LOCUS       sequence-403023-       10337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fly transformation vector with Tubulin promoter and SV40 PA site and
            a UTR for ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Jenny Lab
  TITLE     Jenny Lab Wnk Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAttB-Tub
FEATURES             Location/Qualifiers
     source          1..10337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    7679..7760
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7716..7735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7770..7789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    7861..7930
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8149..8167)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8188..8204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8188..8204)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8201..8223)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8212..8228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8236..8242,8243..8260,8261..8266))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8281..8302
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8419..8436)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8590..9178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8670..8689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9349..10140,10141..10209))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9972..9991
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10210..10314)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatccggg aattgggaat tcgatatcaa
     4861 gcttgcacag gtcctgttcg ataacgtcgt actcgggaat cacctttaac ttgtccgcta
     4921 ttgggtattt gtccttaaac acattcgtaa tctctcccac aatcattgcc ctggcctctg
     4981 caatgtaaaa agtatttata aaatatcgct ggctctaaag gaaacatatt aatcaaagta
     5041 ttgaggcttt atcaatctaa catttacaag tagtcagacc acaattttta gactgcactt
     5101 ctgcgtaagt aaatgaaatg aaatggtgac ctactcttgg gtagccgtac tgttatatgc
     5161 tgacgagcct tcacctgggc ctgctttttc tcctgtttcg tctgatgcgt cggatgcggc
     5221 tggactggct gattgttggg attgggtttt ggatgctgct ccgaaaggtg ggccacagct
     5281 tttgcgatgg tggtgcccac tatgcggttg ctgcaggaac tacagctgaa tgttcgcttt
     5341 attgtctgca gctcctgggg ctggacgaag gccacgccct cacccggtgc ggccatctcc
     5401 tgcggcaaaa cgtatgtggg attcctgtcc aagtagctct cgatgcccac cataatgcgc
     5461 tccagcaggt tggccaggtg cgggtctagc tgcggcggat tcgtcgtctg gttcactgag
     5521 tttagggtct gcagaaagaa gtgtgcctcc atggtaagcg gattcatctg cacgatcagc
     5581 gggctttagt ctaaaaattc gaatgataca aactcgtact cacgtaaagc agtttgtttg
     5641 gcatcatctc ggccatcgtc ttattcttct tcgtggcctt tgttaccgga gcggctatgt
     5701 tgggcgcggt ggagcccgga agctgaaagg ttaatcgaat gcgttaactt ttctgcaact
     5761 cgaaagtttg ccgcctttgt tcgactgcca ataactgttg attcgaaaat tcgaatcgaa
     5821 gcggttgaat ttcgtagggt ggccaactac accaaagttc gccggcggtg tatgcatgtg
     5881 agtgtgagtg tgacagtggg tgtacgtgtg ctttaagcga cacaatcgca ccgcgatgta
     5941 catatttttg tagtaatgtg tgtgcatttt tcgtccggat cacggctcca taaaataaat
     6001 catatcacag ggctcaggga tttgtgagta gaagttcccg ccacttaaac gtcacttgtg
     6061 aaatcgccat cgacggtcaa cttttactca cactggtctt tcgcggacgg accgtctcaa
     6121 agtactgcct ttctgcgttg gaaaacatcg cctttttcgt ccaaaaggag tccccaggtt
     6181 cgatccgcat ggcgttgtgc gtgcgtgcct ttcttttcaa atgattacgg ctattaactt
     6241 gggggcgtta agttggaaac acgtaaattg cagactgcga ttagagtgac catgagtagg
     6301 agttcaaaat ctcctgacat cattttctta aaacctgctt tgttttttac atttctattt
     6361 aatataactc ctatttgaat aaaaaaacaa aacaagttta gatgttaaga tattaactac
     6421 atcctttgct ccaaagggag aggggaagtt atggagttaa ttaatttgct gttggaaatc
     6481 aatatggagt cagaaatata atgatttact aaaccttatt gaatcggtaa cgatgcgaat
     6541 ttatattaaa atagctttta tgaaacattc aacaaaaata ttattaatgt tggcccactt
     6601 tagcaaccgg ttaggtctac cggttgggca agcaaagatt cacgccctgg ttcgagtccc
     6661 aactagtcct gcaaaatacc gcagcaagtt ttagagagac caagtgccat tacctctccc
     6721 acttcagtta tcggttatgc ggcgtttaag tcgacagctt gccgtctcta gctccggtgc
     6781 ctatataaag cagcccgctt tccacatttc atattcgttt tacgtttgtc aagcctcata
     6841 gccggcagtt cgaacgtata cgctctctga gtcagacctc gaaatcgtag ctctacacaa
     6901 ttctgtgaat tttccttgtc gcgtgtgaaa cacttccaat aaaaactcct gcaggtgagt
     6961 actttaaaaa aaaatctagt gaaataatgc tgaaaagaaa tttgtgtggg caaaattcaa
     7021 tgggcaaaaa cgcgatgcgg ctttttctca aaatggcggc cggcctgcgt tttttcctca
     7081 aaagtgatga cgtcatgcct gttttttttt ttttgttcgc aatgaggaat ggctcttaaa
     7141 atctactaga taaaaaaaat attcattatt tctatgctgc tggaacgctt cattaatctt
     7201 aaaaattcta aattcggtta ccatgatact tcgacgcata actgtagatt ttggatagaa
     7261 ttaaagagaa aatggcgaga gagtaaaatt ccggcgtcgg caaagtagag caaaaaaatc
     7321 agtataccat ttagctacct ctctcactcg cacgcagtgc cggctcaagt tgggcgcggc
     7381 tctgcaatta tcgattttct tggggtgtgt aactaatcat ccgttttccc ttcctcctca
     7441 tccacagcgt gaaggtaccc gcccggggat cagatccgcg gccgcatagg ccactagtgg
     7501 atctggatcg cctaggcgat cctctagagt cgacctcgaa cgttaacgtt aacgtaacgt
     7561 taactcgagc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7621 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatgtcg
     7801 aggtcgacct gcagcccaag ctgctagaga tccactagtg tcgacgatgt aggtcacggt
     7861 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
     7921 ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa
     7981 cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag
     8041 cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc
     8101 gacggtcacg gcgggcatgt cgacactagt tctagccagc ttttgttccc tttagtgagg
     8161 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8341 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9001 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9961 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10321 tccccgaaaa gtgccac
//
