LOCUS       sequence-403126-       11544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P4 cosmid with CRISPR-Cas9 system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11544)
  AUTHORS   Fa-Arun J, Huan YW, Darmon E, Wang B
  TITLE     Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into 
            Multiple Gut Pathogens.
  JOURNAL   ACS Synth Biol. 2023 Feb 17;12(2):596-607. doi: 
            10.1021/acssynbio.2c00615. Epub 2023 Feb 2.
  PUBMED    36731126
REFERENCE   2  (bases 1 to 11544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00615"; journalName: "ACS Synth Biol"; date: 
            "2023-02-17- 17"; volume: "12"; issue: "2"; pages: "596-607"
COMMENT     Sequence Label: P4 cosmid
FEATURES             Location/Qualifiers
     source          1..11544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(154..232)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             528..4634
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             528..4631
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4655..4786
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   4787..4822
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4853..4888
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     terminator      complement(4929..5023)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      5130..5901
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             5902..6564
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            6575..6683
                     /note="incP origin of transfer"
     CDS             complement(6854..7513)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7428..7447
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     8001..8020
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8214..8236
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8228..8245
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8229..8245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11119..11136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      11140..11187
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(11222..11238)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11222..11238)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11235..11257)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11246..11262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11270..11276,11277..11294,11295..11300))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11315..11336
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11453..11470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc
       61 cgtcaagttg tcataattgg taacgaatca gacaattgac ggctcgatgg agtagcatag
      121 agaagtcaga gtagaataga agtatcaaaa aaagcaccga ctcggtgcca ctttttcaag
      181 ttgataacgg actagcctta ttttaacttg ctatgctgtt ttgaatggtt ccaacaagat
      241 tattttataa cttttataac aaataatcaa ggagaaattc aaagaaattt atcagccata
      301 aaacaatact taatactata gaatgataac aaaataaact actttttaaa agaattttgt
      361 gttataatct atttattatt aagtattggg taatattttt tgaagagata ttttgaaaaa
      421 gaaaaattaa agcatattaa actaatttcg gaggtcatta aaactattat tgaaatcatc
      481 aaactcatta tggatttaat ttaaactttt tattttagga ggcaaaaatg gataagaaat
      541 actcaatagg cttagatatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat
      601 ataaggttcc gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa
      661 aaaatcttat aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca
      721 aacggacagc tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga
      781 ttttttcaaa tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt
      841 ttttggtgga agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg
      901 aagttgctta tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt
      961 ctactgataa agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc
     1021 gtggtcattt tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat
     1081 ttatccagtt ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg
     1141 gagtagatgc taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc
     1201 tcattgctca gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt
     1261 cattgggttt gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac
     1321 agctttcaaa agatacttac gatgatgatt tagataattt attggcgcaa attggagatc
     1381 aatatgctga tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata
     1441 tcctaagagt aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct
     1501 acgatgaaca tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag
     1561 aaaagtataa agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg
     1621 ggggagctag ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg
     1681 gtactgagga attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct
     1741 ttgacaacgg ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa
     1801 gacaagaaga cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga
     1861 cttttcgaat tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga
     1921 tgactcggaa gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag
     1981 gtgcttcagc tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg
     2041 aaaaagtact accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga
     2101 caaaggtcaa atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga
     2161 agaaagccat tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa
     2221 aagaagatta tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag
     2281 atagatttaa tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag
     2341 attttttgga taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct
     2401 tatttgaaga tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg
     2461 ataaggtgat gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa
     2521 aattgattaa tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat
     2581 cagatggttt tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta
     2641 aagaagacat tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg
     2701 caaatttagc tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg
     2761 atgaattggt caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac
     2821 gtgaaaatca gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg
     2881 aagaaggtat caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc
     2941 aattgcaaaa tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg
     3001 accaagaatt agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa
     3061 gtttccttaa agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg
     3121 gtaaatcgga taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac
     3181 aacttctaaa cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac
     3241 gtggaggttt gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc
     3301 gccaaatcac taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg
     3361 aaaatgataa acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg
     3421 acttccgaaa agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc
     3481 atgatgcgta tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg
     3541 aatcggagtt tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt
     3601 ctgagcaaga aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact
     3661 tcttcaaaac agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa
     3721 ctaatgggga aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca
     3781 aagtattgtc catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat
     3841 tctccaagga gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag
     3901 actgggatcc aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag
     3961 tggttgctaa ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag
     4021 ggatcacaat tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta
     4081 aaggatataa ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg
     4141 agttagaaaa cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg
     4201 agctggctct gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt
     4261 tgaagggtag tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt
     4321 atttagatga gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg
     4381 ccaatttaga taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac
     4441 aagcagaaaa tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta
     4501 aatattttga tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg
     4561 ccactcttat ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc
     4621 taggaggtga ctgaagtata ttttagatga agattatttc ttaataacta aaaatatggt
     4681 ataatactct taataaatgc agtaatacag gggcttttca agactgaagt ctagctgaga
     4741 caaatagtgc gattacgaaa ttttttagac aaaaatagtc tacgaggttt tagagctatg
     4801 ctgttttgaa tggtcccaaa actgagacca gtctcggaag ctcaaaggtc tcgttttaga
     4861 gctatgctgt tttgaatggt cccaaaactt cagcacactg agacttgttg agtggatccg
     4921 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     4981 agatggagtt ctgaggtcat tactggatct atcaacagga gtcctagaca gctgggcgcg
     5041 ccccccctac gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc tcccttgcca
     5101 gcccgtggat atgtggacga tggccgcgag cggccaccgg ctggctcgct tcgctcggcc
     5161 cgtggacaac cctgctggac aagctgatgg acaggctgcg cctgcccacg agcttgacca
     5221 cagggattgc ccaccggcta cccagccttc gaccacatac ccaccggctc caactgcgcg
     5281 gcctgcggcc ttgccccatc aattttttta attttctctg gggaaaagcc tccggcctgc
     5341 ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa ggctcgcgca
     5401 gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga cccaagccta tgcgagtggg
     5461 ggcagtcgaa gggcgaagcc cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg
     5521 ggttccaagg gggcagcgcc accttgggca aggccgaagg ccgcgcagtc gatcaacaag
     5581 ccccggaggg gccacttttt gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg
     5641 caggggtgcc cttctttggg caccaaagaa ctagatatag ggcgaaatgc gaaagactta
     5701 aaaatcaaca acttaaaaaa ggggggtacg caacagctca ttgcggcacc ccccgcaata
     5761 gctcattgcg taggttaaag aaaatctgta attgactgcc acttttacgc aacgcataat
     5821 tgttgtcgcg ctgccgaaaa gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac
     5881 ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat tcaagccaag
     5941 aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg ggccgggctt
     6001 attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca gatgggccac
     6061 cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg ttctttgcgg
     6121 acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt cgtgaagctc
     6181 aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg gggccagccc
     6241 cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca cgacgaccag
     6301 gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta tccgggcgag
     6361 cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat tccgggcatg
     6421 gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg gcagcagcgc
     6481 ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga gccgccgaca
     6541 cgggtaacgc tgccgcgccg gtagggccgg cctacggcca gcctcgcaga gcaggattcc
     6601 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
     6661 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
     6721 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
     6781 ctataatgac cccgaagcag ggttatgcag cggaaaagga caaaagtcaa atgcaagcga
     6841 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     6901 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     6961 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     7021 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     7081 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     7141 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     7201 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     7261 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     7321 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     7381 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     7441 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     7501 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     7561 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaaag
     7621 atccggatca accactttta caagaaagct gggtccgaag gtgaacaatc cactgttcac
     7681 ccttcaccgt atattcaccc gttatcacac tgaaattaaa agagaaaaac gaaaggtgaa
     7741 cagtgtgaac aatcaaatca aaaaaaactt ttttcttcct gagtgatttc agtgcggagg
     7801 attaatcacc ggtatgagtc acatcggcag aatgccggag gtgaagaatc gaatgttcac
     7861 ccttcaccca ttattcacca cctatcactc tgaaataaaa ggagaaaaca gaaaggtgaa
     7921 cagtgtgaac agttctttcg aaaaaaaatt ttttggcgcg tcagcgggtg ttggcgggtg
     7981 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     8041 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     8101 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     8161 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     8221 acgacgttgt aaaacgacgg ccagtgaaat tattaaccta ggctgacttc atccgcacac
     8281 acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa
     8341 gaattcttaa ggatcttgcg acactgacaa gaaccacctt acatcaatct attttttgca
     8401 tatttttcag gatagtgcca tcaaaaaaga cagttttgga ccataggcca tagattttct
     8461 tcattatgct ccaatgtatt aacgaggtat gtactgaagg cactttgact acacaaataa
     8521 ttaactaact tgtcggtttg cgcgcggtgc tttccccgcc tcgcccgccc gctttgcggg
     8581 gcggttttaa tgcagttgca ctgacacgct cagaccgcgc cgggaatggc gcggtttgca
     8641 gaaaatgagg caggaaaacg catgcaaagc catgcacctt atcgatgcat ggcttttttc
     8701 agtaaaaacg ggcggatttt cggggaattt tacactgact gacgtgatgc cagttgcgca
     8761 cttttacgcg aaaaaatcat gttctgcgca ggggtgaatt tttcacggct gtcatccacc
     8821 gaagccacgt caggcctgaa tccgatggtc gttaaaatgt cgctatcctg tgcggaataa
     8881 ttaatttttt cacccttcgc aagccaggac agaagggctt cacgcagggc atctgtggca
     8941 cgctgtatgg cacagtttcg ggcaatggcc gtcagctcac tgtagcccat cagctccggt
     9001 gccagtgcgg ccgccagtgc tgtgccgtgc tgctgcataa aatcattcag ccggtcgcgg
     9061 atgctgatgt gctgaacggc ttcatgcgaa cgaatataac gaccggcggc ctgattcacc
     9121 tgccattttc tgacttcgat aatattgcgt aattcgtcca ggcgactgac gtttctgcct
     9181 tctcctgaca gaagccgcag atattcctgt tcggccgctg ccagctcatt tttgcgttgc
     9241 agccatgctg ctttgttatt ctgacaggtg tcaaaggcct gctgtaaggc tgtgctttcc
     9301 atcgttatct ctttctcatc atgctgaaga ataaaaatac ggtgtgcggc gacggccggt
     9361 gttaaccggc agccctcatt ccagacgcag cgaatatgat tgtgttttta accgtactgg
     9421 cggcagttcc tgtttttcat gcaggcgttc tgccagttcg tccggcgtga ccgggcggac
     9481 aatgaagcgg ttgatggtct gaagcgtttt aaacaccaga ccacagcccg gatccgtgca
     9541 cacataaaaa cgctcggtga cttcctgaga cagacgccgc gatgttcttg acagtgcaag
     9601 gcctttacat ctccgacaac aatatccggt aacaagcatt cttttcgggc gtttcatacc
     9661 gcaggaggct gacgtcagtg aatcgcggta tctctgtttg cctgaaatgt attccattcc
     9721 tgaatcttta ctgtcagaga aaaagctttc actcgcttca aatgtcgcag agcaataaat
     9781 attccggcac tgtgcaatca ttatcttggt gccatcgtcc atgaaatgtg cgcgacgggt
     9841 gtgagcaaca tgtccacacg acggacagta aatcatgaca gcagccctct ggccttaagc
     9901 tctgctccct gctggtctat tttgtcctgc cacaccttgc gctgtgccgg tgtgcctgcc
     9961 acctcataat ccatgtgcgg gagtgttgcc gctgacagtc cggtcagccg gagaaccggc
    10021 tcgccggtga ggctgatttg catctgttta attttctgtt ccagcgatga tttcacctgc
    10081 tgcatgacag ccttttccgg tgcgacgtag ccctgatggc cggtggtgtt ggcgagcgga
    10141 ttttcctgta ccagaatgct cagatgcatt gcccggacaa gcgcctcaca ggtttcattc
    10201 agggcgtgtt ccagctcatg ctctgcatac agactcagaa ggtgatgatg tgccttccgg
    10261 taggcggtgg ccgtgctgtc acacgcccct ttcaggcgtt cacgttcgaa attcagcacc
    10321 acggccagat tgtcatattc ctgtaccagc tcccggcgtg ccacgcgctc aatgtggcgc
    10381 tgtttcagct cgtcgctcag gacaccaccg gctgcacgaa aggccgtgcg ccagtcgtca
    10441 gcgtcgtttc cgtcggcctg cgccagcgca tttttttcct gctctgcccg ttcaatggcc
    10501 gtgacagtct catccatcag gcgggcgttc tcaagatggg cggctctggc cttttccagt
    10561 tgtgccagtg cgggttgcag atattcaggg atagtgtggt cagacatttt ccggctcctc
    10621 gtcacttcag gttaagaaaa ttgtgacgta caccggacaa caacacgacg cattgcagat
    10681 gtgccagccc tgacacagga gactcatcct cagaccggca agccaggaaa aggtcgcagg
    10741 aaaaaccggc ttactgtttg tttttttata ttttactgtt cacctctgtt caccttaata
    10801 aaaaagataa gtaatacagt aagttaaagg gtgaacaatc gcagtaatga ctgttcaccg
    10861 tctgttcacc actgttcacc cgttgatggg ctttttgtgc tgtttactac tgtttgtttt
    10921 tattaattcg ccgggaatga ataagaagaa acgatttgta tttcactata aaaattacaa
    10981 attgctttaa gtggctttaa atgactttaa gtggagatga acaaaaaaca cacagccatt
    11041 gtaagacagc ctgaacaaat cccccctgtt gcgtctgctg aaaatattca caaaataaag
    11101 cgctcagcac aggcagcaca tcatcatcac caccactaac tagcataacc ccttggggcc
    11161 tctaaacggg tcttgagggg ttttttgatc ccaatggcgc gccgagcttg gctcgagcat
    11221 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    11281 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    11341 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    11401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    11461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    11521 taatacggtt atccacagaa tcag
//
