LOCUS       sequence-403383-        7911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for Igf1 enhancer 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, 
            Spiegel I
  TITLE     Single genomic enhancers drive experience-dependent GABAergic 
            plasticity to maintain sensory processing in the adult cortex.
  JOURNAL   Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026.
  PUBMED    37354902
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Jun 20:S0896-6273(23)00399-9. doi: 
            10.1016/j.neuron.2023.05.026."
COMMENT     Sequence Label: pGL4.11_Nued2_E4
FEATURES             Location/Qualifiers
     source          1..7911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             370..489
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    541..662
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(552..571)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     606..625
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(910..927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1081..1669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1161..1180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1869..2660,2661..2729))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2834..2882
                     /note="synthetic polyadenylation signal"
     misc_feature    2896..2987
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2936..2955
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      6622..6631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6628..7911,1..366)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
       61 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
      121 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
      181 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
      241 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
      301 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
      361 gccgtgaatt ctcacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
      421 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
      481 atcaacgtct aaggccgcga ctctagagtc ggggcggccg gccgcttcga gcagacatga
      541 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      601 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      661 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
      721 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
      781 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
      841 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
      901 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1501 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1741 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1801 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     1861 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     1921 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     1981 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     2041 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     2101 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     2161 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     2221 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     2281 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     2341 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     2401 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     2461 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     2521 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     2581 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     2641 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     2701 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     2761 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     2821 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     2881 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     2941 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc
     3001 ggtacctgag ctcgctagct tcctggcttg tagtgacctc gtctattgct agaaatcaga
     3061 aggtaccact gagccttcat ttgtttggtg aaatcatgga gttacattaa caagccgttt
     3121 ccccaaacta tggctctcta cccacttgta atacacccgt gcacaggaac attttctgaa
     3181 agtcaatgtt tcctatcact tgctccaaaa ttaaaccaga gatacatcat tcaggaagaa
     3241 gccagatgca ctgaagaaag aaaaacctgc tttatcagaa ggggcaagct tctctcacat
     3301 cccatagcaa tttaaattca tttgccggaa tgttaaatac aaccagtctt ttagttgggt
     3361 cccttcacat aaacaccaag ttagcataaa tgcaaaatag ttcacatgat taaatcttta
     3421 taacaccata atttatctta tgtacacaca cacacacaga gaaagagaga gagagagaga
     3481 gagagagaga tcttttactt gctgatactt ctacattttt tctggagttc tcaactctca
     3541 tcatctctct aaggaagctg gcttgtattg ccccagactc ttagtgtatt tagttctaaa
     3601 ggatagtata cagaataaag ggaaatttta attttgtttt taataggacc tatataattc
     3661 aggcctagct ctggtggaga gaaggggagg agaggtccat tagcatttat tgtgcacctg
     3721 ctttatgcta aacatgtctg gaaacagtgt ctcacacagc tggtttgttt gtcttggaca
     3781 agctgctgaa gtgagaaaaa aaaagttttt gcttggctgc atgtgaaact gtttcataac
     3841 cgttcaataa gtgcattgaa ggtgggtgcc agctccgagg agcgctgctc ctaaaggacc
     3901 tccacttgta gactattgtc aagggtaacc agcataatta aattaagaca atgagaaaag
     3961 caacagatgc aactgagacc aagtccctga gtgcttttgt tagtcactat cattgtctgc
     4021 aacaaagaag tcacatgtga gagactggga agggagccaa gtgcaaacta gactgcacag
     4081 ctggttttaa atgggttctc tgagcctgtg tgcctggaag catgctactt agagcagtgg
     4141 atgaggtggg tgagcttaag cgcgtttaaa cgtgtggcgc gccagctagt aacagttggc
     4201 attgggacta gaaggcctca ttcctaaacc agcagatttt tccattgtac caggaaaaca
     4261 aaactgcaaa tagcataagt cctctgattt tactgtctcc agagaaaatc atatcctcaa
     4321 tgcctgggtc tttccatctg gaatttggca actctgctga tcccttctca tagtgggaac
     4381 tcttgtagga agtctaagag caattatttt ggggatatat gctattttta tgtcttcttg
     4441 tacatcacct tctttggtca ggaggaaatt agaattctgg aaatacgttt aattattaaa
     4501 aggacaagcc ttggcttcaa gtagttgcaa atatgaatta ttagtccatg tcatgaaagg
     4561 gctggagaac tacaaagaaa caatgataat ctatttgtca cacccaatta agatctgccc
     4621 tggcctggga gtaaaagagc attaaatctg tgagtggtaa cttcaagcag catgtctata
     4681 tttgggtaat tctgaacatg cctggatttg gtaccttaca gcatcctcta aaaatgtact
     4741 actttagaaa gtgttttgag attttgttgc cttcgtgatt gcagtttgtg tgtgtgtgtg
     4801 tgtgtggggg gtgttatttg agcttttgag tttccatgta gccaaagaaa gaaagtctag
     4861 gaagaaatgg tagactcaag gtcaaattca acaaggggaa acttctttgt ggtgagtcta
     4921 gacaacaaag ccattagccc agtgttcacc cccctgccta caccctacct tttccattga
     4981 tgaatattct tacctgagcc atcctcgctt ctgagtcctg ctctgggtcc tgtggagact
     5041 gaatatcctg tctgtagttt ttaatccttg aagagatttt tcatgaatag aactttcctt
     5101 ataatttagc aacactgtcc ttgaaaaccc ccacaactat ttacataaca gaagctattg
     5161 tttgaagtgc aaaacaggtc agaatttggt tcccagagat taatcactgc agtctatttt
     5221 gtattatcat aataatttca tttattttga tacaatttgt tttcagaatc aaccccagct
     5281 tctttgcaca agtgacaaat ccatttgttt ctggtagaat gggtaatgtg attaatgacc
     5341 ttgcaacagg atttctttaa aaagggggaa cttttttttt ttcattttta attcacagta
     5401 aaccagctga gtctgttaag tgtgtaactg ttctgaactt gtaagccatg ggtaagattt
     5461 tgaaataccc cctcccaatc ttttgcgggg tttctgggta ttaatattag gctttctcca
     5521 agaatctgat gatactagtc tccaggtgta aagtggactt gttggattcc cgcattaaaa
     5581 agaacggggc cccagtcttt gccatggccc catcatgttt attccggctg gatgttctca
     5641 tcaaaccgag gtcatgcgaa ataaataagg tcatgcctag aaggatattg tccccgcaca
     5701 agaaccttcg agtgaactcg tgactactgc ctcctggtct tctgtgtggg catccccttc
     5761 tttcttgcag catccaggac ccacttctgt ccctgctggt ctgtttcttt ctttctttct
     5821 ttctttcttt ctttctttct tgttttcttc cgtttttccc cttgagttct ctggggttgg
     5881 gcctgggatt ttgggtacgc ctacagactg tccgcctctg caatcgaccg tcgtgcttcc
     5941 cgcgccagaa caaggaagcc cacgcgagcc gcgtaggtgg ctcgcgtgga agacccgggt
     6001 tcgagtcccg ctgggagaat tcctcctagg caagtggagt catctcggtc aggatttctc
     6061 agccggaagc cgcgccaaca cctctcatcg tgggcggcaa gttgtccccg gggcttcttt
     6121 gaccgtgcgg ggccagccga cgctttctct gaccgtttcc gaagcccagt ttcctttcct
     6181 aaggagcctt ggggatcctg cactcgcgtc tctgaaaccc aacccacgac agaacccagc
     6241 cgcctgggcc agcttagcaa tgcgcccgcg ccccgccctc cgcgtgacgc gaacgccggc
     6301 ggaccaatca gagagcgcgg cgcgagcccc acgcgcctgt ggccccaccc tccaagctta
     6361 agaaagggcg cgcgggcaga gcccgggcca gaacgccgcg caaagcggag agcgggtttt
     6421 tgcgacggca ggcggcgctt gtgctgccgt ttccgtgctg cacgcggttc cctcgagtgc
     6481 cgcgctcgga cagacgtagc cggccgcgaa ggcgcccaga cggcaaacca gcgacccatg
     6541 ctgaagtgag cacacaggtc agcgaggata tcaagatctg gcctcggcgg ccaagcttgg
     6601 caatccggta ctgttggtaa agccaccatg gaagatgcca aaaacattaa gaagggccca
     6661 gcgccattct acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag
     6721 cgctacgccc tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt
     6781 acctacgccg agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg
     6841 ctgaatacaa accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc
     6901 gtgttgggtg ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag
     6961 cgcgagctgc tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa
     7021 gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc
     7081 atggatagca agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat
     7141 ttgccacccg gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc
     7201 atcgccctga tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg
     7261 caccgcaccg cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc
     7321 atccccgaca ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc
     7381 acgctgggct acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag
     7441 ctattcttgc gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta
     7501 tttagcttct tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag
     7561 atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc
     7621 cacctaccag gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc
     7681 acccccgaag gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct
     7741 aaggtggtgg acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc
     7801 gtccgtggcc ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc
     7861 atcgacaagg acggctggct gcacagcggc gacatcgcct actgggacga g
//
