LOCUS       sequence-403317-        3578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Bxb1 excisionase fused with mScarlet for fluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Pandey A, Rodriguez ML, Poole W, Murray RM
  TITLE     Characterization of Integrase and Excisionase Activity in a 
            Cell-Free Protein Expression System Using a Modeling and Analysis 
            Pipeline.
  JOURNAL   ACS Synth Biol. 2023 Feb 17;12(2):511-523. doi: 
            10.1021/acssynbio.2c00534. Epub 2023 Jan 30.
  PUBMED    36715625
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00534"; journalName: "ACS Synth Biol"; date: 
            "2023-02-17- 17"; volume: "12"; issue: "2"; pages: "511-523"
COMMENT     Sequence Label: pTet-Bxb1-Xis-mScarlet
FEATURES             Location/Qualifiers
     source          1..3578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    215..233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    240..258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             343..351
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        2010..2112
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2113..2772
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2179..2198)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(3121..3578,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctgtggaa aaacgccagc aacgcggcct ttttacggtt cctggcggag cattactcgc
      181 atccattctc aggctgtctc gtctcgtctc ggagtcccta tcagtgatag agattgacat
      241 ccctatcagt gatagagata ctgagcacta ctgggcccaa gttcacttaa aaaggagatc
      301 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
      361 gccggcactc agcgtatcgt ctttctaccc gatactcagt tgcctttcga ggcgcgcaaa
      421 gagatgcaag cggtcatccg cttcatcggg gatgtccagc cgtacggcgt ggtacatatc
      481 ggtgacgtcc tagacctgcc gcagccctcg cgctggaacc ggggaaccaa gggcgagttc
      541 gagggctcgg tgtaccgcga cgcggactac gccaagaaga acctgatgga gccgctgcgc
      601 aaggtctacg acggctggat cgggatgcac gagggcaacc acgatctgcg agcccgcgag
      661 tacctggcca agaacgcacc ggccctggag ggtacgcacg ctttcgacat cgacgtgctg
      721 ctcgacttcg acgggttcgg tgtggagctg ctgcctgact tctacgacat cgctccgggc
      781 tggatctcca ctcacgggca catgggcaag atgacgctat cccagatcgc cggatcgaca
      841 gcgctcaacg gtgccaagaa gttcggcaag tccgtggtct gcggccacac gcaccggcag
      901 gctgtcgtct cgcactcgtt cgggtacggc ggctcggtgc gcaagaccgt caccggcatg
      961 gaagtcgggc acctgatgga catgaagaag gccaactatc taaagggcgg agccgggaac
     1021 tggcagatgg gcttcgggat gctcacggtc gacggcaagc atgtcaaggc tgagatcgtc
     1081 ccgatcctgg gaggcaagtt caccgttgac ggccaggtct gggaagtcac cggtggaagc
     1141 ggaagtggat ccggtagtgg cagcggcagc atggtcagta aaggcgaagc agttatcaaa
     1201 gagttcatgc gcttcaaagt tcatatggaa gggtcgatga acgggcacga atttgaaatt
     1261 gaaggcgaag gcgaaggccg cccatatgaa gggacccaaa ccgcaaagct taaggttact
     1321 aaaggcggtc cattaccctt ttcgtgggac attttaagcc cacagtttat gtacgggagt
     1381 cgcgctttca tcaagcaccc tgcggacatc ccagattact acaaacagtc tttccccgag
     1441 gggttcaagt gggagcgcgt gatgaacttc gaggatggcg gagccgtgac ggtcacccaa
     1501 gatacctctt tggaggacgg tacgttgatc tacaaagtga aattgcgtgg cacgaatttt
     1561 ccacctgatg ggcctgtcat gcagaaaaag acaatgggat gggaagcttc cacggagcgc
     1621 ctttacccag aggacggtgt tcttaaaggg gatatcaaaa tggcgctgcg tcttaaagat
     1681 ggaggccgct acctggcgga cttcaagact acttacaagg ccaaaaaacc agtgcagatg
     1741 ccgggtgcgt acaatgtaga tcgtaaatta gatattacaa gtcacaatga agattacacg
     1801 gtcgtagagc agtatgagcg cagtgagggg cgtcactcta cgggcggtat ggacgagtta
     1861 tacaagtaaa ggtactcaaa gcccgccgaa aggcgggctt ttctgtcctt gagagcttcc
     1921 aggatacata gattaccaca actccgagcc cttccaccgc ttgttgatac cgggaagccc
     1981 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     2041 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     2101 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     2161 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagact
     2221 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     2281 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     2341 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     2401 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     2461 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     2521 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     2581 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     2641 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     2701 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     2761 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
     2821 tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc gacccggtcg
     2881 tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt accggtttat
     2941 tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag tttgctcagg
     3001 ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc cagagcctga
     3061 taaaaacggt tagcgcttcg tcgctgcgtc agaccccgta gaaaagatca aaggatcttc
     3121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcg
//
