LOCUS       sequence-403026-        5255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for bacterial expression of human protein ARL15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5255)
  TITLE     Kahn Lab Plasmids 2023
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET3-ARL15-His6
FEATURES             Location/Qualifiers
     source          1..5255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             783..800
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(854..872)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      868..915
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1281..1286,1287..1303,1304..1309))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1336..1354)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1422..1526
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1527..1595,1596..2387)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1745..1764)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2558..3146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3047..3066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3300..3317
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3332..3474
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3400..3419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3560..3582
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3576..3767)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5225..5244
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
       61 atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagaaata
      121 attttgttta actttaagaa ggagatatac atatgtctga tctccgaata actgaggcgt
      181 ttctgtacat ggattatctg tgttttagag cactttgctg caagggacca ccacctgcac
      241 gaccagaata tgacctggtt tgcataggcc tcacaggttc tggcaaaacc agtctgttgt
      301 ccaaactctg cagtgaaagc cccgataacg tcgtgtcgac cacaggtttt agtattaaag
      361 cagtgccatt ccagaatgcc atcttgaatg taaaagaact tggaggggct gataacatcc
      421 ggaaatactg gagccgctac taccaaggat ctcaaggggt aatatttgta ttagacagtg
      481 cctcttcaga ggatgattta gaagctgcta gaaatgagct gcactcagct cttcagcatc
      541 cacagttatg cactttaccc tttttaatat tggccaatca tcaagacaag ccagcagctc
      601 gctcagtaca agagatcaaa aaatattttg aacttgaacc acttgcacgt ggaaaacgct
      661 ggattctaca gccctgctca ctggatgaca tggatgcact gaaagacagc ttctctcagc
      721 tgattaattt gttagaagaa aaagaccatg aagctgtaag aatgggcagc ggcagcggca
      781 gccatcacca tcaccatcac taaggatccg gctgctaaca aagcccgaaa ggaagctgag
      841 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      901 ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt
      961 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
     1021 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
     1081 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa
     1141 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     1201 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
     1261 taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt
     1321 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1381 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1441 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1501 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1561 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1621 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1681 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1741 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     1801 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1861 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1921 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1981 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2041 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     2101 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2161 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2221 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2281 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2341 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2401 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2461 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2521 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2581 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2641 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2701 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2761 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2821 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2881 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2941 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3001 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3061 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3121 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3181 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3241 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3301 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     3361 tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc
     3421 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     3481 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3541 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3601 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     3661 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     3721 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     3781 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     3841 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     3901 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     3961 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     4021 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     4081 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     4141 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     4201 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg
     4261 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     4321 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     4381 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
     4441 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     4501 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta
     4561 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     4621 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     4681 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     4741 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     4801 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     4861 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     4921 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
     4981 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
     5041 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     5101 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     5161 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     5221 catcggtgat gtcggcgata taggcgccag caacc
//
