LOCUS       sequence-403364-       11274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CRISPR-associated transposases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11274)
  TITLE     CRISPR-associated transposases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQCasTns(Ptr)-entry(BsaI)
FEATURES             Location/Qualifiers
     source          1..11274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(316..942)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     847..866
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        958..1013
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    964..982
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    994..1012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(9502..9520)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9516..9563
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(9763..10578)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9763..10578)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10486..10505
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(10579..10670)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(10688..11274,1..49))
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(10833..10852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgatcgtaa aaggatctca agaagatcct ttacggattc ccgacaccat cactctagat
       61 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
      121 gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
      181 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
      241 ttgcgctgat gaatccccta atgattttta tcaaaatcat taaggttacc atcacggaaa
      301 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
      361 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
      421 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
      481 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
      541 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
      601 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
      661 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
      721 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
      781 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
      841 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
      901 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
      961 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     1021 ctctagaaat aattttgttt aactttaaaa ggagatatac catgggtgaa ctgccgagta
     1081 ggcagctgga aatgagacct ctggtctcgt gaactgccga gtaggcagct gaagttggat
     1141 ccgaattcga gctcgggaag aaggagatat acatatgcac tttctggtgc agaccaagag
     1201 ctacccggac gaggcgctgg aaagctatct gctgcgtctg gcgcgtgata acagctacaa
     1261 cggttatagc gagctggcgg acatcctgtg gcagtggctg gcggaacaag ataacgagct
     1321 ggaaggtgcg ctgccgctgg cgctgagcaa ggtggacgtt taccacgcgc gtcaggcgag
     1381 cagcttccgt atccgtgcgc tgaaactggt ggcgcaactg gcggacgtta acgcgggtga
     1441 tattctggcg ctggcgtggc gtcgtagcaa cttcaagttt ggcaacctgg cggcggtgag
     1501 ccgtaacgag ctggcgatcc cgctggaact gctgcgtacc gataacatcc cggtttgcat
     1561 taaatgcctg agcgagagca gccacattcc gttttactgg cacctgaagc cgtataaagc
     1621 gtgccacaag cacaaaagcc agctgatcac ccgttgcaag gagtgctacg acctgattga
     1681 ttatcgtgcg agcgaggcgt ttctggaatg cgtttgcggt tgcaaaatca ccaacagcga
     1741 acaactgaac gacgcggatt tcaagatcgc gattgcgctg gcgagcagca acagccagaa
     1801 aatcgtgggc ctgattagct ggttcgcgaa ggtgaaacaa ctggacgtta gcgacgcgga
     1861 tttcaactgc gcgtttgttg attacttcaa cacctggccg gagagcctga ccaccgaact
     1921 ggacctgctg accaacaacg cgcgtctgaa gcagctgaac ccgtttaaca agaccaaatt
     1981 cagcagcgtg tacggtgacc tgatccgtga tggccaaatt gcggcgacca gcaaccgtaa
     2041 gaacaaagtt atcgacgaga tcattagcta ttttgtggaa ctggttgata gcaacccgaa
     2101 ggcgaaacac ccgaacattg gtgacctgct gctgtgcacc ttcgatgcgg cggtgctgct
     2161 gaacaccacc accgagcagg tttaccgtct gcaccaagaa gcgtttctga actgcgcgta
     2221 tagccagaag aaacacgaac aactgcgtgc ggatagccac gtgttctatc tgcgtcaggt
     2281 tatcgagctg cagcaagcgt ttgcggcgga aaaaccgctg accaagaaac aattcattgc
     2341 gccgtggtaa cttatgaacc tgcaggatgc gctggcgatt gagccgctga aggaaaaaac
     2401 caccgcgctg cgtaagctgt tcgtgccgta caccagccac gttgaggtgg atggttttga
     2461 ggaactggcg ctgaccgtgc tgatcaacct ggtttataag cgtagcgaaa ttgacgatct
     2521 gaccagcgcg cgtaccgcga aaagcgtgct gcgtgacgag gttctgctga gcaagtgcat
     2581 caacgaagtg aaatggttcc acacccacaa cctgaagtac ccggacatcc gtgttagcca
     2641 ccaacgtctg attagcgagg tggttagcga agatatcgcg ggtatttgca gccgtagcct
     2701 gccgctgagc tttggctgga gccacaacag cgcggagatc aaccacgcga aactgttcct
     2761 gaccagcttt aactggcagg gtgaagtgac ctgcctggcg cgtctgctga ttaacgagga
     2821 accggtttgg atcaacctga ttcgtgcgta cggtttcacc aagaaagcgg ttctggagat
     2881 cagcggcaag attaaacagc aactgccggt ggcggagttc ccgctggaag ttagcagctt
     2941 tagcccgcag ctgcaaatgc cgtttcagca aagctatctg gtggttaccc cggtggttag
     3001 ccacgcgatg ctggcgaaga tccagcaact gaccaccgac cgtaaactga acttcgcgct
     3061 ggttgagcac agccgtccgg cgaacgttgg tgatctggcg agcagcgtgg gtggcaacat
     3121 tcgtgttctg cgttactttc cgaagaccta tagcaaagcg gtgaaccgta gcaaagttgc
     3181 gaacaacgat atcgaaaagg cgttcaaaat tcgtgcgctg ctgagcagcc agtttcagca
     3241 agcgctgctg gtgctggttg gcatcaagca gttcaacacc ctgcgtcaaa aacgtctggc
     3301 gcgtgtggcg gcgatccgtc aagtgcgtgt tagcctgcaa ctgtggctgg acaacattct
     3361 ggaggcgaag aacaacgcgc agaaccaagt gtacccggaa tgggttcgtc actatctgga
     3421 tcaaagcatc accaactgca ttagccagtt cagcaacgtt ctgaacgaaa gcctgggtaa
     3481 cctgagcaag ctgaaacgtt ttgcgtacca cccgaacctg atgggcctgt tcaaagcgca
     3541 actgaactat gtgtttaccc actgcgcggc ggagcaggaa atcctgaacg acgagcaaat
     3601 tgtgtacgtt cactgccagg acatgcgtgt tttcgatgcg gaagcgatgg cgaacccgta
     3661 tatccagggt atgccgagcc tgaccgcgct gaacggcctg gcgcacaact tcgagcgtaa
     3721 gctgaaaaac tttattgatc cgagcatcaa gtgcattggt agcgcgatct acattgagaa
     3781 ctatcaactg cacaccggca aaccgctgcc ggaaccgagc aagctgaaac aggtggcggg
     3841 tcgtagccac gttatccgta gcggcatcat tgacaagccg aaatgcgaca ttaccctgga
     3901 tctggtgttc cgtctgtttg ttccgaacac cgaactgctg gataagctga acagccaact
     3961 gattaagccg gcgctgccga gcagctttgc gggtggcacc atgcacccgc cgagcctgta
     4021 ccagaacatt gactggtgcc acgtgcacac caagccgagc gagctgttta agaaactgaa
     4081 ggcgaaaagc agcaacggta gctggctgta tccgagcaag aaagtggtta aaagcttcga
     4141 acagctgatc gacgcgctga acagcaactt taacctgcgt ccggcggcga ttggcctggc
     4201 ggcgctggag gaaccggtga agcgtgatgc ggcgctgcac gagtaccact gctatgcgga
     4261 accggttatc ggtctgctgg agtgcgtgag caacaccagc gttaagtacg cgggcgcgaa
     4321 acaattcttt cacgacgcgt tctgggtgat ggatgttcag aaggaaagca tgctgatgaa
     4381 gaaaagcaaa tttgagtatg aataatgcag ctgccgcgtc acctgagcta cacccgtagc
     4441 ctgagcccga gcaaggcggt gttcttttat aaaaccccgg agagcgactt cgaaccgctg
     4501 cagatcgagc aaaacaaact ggtgggtcag aagagcggtt ttggcgatgc gtaccagaag
     4561 caaaacgttg cgaaaaacct ggcgccgcag gacctggcgt ttggtaaccc gcaaaccatt
     4621 gatgtgtgct atgttccgcc gaccgtgaac gaactgttct gccgttttag cctgcgtgtt
     4681 gaggcgaact gcatcgaacc gcacgtgtgc gacgatccga aggttattta ctggctgaaa
     4741 cgtttctttg aaacctataa gaaacacaac ggtctgaacg aagtggcgac ccgttacgcg
     4801 aagaacatcc tgatgggcaa ctggctgtgg cgtaaccgtc agagcccgaa cgttgacatc
     4861 gagattctga ccgaacacgc ggcgccgatt gtggttgagg gtgcgcagaa gctgaaatgg
     4921 caaggcaact ggcagaacaa ccaaaccgcg ctgctgaccc tgagcgagag catccaggaa
     4981 ggtctgagca acccgcaaaa ctactgctat ctggatatca ccgcgaagat taaaaacgcg
     5041 ttcagccagg aagtgcaccc gagccaaaag tttgtggaca acgttgaaca gggtatgagc
     5101 agcaaacagc tggcgtatac ccaagtgggc gataagaaag cggcgagcct gaacagccag
     5161 aaggttggcg cggcgatcca aaccattgac gattggtacg aggaaggtta taaaccgctg
     5221 cgtacccatg agtatggtgc ggacaagcaa atcctggtgg cgcaccgtac cccgaaaagc
     5281 cacagcgatt tttatagcct gctgccgcgt atcgcgctgc acattaagca catggaaaaa
     5341 cacggtctgg agcagagcga acaaagcaac agcatccact tcattgcggc ggttctgatt
     5401 aagggtggcc tgtttcagcg tagcaaagga tgaagcgtta ctatttcacc atcacctacc
     5461 tgccgcaaag ctgcgatgtg agcctgctgg cgggtcgttg catcggcatt ctgcacggtt
     5521 tcatgagcag ccgtgagatc agcaacattg gcgtgtgctt tccgaaatgg aacgagcaga
     5581 ccatcggtaa cgaactggcg tttgttagca ccaacaagaa acaactgacc aacctgagcc
     5641 agcaaagcta tttcgagatg atggcgcacg acaagctgtt tggcctgagc aaaattctgg
     5701 aagtgccggt taaccagagc gaagtgatgt tcgttcgtaa ccaaagcgtg gcgaaggcgt
     5761 ttgttggtga aaagcaacgt cgtctgaaac gtgcgaagaa acgtgcggag gcgcgtggcg
     5821 aagtgtacaa cccggagtat aagttcgaag cgaaagatat cggtcacttt cacagcattc
     5881 cggtgagcag caagggtaac ggccagagct acgttctgca catccaaaag aacgagaacg
     5941 cggaaagcat taaaaaccag ttcaacaact atggctttgc gaccaaccaa attttcctgg
     6001 gcaccgtgcc gagcctgaac accctgctgt aaaaggagat ataccatgta ccgtcgtaag
     6061 ctgaaatata gccgtgttaa gaacctgcac aaatttgcga gccagaagaa caaaagcacc
     6121 tgcctggtgg agagcagcct ggaattcgac gcgtgcttcc actttgagtt cagcccgccg
     6181 atcgcggcgt ttgaagcgca accgctgggt tacgagtatg aattcgataa ccgtatttgc
     6241 cgttacaccc cggactttct gctgacccac accgatggca cccagaagtt catcgaggtt
     6301 aagccgcaaa gcaaaattgc ggacgaggat tttcgtgcgc gtttcatcga aaagcaggcg
     6361 attgcgaaac aagacggtcg tgatctgatc ctggtgaccg acaagcagat tcgtgtttac
     6421 ccgaccctga acaacctgaa actgctgcac cgttatagcg gctttcagag cctgaccgag
     6481 ctgcaagcga gcgtgctgga actggttaag cagtacggta gcatcaaagt gggccaactg
     6541 attcgttatc tgaaagttac cgcgggtgaa ctgctggcga ccgtgctgcg tctgctgagc
     6601 ctgggccaac tgttcgcgga tctgaccacc aacgagatca gcattgaaac cgcgatctgg
     6661 agcaacaatg tttaataacg acctgttcga cgatgagttt aaccagccgc tgccgaaggc
     6721 ggaaaccaaa ctgccgcaga actataccaa ggatctgcaa gcgctgccgg agaagatcaa
     6781 aaccaccacc ttcgcgaagc tgaaatacat tcaatggctg gaggcgaaca tccagggtgg
     6841 ctggacccaa aagaacctgg aaccgctgct gaaactgatg ccggacgttg agggtgaaaa
     6901 gaaaccgagc tggcgtaccg cggcgcgttg gtatagcgcg tacaccaacg cggataagaa
     6961 cattatggcg ctgatcccga gccaccagaa gaaaggcaac cgtgaacgtg acaccaccac
     7021 cgataagttc tttgagaaag cgctggaacg ttacctggtg aaggagaaac cgagcgttgc
     7081 gagcgcgtat aagttctaca aagacctggt gatcattgaa aacgacagcg tggttgatag
     7141 cgttctgaaa ccgctgacct ataaggcgtt taaaaaccgt attgacaacc tgccgcagta
     7201 tgaggttatg atcgcgcgtt acggcaagcg tctggcggat attgcgtaca acaaggtgga
     7261 aggccacaaa cgtccgattc gtgtgctgga gaaagttgaa atcgaccaca ccccgctgga
     7321 tctgattctg ctggacgatg agctgcacat cccgctgggt cgtccgaccc tgaccatgct
     7381 ggttgacgtt tatagccact gcatcgtggg ctactatttc agctttagcg agccgagcta
     7441 cgatgcggtt cgtcgtgcga tgctgaacgc gatgaagccg aaaagcgaag tggcgaaact
     7501 gtacccggac accattaacg agtggaagtg cgcgggtaaa atcgaaaccc tggtggttga
     7561 taacggcgcg gagttctgga gcaacagcct ggaactggcg tgcgaggaaa tcggtattaa
     7621 cacccagtat aacccggtgg cgaagccgtg gctgaaaccg ttcgttgagc gtatgtttgg
     7681 caccatcaac accgaactgc tggacccggt tccgggcaag accttcagca acatcctgca
     7741 aaaacacgaa tacaacccga agaaagacgc gattatgcgt ttcaccacct ttatgcagct
     7801 gtttcacaag tgggtggttg atgtgtatca ccaagacgcg gatagccgtt tcaaatacat
     7861 tccgagccag ctgtgggacc aaggctttaa caccctgccg ccgaccatgc tgagcgatgc
     7921 ggatctgcag caactggatg tggttctgag catcagcaac caccgtgtgc tgcgtaaggg
     7981 tggcattcgt ctggagaacc tgagctatga cagcaccgaa ctggcgaact accgtaagca
     8041 gttcagccac aaagtgagcc aagaggttct gatcaaactg aacccggacg atattagcta
     8101 catctatgtg tacctggaca agctggaaca ctatattaaa gttccgtgca tcgatccgaa
     8161 cggttacacc cagaacctga gcctgaacca acacaagatc aacattcgta tccaccgtga
     8221 ctttattagc ggtagcatcg ataacgttgg cctggcgaag gcgcgtatgt tcattcacaa
     8281 caaaatccag aacgagtttg aggaactgaa gaacgcgccg aaacacagca aggtgaaagg
     8341 tggcaaggcg ctggcgaaac accagaacat tagcagcgac agccaaaaga gcatcaccca
     8401 cagcaaaccg gtggaggcga agaaagttac cccgaaagaa caaccgaccg atagctggga
     8461 cgatttcatc agcgacctgg atggttttta attatgctga ccgacaagca gaaagaaaag
     8521 ctgaacgagt tccgtgatgt ttttattgaa tacccgatca ttaccaccat cttcaacgac
     8581 tttgatcgtc tgcgtctggg taaaggcctg accggcgaga agccgtgcat gctgctgaac
     8641 ggtgacaccg gcaccggtaa aaccgcgctg attaaacagt ataaggaacg tcacctgccg
     8701 caattcatca acggtgttat gaaccacccg gtgctggtta gccgtattcc gagcaacccg
     8761 accctggaaa gcaccctggc ggagctgctg aaagacctgg gtcaagtggg cagcaccgag
     8821 cgtaagctgc gtattaacgg cacccgtctg accaccagcc tgatcaaatg cctgaagacc
     8881 tgcggcaccg aactgatcat tatcgatgag tttcaggaac tgattgagca caaccaaggc
     8941 aagaaacgtc gtgaaattgc gaaccgtctg aaatacatca acgacgaggc gggtgttagc
     9001 attgtgctgg ttggcatgcc gtgggcggaa aagatcgcgg atgagccgca gtggagcagc
     9061 cgtctgctga tccgtcgtca actgccgtat ttcaaactga gcgagaaccc gaagcacttt
     9121 gtgcagctga ttatcggtct ggcgaaccgt atgccgttcg cggaaaaacc gaacctgagc
     9181 gagcaagcga ccgttttcac cctgtttagc ctgagcaaag gctgcttccg taccctgaag
     9241 tactttctgg acgatgcggt gctgtatgcg ctgatggaca acgcgaagac cctgaccacc
     9301 aaacacctgg tgaaggcgtt cgaagttctg tttccggatg tgccgaacct gtttaccctg
     9361 ccggttgcgg agatcaccgc gagcgaggtg gaacgttaca gcctgtataa gccggaaagc
     9421 agccaggacg aggacccgtt cattgcgacc aaatttaccg atcgtatgcc gatcagccaa
     9481 ctgctgcgta agtaactcga gccgctgagc aataactagc ataacccctt ggggcctcta
     9541 aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac
     9601 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
     9661 cctgcctccg caagtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9721 ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag
     9781 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     9841 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     9901 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     9961 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
    10021 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
    10081 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
    10141 tacgcggtcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
    10201 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
    10261 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
    10321 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
    10381 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
    10441 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
    10501 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc
    10561 ccgttgaata tggctcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10621 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggcatgct
    10681 agcgcagaaa cgtcctagaa gatgccagga ggatacttag cagagagaca ataaggccgg
    10741 agcgaagccg tttttccata ggctccgccc ccctgacgaa catcacgaaa tctgacgctc
    10801 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctgatgg
    10861 ctccctcttg cgctctcctg ttcccgtcct gcggcgtccg tgttgtggtg gaggctttac
    10921 ccaaatcacc acgtcccgtt ccgtgtagac agttcgctcc aagctgggct gtgtgcaaga
    10981 accccccgtt cagcccgact gctgcgcctt atccggtaac tatcatcttg agtccaaccc
    11041 ggaaagacac gacaaaacgc cactggcagc agccattggt aactgagaat tagtggattt
    11101 agatatcgag agtcttgaag tggtggccta acagaggcta cactgaaagg acagtatttg
    11161 gtatctgcgc tccactaaag ccagttacca ggttaagcag ttccccaact gacttaacct
    11221 tcgatcaaac cgcctcccca ggcggttttt tcgtttacag agcaggagat tacg
//
