LOCUS       sequence-403140-       15989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Measures mPing transposition in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Renken K, Mendoza SM, Diaz S, Slotkin RK, Hancock CN
  TITLE     Pol V produced RNA facilitates transposable element excision site 
            repair in Arabidopsis.
  JOURNAL   MicroPubl Biol. 2023 May 16;2023:10.17912/micropub.biology.000793. 
            doi: 10.17912/micropub.biology.000793. eCollection 2023.
  PUBMED    37273575
REFERENCE   2  (bases 1 to 15989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-05-16"; volume: 
            "2023:10.17912/micropub.biology.000793. doi"; pages: " 
            10.17912/micropub.biology.000793. eCollection 2023"
COMMENT     Sequence Label: pEarleyGate 100RMOA mPing
FEATURES             Location/Qualifiers
     source          1..15989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        493..838
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     781..801
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1381..2091
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1408..1436)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2031..2051
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2123..2375
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4238..4262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5711..5764
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(7146..7170)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7223..7930
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(7955..7972)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7955..7971)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7964..7986)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8174..8198
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9499..10128
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10557..11630
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11696..11890
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12126..12148)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12234..12374
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12289..12308
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12389..12406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12560..13148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12640..12659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13235..14029)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14454..14478
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(14564..14816)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(14826..15377)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(15383..15763)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cgcgtagatt agccttttca atttcagaaa gaatgctaac ccacagatgg ttagagaggc
       61 ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg aaatcaaata
      121 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac
      181 agagaaagat atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
      241 gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcccactga
      301 atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg ccgtaaagac
      361 tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa
      421 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
      481 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
      541 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
      601 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
      661 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
      721 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
      781 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
      841 ggggactcta gataaggcca gtcacaatgg gggtttcact ggtgtgtcat gcacatttaa
      901 taggggtaag actgaataaa aaatgattat ttgcatgaaa tggggatgag agagaaggaa
      961 agagtttcat cctggtgaaa ctcgtcagcg tcgtttccaa gtcctcggta acagagtgaa
     1021 acccccgttg aggccgattc gtttcattca ccggatctct tgcgtccgcc tccgccgtgc
     1081 gacctccgca ttctcccgcg ccgcgccgga ttttgggtac aaatgatccc agcaacttgt
     1141 atcaattaaa tgctttgctt agtcttggaa acgtcaaagt gaaacccctc cactgtgggg
     1201 attgtttcat aaaagatttc atttgagaga agatggtata atattttggg tagccgtgca
     1261 atgacactag ccattgtgac tggcctaatc tagaggatcc aaggagatat aacaatgaag
     1321 actaatcttt ttctctttct catcttttca cttctcctat cattatcctc ggccgaattc
     1381 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1441 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1501 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1561 actactttct cttatggtgt tcaatgcttt tcaagatacc cagatcatat gaagcggcac
     1621 gacttcttca agagcgccat gcctgaggga tacgtgcagg agaggaccat cttcttcaag
     1681 gacgacggga actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac
     1741 aggatcgagc ttaagggaat cgatttcaag gaggacggaa acatcctcgg ccacaagttg
     1801 gaatacaact acaactccca caacgtatac atcatggccg acaagcaaaa gaacggcatc
     1861 aaagccaact tcaagacccg ccacaacatc gaagacggcg gcgtgcaact cgctgatcat
     1921 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1981 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     2041 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa acatgatgag
     2101 ctttaagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     2161 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     2221 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     2281 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     2341 attatcgcgc gcggtgtcat ctatgttact agatcgggag atctcctttg ccccggagat
     2401 tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga
     2461 cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc
     2521 tgacccacag atggttagag aggctagttc gtgagtagat atattactca acttttgatt
     2581 cgctatttgc agtgcacctg tggcgttcat cacatctttt gtggcactgt ttgcactggt
     2641 cattgctatt acaaaggacc ttcctgatgt tgaaggagat cgaaagtaag taactgcacg
     2701 cataaccatt ttctttccgc tctttggctc aatccatttg acagtcaaag acaatgttta
     2761 accagctccg tttgatatat tgtctttatg tgtttgttca agcatgttta gttaatcatg
     2821 cctttgattg atcttgaata ggttccaaat atcaaccctg gcaacaaaac ttggagtgag
     2881 aaacattgca ttcctcggtt ctggacttct gctagtaaat tatgcttcag ccatatcact
     2941 agctttctac atgcctcagg tgaattcatc tatttccgtc ttaactattt cggttaatta
     3001 aagcacgaac accattactg catgtagaag cttgataaac tatcgccacc aatttatttt
     3061 tgttgcgata ttgttacttt cctcagtatg cagctttgaa aagaccaacc ctcttatcct
     3121 ttaacaatga acaggttttt agaggtagct tgatgattcc tgcacatgtg atcttggctt
     3181 caggcttaat tttccaggta aagcattatg agatactctt atatctctta catacttttg
     3241 agataatgca caagaacttc ataactatat gctttagttt ctgcatttga cactgccaaa
     3301 ttcattaatc tctaatatct ttgttgttga tctttggtag acatgggtac tagaaaaagc
     3361 aaactacacc aaggtaaaat acttttgtac aaacataaac tcgttatcac ggaacatcaa
     3421 tggagtgtat atctaacgga gtgtagaaac atttgattat tgcaggaagc tatctcagga
     3481 tattatcggt ttatatggaa tctcttctac gcagagtatc tgttattccc cttcctctag
     3541 ctttcaattt catggtgagg atatgcagtt ttctttgtat atcattcttc ttcttctttg
     3601 tagcttggag tcaaaatcgg ttccttcatg tacatacatc aaggatatgt ccttctgaat
     3661 ttttatatct tgcaataaaa atgcttgtac caattgaaac accagctttt tgagttctat
     3721 gatcactgac ttggttctaa ccaaaaaaaa aaaaatgttt aatttacata tctaaaagta
     3781 ggcttaggga aacctaaaca gtaaaatatt tgtatattat tcgaatttca ctcatcataa
     3841 aaacttaaat tgcaccataa aattttgttt tactattaat gatgtaattt gtgtaactta
     3901 agataaaaat aatattccgt aagttaaccg gctaaaacca cgtataaacc agggaacctg
     3961 ttaaaccggt tctttactgg ataaagaaat gaaagcccat gtagacagct ccattagagc
     4021 ccaaacccta aatttctcat ctatataaaa ggagtgacat tagggttttt gttcgtcctc
     4081 ttaaagcttc tcgttttctc tgccgtctct ctcattcgcg cgacgcaaac gatcttcagg
     4141 tgatcttctt tctccaaatc ctctctcata actctgattt cgtacttgtg tatttgagct
     4201 cacgctctgt ttctctcacc acagccggat cgagatcaca agtttgtaca aaaaagcagg
     4261 cttcatgcag agtttagcca tctctctact cctctcagaa actcattccc tcttttctca
     4321 tacgaagacc tcctcccttt tatctttact gtttctctct tcttcaaaga tgtctgagca
     4381 aaatactgat ggaagtcaag ttccagtgaa cttgttggat gagttcctgg ctgaggatga
     4441 gatcatagat gatcttctca ctgaagccac ggtggtagta cagtccacta tagaaggtct
     4501 tcaaaacgag gcttctgacc atcgacatca tccgaggaag cacatcaaga ggccacgaga
     4561 ggaagcacat cagcaactag tgaatgatta cttttcagaa aatcctcttt acccttccaa
     4621 aatttttcgt cgaagatttc gtatgtctag gccacttttt cttcgcatcg ttgaggcatt
     4681 aggccagtgg tcagtgtatt tcacacaaag ggtggatgct gttaatcgga aaggactcag
     4741 tccactgcaa aagtgtactg cagctattcg ccagttggct actggtagtg gcgcagatga
     4801 actagatgaa tatctgaaga taggagagac tacagcaatg gaggcaatga agaattttgt
     4861 caaaggtctt caagatgtgt ttggtgagag gtatcttagg cgccccacca tggaagatac
     4921 cgaacggctt ctccaacttg gtgagaaacg tggttttcct ggaatgttcg gcagcattga
     4981 ctgcatgcac tggcattggg aaagatgccc agtagcatgg aagggtcagt tcactcgtgg
     5041 agatcagaaa gtgccaaccc tgattcttga ggctgtggca tcgcatgatc tttggatttg
     5101 gcatgcattt tttggagcag cgggttccaa caatgatatc aatgtattga accaatctac
     5161 tgtatttatc aaggagctca aaggacaagc tcctagagtc cagtacatgg taaatgggaa
     5221 tcaatacaat actgggtatt ttcttgctga tggaatctac cctgaatggg cagtgtttgt
     5281 taagtcaata cgactcccaa acactgaaaa ggagaaattg tatgcagata tgcaagaagg
     5341 ggcaagaaaa gatatcgaga gagcctttgg tgtattgcag cgaagatttt gcatcttaaa
     5401 acgaccagct cgtctatatg atcgaggtgt actgcgagat gttgttctag cttgcatcat
     5461 acttcacaat atgatagttg aagatgagaa ggaaaccaga attattgaag aagatgcaga
     5521 tgcaaatgtg cctcctagtt catcaaccgt tcaggaacct gagttctctc ctgaacagaa
     5581 cacaccattt gatagagttt tagaaaaaga tatttctatc cgagatcgag cggctcataa
     5641 ccgacttaag aaagatttgg tggaacacat ttggaataag tttggtggtg ctgcacatag
     5701 aactggaaat gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     5761 ccctatggat ccgtcgccgg ccgtggatcc gtcgccggct gctgaaaccc ggcggcgtgc
     5821 aaccgggaaa ggaggcaaac agcgcggggg caagcaacta ggattgaaga ggccgccgcc
     5881 gatttctgtc ccggccaccc cgcctcctgc tgcgacgtct tcatcccctg ctgcgccgac
     5941 ggccatccca ccacgaccac cgcaatcttc gccgattttc gtccccgatt cgccgaatcc
     6001 gtcaccggct gcgccgacct cctctcttgc ttcggggaca tcgacggcaa ggccaccgca
     6061 accacaagga ggaggatggg gaccaacatc gaccatttcc ccaaactttg catctttctt
     6121 tggaaaccaa caagacccaa attcatgttt ggtcaggggt tatcctccag gagggtttgt
     6181 caattttatt caacaaaatt gtccgccgca gccacaacag caaggtgaaa attttcattt
     6241 cgttggtcac aatatggggt tcaacccaat atctccacag ccaccaagtg cctacggaac
     6301 accaacaccc caagctacga accaaggcac ttcaacaaac attatgattg atgaagagga
     6361 caacaatgat gacagtaggg cagcaaagaa aagatggact catgaagagg aagagagact
     6421 ggccagtgct tggttgaatg cttctaaaga ctcaattcat gggaatgata agaaaggtga
     6481 tacattttgg aaggaagtca ctgatgaatt taacaagaaa gggaatggaa aacgtaggag
     6541 ggaaattaac caactgaagg ttcactggtc aaggttgaag tcagcgatct ctgagttcaa
     6601 tgactattgg agtacggtta ctcaaatgca tacaagcgga tactcagacg acatgcttga
     6661 gaaagaggca cagaggctgt atgcaaacag gtttggaaaa ccttttgcgt tggtccattg
     6721 gtggaagata ctcaaaagag agcccaaatg gtgtgctcag tttgaaaaga ggaaaaggaa
     6781 gagcgaaatg gatgctgttc cagaacagca gaaacgtcct attggtagag aagcagcaaa
     6841 gtctgagcgc aaaagaaagc gcaagaaaga aaatgttatg gaaggcattg tcctcctagg
     6901 ggacaatgtc cagaaaatta tcaaagtgac gcaagatcgg aagctggagc gtgagaaggt
     6961 cactgaagca cagattcaca tttcaaacgt aaatttgaag gcagcagaac agcaaaaaga
     7021 agcaaagatg tttgaggtat acaattccct gctcactcaa gatacaagta acatgtctga
     7081 agaacagaag gctcgccgag acaaggcatt acaaaagctg gaggaaaagt tatttgctga
     7141 ctaagaccca gctttcttgt acaaagtggt gcctaggtga gtctagagag ttaattaaga
     7201 cccgggacta gtccctagag tcctgcttta atgagatatg cgagacgcct atgatcgcat
     7261 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga
     7321 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt
     7381 atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt
     7441 aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca
     7501 caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc
     7561 ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg
     7621 ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt
     7681 ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg
     7741 aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc
     7801 ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac
     7861 cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga
     7921 ggctcagcag gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
     7981 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8041 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8101 atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     8161 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     8221 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     8281 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     8341 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     8401 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     8461 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     8521 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     8581 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     8641 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     8701 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     8761 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     8821 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     8881 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     8941 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     9001 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     9061 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     9121 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     9181 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     9241 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     9301 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     9361 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     9421 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     9481 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     9541 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     9601 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     9661 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     9721 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     9781 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     9841 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     9901 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     9961 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
    10021 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
    10081 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
    10141 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
    10201 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
    10261 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
    10321 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
    10381 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
    10441 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
    10501 tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
    10561 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
    10621 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
    10681 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
    10741 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
    10801 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
    10861 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
    10921 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
    10981 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
    11041 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
    11101 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
    11161 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
    11221 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
    11281 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
    11341 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
    11401 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
    11461 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
    11521 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
    11581 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
    11641 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
    11701 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
    11761 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    11821 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    11881 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    11941 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
    12001 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
    12061 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
    12121 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    12181 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
    12241 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
    12301 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    12361 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    12421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    12541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    12601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    12661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    12721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    12781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    12841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    12901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    13021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    13081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    13141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    13201 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
    13261 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    13321 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    13381 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    13441 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    13501 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    13561 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    13621 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    13681 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    13741 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    13801 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    13861 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    13921 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    13981 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    14041 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    14101 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    14161 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    14221 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    14281 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    14341 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    14401 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    14461 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
    14521 ttaatgtact gaattaacgc cgaattaatt cgagctcgga tctgataatt tatttgaaaa
    14581 ttcataagaa aagcaaacgt tacatgaatt gatgaaacaa tacaaagaca gataaagcca
    14641 cgcacattta ggatattggc cgagattact gaatattgag taagatcacg gaatttctga
    14701 caggagcatg tcttcaattc agcccaaatg gcagttgaaa tactcaaacc gccccatatg
    14761 caggagcgga tcattcattg tttgtttggt tgcctttgcc aacatgggag tccaagattc
    14821 tgcagtcaaa tctcggtgac gggcaggacc ggacggggcg gtaccggcag gctgaagtcc
    14881 agctgccaga aacccacgtc atgccagttc ccgtgcttga agccggccgc ccgcagcatg
    14941 ccgcgggggg catatccgag cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg
    15001 atgacagcga ccacgctctt gaagccctgt gcctccaggg acttcagcag gtgggtgtag
    15061 agcgtggagc ccagtcccgt ccgctggtgg cggggggaga cgtacacggt cgactcggcc
    15121 gtccagtcgt aggcgttgcg tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg
    15181 ccgtccacct cggcgacgag ccagggatag cgctcccgca gacggacgag gtcgtccgtc
    15241 cactcctgcg gttcctgcgg ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg
    15301 ttgacgatgg tgcagaccgc cggcatgtcc gcctcggtgg cacggcggat gtcggccggg
    15361 cgtcgttctg ggctcatcga ttcgatttgg tgtatcgaga ttggttatga aattcagatg
    15421 ctagtgtaat gtattggtaa tttgggaaga tataatagga agcaaggcta tttatccatt
    15481 tctgaaaagg cgaaatggcg tcaccgcgag cgtcacgcgc attccgttct tgctgtaaag
    15541 cgttgtttgg tacacttttg actagcgagg cttggcgtgt cagcgtatct attcaaaagt
    15601 cgttaatggc tgcggatcaa gaaaaagttg gaatagaaac agaatacccg cgaaattcag
    15661 gcccggttgc catgtcctac acgccgaaat aaacgaccaa attagtagaa aaataaaaac
    15721 tgactcggat acttacgtca cgtcttgcgc actgatttga aaaatctcag aattccaatc
    15781 ccacaaaaat ctgagcttaa cagcacagtt gctcctctca gagcagaatc gggtattcaa
    15841 caccctcata tcaactacta cgttgtgtat aacggtccac atgccggtat atacgatgac
    15901 tggggttgta caaaggcggc aacaaacggc gttcccggag ttgcacacaa gaaatttgcc
    15961 actattacag aggcaagagc agcagctga
//
