LOCUS       sequence-403204-       10652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector to introduce an osTir1 expressing cassette at the 
            mouse TIGRE acceptor locus using Puromycin selection. 
            Auxin-inducible degron system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur 
            N, Dekker J, Mirny LA, Bruneau BG
  TITLE     Targeted Degradation of CTCF Decouples Local Insulation of 
            Chromosome Domains from Genomic Compartmentalization.
  JOURNAL   Cell. 2017 May 18;169(5):930-944.e22. doi: 
            10.1016/j.cell.2017.05.004.
  PUBMED    28525758
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.05"; journalName: "Cell"; date: "2017-05-18- 
            18"; volume: "169"; issue: "5"; pages: "930-944.e22"
COMMENT     Sequence Label: pEN395 -
            pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-...
FEATURES             Location/Qualifiers
     source          1..10652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1351..1486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    1591..1612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1627..1632,1633..1650,1651..1657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1665..1681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1689..1705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2048..2067
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2066..2121
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2167..2186
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2277..2318)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(2319..4043)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(4102..4121)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          4129..5145
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(4183..4205)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        5423..5726
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    5790..5823
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        5887..6386
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5922..5945)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6319..6338
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        6398..6445
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6464..7063
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6464..6483)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6960..6980
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7095..7112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7101..7325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7380..7413
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8431..8447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8455..8471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8479..8485,8486..8503,8504..8509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8524..8545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8662..8679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8833..9421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8913..8932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9592..10383,10384..10452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10215..10234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10453..10557)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10625..10643
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctaaccta ggcctccaaa aaagcctcct cactacttct
     1381 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     1441 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     1501 gcgggactat ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga
     1561 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1621 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1681 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg
     1741 atctccataa gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc
     1801 tggctagtaa aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac
     1861 aaaatataaa aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag
     1921 cagggggctg tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca
     1981 gcatatgggc atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat
     2041 ttgccctccc atatgtcctt ccgagtgaga gacacaaaaa attccaacac actattgcaa
     2101 tgaaaataaa tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc
     2161 cataattttt ggcagaggga aaaagatctc agtggtattt gtgagccagg gcattggcca
     2221 caccagccac caccttctga taggcagcct gcacctgagg ccgcttaccc gggtcaggtg
     2281 ctgtccaggc ccagcagggg gttggggatg ggcttgccca gaattttcac gaaattggga
     2341 gcgtcatctc gtgcgccagc tgtggtccga tagacgtaca gtttctccac tttgggcaga
     2401 tcgccgtggt tttcctccat ttcattacta ccgtcgcgtt cgttgatgac ctcaacattc
     2461 agcattggca ttttactagc caggacttgg catcccttga gggtcacgtt gcagcttgac
     2521 atccaaagtg acctcatcgt ctcgtaccgg gcgaaattgc ccagcagtgc ggcgtcccca
     2581 aaaggggaat cgcgaatttc gagttttctc aggttcttgc acccgttcat cacgtgcatc
     2641 atccctttat ctgaatctcc ggcgaatgcg attgacagca tttccagctg cttggcgtac
     2701 ttcccaatat acatgaacac tttgtcagtc agcaggccgg atatgctcag ccgctggaga
     2761 cccttacatt cgcgtactat ggcaccgaac ccttcgtcca gtggctgtga cgttacgacg
     2821 tcgggcttac cgggctccaa gatgcacagt ctaaaccttg tgaagtttgg acagtttttg
     2881 gcaacggtaa caagggcagc attggtcatc tgatgacaga agtacagcaa actattgagt
     2941 ttggggcacc ccagggacac tgctaccaat ccctcctcgg tgactgcgct gtatccggcc
     3001 acatagaaat ctgaagggaa caccctcagc tcctgcagat ctttgcagga ggaggccaca
     3061 acttgcagtc ccttgtctga aatacaatcc agtacccaga gcctttggag cttcacgcag
     3121 cggctgatca tcttagtaag gtcactagcg tcgagggttg gagcgtaact cagattaagt
     3181 ccggtcaact gtgcacacag gggatatata aaagacaggc agacagggct agcgtcccaa
     3241 aacccgctca gtgacctaag cattttgcat ttttccagag cactagtgag cttaaagtag
     3301 ctctctgtct ggaaatcatc ggtcagattt ccggtcccca gatcctccag gttgggagtc
     3361 cggagcaaaa tcttggcgag cgtgtccaca gaaacggatc tgttcagccg cagagatctc
     3421 aggttggggg atcgggaaac gagtctctcc aaactcccgg cattcacttc gcccttgata
     3481 caagcgaaat tcaagctcac caggctagtg cagctatccg ggaaacagct cagccaccga
     3541 ggtcccctat cctctacctc attttcctgc aggtccagtt ctcgcagcag tttacaatgg
     3601 ctagctacag cagccagacc gtcggtgcta aatccttcac agctgatcag caccagggcg
     3661 cggaatcggg ggaagctgcg ggcaagcagt tccagtgact cgtctgacac caccatcctt
     3721 ttcatcctga gttcttccag accatgacac ccgcgcgcag cggcctcgat ccagggcccg
     3781 gcgtagccgc cccagtccgg aggcaccaaa ttgaagtcgg caaagtgagg cttgcccttc
     3841 actgtaaggg ccctgacgtt cgggaaccgg gcggccactc gcccggctcg tacggcataa
     3901 caattaccca caaaaacacc tctgcggctc aagcgttcga tctcatacca gaccttgcac
     3961 accagggaca cggtattcct atctcgttgg gcgggaagga atgagaaaat atgttcgacc
     4021 acctcctcag ggaagtatgt catgatatcc tgcagacgcg tgagaattct cgaggaattc
     4081 tttgccaaaa tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa
     4141 gaagaaggca tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc
     4201 gaaccccgcc ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagg
     4261 ctggagaggg agaaggggac ggcggcgcgg cgacgcacga aggccctccc cgcccatttc
     4321 cttcctgccg gcgccgcacc gcttcgcccc gcgcccgcta gagggggtgc ggcggcgcct
     4381 cccagatttc ggctccgcac agatttggga caaaggaagt ccctgcgccc tctcgcacga
     4441 ttaccataaa aggcaatggc tgcggctcgc cgcgcctcga cagccgccgg cgctccgggg
     4501 gccgccgcgc ccctcccccg agcccycccc ggcccgaggc ggccccgccc cgcccggcac
     4561 ccccacctgc cgccaccccc cgcccggcac ggcgagcccc gcgccacgcc ccgtacggag
     4621 ccccgcaccc gaagccgggc cgtgctcagc aactcgggga ggggggtgca gggggggttg
     4681 cagcccgacc gacgcgccca caccccctgc tcaccccccc acgcacacac cccgcacgca
     4741 gcctttgttc ccctcgcagc cccccccgca ccgcggggca ccgcccccgg ccgcgctccc
     4801 ctcgcgcaca ctgcggagcg cacaaagccc cgcgccgcgc ccgcagcgct cacagccgcc
     4861 gggcagcgcg gagccgcacg cggcgctccc cacgcacaca cacacgcacg caccccccga
     4921 gccgctcccc ccgcacaaag ggccctcccg gagcccctca aggctttcac gcagccacag
     4981 aaaagaaaca agccgtcatt aaaccaagcg ctaattacag cccggaggag aagggccgtc
     5041 ccgcccgctc acctgtggga gtaacgcggt cagtcagagc cggggcgggc ggcgcgaggc
     5101 ggcggcggag cggggcacgg ggcgaaggca gcgcgcagcg actcccgccc gccgcgcgct
     5161 tcgcttttta tagggccgcc gccgccgccg cctcgccata aaaggaaact ttcggagcgc
     5221 gccgctctga ttggctgccg ccgcmcctcy ccsccycscc cccccccccc cccccccccc
     5281 cccccccccc cccggcgcgc cccccccccc cccccgcccc catcgctgca caaaataatt
     5341 aaaaaataaa taaatacaaa attgggggtg gggagggggg ggagatgggg agagtgaagc
     5401 agaacgtggg gctcacctcg accatggtaa tagcgatgac taatacgtag atgtactgcc
     5461 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     5521 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     5581 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     5641 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     5701 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     5761 ccgtaattga ttactattaa taactagtcg aagttcctat tctctagaaa gtataggaac
     5821 ttcaggtctg aagaggagtt tacgtccagc caagctagct tggctgcagg tcgtcgaaat
     5881 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     5941 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     6001 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     6061 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     6121 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     6181 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     6241 gaaggggtgg gtccgggggc ggggmrgggg kngggtccgg gggcggggcg ggcgcccgaa
     6301 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     6361 cctcttcctc atctccgggc ctttcgacct gcagcctgtt gacaattaat catcggcata
     6421 gtatatcggc atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc
     6481 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6541 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6601 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6661 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6721 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6781 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6841 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6901 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6961 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7021 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaggggatc aattctctag
     7081 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7141 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7201 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     7261 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7321 tatggcttct gaggcggaaa gaaccagctg gggctcgact agagcttgcg gaacccttcg
     7381 aagttcctat tctctagaaa gtataggaac ttcggcgcgc catttaaatt ttgggatgtt
     7441 ttctgtttgt tttgagatag ggtttttcag tgtagtcctg gctgtcctag aactcactct
     7501 gtagaccagg ctggcctgga actcaaggac tgccagcctc tgcctcctga gcatcgggat
     7561 taaaggtgtg tgccaccact gcccagcaga gtgcgttttt actcttcact ttgtgggtcc
     7621 tggctgcctg agattgtgtg cttgcaccat tagatgggag ggatgacttg ggaaagggtt
     7681 gagtcttttg tgagtcttgc ggaggcccag gagggggtac gtaaattaga gttctaagag
     7741 gcccagcaaa agtgaacaag tgactggcca ctgtgagttc actgggggac ctgagagtta
     7801 actagtgaaa aggagaggtc ctagtggaga agctaaagaa ttaaagttac tgccttgaag
     7861 ttctttgtga aggccaagct ctggagcgcg gcttgaaaag cctttatccc agagcagcat
     7921 gggaaggggc cccagctggg tgcgagcctg caaaccagag cacacctatc acagggcctt
     7981 ttcttaccag tgggacggga tcaaggcgga gcttacctgg gaacgtacag ggcggaacct
     8041 taacttgggg gcgtggccga atcacctggg tttggaaacg gttcttgtct ttcctcaagc
     8101 tcaggagagg ttgtttgcag gacactggcc cggaggggcc cgaactctcc gagggagggt
     8161 ttcgtggatc agaacaccgt cccttgacaa aaagatgcag agcaccgtct gcattctgct
     8221 agtaaccgtc cctcctgggg gcggagccag ccctcctccg ggtgtataaa gtagctgagc
     8281 gcctccttct gacaaacgcc tcacccccaa gagtactggg ggagtacgca gcacgccagc
     8341 ctaggggcgg agctcagccc tggaggaggc gtttcttctt agttctccga ctcacgcctc
     8401 tccacttttc atttaaatgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8461 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8521 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8641 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9241 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9661 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10561 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10621 taaaaatagg cgtatcacga ggccctttcg tc
//
