     5941 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     6001 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6061 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6121 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     6181 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     6241 atggtgctac aaggctagta agcggtctaa acgagctatt agatatgagg ctagcagaac
     6301 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     6361 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     6421 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     6481 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6541 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6601 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     6661 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6721 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6781 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6841 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6901 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7381 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7441 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7501 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7561 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7621 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7681 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7741 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     7801 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     7861 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7921 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7981 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     8041 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     8101 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     8161 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     8221 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8281 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8341 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8401 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8461 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10441 atcttttact tttaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acg
//
