LOCUS       sequence-403309-       11170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) 
            and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, 
            Trinidad M, Ngo W, Doudna JA
  TITLE     In vivo human T cell engineering with enveloped delivery vehicles.
  JOURNAL   Nat Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z.
  PUBMED    38212493
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z."
COMMENT     Sequence Label: pJRH-1180 U6-reci Gag-pol v2
FEATURES             Location/Qualifiers
     source          1..11170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..332
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     84..104
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     255..274
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        491..870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        872..1149
                     /label=chicken beta-actin promoter
     intron          1150..2166
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2090..2112
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2174..2193
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2272..3774
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             3567..6578
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6263..6380
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7151..7384
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7569..7613
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7907..7933
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(8218..8237)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8283..8338
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8337..8356)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8699..8715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8699..8715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8712..8734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8723..8739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8747..8753,8754..8771,8772..8777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8792..8813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8872..9067
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8918..9053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9073..9207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9110..9129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9164..9183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9275..9292)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9446..10034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9526..9545)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10205..10996,10997..11065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10828..10847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11066..11170)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgggtc gaccgggttt attacaggga cagcagagat
       61 ccagtttggt taattaaggt accgagggcc tatttcccat gattccttca tatttgcata
      121 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      181 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      241 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      301 ctttatatat cttgtggaaa ggacgaaaca ccggagacgg aggacgacga acgtctctgt
      361 ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt
      421 gaaaaagtgg caccgagtcg gtgctttttt tgaattcgct agctaggtct tgaaaggagt
      481 gggaattgtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      541 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      721 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      781 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      841 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      901 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      961 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc
     1021 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1081 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1141 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1201 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1261 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     1321 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     1381 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     1441 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     1501 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     1561 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     1621 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     1681 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     1741 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     1801 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     1861 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     1921 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     1981 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2041 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2101 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2161 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc
     2221 gggccgcgtt gacgcgcacg gcaagaggcg aggggcggcg actggtgaga gatgggtgcg
     2281 agagcgtcag tattaagcgg gggagaatta gatcgatggg aaaaaattcg gttaaggcca
     2341 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     2401 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     2461 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     2521 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     2581 atagaggaag agcaaaacaa aagtaagaaa aaagcacagc aagcagcagc tgacacagga
     2641 cacagcaatc aggtcagcca aaattaccct atagtgcaga acatccaggg gcaaatggta
     2701 catcaggcca tatcacctag aactttaaat gcatgggtaa aagtagtaga agagaaggct
     2761 ttcagcccag aagtgatacc catgttttca gcattatcag aaggagccac cccacaagat
     2821 ttaaacacca tgctaaacac agtgggggga catcaagcag ccatgcaaat gttaaaagag
     2881 accatcaatg aggaagctgc agaatgggat agagtgcatc cagtgcatgc agggcctatt
     2941 gcaccaggcc agatgagaga accaagggga agtgacatag caggaactac tagtaccctt
     3001 caggaacaaa taggatggat gacacataat ccacctatcc cagtaggaga aatctataaa
     3061 agatggataa tcctgggatt aaataaaata gtaagaatgt atagccctac cagcattctg
     3121 gacataagac aaggaccaaa ggaacccttt agagactatg tagaccgatt ctataaaact
     3181 ctaagagccg agcaagcttc acaagaggta aaaaattgga tgacagaaac cttgttggtc
     3241 caaaatgcga acccagattg taagactatt ttaaaagcat tgggaccagg agcgacacta
     3301 gaagaaatga tgacagcatg tcagggagtg gggggacccg gccataaagc aagagttttg
     3361 gctgaagcaa tgagccaagt aacaaatcca gctaccataa tgatacagaa aggcaatttt
     3421 aggaaccaaa gaaagactgt taagtgtttc aattgtggca aagaagggca catagccaaa
     3481 aattgcaggg cccctaggaa aaagggctgt tggaaatgtg gaaaggaagg acaccaaatg
     3541 aaagattgta ctgagagaca ggctaatttt ttagggaaga tctggccttc ccacaaggga
     3601 aggccaggga attttcttca gagcagacca gagccaacag ccccaccaga agagagcttc
     3661 aggtttgggg aagagacaac aactccctct cagaagcagg agccgataga caaggaactg
     3721 tatcctttag cttccctcag atcactcttt ggcagcgacc cctcgtcaca ataaagatag
     3781 gggggcaatt aaaggaagct ctattagata caggagcaga tgatacagta ttagaagaaa
     3841 tgaatttgcc aggaagatgg aaaccaaaaa tgataggggg aattggaggt tttatcaaag
     3901 taagacagta tgatcagata ctcatagaaa tctgcggaca taaagctata ggtacagtat
     3961 tagtaggacc tacacctgtc aacataattg gaagaaatct gttgactcag attggctgca
     4021 ctttaaattt tcccattagt cctattgaga ctgtaccagt aaaattaaag ccaggaatgg
     4081 atggcccaaa agttaaacaa tggccattga cagaagaaaa aataaaagca ttagtagaaa
     4141 tttgtacaga aatggaaaag gaaggaaaaa tttcaaaaat tgggcctgaa aatccataca
     4201 atactccagt atttgccata aagaaaaaag acagtactaa atggagaaaa ttagtagatt
     4261 tcagagaact taataagaga actcaagatt tctgggaagt tcaattagga ataccacatc
     4321 ctgcagggtt aaaacagaaa aaatcagtaa cagtactgga tgtgggcgat gcatattttt
     4381 cagttccctt agataaagac ttcaggaagt atactgcatt taccatacct agtataaaca
     4441 atgagacacc agggattaga tatcagtaca atgtgcttcc acagggatgg aaaggatcac
     4501 cagcaatatt ccagtgtagc atgacaaaaa tcttagagcc ttttagaaaa caaaatccag
     4561 acatagtcat ctatcaatac atggatgatt tgtatgtagg atctgactta gaaatagggc
     4621 agcatagaac aaaaatagag gaactgagac aacatctgtt gaggtgggga tttaccacac
     4681 cagacaaaaa acatcagaaa gaacctccat tcctttggat gggttatgaa ctccatcctg
     4741 ataaatggac agtacagcct atagtgctgc cagaaaagga cagctggact gtcaatgaca
     4801 tacagaaatt agtgggaaaa ttgaattggg caagtcagat ttatgcaggg attaaagtaa
     4861 ggcaattatg taaacttctt aggggaacca aagcactaac agaagtagta ccactaacag
     4921 aagaagcaga gctagaactg gcagaaaaca gggagattct aaaagaaccg gtacatggag
     4981 tgtattatga cccatcaaaa gacttaatag cagaaataca gaagcagggg caaggccaat
     5041 ggacatatca aatttatcaa gagccattta aaaatctgaa aacaggaaag tatgcaagaa
     5101 tgaagggtgc ccacactaat gatgtgaaac aattaacaga ggcagtacaa aaaatagcca
     5161 cagaaagcat agtaatatgg ggaaagactc ctaaatttaa attacccata caaaaggaaa
     5221 catgggaagc atggtggaca gagtattggc aagccacctg gattcctgag tgggagtttg
     5281 tcaatacccc tcccttagtg aagttatggt accagttaga gaaagaaccc ataataggag
     5341 cagaaacttt ctatgtagat ggggcagcca atagggaaac taaattagga aaagcaggat
     5401 atgtaactga cagaggaaga caaaaagttg tccccctaac ggacacaaca aatcagaaga
     5461 ctgagttaca agcaattcat ctagctttgc aggattcggg attagaagta aacatagtga
     5521 cagactcaca atatgcattg ggaatcattc aagcacaacc agataagagt gaatcagagt
     5581 tagtcagtca aataatagag cagttaataa aaaaggaaaa agtctacctg gcatgggtac
     5641 cagcacacaa aggaattgga ggaaatgaac aagtagataa attggtcagt gctggaatca
     5701 ggaaagtact atttttagat ggaatagata aggcccaaga agaacatgag aaatatcaca
     5761 gtaattggag agcaatggct agtgatttta acctaccacc tgtagtagca aaagaaatag
     5821 tagccagctg tgataaatgt cagctaaaag gggaagccat gcatggacaa gtagactgta
     5881 gcccaggaat atggcagcta gattgtacac atttagaagg aaaagttatc ttggtagcag
     5941 ttcatgtagc cagtggatat atagaagcag aagtaattcc agcagagaca gggcaagaaa
     6001 cagcatactt cctcttaaaa ttagcaggaa gatggccagt aaaaacagta catacagaca
     6061 atggcagcaa tttcaccagt actacagtta aggccgcctg ttggtgggcg gggatcaagc
     6121 aggaatttgg cattccctac aatccccaaa gtcaaggagt aatagaatct atgaataaag
     6181 aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag acagcagtac
     6241 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     6301 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     6361 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggaaag
     6421 gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat aatagtgaca
     6481 taaaagtagt gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa cagatggcag
     6541 gtgatgattg tgtggcaagt agacaggatg aggattaaca catggaattc tgcaacaact
     6601 gctgtttatc catttcagaa ttgggttcga catagcagaa taggcgttac tcgacagagg
     6661 agagcaagaa atggagccag tagatcctag actagagccc tggaagcatc caggaagtca
     6721 gcctaaaact gcttgtacca attgctattg taaaaagtgt tgctttcatt gccaagtttg
     6781 tttcatgaca aaagccttag gcatctccta tggcaggaag aagcggagac agcgacgaag
     6841 agctcatcag aacagtcaga ctcatcaagc ttctctatca aagcagtaag tagtacatgt
     6901 aatgcaacct ataatagtag caatagtagc attagtagta gcaataataa tagcaatagt
     6961 tgtgtggtcc atagtaatca tagaatatag gaaaatggcc gctgatcttc agacctggag
     7021 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     7081 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     7141 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     7201 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     7261 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     7321 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     7381 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     7441 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     7501 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     7561 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7621 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7681 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7741 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     7801 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     7861 ccttggcact tatctgggac gatctgcgga gcctgtgcct cttcagctac caccgcttga
     7921 gagacttact cttgattgta acgaggattg tggaacttct gggacgcagg gggtgggaag
     7981 ccctcaaata ttggtggaat ctcctacaat attggagtca ggagctaaag aatagtgctg
     8041 ttagcttgct caatgccaca gccatagcag tagctgaggg gacagatagg gttatagaag
     8101 tagtacaagg agcttgtaga gctattcgcc acatacctag aagaataaga cagggcttgg
     8161 aaaggatttt gctataagct cgaaacaacc ggtacctcta gaactatagc tagcagatct
     8221 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     8281 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     8341 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     8401 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     8461 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     8521 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     8581 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     8641 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     8701 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8761 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8821 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
     8881 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8941 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9001 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9061 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9121 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9181 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
     9241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11161 gggttccgcg
//
