LOCUS       sequence-403367-       14906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CRISPR-associated transposases, shuttle vector for 
            Corynebacterium glutamicum / E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Yang S, Zhu J, Zhou X, Zhang J, Li Q, Bian F, Zhu J, Yan T, Wang X, 
            Zhang Y, Yang J, Jiang Y, Yang S
  TITLE     RNA-Guided DNA Transposition in Corynebacterium glutamicum and 
            Bacillus subtilis.
  JOURNAL   ACS Synth Biol. 2023 Jul 21;12(7):2198-2202. doi: 
            10.1021/acssynbio.3c00193. Epub 2023 Jun 22.
  PUBMED    37348112
REFERENCE   2  (bases 1 to 14906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.3c00193"; journalName: "ACS Synth Biol"; date: 
            "2023-07-21- 21"; volume: "12"; issue: "7"; pages: "2198-2202"
COMMENT     Sequence Label: pCgVchCAST-crtYf
FEATURES             Location/Qualifiers
     source          1..14906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(19..46)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(138..223)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(304..927)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCKSGS"
     primer_bind     835..854
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    1347..1365
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(9916..9934)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9930..9977
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10206..11000)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10317..10336)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10927..10946
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(13959..14697)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc
       61 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
      121 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      181 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttaaattct agactgatga
      241 atcccctaat gatttttatc aaaatcatta aggttaccat cacggaaaaa ggttatgctg
      301 cttttaagac ccacttttac atttaagttg tttttctaat ccgcatatga tcaattcaag
      361 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      421 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      481 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      541 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      601 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      661 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      721 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      781 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
      841 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
      901 cactttactt ttatctaatc tagacatgtt gtgtctcctc taaagattgt aggaaatgca
      961 atgtgtcaag cagttaggtc aatcagagca tcggtcactc aagaattctt gagctgacag
     1021 aacccaaggg cgagtatggc gaatttttcc aactcacgca ccaaacctac tcacattgga
     1081 taattcgtcg agtcaactcc gtgtcaacct gtcctcaact ttagcgggga aacaaagatt
     1141 tcgcagcaga tgcaaaatca tacagacaca ttcaaaaatg gacattgacc ccaaacgggg
     1201 gcatttccaa cacttgcgct aaatagtttt tgcagggaac gaacctgaaa tcgttcgcac
     1261 tacgatctgc tcccccgaat ggggttaatt atgaactaca ttacaccccc gtgaatcgaa
     1321 ttccaaaaac taaaaaaaat attgacactc tatcattgat agagtataat taacgggatc
     1381 cactcttcat tgataagtcg ccaagcttgg gatccccagc ttgttgatac actaatgctt
     1441 ttatataggg aaaaggtggt gaactactcc atgggtgaac tgccgagtag gtagctgata
     1501 acaggcaacc atagggcagg aatcagaagt actggtgaac tgccgagtag gtagctgata
     1561 acggatccga attcgagctc ggcgcgcctg caggtcgaca agcttgcggc cgctagggaa
     1621 aaggtggtga actactatgt tcctgcagcg cccaaagcca tactccgatg agtccctcga
     1681 atccttcttc atccgcgtgg ctaacaagaa cggctacggc gacgtccacc gcttcctgga
     1741 ggcaaccaag cgcttcctcc aggatatcga ccacaacggc taccagacct tcccaaccga
     1801 tatcacccgc atcaacccat actccgccaa gaactcctcc tccgctcgca ccgcatcctt
     1861 cctgaagctc gcccagctga ccttcaacga gccaccagaa ctgctcggcc tcgctatcaa
     1921 ccgcaccaac atgaagtact ccccatccac ctccgcagtg gtccgtggtg cagaagtgtt
     1981 cccacgttcc ctgctccgca cccactccat cccatgctgc ccactgtgcc tccgcgagaa
     2041 cggctacgct tcctacctgt ggcacttcca gggctacgaa tactgccact cccacaacgt
     2101 gccactcatc accacctgct cctgcggcaa ggagttcgac taccgcgtct ccggcctgaa
     2161 gggcatctgc tgcaagtgca aggaaccaat caccctcacc tcccgcgaga acggccacga
     2221 agcagcctgc accgtgtcca actggctggc tggccacgag tccaagccac tgccaaacct
     2281 cccaaagtcc taccgttggg gtctggtgca ctggtggatg ggcatcaagg attccgaatt
     2341 cgaccacttc tccttcgtcc agttcttctc caactggcca cgctccttcc actccatcat
     2401 cgaagatgag gtcgaattca acctggagca cgcagtggtc tccacctccg aactgcgtct
     2461 caaggacctg ctcggtcgtc tgttcttcgg ctccatccgc ctgccagaac gcaacctcca
     2521 gcacaacatc atcctcggcg agctgctctg ctacctggaa aaccgcctct ggcaggataa
     2581 gggcctgatc gcaaacctca agatgaacgc cctggaggct accgtgatgc tgaactgctc
     2641 cctcgaccag atcgcctcca tggtcgaaca gcgcatcctg aagccaaacc gcaagtccaa
     2701 gccaaactcc ccactggatg tgaccgacta cctcttccac ttcggcgata tcttctgcct
     2761 gtggctcgca gagttccagt ccgacgaatt caaccgctcc ttctacgtct cgaggtggta
     2821 aatgcagacc ctgaaggagc tcatcgcctc caacccagat gacctgacca ccgaactcaa
     2881 gcgcgctttc cgcccactga ccccacacat cgcaatcgat ggcaacgagc tggacgccct
     2941 caccatcctg gtgaacctca ccgataagac cgatgaccag aaggatctgc tcgaccgcgc
     3001 aaagtgcaag cagaagctgc gcgacgaaaa gtggtgggcc tcctgcatca actgcgtgaa
     3061 ctaccgccag tcccacaacc caaagttccc agatatccgt tccgagggtg tcatccgtac
     3121 ccaggcactg ggcgaactcc catccttcct gctctcctcc tccaagatcc caccatacca
     3181 ctggtcctac tcccacgatt ccaagtacgt caacaagtcc gccttcctga ccaacgaatt
     3241 ctgctgggac ggcgagatct cctgcctggg tgaactgctc aaggatgctg accacccact
     3301 ctggaacacc ctgaagaagc tcggctgctc ccagaagacc tgcaaggcta tggcaaagca
     3361 gctcgctgat atcaccctga ccaccatcaa cgtgaccctc gcaccaaact acctgaccca
     3421 gatctccctc ccagattccg acacctccta tatctccctg tccccagtcg cttccctctc
     3481 catgcagtcc cacttccacc agcgcctgca ggatgaaaac cgccactccg caatcacccg
     3541 cttctcccgc accaccaaca tgggtgtgac cgctatgacc tgcggtggtg cattccgcat
     3601 gctgaagtcc ggcgcaaagt tctcctcccc accacaccac cgcctgaact ccaagcgctc
     3661 ctggctcacc tccgaacacg tgcagtccct gaagcagtac cagcgcctga acaagtccct
     3721 catcccagag aactcccgca tcgcactgcg ccgcaagtac aagatcgaac tccagaacat
     3781 ggtccgctcc tggttcgcca tgcaggatca caccctggac tccaacatcc tgatccagca
     3841 cctcaaccac gatctgtcct acctcggcgc caccaagcgc ttcgcatacg acccagccat
     3901 gaccaagctg ttcaccgaac tgctcaagcg cgagctctcc aactccatca acaacggcga
     3961 gcagcacacc aacggctcct tcctggtgct cccaaacatc cgtgtctgcg gtgcaaccgc
     4021 tctgtcctcc ccagtgaccg tcggcatccc atccctcacc gccttcttcg gcttcgtgca
     4081 cgctttcgaa cgcaacatca accgcaccac ctcctccttc cgcgtcgagt ccttcgctat
     4141 ctgcgtgcac cagctgcacg tcgaaaagcg cggcctcacc gcagaattcg tggagaaggg
     4201 cgatggcacc atctccgcac cagccacccg cgatgactgg cagtgcgacg tggtcttctc
     4261 cctgatcctc aacaccaact tcgcccagca catcgatcag gacaccctgg tcacctccct
     4321 cccaaagcgt ctggctcgtg gttccgcaaa gatcgctatc gatgacttca agcacatcaa
     4381 ctccttctcc accctggaaa ccgctatcga gtccctccca atcgaggcag gtcgttggct
     4441 gtccctctac gcacagtcca acaacaacct gtccgatctg ctcgcagcca tgaccgaaga
     4501 ccaccagctc atggcatcct gcgtgggcta ccacctgctc gaagagccaa aggataagcc
     4561 aaactccctg cgcggctaca agcacgctat cgcagagtgc atcatcggcc tcatcaactc
     4621 catcaccttc tcctccgaaa ccgacccaaa caccatcttc tggtccctga agaactacca
     4681 gaactacctc gtggtccagc caaggagtat caacgatgaa actaccgaca aatctagcct
     4741 atgagcgctc catcgatcca tccgacgtct gcttcttcgt ggtctggcca gatgaccgca
     4801 agaccccact cacctacaac tcccgcaccc tgctcggcca gatggaagca gcctccctgg
     4861 cttacgatgt gtccggccag ccaatcaagt ccgcaaccgc tgaggcactc gcacagggta
     4921 acccacacca ggtggacttc tgccacgtcc catacggcgc atcccacatc gaatgctcct
     4981 tctccgtctc cttctcctcc gagctgcgcc agccatacaa gtgcaactcc tccaaggtga
     5041 agcagaccct ggtgcagctc gtcgaactgt acgagaccaa gatcggctgg accgaactcg
     5101 ccacccgcta cctgatgaac atctgcaacg gcaagtggct ctggaagaac acccgcaagg
     5161 cttactgctg gaacatcgtc ctgaccccat ggccatggaa cggcgaaaaa gtgggcttcg
     5221 aggatatccg caccaactac acctcccgcc aggacttcaa gaacaacaag aactggtccg
     5281 caatcgtcga aatgatcaag accgccttct cctccaccga tggcctggcc atcttcgagg
     5341 tgcgcgctac cctgcacctc ccaaccaacg ccatggtgcg cccatcccag gtcttcaccg
     5401 aaaaggagtc cggctccaag tccaagtcca agacccagaa ctcccgcgtc ttccagtcca
     5461 ccaccatcga cggtgaacgt tccccaatcc tcggtgcttt caagaccggc gctgcaatcg
     5521 caaccatcga tgactggtac ccagaagcaa ccgagccact gcgtgtgggt cgtttcggtg
     5581 tccaccgcga agatgtgacc tgctaccgcc acccatccac cggcaaggac ttcttctcca
     5641 tcctccagca ggcagaacac tacatcgagg tgctgtccgc taacaagacc ccagcacagg
     5701 agaccatcaa cgatatgcac ttcctcatgg ccaacctgat caagggcggc atgttccagc
     5761 acaaaggaga ctgactgtga agtggtacta caagaccatc accttcctcc cagaactgtg
     5821 caacaacgag tccctcgcag caaagtgcct ccgtgtgctg cacggcttca actaccagta
     5881 cgaaacccgc aacatcggcg tctccttccc actctggtgc gatgctaccg tgggcaagaa
     5941 gatctccttc gtctccaaga acaagatcga actggacctg ctcctgaagc agcactactt
     6001 cgtgcagatg gagcagctcc agtacttcca catctccaac accgtgctgg tcccagagga
     6061 ttgcacctac gtctccttcc gccgctgcca gtccatcgac aagctcaccg ctgcaggtct
     6121 ggctcgtaag atccgtcgtc tcgaaaagcg tgcactgtcc cgtggcgagc agttcgatcc
     6181 atcctccttc gcacagaagg aacacaccgc catcgctcac taccactccc tgggcgagtc
     6241 ctccaagcag accaaccgca acttccgcct caacatccgc atgctgtccg aacagccacg
     6301 cgagggcaac tccatcttct cctcctacgg cctctccaac tccgaaaact ccttccagcc
     6361 agtgccactg atctaatagg gaaaaggtgg tgaactacta tggcaacctc cctgccaacc
     6421 ccatccgcaa tcaccacctc cgccctggaa tacgcattcc acaccccagc acgcaacctg
     6481 accaagtccc gcggcaagaa catccaccgc tacgtgtccg tgaagatgtc caagcgcatc
     6541 accgtggaat ccaccctgga atgcgatgca tgctaccact tcgacttcga accatccatc
     6601 gtgcgcttct gcgcacagcc aatccgcttc ctgtactacc tgaacggcca gtcccactcc
     6661 tacgtgccag acttcctggt gcagttcgac accaacgaat ttgtgctgta cgaagtgaag
     6721 tccgcatacg caaagaacaa gccagacttc gatgtggaat gggaagcaaa ggtgaaggcc
     6781 gcaaccgaac tgggcctgga actggaactg gtggaagaat ccgacatccg cgacaccgtg
     6841 gtgctgaaca acctgaagcg catgcaccgc tacgcctcca aggacgaact gaacaacgtg
     6901 cacaactccc tgctgaagat catcaagtac aacggcgcac agtccgcacg ctgcctgggc
     6961 gaacagctgg gcctgaaggg ccgcaccgtg ctgccaatcc tgtgcgacct gctgtcccgc
     7021 tgcctgctgg atacccgcct ggataagcca ctgtccctgg aatcccgctt cgagttggcc
     7081 agttatggct aagaagggct tctcctcctt ccaccgcaag gctgtgtcct cccaggatac
     7141 cctcgaatcc atcgagctgg tgtcctccgc aaactgcctc gaatccgtca cctaccagga
     7201 catctccgct ttcccagaga ccatcgcagt cgaaatcaac ttccgcctgt ccatcctccg
     7261 cttcctggca cgcaagtgcg aaaccatcgt ggccaagtcc atcgagccac accgcgtcga
     7321 actgcagcag aactactccc gcaagatccc atccgccatc accatctacc gctggtggct
     7381 cgctttccgc aagtccgatt acaacccaat ctccctggcc ccaaacatca aggaccgcgg
     7441 caaccgcgaa accaaggtgt ccaccgtggt cgattccatc atggagcagg cagtggaacg
     7501 cgtcatctcc ggccgcaagg tgaacgtctc ctccgcatac aagcgtgtgc gtcgtaaggt
     7561 ccgccagtac aacctgaccc acggcaccaa gtacacctac ccaaagtacg agtccgtgcg
     7621 caagcgcgtg aagaagaaga ccccattcga actgctcgca gcaggcaagg gcgagcgtgt
     7681 ggctaagcgc gaattccgcc gcatgggcaa gaagatcctc acctcctccg tgctggaacg
     7741 cgtcgagatc gatcacaccg tggtcgacct cttcgcagtc cacgaagagt accgtatccc
     7801 actgggtcgt ccatggctca cccagctggt ggattgctac tccaaggccg tcatcggctt
     7861 ctacctgggc ttcgagccac catcctacgt gtccgtctcc ctggcactca agaacgccat
     7921 ccagcgcaag gatgacctca tctcctccta cgaatccatc gaaaacgagt ggctgtgcta
     7981 cggcatccca gatctgctcg tgaccgacaa cggcaaggag ttcctgtcca aggctttcga
     8041 tcaggcatgc gaatccctgc tcatcaacgt gcaccagaac aaggtcgaga ccccagacaa
     8101 caagccacac gtggaacgca actacggcac catcaacacc tccctgctcg atgacctccc
     8161 tggcaagtcc ttctcccagt acctgcagcg cgagggctac gattccgtcg gcgaagcaac
     8221 cctgaccctc aacgaaatcc gcgagatcta cctcatctgg ctggtggaca tctaccacaa
     8281 gaagccaaac cagcgcggca ccaactgccc aaacgtcgct tggaagaagg gctgccagga
     8341 atgggagcca gaagagttct ccggctccaa ggatgaactc gacttcaagt tcgcaatcgt
     8401 ggattacaag cagctgacca aagtgggcat caccgtctac aaggagctct cctactccaa
     8461 cgaccgcctg gccgaatacc gcggcaagaa gggcaaccac aaggtgcagt tcaagtacaa
     8521 cccagagtgc atggctgtga tctgggtcct ggatgaggac atgaacgaat acttcaccgt
     8581 gaacgccatc gattacgaat acgcttcccg cgtctccctc tggcagcaca agtacaacat
     8641 gaagtaccag gccgagctga actccgctga atacgatgag gacaaggaaa tcgatgctga
     8701 aatcaagatc gaagagatcg cagaccgctc catcgtgaag accaacaaga tccgtgcacg
     8761 tcgtcgtggt gcacgtcacc aggaaaactc cgctcgcgca aagtccatct ccaacgccaa
     8821 cccagcttcc atccagaagc acgaagatga gatcgtgtcc gccgacaacg atgactggga
     8881 tatcgactac gtctgagaaa tcgtcaaatg tccgaaaccc gtgaggctcg tatctcccgt
     8941 gctaagcgcg cattcgtgtc caccccatcc gtccgcaaga tcctctccta catggatcgc
     9001 tgccgcgatc tgtccgacct cgaatccgag ccaacctgca tgatggtgta cggtgcatcc
     9061 ggtgtgggca agaccaccgt catcaagaag tacctgaacc agaaccgtcg tgaatccgag
     9121 gcaggtggtg acatcatccc agtcctgcac atcgaactcc cagataacgc taagccagtg
     9181 gacgcagccc gcgaactgct cgtcgagatg ggcgatccac tggctctcta cgaaaccgac
     9241 ctggcacgcc tcaccaagcg tctgaccgag ctcatcccag cagtgggcgt caagctgatc
     9301 atcatcgatg aattccagca cctcgtggaa gagcgttcca accgtgtgct gacccaggtc
     9361 ggcaactggc tgaagatgat cctcaacaag accaagtgcc caatcgtcat cttcggcatg
     9421 ccatactcca aggtggtcct gcaggctaac tcccagctcc acggccgctt ctccatccag
     9481 gtggaactgc gtccattctc ctaccagggt ggtcgtggcg tcttcaagac cttcctggag
     9541 tacctcgaca aggcactgcc attcgaaaag caggccggcc tcgctaacga gtccctgcag
     9601 aagaagctct acgccttctc ccagggcaac atgcgctccc tgcgcaacct catctaccag
     9661 gcatccatcg aagccatcga taaccagcac gagaccatca ccgaagagga cttcgtgttc
     9721 gcatccaagc tgacctccgg cgataagcca aactcctgga agaacccatt cgaagagggc
     9781 gtggaagtca ccgaggacat gctccgccca ccaccaaagg atatcggctg ggaagactac
     9841 ctgcgtcact ccaccccacg tgtgtccaag ccaggtcgca acaagaactt cttcgagtaa
     9901 cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     9961 tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc acactgcttc
    10021 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
    10081 ctgaaccgac gacaagctga cgaccggtgg cacttttcgg ggaaatgtgc gcggaacccc
    10141 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcaagct
    10201 tcatatcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
    10261 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
    10321 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
    10381 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
    10441 gtcacgacga gatcctcgcc gtcgggcatc cgcgccttga gcctggcgaa cagttcggct
    10501 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
    10561 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
    10621 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
    10681 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
    10741 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
    10801 agccgcgctg cctcgtcttg gagttcattc agggcaccgg acaggtcggt cttgacaaaa
    10861 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
    10921 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
    10981 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc agatcttgat
    11041 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
    11101 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
    11161 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
    11221 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
    11281 tgcggactgg ctttctacgt gttccgcttc cggtgaccgc ctcgatgatc gccgggtggg
    11341 cgtggcccaa ggaggatcat ccagccattc ggggtcgttc actggttccc ctttctgatt
    11401 tctggcatag aagaaccccc gtgaactgtg tggttccggg ggttgctgat ttttgcgaga
    11461 cttctcgcgc aattccctag cttaggtgaa aacaccatga aacactaggg aaacacccat
    11521 gaaacaccca ttagggcagt agggcggctt cttcgtctag ggcttgcatt tgggcggtga
    11581 tctggtcttt agcgtgtgaa agtgtgtcgt aggtggcgtg ctcaatgcac tcgaacgtca
    11641 cgtcatttac cgggtcacgg tgggcaaaga gaactagtgg gttagacatt gttttcctcg
    11701 ttgtcggtgg tggtgagctt ttctagccgc tcggtaaacg cggcgatcat gaactcttgg
    11761 aggttttcac cgttctgcat gcctgcgcgc ttcatgtcct cacgtagtgc caaaggaacg
    11821 cgtgcggtga ccacgacggg cttagccttt gcctgcgctt ctagtgcttc gatggtggct
    11881 tgtgcctgcg cttgctgcgc ctgtagtgcc tgttgagctt cttgtagttg ctgttctagc
    11941 tgtgccttgg ttgccatgct ttaagactct agtagctttc ctgcgatatg tcatgcgcat
    12001 gcgtagcaaa cattgtcctg caactcattc attatgtgca gtgctcctgt tactagtcgt
    12061 acatactcat atttacctag tctgcatgca gtgcatgcac atgcagtcat gtcgtgctaa
    12121 tgtgtaaaac atgtacatgc agattgctgg gggtgcaggg ggcggagcca ccctgtccat
    12181 gcggggtgtg gggcttgccc cgccggtaca gacagtgagc accggggcac ctagtcgcgg
    12241 ataccccccc taggtatcgg acacgtaacc ctcccatgtc gatgcaaatc tttaacattg
    12301 agtacgggta agctggcacg catagccaag ctaggcggcc accaaacacc actaaaaatt
    12361 aatagtccct agacaagaca aacccccgtg cgagctacca actcatatgc acgggggcca
    12421 cataacccga aggggtttca attgacaacc atagcactag ctaagacaac gggcacaaca
    12481 cccgcacaaa ctcgcactgc gcaaccccgc acaacatcgg gtctaggtaa cactgaaata
    12541 gaagtgaaca cctctaagga accgcaggtc aatgagggtt ctaaggtcac tcgcgctagg
    12601 gcgtggcgta ggcaaaacgt catgtacaag atcaccaata gtaaggctct ggcggggtgc
    12661 cataggtggc gcagggacga agctgttgcg gtgtcctggt cgtctaacgg tgcttcgcag
    12721 tttgagggtc tgcaaaactc tcactctcgc tgggggtcat ctctggctga attggaagtc
    12781 atgggcgaac gccgcattga gctggctatt gctactaaga atcacttggc ggcgggtggc
    12841 gcgctcatga tgtttgtggg cactgttcga cacaaccgct cacagtcatt tgcgcaggtt
    12901 gaagcgggta ttaagactgc gtactcttcg atggtgaaaa catctcagtg gaagaaagaa
    12961 cgtgcacggt acggggtgga gcacacctat agtgactatg aggtcacaga ctcttgggcg
    13021 aacggttggc acttgcaccg caacatgctg ttgttcttgg atcgtccact gtctgacgat
    13081 gaactcaagg cgtttgagga ttccatgttt tcccgctggt ctgctggtgt ggttaaggcc
    13141 ggtatggacg cgccactgcg tgagcacggg gtcaaacttg atcaggtgtc tacctggggt
    13201 ggagacgctg cgaaaatggc aacctacctc gctaagggca tgtctcagga actgactggc
    13261 tccgctacta aaaccgcgtc taaggggtcg tacacgccgt ttcagatgtt ggatatgttg
    13321 gccgatcaaa gcgacgccgg cgaggatatg gacgctgttt tggtggctcg gtggcgtgag
    13381 tatgaggttg gttctaaaaa cctgcgttcg tcctggtcac gtggggctaa gcgtgctttg
    13441 ggcattgatt acatagacgc tgatgtacgt cgtgaaatgg aagaagaact gtacaagctc
    13501 gccggtctgg aagcaccgga acgggtcgaa tcaacccgcg ttgctgttgc tttggtgaag
    13561 cccgatgatt ggaaactgat tcagtctgat ttcgcggtta ggcagtacgt tctcgattgc
    13621 gtggataagg ctaaggacgt ggccgctgcg caacgtgtcg ctaatgaggt gctggcaagt
    13681 ctgggtgtgg attccacccc gtgcatgatc gttatggatg atgtggactt ggacgcggtt
    13741 ctgcctactc atggggacgc tactaagcgt gatctgaatg cggcggtgtt cgcgggtaat
    13801 gagcagacta ttcttcgcac ccactaaaag cggcataaac cccgttcgat attttgtgcg
    13861 atgaatttat ggtcaatgtc gcgggggcaa actatgatgg gtcttgttgt ttagctagct
    13921 cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt
    13981 tacccacaga ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc
    14041 gccggtggcg tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt
    14101 ttccggtgca tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac
    14161 ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg
    14221 ttccgaccct gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc
    14281 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
    14341 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
    14401 cttgagtcca acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg
    14461 gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt
    14521 ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt
    14581 taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg
    14641 tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    14701 tctactgaac cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
    14761 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
    14821 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
    14881 gctaacttac attaattgcg ttgcgc
//
