LOCUS       sequence-403135-       17008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fly transformation vector expressing indicated Wnk variant under 
            tubulin promoter for ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Jenny Lab
  TITLE     Jenny Lab Wnk Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 17008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAttB-Tub-HA-WnkDeltaCC1
FEATURES             Location/Qualifiers
     source          1..17008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    156..237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(193..212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     247..266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    338..407
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(626..644)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(665..681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(665..681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(678..700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    689..705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(713..719,720..737,738..743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    758..779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(896..913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1067..1655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1147..1166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1826..2617,2618..2686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2449..2468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2687..2791)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2817..3272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2954..2975)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3166..3185
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3399..3421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3413..3430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3414..3430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3437..3456
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3437..3455
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3489..3507)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3959..4030,4432..4705,4780..5434,5496..5811,6015..6146,
                     6217..6831)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7606..7639
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             10306..10332
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagtcga cctcgaacgt taacgttaac gtaacgttaa ctcgagctag atcataatca
       61 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      241 ctagttgtgg tttgtccaaa ctcatcaatg tatgtcgagg tcgacctgca gcccaagctg
      301 ctagagatcc actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
      361 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
      421 tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct
      481 cgccctcgaa accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg
      541 cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga
      601 cactagttct agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
      661 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      721 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      781 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      841 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     2821 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2881 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2941 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3001 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3061 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3121 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3181 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3241 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     3301 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3361 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     3421 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacgt accgggcccc
     3481 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt
     3541 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat
     3601 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac
     3661 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc
     3721 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct
     3781 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta
     3841 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga
     3901 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat
     3961 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca
     4021 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact
     4081 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc
     4141 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
     4201 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
     4261 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
     4321 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
     4381 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc
     4441 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg
     4501 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac
     4561 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga
     4621 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg
     4681 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga
     4741 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg
     4801 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc
     4861 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg
     4921 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc
     4981 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc
     5041 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt
     5101 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg
     5161 gcggagaaag gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga
     5221 tctgcgatga gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc
     5281 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg
     5341 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg
     5401 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt
     5461 atctagtatt acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac
     5521 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag
     5581 tcccgtgatc ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat
     5641 atggagcagt tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg
     5701 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg
     5761 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt
     5821 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta
     5881 gatagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata
     5941 gttttgcaga agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac
     6001 attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca
     6061 cgcaagtggg tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct
     6121 ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat
     6181 aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt
     6241 tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg
     6301 attgccgaat tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg
     6361 atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg
     6421 gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg
     6481 atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc
     6541 ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt
     6601 tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc
     6661 tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac
     6721 ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc
     6781 cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat
     6841 atccgaaata actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat
     6901 tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat
     6961 agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa
     7021 ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac
     7081 accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc
     7141 tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac
     7201 attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg
     7261 ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat
     7321 gtacaattta tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta
     7381 atttaatatt atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta
     7441 caatttgaac tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa
     7501 tggtgggcat aatagtgttg tttatatata tcaaaaataa caactataat aataagaata
     7561 catttaattt agaaaatgct tggatttcac tggaactagg ctagcataac ttcgtataat
     7621 gtatgctata cgaagttatg ctagcggatc cgggaattgg gaattcgata tcaagcttgc
     7681 acaggtcctg ttcgataacg tcgtactcgg gaatcacctt taacttgtcc gctattgggt
     7741 atttgtcctt aaacacattc gtaatctctc ccacaatcat tgccctggcc tctgcaatgt
     7801 aaaaagtatt tataaaatat cgctggctct aaaggaaaca tattaatcaa agtattgagg
     7861 ctttatcaat ctaacattta caagtagtca gaccacaatt tttagactgc acttctgcgt
     7921 aagtaaatga aatgaaatgg tgacctactc ttgggtagcc gtactgttat atgctgacga
     7981 gccttcacct gggcctgctt tttctcctgt ttcgtctgat gcgtcggatg cggctggact
     8041 ggctgattgt tgggattggg ttttggatgc tgctccgaaa ggtgggccac agcttttgcg
     8101 atggtggtgc ccactatgcg gttgctgcag gaactacagc tgaatgttcg ctttattgtc
     8161 tgcagctcct ggggctggac gaaggccacg ccctcacccg gtgcggccat ctcctgcggc
     8221 aaaacgtatg tgggattcct gtccaagtag ctctcgatgc ccaccataat gcgctccagc
     8281 aggttggcca ggtgcgggtc tagctgcggc ggattcgtcg tctggttcac tgagtttagg
     8341 gtctgcagaa agaagtgtgc ctccatggta agcggattca tctgcacgat cagcgggctt
     8401 tagtctaaaa attcgaatga tacaaactcg tactcacgta aagcagtttg tttggcatca
     8461 tctcggccat cgtcttattc ttcttcgtgg cctttgttac cggagcggct atgttgggcg
     8521 cggtggagcc cggaagctga aaggttaatc gaatgcgtta acttttctgc aactcgaaag
     8581 tttgccgcct ttgttcgact gccaataact gttgattcga aaattcgaat cgaagcggtt
     8641 gaatttcgta gggtggccaa ctacaccaaa gttcgccggc ggtgtatgca tgtgagtgtg
     8701 agtgtgacag tgggtgtacg tgtgctttaa gcgacacaat cgcaccgcga tgtacatatt
     8761 tttgtagtaa tgtgtgtgca tttttcgtcc ggatcacggc tccataaaat aaatcatatc
     8821 acagggctca gggatttgtg agtagaagtt cccgccactt aaacgtcact tgtgaaatcg
     8881 ccatcgacgg tcaactttta ctcacactgg tctttcgcgg acggaccgtc tcaaagtact
     8941 gcctttctgc gttggaaaac atcgcctttt tcgtccaaaa ggagtcccca ggttcgatcc
     9001 gcatggcgtt gtgcgtgcgt gcctttcttt tcaaatgatt acggctatta acttgggggc
     9061 gttaagttgg aaacacgtaa attgcagact gcgattagag tgaccatgag taggagttca
     9121 aaatctcctg acatcatttt cttaaaacct gctttgtttt ttacatttct atttaatata
     9181 actcctattt gaataaaaaa acaaaacaag tttagatgtt aagatattaa ctacatcctt
     9241 tgctccaaag ggagagggga agttatggag ttaattaatt tgctgttgga aatcaatatg
     9301 gagtcagaaa tataatgatt tactaaacct tattgaatcg gtaacgatgc gaatttatat
     9361 taaaatagct tttatgaaac attcaacaaa aatattatta atgttggccc actttagcaa
     9421 ccggttaggt ctaccggttg ggcaagcaaa gattcacgcc ctggttcgag tcccaactag
     9481 tcctgcaaaa taccgcagca agttttagag agaccaagtg ccattacctc tcccacttca
     9541 gttatcggtt atgcggcgtt taagtcgaca gcttgccgtc tctagctccg gtgcctatat
     9601 aaagcagccc gctttccaca tttcatattc gttttacgtt tgtcaagcct catagccggc
     9661 agttcgaacg tatacgctct ctgagtcaga cctcgaaatc gtagctctac acaattctgt
     9721 gaattttcct tgtcgcgtgt gaaacacttc caataaaaac tcctgcaggt gagtacttta
     9781 aaaaaaaatc tagtgaaata atgctgaaaa gaaatttgtg tgggcaaaat tcaatgggca
     9841 aaaacgcgat gcggcttttt ctcaaaatgg cggccggcct gcgttttttc ctcaaaagtg
     9901 atgacgtcat gcctgttttt ttttttngtt cgcaatgagg aatggctctt aaaatctact
     9961 agataaaaaa aatattcatt atttctatgc tgctggaacg cttcattaat cttaaaaatt
    10021 ctaaattcgg ttaccatgat acttcgacgc ataactgtag attttggata gaattaaaga
    10081 gaaaatggcg agagagtaaa attccggcgt cggcaaagta gagcaaaaaa atcagtatac
    10141 catttagcta cctctctcac tcgcacgcag tgccggctca agttgggcgc ggctctgcaa
    10201 ttatcgattt tcttggggtg tgtaactaat catccgtttt cccttcctcc tcatccacag
    10261 cgtgaaggta cccgcccggg gatcagatcc gcggccgcca ccatgtaccc ctacgacgtg
    10321 cccgactacg cgatggatag caaaatagaa cctgcagatg ccataagcaa ccagaaagct
    10381 ggtcttactt cgggcaaacc taaaaaagac aagtcagtca aggggacgtc gcaagcggtt
    10441 gtttcagaca ccaagaaaat ggaggccaga aagtcctcct cggacacggt gatcgagcca
    10501 ttgatcaagc agcaggcgcc actgcactcg tcaaagacca cgccaacgac tttgacggcg
    10561 aacttcgtgc aaaacattcg attcgtccgt aaaaatgtgg aacagtcagg caggaacacc
    10621 aatccactgc agttcgttga actcgagaca gagtttcctc gtgactatga cgacaacatc
    10681 gagatgcttt cccgcgaggc ggagcatctg gaggagcagt tccgcacgcc tacgcgctcc
    10741 aatgctacgg atgccacttc ccaccatgtg gccggaatca tcgacaacat tataaccgaa
    10801 gcgtcacgga gtcttacaat cgaaaagact gaagacgatg tgccattgaa gtcttctacc
    10861 aagcactcaa gcggcgtaaa acgagttggc tttcaagtag aggataagga cgataccgaa
    10921 gttcagagcg aaaagcaggc aaagagcttc gacgacataa ccaaaaaagc ggagagctcc
    10981 gaggctagcg cagaggaagc tgctgtcacc gggtcctcga cagatgcatc tgcatctccc
    11041 ctgccctcca cgtcgctggt ctccaccaca tcttcagcta catcgataac caagagtaag
    11101 agcgatgagg acgatgatcc ggtggccatg tcgccgtgcg gacgattctt taagtacgat
    11161 aaggaagtcg gacgcggttc ctttaaaact gtctatcgtg gcctggacac gttgactggt
    11221 gttccagttg cctggtgcga gttattggac aagcaagtaa aaaaaagtga acgtactaga
    11281 tttcgcgagg aggcagacat gctgaagaaa cttcagcacc caaacatagt gcggttttat
    11341 acatattggg agtttcctat tggccgcaag aagaatattg tactagtcac cgaactaatg
    11401 ttatctggaa ctttgaaatc ctatttgaaa cgattcaaaa aaatacaccc aaaggtattg
    11461 aagtcatggt gtcgccagat actaaaaggc cttaacttcc tgcatactcg tcaatttcct
    11521 ataattcatc gtgatttgaa atgtgataat atttttataa ctggcaccac tggtagtgtt
    11581 aaaatcggcg acttgggcct ggcaactttg aaaaaccgct cccacgcgaa atctgtgatt
    11641 ggtacgccgg agtttatggc cccggaaatg tacgaggagc actacgacga gtcggtggac
    11701 gtatatgcat tcggcatgtg catgttggag atggccattt ccgaatatcc ttacagtgag
    11761 tgcaagggtc ctgcacagat ctacaaaaag gtgatatcgg gcattaagcc agcggcgttg
    11821 gctaaggtgg aggatcccaa cgtgcgcgac attatcgaac ggtgcattga acttaaaaag
    11881 gaggatcgcc ccagctgcaa tgaactgcta gaatcggagt tctttgacga agatatcggt
    11941 atacgagtgg agccaactgc ttcggagcag ttcctttctg atccgagcat cagcatcatc
    12001 gagttccgac tgcggtttat ggatcccaag aagcgctcgt cccgtcataa ggaaaacgag
    12061 gcaattcagt ttgagtacaa catcaggcat gacgagtacg aacagattgc gcaagaaatg
    12121 atgaaggaaa atattatatc tgaggacgac tctcgcgctg tagctcggct tttaaaagtg
    12181 caggtggtgt cgttgcttag tttgccgact aacgtcgatg aagacgagga agaggaggag
    12241 gagtccgagg acgaggagga tggtgttaaa tggaatcagc gcctgcagct aagatatgac
    12301 ctgcttaaca ctgattcaga aacaagcctg gctctatcca cgaacagcgt cgaaccgcag
    12361 cagctgtcca ctcgctcgaa cacttcgatt ccgaacagtg gcattcagca acctgttcag
    12421 gtcccaggtc aagtgcctgt ttcccagttg atctcggtgc aaccacaggc tattccgtca
    12481 ccggctattc ccatgcaaca gaagccgacg gtgcactaca ttcaaccgcc acagctggcc
    12541 tcttatcaaa attcgaatgc atcgatgcag gagatgacca ataaccaggt catttcgccg
    12601 acaggcagtc aacaaatgca acagcaacaa cccgtagtgg caccaacagt caatcatcag
    12661 gtcatgccgc agcagcaagt gaaccaacaa cagcaacaac cacaaatgat gcagcaaata
    12721 ccccagcaag ttcaagtgca acagccccaa actgtattgc ctccgcagcc acatgagcag
    12781 cagccacagc aacaacaaca gccactgcag cagcaactgc agcagcttat gcatactaac
    12841 gtgcaggctc cggatttgac tcaacagcaa caaatggctc agcaacaggc ccagcaatat
    12901 tttcagcagc agcagcaaca gcctcagcag gccgttaaca tgcaacaagc ttatgcaatg
    12961 cagcaagctg gccaacagca acagctttcg cagcctcttc aaattcagca acagatcttg
    13021 cagcaacaac aagtggccgt ttcacatcag caacagataa tgcagcaaca actggcccag
    13081 catcagctgc aacagctgca gcagcagcag cttcaacaac agcaactgca acagcaacag
    13141 cagcagcaaa tacaacaaca gcaactccaa caacaacaac tccagcaaca gcaattcgta
    13201 cagcagtatg cgcaggcaat gcctcagcaa caacatcagc aactcgttac gggatcgcaa
    13261 gtcatggcac cgcatcaaca tcagcaacct attcagatcc cagtgcagat gcaagttcca
    13321 ccgacgtccg tggctcctcc aatacaacac acttacaatc agcagggcgg acaagtaacg
    13381 ctcagtgatg cccaacagca acagcatcct ggattctcgg ccgtaccaca acaggcagcg
    13441 ccatttatac aacagcccac acagcaaccc attcagctat caatgccttt ggaacaacaa
    13501 ttgcagcaat tgctgcatag ccagccagcc cagcaacaac aggccatgag ccaacagcag
    13561 caacagtcta tggtccaaca gcagcaacag cccttggtgc aacagcaaca actgcctttg
    13621 gtacaacagc agccgccttt ggtccagcag cagcaaccgc tggttcagca tcagcagcct
    13681 tcggtccaac atcagcaacc tttggtgcaa cagccccagc aacagcaacc acagccccag
    13741 aatcagcaac cacagcccca gacacagcaa acacacgtgg ttcagcaaca accaccacag
    13801 cagcaacctg cagtagagca aatctcacag atttcttcgc aagtgcctgt tcaacaggaa
    13861 aatctgcagc caatacaggt caacaaagat gcgaatgtcg caacggatgc aatgtcccta
    13921 aatagcgccc acggtgccct cgaacctgca ccgaagaccg aaccccagaa ctctgctgat
    13981 gcggaaaagc agcaaaagca gacaggcacg ggcacacgtt cccagaaacc gaggcgatca
    14041 aaccggagcg gcaacgaacg gattcccaaa cttagtgtaa ccagcgtcga cgagggcagc
    14101 gtcataaact gtcacatgga aaataaactt aaaacgatta catttaaatt cgacatagga
    14161 gacgtaaatc cggttgaaat agccaataaa ttgatcgccc aagatttgct ttctaattgt
    14221 caaagtacag tttttgtgga aatgatcaat gaaattgttg accaagtcaa acagaatccc
    14281 aatcaaatcc ctataccgac aaattatcgc cgcaatattg aaaagcggga tgaaaccgcc
    14341 agcgacatca caaagatgtt cgagcccact atccatggtg ttgaaaccct gccatccggt
    14401 ggaggaggcg ctgaacagtc aaactgcaac ttgactttag aggccaggtc ccatttatcc
    14461 aacataccaa atgcgaaaga accacagctg aacgtcagca ctccgcccac aaccacttcg
    14521 actatgtctt cctcatcaac cgcatcgagg gatgcgccca atagtagcaa tgacgtgacc
    14581 atcggctctg gttccgtttc tcgaaaaacg agcacggcct cggagtacac ttcgctatcg
    14641 attgactata tgcccgacag caacattact ccaactggtc cggagccacc gcttgacgat
    14701 ggcaaggata aaaaaccttg ctcccttgct atagccagaa tgcaaaagct cttggagtcg
    14761 gccggcaatg gtgctcctcg cagcctgaat ttgcccttga atcgtcacct gaaaattcaa
    14821 gaggacttga agcatactag aagtcttgac gatttgaccg cagtgaaaat aacctttgac
    14881 atgcccaaca aggctgcatt ggacacttcg gaaaacgctc agcaggccac agaagcggaa
    14941 gcggagaaac caaaagataa atccggacag gcagtaggca accagggaac tggagcggcc
    15001 aacactctag agcagttaaa aatagaactg gagaacatta ctcatgccca cgcgtttgcc
    15061 tccgctgtag tggcctcgat taacaacaga gctcctcatc aagcttcccc agcaatgtcc
    15121 tccctaaagg ccacttctgg acaaccacag acacaagaga taaccaagcc aaataacgga
    15181 gcaggccctt cagtaccctc agtgggtcag aatactccca ctgcagcctt aacatccgcc
    15241 aggggctcag gctcctccgt ctacaactca cgtcgcacat ccatcgacaa cagcgtggga
    15301 tccgacatgc acctgcacac tgccactaat cttgaaggga cagtgagcaa cccggatccg
    15361 atcccgactg aggcatcggt tggaattacc attgctacgg gtcatgagaa gcaactctcc
    15421 aagcagccca gtttggaaaa gccttctgcc acttccattt taacgaacaa cagtgatcct
    15481 ccgcaaagga atcccagcaa tggatcaatt aaccaaaatt caatagctga tttggaaaag
    15541 aaattggcag ctttgcgaaa taccgaaaac acagaagagt ctgcaacagc aacgttgtca
    15601 gtggttccta aacaagttga agaggtagtc aatccaagcg cgcgtaaaat ttcgcgtttt
    15661 agtgtcagtc gtgtgcagga gcagaaaact tcaaccggag ttgaggaacc agctcaaggt
    15721 caacttaaga tcgacctcca agtcgccggc cctggaggcc aggtgcagac caacaactcc
    15781 gttcagaacg gcagcgtggt caacactcca accgaagtca tcagctctcc tattcaaaat
    15841 gttccgctgg ccattaacgg aattcagttg atgtaccaac agccccagca gatacaccag
    15901 ttgccaggtg gaacatcaac tactacaagt ggagcaggga ctcagtgcat tgtagtgccg
    15961 caagtggtca atcaaaatgg cacccacgtg caacagccgt ctaacctaca gccacagcag
    16021 cagagcgtac atccaaatat gactcaacag ccacagcaga cgccacttaa tgggcaccca
    16081 tctatggtta acactcttca gcagcagcca ccacaacaat ccttaccgat gcagacgatt
    16141 cagtcgcagc agcagcagca taaccaaatg cctatcattt cgcaacaaca gcagcagcag
    16201 atcctaatgc agcagcaaca gcaacaggga tcccagcaag gatcacagca atttaatttg
    16261 cctggcacac aacagactca tcctcagcac cagttcatcc agtcgcagcc caaccaactt
    16321 cactcgcttc cgccccaggt agtgagcatg gctcagggaa atctgccgca tcagcttcca
    16381 cccatgcaga ctatgggagc ccaacagcaa atgatctcgc aacaacagca tcagttgcag
    16441 atgcagtcgc atatgcacca acaaaacgtg cccggaatgt acaaccagca gactggagct
    16501 cgtgtggcag caccccaaaa tttcgcaggt gcagttccaa atcatttgtt gcagcagtcc
    16561 cctttgatgg catcccagca gccagcacag cccatgcaac acgttatgca accgattttc
    16621 aatgcaacgt ccggtgaagt taccgaagag cctgtctctt tagcagctac gcatccgcat
    16681 ctgctaccga gcgatattca atccgatata aagcacaatt tagactcatt agtgaaccaa
    16741 ttgtgcaata cgcgcttggg tactaaccag caccaacggc tgttgctgtt gcgtcagaga
    16801 caactcatcg aagaggacga gctgcgtctt aaacattatg tggaatacga aaagttccaa
    16861 aaggcactgc gccaatctat tagcacaaac gttccagcta atgcagctta ctattctgcg
    16921 gctgccgcac agcttccaac taatttggca gccccagcag ctccgtcctc atcgaatccg
    16981 acgtccacta gctccgcaaa cacataac
//
