LOCUS       sequence-403136-       16952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fly transformation vector expressing indicated Wnk variant under 
            tubulin promoter for ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16952)
  AUTHORS   Jenny Lab
  TITLE     Jenny Lab Wnk Plasmids 2023
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 16952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pAttB-Tub-HA-WnkDeltaCC2
FEATURES             Location/Qualifiers
     source          1..16952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    156..237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(193..212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     247..266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    338..407
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(626..644)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(665..681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(665..681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(678..700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    689..705
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(713..719,720..737,738..743))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    758..779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(896..913)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1067..1655)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1147..1166)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1826..2617,2618..2686))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2449..2468
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2687..2791)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2817..3272)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2954..2975)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3166..3185
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3399..3421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3413..3430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3414..3430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3437..3456
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3437..3455
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3489..3507)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3959..4030,4432..4705,4780..5434,5496..5811,6015..6146,
                     6217..6831)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7606..7639
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             10307..10333
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctagagtcga cctcgaacgt taacgttaac gtaacgttaa ctcgagctag atcataatca
       61 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      241 ctagttgtgg tttgtccaaa ctcatcaatg tatgtcgagg tcgacctgca gcccaagctg
      301 ctagagatcc actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
      361 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
      421 tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct
      481 cgccctcgaa accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg
      541 cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga
      601 cactagttct agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
      661 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      721 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      781 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      841 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     2821 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2881 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2941 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3001 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3061 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3121 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3181 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3241 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     3301 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3361 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     3421 gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacgt accgggcccc
     3481 tagtatgtat gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt
     3541 ttttttttac tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat
     3601 ccaagggtat ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac
     3661 tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc
     3721 gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct
     3781 tcagttgcat tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta
     3841 aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga
     3901 ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat
     3961 gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca
     4021 tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact
     4081 gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc
     4141 gcaattgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata
     4201 taagacaatt tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta
     4261 tattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa
     4321 aaataaataa aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt
     4381 tagatgagca taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc
     4441 ggagcggctt cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg
     4501 ctccggccac ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac
     4561 ctcacctatg cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga
     4621 tggcggcagc tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg
     4681 cccaggaaac atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga
     4741 aatcttaata aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg
     4801 gcgaactttt ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc
     4861 ttgcctttcg atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg
     4921 gccaacctgt ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc
     4981 tctttatcgg ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc
     5041 cacgacatct gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt
     5101 cgctcagcaa atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg
     5161 gcggagaaag gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga
     5221 tctgcgatga gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc
     5281 tgaagaagct gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg
     5341 agctgtttga gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg
     5401 gcactcccag cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt
     5461 atctagtatt acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac
     5521 tacaatccgg cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag
     5581 tcccgtgatc ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat
     5641 atggagcagt tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg
     5701 tacacctaca aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg
     5761 tcggtgctca aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt
     5821 tccagtggaa acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta
     5881 gatagaattg cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata
     5941 gttttgcaga agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac
     6001 attttaattt gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca
     6061 cgcaagtggg tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct
     6121 ttcaaaacgt ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat
     6181 aatttactaa ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt
     6241 tttatgaggg aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg
     6301 attgccgaat tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg
     6361 atgatcggac tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg
     6421 gtggccaatg tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg
     6481 atggcgctgt ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc
     6541 ttgaactcgg gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt
     6601 tacgccaacg agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc
     6661 tgcacatcgt cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac
     6721 ttctccgccg ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc
     6781 cgggtgctcg catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat
     6841 atccgaaata actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat
     6901 tgccccctca aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat
     6961 agaatacaag tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa
     7021 ccaaaagact tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac
     7081 accgggatat attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc
     7141 tccaccacac cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac
     7201 attcaccttt tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg
     7261 ctcaaatggt tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat
     7321 gtacaattta tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta
     7381 atttaatatt atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta
     7441 caatttgaac tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa
     7501 tggtgggcat aatagtgttg tttatatata tcaaaaataa caactataat aataagaata
     7561 catttaattt agaaaatgct tggatttcac tggaactagg ctagcataac ttcgtataat
     7621 gtatgctata cgaagttatg ctagcggatc cgggaattgg gaattcgata tcaagcttgc
     7681 acaggtcctg ttcgataacg tcgtactcgg gaatcacctt taacttgtcc gctattgggt
     7741 atttgtcctt aaacacattc gtaatctctc ccacaatcat tgccctggcc tctgcaatgt
     7801 aaaaagtatt tataaaatat cgctggctct aaaggaaaca tattaatcaa agtattgagg
     7861 ctttatcaat ctaacattta caagtagtca gaccacaatt tttagactgc acttctgcgt
     7921 aagtaaatga aatgaaatgg tgacctactc ttgggtagcc gtactgttat atgctgacga
     7981 gccttcacct gggcctgctt tttctcctgt ttcgtctgat gcgtcggatg cggctggact
     8041 ggctgattgt tgggattggg ttttggatgc tgctccgaaa ggtgggccac agcttttgcg
     8101 atggtggtgc ccactatgcg gttgctgcag gaactacagc tgaatgttcg ctttattgtc
     8161 tgcagctcct ggggctggac gaaggccacg ccctcacccg gtgcggccat ctcctgcggc
     8221 aaaacgtatg tgggattcct gtccaagtag ctctcgatgc ccaccataat gcgctccagc
     8281 aggttggcca ggtgcgggtc tagctgcggc ggattcgtcg tctggttcac tgagtttagg
     8341 gtctgcagaa agaagtgtgc ctccatggta agcggattca tctgcacgat cagcgggctt
     8401 tagtctaaaa attcgaatga tacaaactcg tactcacgta aagcagtttg tttggcatca
     8461 tctcggccat cgtcttattc ttcttcgtgg cctttgttac cggagcggct atgttgggcg
     8521 cggtggagcc cggaagctga aaggttaatc gaatgcgtta acttttctgc aactcgaaag
     8581 tttgccgcct ttgttcgact gccaataact gttgattcga aaattcgaat cgaagcggtt
     8641 gaatttcgta gggtggccaa ctacaccaaa gttcgccggc ggtgtatgca tgtgagtgtg
     8701 agtgtgacag tgggtgtacg tgtgctttaa gcgacacaat cgcaccgcga tgtacatatt
     8761 tttgtagtaa tgtgtgtgca tttttcgtcc ggatcacggc tccataaaat aaatcatatc
     8821 acagggctca gggatttgtg agtagaagtt cccgccactt aaacgtcact tgtgaaatcg
     8881 ccatcgacgg tcaactttta ctcacactgg tctttcgcgg acggaccgtc tcaaagtact
     8941 gcctttctgc gttggaaaac atcgcctttt tcgtccaaaa ggagtcccca ggttcgatcc
     9001 gcatggcgtt gtgcgtgcgt gcctttcttt tcaaatgatt acggctatta acttgggggc
     9061 gttaagttgg aaacacgtaa attgcagact gcgattagag tgaccatgag taggagttca
     9121 aaatctcctg acatcatttt cttaaaacct gctttgtttt ttacatttct atttaatata
     9181 actcctattt gaataaaaaa acaaaacaag tttagatgtt aagatattaa ctacatcctt
     9241 tgctccaaag ggagagggga agttatggag ttaattaatt tgctgttgga aatcaatatg
     9301 gagtcagaaa tataatgatt tactaaacct tattgaatcg gtaacgatgc gaatttatat
     9361 taaaatagct tttatgaaac attcaacaaa aatattatta atgttggccc actttagcaa
     9421 ccggttaggt ctaccggttg ggcaagcaaa gattcacgcc ctggttcgag tcccaactag
     9481 tcctgcaaaa taccgcagca agttttagag agaccaagtg ccattacctc tcccacttca
     9541 gttatcggtt atgcggcgtt taagtcgaca gcttgccgtc tctagctccg gtgcctatat
     9601 aaagcagccc gctttccaca tttcatattc gttttacgtt tgtcaagcct catagccggc
     9661 agttcgaacg tatacgctct ctgagtcaga cctcgaaatc gtagctctac acaattctgt
     9721 gaattttcct tgtcgcgtgt gaaacacttc caataaaaac tcctgcaggt gagtacttta
     9781 aaaaaaaatc tagtgaaata atgctgaaaa gaaatttgtg tgggcaaaat tcaatgggca
     9841 aaaacgcgat gcggcttttt ctcaaaatgg cggccggcct gcgttttttc ctcaaaagtg
     9901 atgacgtcat gcctgttttt ttttttttgt tcgcaatgag gaatggctct taaaatctac
     9961 tagataaaaa aaatattcat tatttctatg ctgctggaac gcttcattaa tcttaaaaat
    10021 tctaaattcg gttaccatga tacttcgacg cataactgta gattttggat agaattaaag
    10081 agaaaatggc gagagagtaa aattccggcg tcggcaaagt agagcaaaaa aatcagtata
    10141 ccatttagct acctctctca ctcgcacgca gtgccggctc aagttgggcg cggctctgca
    10201 attatcgatt ttcttggggt gtgtaactaa tcatccgttt tcccttcctc ctcatccaca
    10261 gcgtgaaggt acccgcccgg ggatcagatc cgcggccgcc accatgtacc cctacgacgt
    10321 gcccgactac gcgatggata gcaaaataga acctgcagat gccataagca accagaaagc
    10381 tggtcttact tcgggcaaac ctaaaaaaga caagtcagtc aaggggacgt cgcaagcggt
    10441 tgtttcagac accaagaaaa tggaggccag aaagtcctcc tcggacacgg tgatcgagcc
    10501 attgatcaag cagcaggcgc cactgcactc gtcaaagacc acgccaacga ctttgacggc
    10561 gaacttcgtg caaaacattc gattcgtccg taaaaatgtg gaacagtcag gcaggaacac
    10621 caatccactg cagttcgttg aactcgagac agagtttcct cgtgactatg acgacaacat
    10681 cgagatgctt tcccgcgagg cggagcatct ggaggagcag ttccgcacgc ctacgcgctc
    10741 caatgctacg gatgccactt cccaccatgt ggccggaatc atcgacaaca ttataaccga
    10801 agcgtcacgg agtcttacaa tcgaaaagac tgaagacgat gtgccattga agtcttctac
    10861 caagcactca agcggcgtaa aacgagttgg ctttcaagta gaggataagg acgataccga
    10921 agttcagagc gaaaagcagg caaagagctt cgacgacata accaaaaaag cggagagctc
    10981 cgaggctagc gcagaggaag ctgctgtcac cgggtcctcg acagatgcat ctgcatctcc
    11041 cctgccctcc acgtcgctgg tctccaccac atcttcagct acatcgataa ccaagagtaa
    11101 gagcgatgag gacgatgatc cggtggccat gtcgccgtgc ggacgattct ttaagtacga
    11161 taaggaagtc ggacgcggtt cctttaaaac tgtctatcgt ggcctggaca cgttgactgg
    11221 tgttccagtt gcctggtgcg agttattgga caagcaagta aaaaaaagtg aacgtactag
    11281 atttcgcgag gaggcagaca tgctgaagaa acttcagcac ccaaacatag tgcggtttta
    11341 tacatattgg gagtttccta ttggccgcaa gaagaatatt gtactagtca ccgaactaat
    11401 gttatctgga actttgaaat cctatttgaa acgattcaaa aaaatacacc caaaggtatt
    11461 gaagtcatgg tgtcgccaga tactaaaagg ccttaacttc ctgcatactc gtcaatttcc
    11521 tataattcat cgtgatttga aatgtgataa tatttttata actggcacca ctggtagtgt
    11581 taaaatcggc gacttgggcc tggcaacttt gaaaaaccgc tcccacgcga aatctgtgat
    11641 tggtacgccg gagtttatgg ccccggaaat gtacgaggag cactacgacg agtcggtgga
    11701 cgtatatgca ttcggcatgt gcatgttgga gatggccatt tccgaatatc cttacagtga
    11761 gtgcaagggt cctgcacaga tctacaaaaa ggtgatatcg ggcattaagc cagcggcgtt
    11821 ggctaaggtg gaggatccca acgtgcgcga cattatcgaa cggtgcattg aacttaaaaa
    11881 ggaggatcgc cccagctgca atgaactgct agaatcggag ttctttgacg aagatatcgg
    11941 tatacgagtg gagccaactg cttcggagca gttcctttct gatccgagca tcagcatcat
    12001 cgagttccga ctgcggttta tggatcccaa gaagcgctcg tcccgtcata aggaaaacga
    12061 ggcaattcag tttgagtaca acatcaggca tgacgagtac gaacagattg cgcaagaaat
    12121 gatgaaggaa aatattatat ctgaggacga ctctcgcgct gtagctcggc ttttaaaagt
    12181 gcaggtggtg tcgttgctta aggaacgtgc ccagcgtcaa actcaaatca agctgcaaaa
    12241 cgagaagtct cgtttggaaa aattggcatt gcaaaagcaa cgtgagagtt tgccgactaa
    12301 cgtcgatgaa gacgaggaag aggaggagga gtccgaggac gaggaggatg gtgttaaatg
    12361 gaatcagcgc ctgcagctaa gatatgacct gcttaacact gattcagaaa caagcctggc
    12421 tctatccacg aacagcgtcg aaccgcagca gctgtccact cgctcgaaca cttcgattcc
    12481 gaacagtggc attcagcaac ctgttcaggt cccaggtcaa gtgcctgttt cccagttgat
    12541 ctcggtgcaa ccacaggcta ttccgtcacc ggctattccc atgcaacaga agccgacggt
    12601 gcactacatt caaccgccac agctggcctc ttatcaaaat tcgaatgcat cgatgcagga
    12661 gatgaccaat aaccaggtca tttcgccgac aggcagtcaa caaatgcaac agcaacaacc
    12721 cgtagtggca ccaacagtca atcatcaggt catgccgcag cagcaagtga accaacaaca
    12781 gcaacaacca caaatgatgc agcaaatacc ccagcaagtt caagtgcaac agccccaaac
    12841 tgtattgcct ccgcagccac atgagcagca gccacagcaa caacaacagc cactgcagca
    12901 gcaactgcag cagcttatgc atactaacgt gcaggctccg gatttgactc aacagcaaca
    12961 aatggctcag caacaggccc agcaatattt tcagcagcag cagcaacagc ctcagcaggc
    13021 cgttaacatg caacaagctt atgcaatgca gcaagctggc caacagcaac agctttcgca
    13081 gcctcttcaa attcagcaac agatcttgca gcaacaacaa gtggccgttt cacatcagca
    13141 acagataatg cagcaacaac tgatgcctca gcaacaacat cagcaactcg ttacgggatc
    13201 gcaagtcatg gcaccgcatc aacatcagca acctattcag atcccagtgc agatgcaagt
    13261 tccaccgacg tccgtggctc ctccaataca acacacttac aatcagcagg gcggacaagt
    13321 aacgctcagt gatgcccaac agcaacagca tcctggattc tcggccgtac cacaacaggc
    13381 agcgccattt atacaacagc ccacacagca acccattcag ctatcaatgc ctttggaaca
    13441 acaattgcag caattgctgc atagccagcc agcccagcaa caacaggcca tgagccaaca
    13501 gcagcaacag tctatggtcc aacagcagca acagcccttg gtgcaacagc aacaactgcc
    13561 tttggtacaa cagcagccgc ctttggtcca gcagcagcaa ccgctggttc agcatcagca
    13621 gccttcggtc caacatcagc aacctttggt gcaacagccc cagcaacagc aaccacagcc
    13681 ccagaatcag caaccacagc cccagacaca gcaaacacac gtggttcagc aacaaccacc
    13741 acagcagcaa cctgcagtag agcaaatctc acagatttct tcgcaagtgc ctgttcaaca
    13801 ggaaaatctg cagccaatac aggtcaacaa agatgcgaat gtcgcaacgg atgcaatgtc
    13861 cctaaatagc gcccacggtg ccctcgaacc tgcaccgaag accgaacccc agaactctgc
    13921 tgatgcggaa aagcagcaaa agcagacagg cacgggcaca cgttcccaga aaccgaggcg
    13981 atcaaaccgg agcggcaacg aacggattcc caaacttagt gtaaccagcg tcgacgaggg
    14041 cagcgtcata aactgtcaca tggaaaataa acttaaaacg attacattta aattcgacat
    14101 aggagacgta aatccggttg aaatagccaa taaattgatc gcccaagatt tgctttctaa
    14161 ttgtcaaagt acagtttttg tggaaatgat caatgaaatt gttgaccaag tcaaacagaa
    14221 tcccaatcaa atccctatac cgacaaatta tcgccgcaat attgaaaagc gggatgaaac
    14281 cgccagcgac atcacaaaga tgttcgagcc cactatccat ggtgttgaaa ccctgccatc
    14341 cggtggagga ggcgctgaac agtcaaactg caacttgact ttagaggcca ggtcccattt
    14401 atccaacata ccaaatgcga aagaaccaca gctgaacgtc agcactccgc ccacaaccac
    14461 ttcgactatg tcttcctcat caaccgcatc gagggatgcg cccaatagta gcaatgacgt
    14521 gaccatcggc tctggttccg tttctcgaaa aacgagcacg gcctcggagt acacttcgct
    14581 atcgattgac tatatgcccg acagcaacat tactccaact ggtccggagc caccgcttga
    14641 cgatggcaag gataaaaaac cttgctccct tgctatagcc agaatgcaaa agctcttgga
    14701 gtcggccggc aatggtgctc ctcgcagcct gaatttgccc ttgaatcgtc acctgaaaat
    14761 tcaagaggac ttgaagcata ctagaagtct tgacgatttg accgcagtga aaataacctt
    14821 tgacatgccc aacaaggctg cattggacac ttcggaaaac gctcagcagg ccacagaagc
    14881 ggaagcggag aaaccaaaag ataaatccgg acaggcagta ggcaaccagg gaactggagc
    14941 ggccaacact ctagagcagt taaaaataga actggagaac attactcatg cccacgcgtt
    15001 tgcctccgct gtagtggcct cgattaacaa cagagctcct catcaagctt ccccagcaat
    15061 gtcctcccta aaggccactt ctggacaacc acagacacaa gagataacca agccaaataa
    15121 cggagcaggc ccttcagtac cctcagtggg tcagaatact cccactgcag ccttaacatc
    15181 cgccaggggc tcaggctcct ccgtctacaa ctcacgtcgc acatccatcg acaacagcgt
    15241 gggatccgac atgcacctgc acactgccac taatcttgaa gggacagtga gcaacccgga
    15301 tccgatcccg actgaggcat cggttggaat taccattgct acgggtcatg agaagcaact
    15361 ctccaagcag cccagtttgg aaaagccttc tgccacttcc attttaacga acaacagtga
    15421 tcctccgcaa aggaatccca gcaatggatc aattaaccaa aattcaatag ctgatttgga
    15481 aaagaaattg gcagctttgc gaaataccga aaacacagaa gagtctgcaa cagcaacgtt
    15541 gtcagtggtt cctaaacaag ttgaagaggt agtcaatcca agcgcgcgta aaatttcgcg
    15601 ttttagtgtc agtcgtgtgc aggagcagaa aacttcaacc ggagttgagg aaccagctca
    15661 aggtcaactt aagatcgacc tccaagtcgc cggccctgga ggccaggtgc agaccaacaa
    15721 ctccgttcag aacggcagcg tggtcaacac tccaaccgaa gtcatcagct ctcctattca
    15781 aaatgttccg ctggccatta acggaattca gttgatgtac caacagcccc agcagataca
    15841 ccagttgcca ggtggaacat caactactac aagtggagca gggactcagt gcattgtagt
    15901 gccgcaagtg gtcaatcaaa atggcaccca cgtgcaacag ccgtctaacc tacagccaca
    15961 gcagcagagc gtacatccaa atatgactca acagccacag cagacgccac ttaatgggca
    16021 cccatctatg gttaacactc ttcagcagca gccaccacaa caatccttac cgatgcagac
    16081 gattcagtcg cagcagcagc agcataacca aatgcctatc atttcgcaac aacagcagca
    16141 gcagatccta atgcagcagc aacagcaaca gggatcccag caaggatcac agcaatttaa
    16201 tttgcctggc acacaacaga ctcatcctca gcaccagttc atccagtcgc agcccaacca
    16261 acttcactcg cttccgcccc aggtagtgag catggctcag ggaaatctgc cgcatcagct
    16321 tccacccatg cagactatgg gagcccaaca gcaaatgatc tcgcaacaac agcatcagtt
    16381 gcagatgcag tcgcatatgc accaacaaaa cgtgcccgga atgtacaacc agcagactgg
    16441 agctcgtgtg gcagcacccc aaaatttcgc aggtgcagtt ccaaatcatt tgttgcagca
    16501 gtcccctttg atggcatccc agcagccagc acagcccatg caacacgtta tgcaaccgat
    16561 tttcaatgca acgtccggtg aagttaccga agagcctgtc tctttagcag ctacgcatcc
    16621 gcatctgcta ccgagcgata ttcaatccga tataaagcac aatttagact cattagtgaa
    16681 ccaattgtgc aatacgcgct tgggtactaa ccagcaccaa cggctgttgc tgttgcgtca
    16741 gagacaactc atcgaagagg acgagctgcg tcttaaacat tatgtggaat acgaaaagtt
    16801 ccaaaaggca ctgcgccaat ctattagcac aaacgttcca gctaatgcag cttactattc
    16861 tgcggctgcc gcacagcttc caactaattt ggcagcccca gcagctccgt cctcatcgaa
    16921 tccgacgtcc actagctccg caaacacata ac
//
