LOCUS       sequence-403006-        5222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for bacterial expression of human protein ARL6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5222)
  TITLE     Kahn Lab Plasmids 2023
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET3-ARL6-His6
FEATURES             Location/Qualifiers
     source          1..5222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             750..767
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(821..839)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      835..882
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1248..1253,1254..1270,1271..1276))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1303..1321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1389..1493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1494..1562,1563..2354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1712..1731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2525..3113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3014..3033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3267..3284
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3299..3441
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3367..3386)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3527..3549
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3543..3734)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5192..5211
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
       61 atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagaaata
      121 attttgttta actttaagaa ggagatatac atatgggatt gctagacaga ctttcagtct
      181 tgcttggcct gaagaagaag gaggttcatg ttttgtgcct tgggctagat aatagtggca
      241 aaacgacgat cattaacaaa cttaaacctt caaatgctca atctcaaaat atccttccaa
      301 caataggatt cagcatagag aaattcaaat catccagttt gtcatttaca gtgtttgaca
      361 tgtcaggtca aggaagatac agaaatctct gggaacacta ttataaagaa ggccaagcta
      421 ttatttttgt cattgatagt agtgatagat taagaatggt tgtggccaaa gaagaactcg
      481 atactcttct gaatcatcca gatattaaac accgtcgaat tccaatctta ttctttgcaa
      541 ataaaatgga tcttagagat gcagtgacat ctgtaaaagt gtctcagttg ctgtgtttag
      601 agaacatcaa agataaaccc tggcatattt gtgctagtga tgccataaaa ggagaaggct
      661 tgcaagaagg tgtagactgg cttcaagaaa aaacaataca atcagatcca gactgtgaag
      721 acatgaaaag aggcagcggc agcggcagcc atcaccatca ccatcactaa ggatccggct
      781 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      841 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      901 tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag
      961 tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg
     1021 tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat
     1081 gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat
     1141 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat
     1201 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc
     1261 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
     1321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     2041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3181 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3241 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3301 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac
     3361 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     3421 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3481 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3541 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     3601 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     3661 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     3721 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     3781 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     3841 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     3901 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     3961 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     4021 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     4081 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     4141 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     4201 ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
     4261 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
     4321 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg
     4381 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
     4441 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
     4501 cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
     4561 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     4621 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     4681 ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc
     4741 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga
     4801 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
     4861 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
     4921 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     4981 tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     5041 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     5101 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     5161 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     5221 cc
//
