LOCUS       sequence-403379-        8412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for Igf1 enhancer 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, 
            Spiegel I
  TITLE     Single genomic enhancers drive experience-dependent GABAergic 
            plasticity to maintain sensory processing in the adult cortex.
  JOURNAL   Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026.
  PUBMED    37354902
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026."
COMMENT     Sequence Label: pGL4.11_Nued2_E3
FEATURES             Location/Qualifiers
     source          1..8412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             406..525
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    577..698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(588..607)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     642..661
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(946..963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1117..1705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1197..1216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1905..2696,2697..2765))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2870..2918
                     /note="synthetic polyadenylation signal"
     misc_feature    2932..3023
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2972..2991
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      7159..7168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7165..8412,1..402)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac
       61 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc
      121 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat
      181 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag
      241 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt
      301 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc
      361 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgaattctca cggcttccct
      421 cccgaggtgg aggagcaggc cgccggcacc ctgcccatga gctgcgccca ggagagcggc
      481 atggatagac accctgctgc ttgcgccagc gccaggatca acgtctaagg ccgcgactct
      541 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac
      601 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      661 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      721 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      781 aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc
      841 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
      901 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
      961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1741 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1801 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1861 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
     1921 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
     1981 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
     2041 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
     2101 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
     2161 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
     2221 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
     2281 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
     2341 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
     2401 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
     2461 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
     2521 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
     2581 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
     2641 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
     2701 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
     2761 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
     2821 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
     2881 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     2941 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     3001 tgcaggtgcc agaacatttc tctggcctaa ctggccggta cctgagctcg ctagcttcct
     3061 ggcttgtagt gacctcgtct attgctagaa atcagaaggt accactgagc cttcatttgt
     3121 ttggtgaaat catggagtta cattaacaag ccgtttcccc aaactatggc tctctaccca
     3181 cttgtaatac acccgtgcac aggaacattt tctgaaagtc aatgtttcct atcacttgct
     3241 ccaaaattaa accagagata catcattcag gaagaagcca gatgcactga agaaagaaaa
     3301 acctgcttta tcagaagggg caagcttctc tcacatccca tagcaattta aattcatttg
     3361 ccggaatgtt aaatacaacc agtcttttag ttgggtccct tcacataaac accaagttag
     3421 cataaatgca aaatagttca catgattaaa tctttataac accataattt atcttatgta
     3481 cacacacaca cacagagaaa gagagagaga gagagagaga gagagatctt ttacttgctg
     3541 atacttctac attttttctg gagttctcaa ctctcatcat ctctctaagg aagctggctt
     3601 gtattgcccc agactcttag tgtatttagt tctaaaggat agtatacaga ataaagggaa
     3661 attttaattt tgtttttaat aggacctata taattcaggc ctagctctgg tggagagaag
     3721 gggaggagag gtccattagc atttattgtg cacctgcttt atgctaaaca tgtctggaaa
     3781 cagtgtctca caccataggc agtgagtgtc tagattttag aagtctagaa ctgagccctg
     3841 acttcatata attcatgtgc cctggcctta atcttggcac tttagtagtg ctaaaggtcc
     3901 ccaggccctc ccttagggtc agaacatggt taataagaga cacagggcat gtgattacag
     3961 actagcaaga gtgttacact cacaggtgcc tcagcccatg agtcatggct tgtagccttg
     4021 cattccaaga agttctggat ccctgcagct cagccaattt tggacattaa tgtccccaga
     4081 acaacaagta gatgctgtac tgtggccaag gcaacagcag ctgcttctga tgctaccatg
     4141 aggtaaagaa gggacacaaa actaaattca cctcatataa caaacaaacc agaagaaaaa
     4201 tcaattaaac ctccaatact ttttttattt ttataaccta aaaaactata tatacacctg
     4261 gtaaatatgc aatgtaatac agacaattgt gttttaattt aaattatttc tcacaagagg
     4321 cttttttaaa aagtatattc actaaaatat ttggatcatt gtgaaaactt catttactgc
     4381 ttgaaatatc taaaattctc tacctcgctt ggttgtatgg acacagttct gaattgcatt
     4441 tacctcaaag aaaaatacca gatatgctga tttgaaggaa aagaaaaaaa gcttggcatt
     4501 tgagagaaat aatgaacctc ctcaaagcat tccttttttt ttttttaaag agcttcagaa
     4561 aaatcaacaa gaaagaaagg ctgatatgca gtgcttttaa gcattctgtc agatttgacc
     4621 atgtacagat ccccacatat ccacagagtt gaaacatgtt ctctcaaatt taaagcagac
     4681 tacctgagtg atcaaagcgc gtttaaacgt gtggcgcgcc agctagtaac agttggcatt
     4741 gggactagaa ggcctcattc ctaaaccagc agatttttcc attgtaccag gaaaacaaaa
     4801 ctgcaaatag cataagtcct ctgattttac tgtctccaga gaaaatcata tcctcaatgc
     4861 ctgggtcttt ccatctggaa tttggcaact ctgctgatcc cttctcatag tgggaactct
     4921 tgtaggaagt ctaagagcaa ttattttggg gatatatgct atttttatgt cttcttgtac
     4981 atcaccttct ttggtcagga ggaaattaga attctggaaa tacgtttaat tattaaaagg
     5041 acaagccttg gcttcaagta gttgcaaata tgaattatta gtccatgtca tgaaagggct
     5101 ggagaactac aaagaaacaa tgataatcta tttgtcacac ccaattaaga tctgccctgg
     5161 cctgggagta aaagagcatt aaatctgtga gtggtaactt caagcagcat gtctatattt
     5221 gggtaattct gaacatgcct ggatttggta ccttacagca tcctctaaaa atgtactact
     5281 ttagaaagtg ttttgagatt ttgttgcctt cgtgattgca gtttgtgtgt gtgtgtgtgt
     5341 gtggggggtg ttatttgagc ttttgagttt ccatgtagcc aaagaaagaa agtctaggaa
     5401 gaaatggtag actcaaggtc aaattcaaca aggggaaact tctttgtggt gagtctagac
     5461 aacaaagcca ttagcccagt gttcaccccc ctgcctacac cctacctttt ccattgatga
     5521 atattcttac ctgagccatc ctcgcttctg agtcctgctc tgggtcctgt ggagactgaa
     5581 tatcctgtct gtagttttta atccttgaag agatttttca tgaatagaac tttccttata
     5641 atttagcaac actgtccttg aaaaccccca caactattta cataacagaa gctattgttt
     5701 gaagtgcaaa acaggtcaga atttggttcc cagagattaa tcactgcagt ctattttgta
     5761 ttatcataat aatttcattt attttgatac aatttgtttt cagaatcaac cccagcttct
     5821 ttgcacaagt gacaaatcca tttgtttctg gtagaatggg taatgtgatt aatgaccttg
     5881 caacaggatt tctttaaaaa gggggaactt tttttttttc atttttaatt cacagtaaac
     5941 cagctgagtc tgttaagtgt gtaactgttc tgaacttgta agccatgggt aagattttga
     6001 aataccccct cccaatcttt tgcggggttt ctgggtatta atattaggct ttctccaaga
     6061 atctgatgat actagtctcc aggtgtaaag tggacttgtt ggattcccgc attaaaaaga
     6121 acggggcccc agtctttgcc atggccccat catgtttatt ccggctggat gttctcatca
     6181 aaccgaggtc atgcgaaata aataaggtca tgcctagaag gatattgtcc ccgcacaaga
     6241 accttcgagt gaactcgtga ctactgcctc ctggtcttct gtgtgggcat ccccttcttt
     6301 cttgcagcat ccaggaccca cttctgtccc tgctggtctg tttctttctt tctttctttc
     6361 tttctttctt tctttcttgt tttcttccgt ttttcccctt gagttctctg gggttgggcc
     6421 tgggattttg ggtacgccta cagactgtcc gcctctgcaa tcgaccgtcg tgcttcccgc
     6481 gccagaacaa ggaagcccac gcgagccgcg taggtggctc gcgtggaaga cccgggttcg
     6541 agtcccgctg ggagaattcc tcctaggcaa gtggagtcat ctcggtcagg atttctcagc
     6601 cggaagccgc gccaacacct ctcatcgtgg gcggcaagtt gtccccgggg cttctttgac
     6661 cgtgcggggc cagccgacgc tttctctgac cgtttccgaa gcccagtttc ctttcctaag
     6721 gagccttggg gatcctgcac tcgcgtctct gaaacccaac ccacgacaga acccagccgc
     6781 ctgggccagc ttagcaatgc gcccgcgccc cgccctccgc gtgacgcgaa cgccggcgga
     6841 ccaatcagag agcgcggcgc gagccccacg cgcctgtggc cccaccctcc aagcttaaga
     6901 aagggcgcgc gggcagagcc cgggccagaa cgccgcgcaa agcggagagc gggtttttgc
     6961 gacggcaggc ggcgcttgtg ctgccgtttc cgtgctgcac gcggttccct cgagtgccgc
     7021 gctcggacag acgtagccgg ccgcgaaggc gcccagacgg caaaccagcg acccatgctg
     7081 aagtgagcac acaggtcagc gaggatatca agatctggcc tcggcggcca agcttggcaa
     7141 tccggtactg ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg
     7201 ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc
     7261 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc
     7321 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg
     7381 aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg
     7441 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc
     7501 gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg
     7561 ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg
     7621 gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg
     7681 ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc
     7741 gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac
     7801 cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc
     7861 cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg
     7921 ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta
     7981 ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt
     8041 agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc
     8101 gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac
     8161 ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc
     8221 cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag
     8281 gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc
     8341 cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc
     8401 gacaaggacg gc
//
