LOCUS       sequence-403146-        6643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for bacterial expression of mouse protein ELMO3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6643)
  TITLE     Kahn Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGEX4-ELMO3
FEATURES             Location/Qualifiers
     source          1..6643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(182..187,188..203,204..210)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    218..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             257..910
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     868..890
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             917..934
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2692..2714)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2859..2877)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2945..3049
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3050..3118,3119..3910)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3268..3287)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4081..4669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4570..4589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4823..4840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        4913..4990
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4991..6073
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5010..5029)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(6122..6127,6128..6145,6146..6152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6160..6176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6184..6200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6184..6200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6196..6643,1..80)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(6216..6233)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6216..6232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6225..6247)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcttatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
       61 tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt
      121 ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct
      181 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac
      241 acaggaaaca gtattcatgt cccctatact aggttattgg aaaattaagg gccttgtgca
      301 acccactcga cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg
      361 cgatgaaggt gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct
      421 tccttattat attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat
      481 agctgacaag cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct
      541 tgaaggagcg gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt
      601 tgaaactctc aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga
      661 tcgtttatgt cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt
      721 gtatgacgct cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa
      781 attagtttgt tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc
      841 cagcaagtat atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca
      901 tcctccaaaa tcggatctgg ttccgcgtgg atccactagt gccgccacaa tggcgccccc
      961 gcggaatgtg gtgaagattg ctgttcagat gtctgacgcc atcccacagc ttatccagct
     1021 agaccaggca aagcccctgg ccactgtact gaaggaggtg tgtgacgcgt ggagcctgac
     1081 tcatccggag cactatgccc tgcagtttgc tgatgggcac aggaaataca tcacagagaa
     1141 taaccgcttg gagatcaaga atggaagcat cctgtgtctc agcactgccc cagatctgaa
     1201 ggcccagcag ctactgggta gactgcagaa tacaagtcgt gaagggtgct gtgaagtcct
     1261 gagaaacctg gtcctgctgg cctcagacat gacctttgcc caggaggtca tcagtcgtga
     1321 tgggcttcag aaactaagca ccatcattga aaatggggat gacctagggg agatgctggc
     1381 ccttggtcta agggctttct tggagctcat ggaacacggt gtagtgtcct gggagacact
     1441 cagcatctcc tttgtcagga aggtgatatc ttatgtgaac atgaacctga tggatgcctc
     1501 tgtccagccc ctggctctga gactgctgga gagtgtgacc ttgagcagtc ctgccctggg
     1561 ccagctggtg aagagtgagg tgccactgga taggctgctg gtgcacctgc aagtaatgaa
     1621 ccaccagctg caaaccaagg ccatggctct gttgacggcc ctgctgcagg gagccagccc
     1681 cactgaacgc aaggaaatgc ttgaccacct atggaagaag aatcttcgcc agttcatcta
     1741 caagaacatc atccacagtg caacacccat gggtgacgag atggctcatc acttgtatgt
     1801 gctgcaggct cttaccctag ggctgctgga gccacggatg cggacaccac ttgatcccta
     1861 cagccaggaa cagcgggacc agctgcaggc tctgcgccag gcagcctttg aaccagaggg
     1921 ggagtccttg ggcacagggc tcagtgcaga ccgccgccgg tctctctgtg tccgagagtt
     1981 ccgcaagtta ggcttctcta acagcaaccc agcccaggac ctggagcgtg tgccccccgg
     2041 cctgctggcc ctggacaata tgctctactt ctccagacat gcaccaagtg catacagtcg
     2101 gtttgtgttg gagaacagta gccgagagga caagcatgaa tgcccctttg cccggagcag
     2161 catccagctg acagcgttgc tgtgtgagct gctccgtgtt ggggagcctt gctccgagac
     2221 agcccaggac ttctcgccca tgttcttcag ccaagaccac agtttccatg agctcttctg
     2281 tgtggctatc cagctactga ataagacctg gaaggagatg cgggcaacac aggaagattt
     2341 tgacaaggtg atgcaagtgg ttcgggagca gctggcccgt accctggctc tgaagcccac
     2401 ctccctggag ctctttcgaa ccaaagtgaa tgccctcacc tatggggaag tgttgaggct
     2461 gcggcagaca gagcggctgc accaggaggg cacgctggcc cctcctatac tggaattgcg
     2521 agagaaactg aagccagagc tcatgggctt aatccgccag cagcgtttgc tccgactctg
     2581 tgaaggaatg ctcttccgca agatcagcag ccggagatag taaggtaccg tgcggccgca
     2641 tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     2701 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     2761 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     2821 tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac gcctattttt
     2881 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2941 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3001 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3061 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3121 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3181 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3241 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3301 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3361 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3421 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3481 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3541 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     3601 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3661 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3721 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3781 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3841 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3901 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3961 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4021 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4081 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4141 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4201 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4261 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4321 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4381 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4441 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4501 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4561 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4621 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4681 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4741 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4801 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     4861 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt ccgacaccat
     4921 cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
     4981 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
     5041 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
     5101 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     5161 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
     5221 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
     5281 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
     5341 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
     5401 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
     5461 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
     5521 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
     5581 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
     5641 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
     5701 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
     5761 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
     5821 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
     5881 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     5941 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     6001 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6061 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcaccccagg
     6121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6181 acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt
     6241 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6301 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6361 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
     6421 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
     6481 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt
     6541 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
     6601 caggaaggcc agacgcgaat tatttttgat ggcgttggaa tta
//
