LOCUS       sequence-403029-        5145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene 
            upstream of sfGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1791 to 1825)
  AUTHORS   Lugagne JB, Blassick CM, Dunlop MJ
  TITLE     Deep model predictive control of gene expression in thousands of 
            single cells.
  JOURNAL   Nat Commun. 2024 Mar 8;15(1):2148. doi: 10.1038/s41467-024-46361-1.
  PUBMED    38459057
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-03-8"; volume: "15(1):2148. doi"; pages: " 
            10.1038/s41467-024-46361-1"
COMMENT     Sequence Label: pCB11
FEATURES             Location/Qualifiers
     source          1..5145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(133..721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(213..232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(809..903)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(924..1583)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1498..1517
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1752..1770
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1791..1825
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      2585..2656
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2672..2699
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3013..4203
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(3365..3384)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     RBS             4204..4215
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4222..4935
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      4962..5033
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5049..5076
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
       61 cgcatgcaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      121 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      181 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      241 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      301 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      361 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      481 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      541 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
      601 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      661 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      721 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      781 agggattttg gtcatgacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
      841 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      901 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
      961 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     1021 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     1081 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     1141 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     1201 ccataacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     1261 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     1321 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     1381 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     1441 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     1501 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     1561 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     1621 aaaaatacga ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgggc
     1681 cggccaacgt ctcattttcg ccagatatcg acgtctaaga aaccattatt atcatgacat
     1741 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgag ttgacggcta
     1801 gctcagtcct aggtacagtg ctagctgtta aacgctcctc gtaagaacac gggaaagcaa
     1861 tgagaattct tttagtggag gatgatttgc cgctggcgga aacccttgct gaagcattga
     1921 gtgaccagct ttacaccgtt gatattgcca ccgacgcttc cctcgcctgg gactatgcct
     1981 cccgactgga atatgacctc gttattttgg atgtgatgct gccggagttg gacgggatta
     2041 ccctctgtca aaaatggcga tcgcacagtt atttaatgcc aattttgatg atgacagcca
     2101 gggatacgat caatgataaa atcacgggct tggatgcggg ggcggatgat tatgtggtca
     2161 agccagtgga tttgggggag ttatttgcca gggtgcgagc tttgttgcgt cggggttgtg
     2221 caacgtgcca accagtttta gagtgggggc caatcaggtt ggatccaagc acctatgaag
     2281 ttagttatga caatgaggtt ttgtctttga cccgcaagga atacagcatt ctggaattac
     2341 tactccgcaa tggccgtcgg gtgctaagtc ggagcatgat tatcgatagt atctggaagt
     2401 tggagagtcc cccagaggaa gatacggtta aggtgcatgt gcggagtttg cgacaaaaat
     2461 taaaaagtgc cggtttatca gcagatgcca ttgaaacggt ccatggcatt gggtatcgtc
     2521 tggccaattt aacggaaaaa tctttgtgcc aagggaaaaa ctagtaataa tctagaccag
     2581 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2641 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2701 ttatagtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt
     2761 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg gttagctagt
     2821 caagcccatt gtgcttttct ctatcaacct cagcttacct gaaggggtga acaggtctgg
     2881 gttaattcat gttgcgaaat gtaacagttt tagtcgcatc agctaacttt ccgatttctt
     2941 tacgattttc tccccctttt cttcaatttt actttgttag gatcgcattt ttaacgcagt
     3001 caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg
     3061 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc
     3121 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa
     3181 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg
     3241 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg
     3301 tggatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     3361 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     3421 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     3481 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     3541 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc
     3601 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     3661 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     3721 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     3781 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     3841 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     3901 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     3961 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     4021 caaggatcgc tcgcggctct taccagccta acttcgatca ttggaccgct gatcgtcacg
     4081 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc
     4141 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc
     4201 tgaaaagagg agaaatacta gatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct
     4261 attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt
     4321 gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg
     4381 ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt
     4441 tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg
     4501 caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa
     4561 tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac
     4621 ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc
     4681 gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat
     4741 ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt
     4801 ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag
     4861 aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg
     4921 gatgaactgt acaaatgatg ataataatct agaccaggca tcaaataaaa cgaaaggctc
     4981 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     5041 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtacccg tggatcctct
     5101 agagtcgacc tgcaggcatg caagcttcct aggcgttcgg ctgcg
//
