LOCUS       sequence-403336-       18896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Frt-inducible CRISPRi by Zim3-dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18896)
  TITLE     CRISPRi by Zim3-dCas9 for iPSC Neurodegenerative Disease Initiative 
            (iNDI)
REFERENCE   2  (bases 1 to 18896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP
FEATURES             Location/Qualifiers
     source          1..18896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   426..488
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(618..635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(618..634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(627..649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      776..1231
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(863..882)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1073..1094
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1257..1361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1362..1430,1431..2222)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1580..1599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2393..2981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2882..2901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3135..3152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3269..3290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3305..3310,3311..3328,3329..3335)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3343..3359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3348..3370
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3367..3383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3367..3383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   3874..3908
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    4424..4457
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4458..4682
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4702..5412)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      5409..5418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5440..5466)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(5482..5508)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(5515..5568)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5578..6174)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5658..5678)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6155..6174
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          6197..7135
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6220..6240)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(7136..7347)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    7348..7381
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        9615..9994
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9996..10272
                     /label=chicken beta-actin promoter
     intron          10273..11289
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     11213..11235
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     11297..11316
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    11362..11395
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    11562..11696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    11816..11950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    12070..12204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    12233..12266
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             join(12690..12716,12717..12719,12720..15206,15207..15209,
                     15210..16793)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             16803..16829
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             16848..16868
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             16875..16895
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             16911..16964
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             16965..16991
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             17007..17033
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    17781..18369
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(17834..17854)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(18252..18263)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    18401..18877
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(18600..18619)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
ORIGIN
        1 attaaggccg tggaggcctt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca
       61 gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc
      121 gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc
      181 tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg
      241 gctgtccctc ataaaagttt tgttacttta tagaagaaat tttgagtttt tgtttttttt
      301 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
      361 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
      421 aaacacatgc gtcaatttta cacatgatta tctttaacgt acgtcacaat atgattatct
      481 ttctagggtt aatctagctg cgtgttctgc agcgtgtcga gcatcttcat ctgctccatc
      541 acgctgtaaa acacatttgc accgcgagtc tgcccgtcct ccacgggttc aaaaacgtga
      601 atgaacgagg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      661 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      721 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
      781 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      841 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      901 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      961 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1021 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1081 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     1141 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     1201 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     1261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1381 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1441 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1501 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1561 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1621 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1681 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1741 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1801 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1861 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1921 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2221 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2521 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3061 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3181 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3241 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     3301 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     3361 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcccgccgg gtaactcacg
     3421 gggtatccat gtccatttct gcggcatcca gccaggatac ccgtcctcgc tgacgtaata
     3481 tcccagcgcc gcaccgctgt cattaatctg cacaccggca cggcagttcc ggctgtcgcc
     3541 ggtattgttc gggttgctga tgcgcttcgg gctgaccatc cggaactgtg tccggaaaag
     3601 ccgcgacgaa ctggtatccc aggtggcctg aacgaacagt tcaccgttaa aggcgtgcat
     3661 ggccacacct tcccgaatca tcatggtaaa cgtgcgtttt cgctcaacgt caatgcagca
     3721 gcagtcatcc tcggcaaact ctttccatgc cgcttcaacc tcgcgggaaa aggcacgggc
     3781 ttcttcctcc ccgatgccca gatagcgcca gcttgggcga tgactgagcc ggaaaaaaga
     3841 cccgacgata tgatcctgat gcagctagat taaccctaga aagatagtct gcgtaaaatt
     3901 gacgcatgca ttcttgaaat attgctctct ctttctaaat agcgcgaatc cgtcgctgtg
     3961 catttaggac atctcagtcg ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta
     4021 agtgtcactg attttgaact ataacgaccg cgtgagtcaa aatgacgcat gattatcttt
     4081 tacgtgactt ttaagattta actcatacga taattatatt gttatttcat gttctactta
     4141 cgtgataact tattatatat atattttctt gttatagaat cagggacagc ccccccccaa
     4201 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     4261 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     4321 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     4381 gcctgcagac acctgggggg atacggggaa aaagccatac caaataactt cgtataatgt
     4441 atgctatacg aagttatcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
     4501 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     4561 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     4621 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     4681 agaattctta gcggccgcct aattaagctt gtgccccagt ttgctaggga ggtcgcagta
     4741 tctggccact gccacctcgt gctgctcgac gtaggtctcg ttgttggcct ccttgattct
     4801 ttccagtctg tagtccacat agtagacgcc aggcatcttg aggttcttag cgggtttctt
     4861 ggatctatat gtggtcttgg cgtttgcgat cagatggctc ccgcccacga gcttcagggc
     4921 catgtcgttt ctgccttcca ggccgccgtc agcggggtac agcgtctcgg tgaaggcctc
     4981 ccagccgagt gttttcttct gcatcacagg gccgttggat gtgaagttca cccctctgat
     5041 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac
     5101 gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt
     5161 gaagaagtcg gggatgccct gggtgtggtt gatgaaggtc ttgctgccgt agaggaagct
     5221 agtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat
     5281 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtgatggtt
     5341 gtccacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcttcgcc
     5401 cttagacacc atggtggcgg tggctccccc tgaccctcca tccaacttaa cgcgctttgc
     5461 agcaggacct ccagaaccgc cgtcgagttt gacccgttta gcggcaggga ccggtgggcc
     5521 aggattctcc tcgacgtcac cgcatgttag cagacttcct ctgccctctc ccgatccggc
     5581 accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt
     5641 gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa
     5701 ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag
     5761 acccttgccc tggtggtcgg gcgagactcc gacggtggcc aggaaccacg cgggctcctt
     5821 gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct
     5881 caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg
     5941 cgtggtccag accgccacgg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag
     6001 cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg
     6061 atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc
     6121 cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggtggg
     6181 atttaaatca cgacacctga aatggaagaa aaaaactttg aaccactgtc tgaggcttga
     6241 gaatgaacca agatccaaac tcaaaaaggg caaattccaa ggagaattac atcaagtgcc
     6301 aagctggcct aacttcagtc tccacccact cagtgtgggg aaactccatc gcataaaacc
     6361 cctcccccca acctaaagac gacgtactcc aaaagcgcga gaactaatcg aggtgcctgg
     6421 acggcgcccg gtactccgtg gagtcacatg aagcgacggc tgaggacgga aaggcccttt
     6481 tcctttgtgt gggtgactca cccgcccgct ctcccgagcg ccgcgtcctc cattttgagc
     6541 tccctgcagc agggccggga agcggccatc tttccgctca cgcaactggt gccgaccggg
     6601 ccagccttgc cgcccagggc ggggcgatac acggcggcgc gaggccaggc accagagcag
     6661 gccggccagc ttgagactac ccccgtccga ttctcggtgg ccgcgctcgc aggccccgcc
     6721 tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc gcccgcggcc ccaaaaaccg
     6781 aaataccagt gtgcagatct tggcccgcat ttacaagact atcttgccag aaaaaaagcg
     6841 tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca gcgagacagg
     6901 cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg cccaggccag gcctcaactc
     6961 aagcacgagg cgaaggggct ccttaagcgc aaggcctcga actctcccac ccacttccaa
     7021 cccgaagctc gggatcaaga atcacgtact gcagccaggt ggaagtaatt caaggcacgc
     7081 aagggccata acccgtaaag aggccaggcc cgcgggaacc acacacggca cttacctgtg
     7141 ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg
     7201 gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta
     7261 ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg
     7321 ggactgtggg cgatgtgcgc tctgcccata acttcgtata atgtatgcta tacgaagtta
     7381 taatattctc actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc
     7441 aggggagggt cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg
     7501 gcacagctgc ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg
     7561 gatgtggcac tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg
     7621 gatcttggag ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca
     7681 attctccagc tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt
     7741 ccaccccttc tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag
     7801 cagtgtctgt ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct
     7861 tctatcctat cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca
     7921 gagccctcaa cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga
     7981 tctgcctctc cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca
     8041 cagcaaactc ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt
     8101 tgggttggat atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg
     8161 cccaaggagg ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct
     8221 ctgggcagcc tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg
     8281 agcactgtgc tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt
     8341 tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     8401 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     8461 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     8521 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     8581 ccccgtgagc tccacgtgcc tagggcacac gaccacaatt ccactgaaag cattttaata
     8641 cggaacttgt cactcccagg gagcctccgc tcagccggca gttggttcat ttcaatcccc
     8701 acgacaaccc ttcaaagtgc agggcagaca gcaggtggct ctgcccaggc gcctggatca
     8761 cagcccggcc tgcagccctc acctgggcgc ggggagaccc tgaggacgct cctccaggcg
     8821 gcgctggccg gggcctgcgg acacggacgg gcgggctgag ctccgggacc cctccccgcg
     8881 ccccgcaccc cgcaccccgc accccgcacc ccgcacccgg cgctcacccg tcccagcccc
     8941 gccgcccgca gccccagctg caacgcagcc accgccgcca tcgcacccgg ccccgcgggc
     9001 gcttccggga cgcaggaggc atctgcatcc ggggcgccgc tgagtcccgc ccagagcccc
     9061 gcccccggct ccaggttctg cgagcggctt ccgccgggct gctccgcggg cgcgtcggcc
     9121 atgagcgagt tgccgggcga cgtgcgggcg tttctgcggg agcacccgag cctgcggctc
     9181 cagacggacg cccgcaaggt tcgcagcgcg ggaggggaac ggagtggcgg agaagggcgc
     9241 agttgggatg aggggctgag gggagggcag gggagaggag agggcagggg agaggggaga
     9301 ggggagagca ggagagaggg gaaggcaggg gagagggcgc ggcgggatca ggggaggaga
     9361 gggaaggggg cgcggcagga gggggcacca gggagcggag ccctggccct cctgacgtcc
     9421 tgcccgccca cgcgtccgca ggtgaggtgc atcctgacag gtcacgagct gccctgccgc
     9481 ctgccggagc tccaggtcta cacccgcggc aaaaagtacc agcggctggt ccgcgcctcc
     9541 ccggccttcg actatgcaga gttcgagccg cacatcgtgc ccagcaccaa gaacccgtag
     9601 gtggtcggcg cgccgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     9661 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     9721 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     9781 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     9841 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     9901 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     9961 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
    10021 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
    10081 attttgtgca gcgatggggg cggggggggg ggggggcgcg cgccaggcgg ggcggggcgg
    10141 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
    10201 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
    10261 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
    10321 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
    10381 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
    10441 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
    10501 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
    10561 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
    10621 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
    10681 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
    10741 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
    10801 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
    10861 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc
    10921 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
    10981 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
    11041 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
    11101 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
    11161 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
    11221 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
    11281 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
    11341 tggtaccgtt aacgcgatcg cgaagttcct attctctaga aagtatagga acttcccgcg
    11401 atgaataaat gaaagcttgc agatctgcga ccctagagga tctgcgaccc tagaggatca
    11461 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    11521 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    11581 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11641 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgcg
    11701 accctagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    11761 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    11821 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    11881 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    11941 tgtctggatc tgcgacccta gaggatcata atcagccata ccacatttgt agaggtttta
    12001 cttgctttaa aaaacctccc acacctcccc ctgaacctga aactaaaaat gaatgcaatt
    12061 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    12121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    12181 aatgtatctt atcatgtctg gatctccatc aagctgatcc ggaaccctta atgaagttcc
    12241 tattctctag aaagtatagg aacttcgtat actcgcgagc cgccaccatg aacaactctc
    12301 agggcagagt gaccttcgag gacgtgaccg tgaacttcac ccaaggcgag tggcagagac
    12361 tgaaccccga gcagagaaac ctgtaccggg acgtgatgct ggaaaactac agcaacctgg
    12421 tgtccgtcgg ccagggcgag acaacaaagc ctgacgtgat cctgcggctg gaacagggca
    12481 aagagccttg gctggaagag gaagaggtgc tcggatctgg cagagccgag aagaatggcg
    12541 acatcggcgg acagatctgg aagcccaagg acgtgaaaga gtctctgagt ggcggctcaa
    12601 gtggcgggtc tagtgggagt gaaacgccag ggacttcaga atcagcaacc ccggaatcat
    12661 ccggcggatc atccggtggg tctgtataca tggacaagaa gtactctatc ggcctggcca
    12721 tcggcaccaa ttctgttgga tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
    12781 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaatctg atcggcgccc
    12841 tgctgttcga ctctggcgaa acagccgaag ccaccagact gaagagaacc gccagacggc
    12901 ggtacaccag aagaaagaac cggatctgct acctgcaaga gatcttcagc aacgagatgg
    12961 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtc gaggaagata
    13021 agaagcacga gcggcacccc atcttcggca acatcgtgga tgaggtggcc taccacgaga
    13081 agtaccctac catctaccac ctccggaaga aactggtgga cagcaccgac aaggccgacc
    13141 tgagactgat ctatctggcc ctggctcaca tgatcaagtt ccggggccac tttctgatcg
    13201 agggcgacct gaatcctgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
    13261 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggagtggat gccaaggcca
    13321 tcctgtctgc cagactgagc aagagcagac gcctggaaaa cctgatcgct cagctgcccg
    13381 gcgagaagaa gaacggcctg ttcggaaacc tgattgccct gagcctgggc ctgacaccta
    13441 acttcaagag caacttcgac ctggctgagg acgccaaact gcagctgtcc aaggacacct
    13501 acgacgacga cctggacaat ctgctggccc agatcggcga tcagtacgcc gacttgtttc
    13561 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
    13621 agatcacaaa ggcccctctg agcgcctcta tgatcaagag atacgacgag caccaccagg
    13681 atctgaccct gctgaaggcc ctcgttagac agcagctgcc tgagaagtac aaagagattt
    13741 tcttcgacca gagcaagaac ggctacgccg gctacattga tggcggagcc agccaagagg
    13801 aattttacaa gttcatcaag cccatccttg agaagatgga cggcaccgag gaactgctgg
    13861 tcaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaat ggctctatcc
    13921 ctcaccagat ccacctggga gagctgcacg ccattctgcg gagacaagag gacttttacc
    13981 cattcctgaa ggacaaccgg gaaaagattg agaagatcct gaccttcaga atcccctact
    14041 acgtgggacc actggccaga ggcaatagca gattcgcctg gatgaccaga aagagcgagg
    14101 aaaccatcac accctggaac ttcgaggaag tggtggacaa gggcgccagc gctcagtcct
    14161 tcatcgagcg gatgaccaac ttcgataaga acctgcctaa cgagaaggtg ctgcccaagc
    14221 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
    14281 ccgagggaat gagaaagccc gcctttctga gcggcgagca gaaaaaggcc attgtggatc
    14341 tgctgttcaa gaccaaccgg aaagtgacag tgaagcagct gaaagaggac tacttcaaga
    14401 aaatcgagtg cttcgacagc gtggaaatca gcggcgtgga agatcggttc aatgccagcc
    14461 tgggcacata ccacgacctg ctgaaaatta tcaaggacaa ggacttcctg gacaacgaag
    14521 agaacgagga catcctggaa gacatcgtgc tgaccctgac actgtttgag gacagagaga
    14581 tgatcgagga acggctgaaa acatacgccc acctgttcga cgacaaagtg atgaagcaac
    14641 tgaagcggcg gagatacacc ggctggggta gactgtcccg gaagctgatc aacggcatcc
    14701 gggataagca gtccggcaag accatcctgg actttctgaa gtccgacggc ttcgccaaca
    14761 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
    14821 cccaggtgtc cggccaaggc gattctctgc acgagcacat tgccaacctg gccggatctc
    14881 ccgccattaa gaagggcatc ctgcagaccg tgaaggtggt ggacgagctt gtgaaagtga
    14941 tgggcagaca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccacac
    15001 agaagggcca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
    15061 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
    15121 tgtacctgta ctacctgcag aatggacggg atatgtacgt ggaccaagag ctggacatca
    15181 accggctgtc cgactacgat gtggacgcta tcgtgcccca gtcttttctg aaggacgact
    15241 ccatcgacaa caaggtcctg accagaagcg acaagaaccg gggcaagagc gataacgtgc
    15301 cctccgaaga ggtggtcaag aagatgaaga actattggcg ccagctgctg aatgccaagc
    15361 tgattaccca gcggaagttc gacaacctga ccaaggccga aagaggcggc ctgagcgaac
    15421 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc accaaacacg
    15481 tggcacagat tctggactcc cggatgaaca ctaagtacga cgagaacgac aaactgatcc
    15541 gggaagtgaa agtcatcacc ctgaagtcca agctggtgtc tgatttccgg aaggatttcc
    15601 agttctacaa agtgcgcgag atcaacaact accatcacgc ccacgacgcc tacctgaatg
    15661 ccgttgttgg aacagccctg atcaagaagt atcccaagct ggaaagcgag ttcgtgtacg
    15721 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcaa gagattggca
    15781 aggctaccgc caagtacttt ttctactcca acatcatgaa ctttttcaag acagagatca
    15841 ccctggccaa cggcgagatc cggaaaagac ccctgatcga gacaaacggc gaaaccgggg
    15901 agatcgtgtg ggataagggc agagattttg ccacagtgcg gaaagtgctg agcatgcccc
    15961 aagtgaatat cgtgaagaaa accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
    16021 tgcctaagcg gaacagcgac aagctgatcg cccggaagaa ggactgggac ccaaagaagt
    16081 acggcggctt cgatagccct accgtggcct attctgtgct ggtggtggcc aaagtggaaa
    16141 agggcaagtc caagaaactc aagagcgtga aagaactgct cggcatcacc atcatggaac
    16201 ggtccagctt tgagaagaac cctattgatt tccttgaggc caagggctac aaagaagtga
    16261 aaaaggacct gatcatcaag ctccccaagt actccctgtt cgagctggaa aatggccgga
    16321 agcggatgct ggcttctgct ggcgaactgc agaagggaaa cgaactggcc ctgcctagca
    16381 aatatgtgaa ctttctgtac ctggccagcc actatgagaa gctgaagggc agccccgagg
    16441 acaatgagca aaagcagctg tttgtcgagc agcacaagca ctacctggac gagatcatcg
    16501 agcagatcag cgagttctcc aagagagtga tcctggctga cgccaatctg gacaaagtgc
    16561 tgtccgccta caacaagcac cgggacaagc ctatcagaga gcaggccgag aatatcatcc
    16621 acctgtttac cctgaccaac ctgggagccc ctgccgcctt caagtacttc gacaccacca
    16681 tcgaccggaa gcgctacacc agcaccaaag aggttctgga cgccacactg atccaccaga
    16741 gcatcaccgg cctgtacgag acaagaatcg acctgtctca gctcggcggc gatcctcgag
    16801 gctatcccta tgacgtgccc gattatgcca gcagtggcag cggctccccc aagaaaaaac
    16861 gcaaggtgga agatcctaag aaaaagcgga aagtggttaa cggaagtggc gagggcagag
    16921 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccacctgcc gctaaacggg
    16981 tcaaactcga cggcggttct ggaggtcctg ctgcaaagcg cgttaagttg gatgctagca
    17041 tggaatttgt gtctaaaggg gaagcagtca ttaaagagtt catgcgcttc aaagtacaca
    17101 tggaaggaag tatgaacggg cacgaatttg aaatagaagg cgaaggggaa ggtcggccat
    17161 acgaagggac gcaaaccgcg aagttgaagg ttacaaaggg aggaccgttg ccctttagct
    17221 gggacatcct gtctccacag tttatgtacg gttcaagagc ttttactaag caccccgctg
    17281 acatcccgga ctactataaa cagtcattcc ccgaaggatt caaatgggag agggtcatga
    17341 atttcgagga cggaggtgct gttacagtta cgcaggatac ctcacttgag gatggcacgc
    17401 ttatatataa ggtgaagctc cgcggtacga actttccgcc ggacggcccc gttatgcaga
    17461 agaagacgat gggctgggaa gcctctacgg aacgcctgta tcctgaggac ggtgttctga
    17521 aaggggatat taaaatggcc cttcggttga aagatggggg ccgctatctt gcggacttta
    17581 agaccaccta caaagcaaaa aagcctgtcc aaatgcccgg tgcatataac gtcgatagaa
    17641 aacttgatat aacctcccat aatgaagatt acacagtagt cgagcagtac gaacgaagcg
    17701 aaggtcggca ctcaactgga ggtatggatg aattgtataa gtaggatatc atgcctaatt
    17761 ttgagtgtat tactttcgaa aatcaacctc tggattacaa aatttgtgaa agattgactg
    17821 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    17881 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    17941 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    18001 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    18061 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    18121 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
    18181 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
    18241 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    18301 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    18361 cctccccgca tcgataccga gcgctggtcg acagatctac gggtggcatc cctgtgaccc
    18421 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
    18481 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
    18541 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
    18601 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
    18661 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
    18721 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
    18781 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
    18841 attacaggcg tgaaccactg ctcccttccc tgtccttgtt taaacgcgat cgctta
//
