LOCUS       sequence-403382-        8411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for Igf1 enhancer 5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, 
            Spiegel I
  TITLE     Single genomic enhancers drive experience-dependent GABAergic 
            plasticity to maintain sensory processing in the adult cortex.
  JOURNAL   Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026.
  PUBMED    37354902
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026."
COMMENT     Sequence Label: pGL4.11_Nued2_E5
FEATURES             Location/Qualifiers
     source          1..8411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             371..490
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    542..663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(553..572)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     607..626
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(911..928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1082..1670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1162..1181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1870..2661,2662..2730))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2835..2883
                     /note="synthetic polyadenylation signal"
     misc_feature    2897..2988
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2937..2956
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      7123..7132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7129..8411,1..367)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca agggctacca
       61 ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct tcgacgccgg
      121 ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga
      181 acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc aggttacaac
      241 cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg
      301 caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg gcggcaagat
      361 cgccgtgaat tctcacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc
      421 catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag
      481 gatcaacgtc taaggccgcg actctagagt cggggcggcc ggccgcttcg agcagacatg
      541 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      601 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      661 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      721 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat
      781 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      841 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1501 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1801 aatcaatcta aagtatatat gagtaaactt ggtctgacag cggccgcaaa tgctaaacca
     1861 ctgcagtggt taccagtgct tgatcagtga ggcaccgatc tcagcgatct gcctatttcg
     1921 ttcgtccata gtggcctgac tccccgtcgt gtagatcact acgattcgtg agggcttacc
     1981 atcaggcccc agcgcagcaa tgatgccgcg agagccgcgt tcaccggccc ccgatttgtc
     2041 agcaatgaac cagccagcag ggagggccga gcgaagaagt ggtcctgcta ctttgtccgc
     2101 ctccatccag tctatgagct gctgtcgtga tgctagagta agaagttcgc cagtgagtag
     2161 tttccgaaga gttgtggcca ttgctactgg catcgtggta tcacgctcgt cgttcggtat
     2221 ggcttcgttc aactctggtt cccagcggtc aagccgggtc acatgatcac ccatattatg
     2281 aagaaatgca gtcagctcct tagggcctcc gatcgttgtc agaagtaagt tggccgcggt
     2341 gttgtcgctc atggtaatgg cagcactaca caattctctt accgtcatgc catccgtaag
     2401 atgcttttcc gtgaccggcg agtactcaac caagtcgttt tgtgagtagt gtatacggcg
     2461 accaagctgc tcttgcccgg cgtctatacg ggacaacacc gcgccacata gcagtacttt
     2521 gaaagtgctc atcatcggga atcgttcttc ggggcggaaa gactcaagga tcttgccgct
     2581 attgagatcc agttcgatat agcccactct tgcacccagt tgatcttcag catcttttac
     2641 tttcaccagc gtttcggggt gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat
     2701 gagtgcgaca cgaaaatgtt ggatgctcat actcgtcctt tttcaatatt attgaagcat
     2761 ttatcagggt tactagtacg tctctcaagg ataagtaagt aatattaagg tacgggaggt
     2821 attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt
     2881 gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag
     2941 caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctgg cctaactggc
     3001 cggtacctga gctcgctagc ttcctggctt gtagtgacct cgtctattgc tagaaatcag
     3061 aaggtaccac tgagccttca tttgtttggt gaaatcatgg agttacatta acaagccgtt
     3121 tccccaaact atggctctct acccacttgt aatacacccg tgcacaggaa cattttctga
     3181 aagtcaatgt ttcctatcac ttgctccaaa attaaaccag agatacatca ttcaggaaga
     3241 agccagatgc actgaagaaa gaaaaacctg ctttatcaga aggggcaagc ttctctcaca
     3301 tcccatagca atttaaattc atttgccgga atgttaaata caaccagtct tttagttggg
     3361 tcccttcaca taaacaccaa gttagcataa atgcaaaata gttcacatga ttaaatcttt
     3421 ataacaccat aatttatctt atgtacacac acacacacag agaaagagag agagagagag
     3481 agagagagag atcttttact tgctgatact tctacatttt ttctggagtt ctcaactctc
     3541 atcatctctc taaggaagct ggcttgtatt gccccagact cttagtgtat ttagttctaa
     3601 aggatagtat acagaataaa gggaaatttt aattttgttt ttaataggac ctatataatt
     3661 caggcctagc tctggtggag agaaggggag gagaggtcca ttagcattta ttgtgcacct
     3721 gctttatgct aaacatgtct ggaaacagtg tctcacactc cctcctctcc atcactcatg
     3781 tgagcagctc ttaggccagt caagttgaaa cataagattg accatcacac cagttgaatc
     3841 aacaaatgac attcacaata ctgtcttcta caggtcacag cagatatcac ttatgtaaac
     3901 acaaccattg gtttagatga atgtatccac aggggcaagg ttactagtag tgagcctata
     3961 cttaactatt agctgttcta aatagtgtga gtcacaccat ctctagaaat agaccaaacg
     4021 aatctttgaa cagagaaaag atgatctaaa aagtttatat atattatcct ggccagcctt
     4081 gtaatagggc aaaaatggac tgatccttgc accgaccagc tgtccttctt tactctttcc
     4141 ttttaacaaa tgcaaagctc attctaaaag aagcattata ccaattcaaa gctttttttt
     4201 tatcaaatcc ccataattat tttaactatg tagcatagtc tgcacacagc cagtgattaa
     4261 gccagaccac aattaaaaca atcccgtgaa gctaatgaca aatgagctag acagactgct
     4321 accccttgct tggtgtctga atatggttct attcttcaga catcattaaa agcttccaac
     4381 atatttttaa atccctggct gtctgaggat cggtggggtc agggaagaat gagcggggac
     4441 aaagaatggg aaggaatcct gagtgaaatt tgggctgttc ggggtaagct tttcatcatg
     4501 aaagaagaaa gtaactaaaa atgcttcttc ggaaattttt tcccctcttt acaaaaactg
     4561 ttgaatagga aagtcagatt gtttgactga agctttttag aaagtagaaa gccgtagatg
     4621 atgtttcccc catttagaca aagttcctgt acctccctaa gcgcgtttaa acgtgtggcg
     4681 cgccagctag taacagttgg cattgggact agaaggcctc attcctaaac cagcagattt
     4741 ttccattgta ccaggaaaac aaaactgcaa atagcataag tcctctgatt ttactgtctc
     4801 cagagaaaat catatcctca atgcctgggt ctttccatct ggaatttggc aactctgctg
     4861 atcccttctc atagtgggaa ctcttgtagg aagtctaaga gcaattattt tggggatata
     4921 tgctattttt atgtcttctt gtacatcacc ttctttggtc aggaggaaat tagaattctg
     4981 gaaatacgtt taattattaa aaggacaagc cttggcttca agtagttgca aatatgaatt
     5041 attagtccat gtcatgaaag ggctggagaa ctacaaagaa acaatgataa tctatttgtc
     5101 acacccaatt aagatctgcc ctggcctggg agtaaaagag cattaaatct gtgagtggta
     5161 acttcaagca gcatgtctat atttgggtaa ttctgaacat gcctggattt ggtaccttac
     5221 agcatcctct aaaaatgtac tactttagaa agtgttttga gattttgttg ccttcgtgat
     5281 tgcagtttgt gtgtgtgtgt gtgtgtgggg ggtgttattt gagcttttga gtttccatgt
     5341 agccaaagaa agaaagtcta ggaagaaatg gtagactcaa ggtcaaattc aacaagggga
     5401 aacttctttg tggtgagtct agacaacaaa gccattagcc cagtgttcac ccccctgcct
     5461 acaccctacc ttttccattg atgaatattc ttacctgagc catcctcgct tctgagtcct
     5521 gctctgggtc ctgtggagac tgaatatcct gtctgtagtt tttaatcctt gaagagattt
     5581 ttcatgaata gaactttcct tataatttag caacactgtc cttgaaaacc cccacaacta
     5641 tttacataac agaagctatt gtttgaagtg caaaacaggt cagaatttgg ttcccagaga
     5701 ttaatcactg cagtctattt tgtattatca taataatttc atttattttg atacaatttg
     5761 ttttcagaat caaccccagc ttctttgcac aagtgacaaa tccatttgtt tctggtagaa
     5821 tgggtaatgt gattaatgac cttgcaacag gatttcttta aaaaggggga actttttttt
     5881 tttcattttt aattcacagt aaaccagctg agtctgttaa gtgtgtaact gttctgaact
     5941 tgtaagccat gggtaagatt ttgaaatacc ccctcccaat cttttgcggg gtttctgggt
     6001 attaatatta ggctttctcc aagaatctga tgatactagt ctccaggtgt aaagtggact
     6061 tgttggattc ccgcattaaa aagaacgggg ccccagtctt tgccatggcc ccatcatgtt
     6121 tattccggct ggatgttctc atcaaaccga ggtcatgcga aataaataag gtcatgccta
     6181 gaaggatatt gtccccgcac aagaaccttc gagtgaactc gtgactactg cctcctggtc
     6241 ttctgtgtgg gcatcccctt ctttcttgca gcatccagga cccacttctg tccctgctgg
     6301 tctgtttctt tctttctttc tttctttctt tctttctttc ttgttttctt ccgtttttcc
     6361 ccttgagttc tctggggttg ggcctgggat tttgggtacg cctacagact gtccgcctct
     6421 gcaatcgacc gtcgtgcttc ccgcgccaga acaaggaagc ccacgcgagc cgcgtaggtg
     6481 gctcgcgtgg aagacccggg ttcgagtccc gctgggagaa ttcctcctag gcaagtggag
     6541 tcatctcggt caggatttct cagccggaag ccgcgccaac acctctcatc gtgggcggca
     6601 agttgtcccc ggggcttctt tgaccgtgcg gggccagccg acgctttctc tgaccgtttc
     6661 cgaagcccag tttcctttcc taaggagcct tggggatcct gcactcgcgt ctctgaaacc
     6721 caacccacga cagaacccag ccgcctgggc cagcttagca atgcgcccgc gccccgccct
     6781 ccgcgtgacg cgaacgccgg cggaccaatc agagagcgcg gcgcgagccc cacgcgcctg
     6841 tggccccacc ctccaagctt aagaaagggc gcgcgggcag agcccgggcc agaacgccgc
     6901 gcaaagcgga gagcgggttt ttgcgacggc aggcggcgct tgtgctgccg tttccgtgct
     6961 gcacgcggtt ccctcgagtg ccgcgctcgg acagacgtag ccggccgcga aggcgcccag
     7021 acggcaaacc agcgacccat gctgaagtga gcacacaggt cagcgaggat atcaagatct
     7081 ggcctcggcg gccaagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc
     7141 aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag
     7201 cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac
     7261 gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca
     7321 gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat
     7381 agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca
     7441 gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc
     7501 gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg
     7561 atcatacaaa agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg
     7621 tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag
     7681 agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg
     7741 cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac
     7801 cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac
     7861 cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc
     7921 atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct
     7981 gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac
     8041 gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt
     8101 gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa
     8161 acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag
     8221 gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg
     8281 aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac
     8341 cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc
     8401 tactgggacg a
//
